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Protein

Enolase-phosphatase E1

Gene

Enoph1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (Mri1)
  2. Methylthioribulose-1-phosphate dehydratase (Apip)
  3. Enolase-phosphatase E1 (Enoph1)
  4. Enolase-phosphatase E1 (Enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumUniRule annotation1
Metal bindingi18Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei184SubstrateUniRule annotation1
Metal bindingi209MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00876
UPA00904; UER00877

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
MASA homologUniRule annotation
Gene namesi
Name:Enoph1
Synonyms:Masa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1915120 Enoph1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002540081 – 257Enolase-phosphatase E1Add BLAST257

Proteomic databases

EPDiQ8BGB7
PaxDbiQ8BGB7
PeptideAtlasiQ8BGB7
PRIDEiQ8BGB7

PTM databases

iPTMnetiQ8BGB7
PhosphoSitePlusiQ8BGB7

Expressioni

Gene expression databases

BgeeiENSMUSG00000029326
CleanExiMM_ENOPH1
GenevisibleiQ8BGB7 MM

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi212497, 1 interactor
IntActiQ8BGB7, 1 interactor
MINTiQ8BGB7
STRINGi10090.ENSMUSP00000031268

Structurei

3D structure databases

ProteinModelPortaliQ8BGB7
SMRiQ8BGB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 151Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2630 Eukaryota
COG4229 LUCA
GeneTreeiENSGT00440000039914
HOGENOMiHOG000237286
HOVERGENiHBG054539
InParanoidiQ8BGB7
KOiK09880
OMAiHMWRAAY
OrthoDBiEOG091G0IX4
PhylomeDBiQ8BGB7
TreeFamiTF105939

Family and domain databases

CDDicd01629 HAD_EP, 1 hit
Gene3Di3.40.50.1000, 2 hits
HAMAPiMF_01681 Salvage_MtnC, 1 hit
MF_03117 Salvage_MtnC_euk, 1 hit
InterProiView protein in InterPro
IPR023943 Enolase-ppase_E1
IPR027511 ENOPH1_eukaryotes
IPR036412 HAD-like_sf
IPR006439 HAD-SF_hydro_IA
IPR023214 HAD_sf
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01691 enolase-ppase, 1 hit
TIGR01549 HAD-SF-IA-v1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGB7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVVSVPAEV TVILLDIEGT TTPIAFVKDV LFPYIKENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPVASG SDLQQMIQAV VDNVYWQMSH
110 120 130 140 150
DRKTTALKQL QGHMWKAAFT AGRMKAEFFA DVVPAVRRWR EAGMKVYIYS
160 170 180 190 200
SGSVEAQKLL FGHSTEGDIL ELIDGHFDTK IGHKVDSESY RKIADSIGCS
210 220 230 240 250
TNNILFLTDV TVEASAAEEA DVHVAVVVRP GNAGLTDDEK TYYNLITSFS

ELYLPST
Length:257
Mass (Da):28,600
Last modified:March 1, 2003 - v1
Checksum:iAD64B1C70976C344
GO
Isoform 2 (identifier: Q8BGB7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-85: Missing.

Show »
Length:226
Mass (Da):25,359
Checksum:iC8DBC7513DB08943
GO

Sequence cautioni

The sequence BAE30883 differs from that shown. Reason: Frameshift at position 220.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56V → I in AAH21429 (PubMed:15489334).Curated1
Sequence conflicti74P → L in AAH21429 (PubMed:15489334).Curated1
Sequence conflicti152G → R in BAB26606 (PubMed:16141072).Curated1
Sequence conflicti236T → N in BAE35014 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02116155 – 85Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009954 mRNA Translation: BAB26606.1
AK079062 mRNA Translation: BAC37520.1
AK080714 mRNA Translation: BAC37988.1
AK082747 mRNA Translation: BAC38597.1
AK152021 mRNA Translation: BAE30883.1 Frameshift.
AK159356 mRNA Translation: BAE35014.1
AK167575 mRNA Translation: BAE39638.1
AK168696 mRNA Translation: BAE40540.1
BC021429 mRNA Translation: AAH21429.1
CCDSiCCDS39183.1 [Q8BGB7-1]
RefSeqiNP_001156507.1, NM_001163035.1 [Q8BGB7-1]
NP_080697.2, NM_026421.3 [Q8BGB7-1]
UniGeneiMm.11311

Genome annotation databases

EnsembliENSMUST00000031268; ENSMUSP00000031268; ENSMUSG00000029326 [Q8BGB7-1]
ENSMUST00000169390; ENSMUSP00000129704; ENSMUSG00000029326 [Q8BGB7-1]
GeneIDi67870
KEGGimmu:67870
UCSCiuc008yha.2 mouse [Q8BGB7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiENOPH_MOUSE
AccessioniPrimary (citable) accession number: Q8BGB7
Secondary accession number(s): Q3TGK8
, Q3TXA2, Q3U8Y4, Q8VC92, Q9D6U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health