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Q8BGB7

- ENOPH_MOUSE

UniProt

Q8BGB7 - ENOPH_MOUSE

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Protein

Enolase-phosphatase E1

Gene
Enoph1, Masa
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.UniRule annotation

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Magnesium By similarity
Metal bindingi18 – 181Magnesium; via carbonyl oxygen By similarity
Binding sitei184 – 1841Substrate By similarity
Metal bindingi209 – 2091Magnesium By similarity

GO - Molecular functioni

  1. acireductone synthase activity Source: UniProtKB
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. L-methionine salvage from methylthioadenosine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199099. Methionine salvage pathway.
UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1 (EC:3.1.3.77)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
MASA homolog
Gene namesi
Name:Enoph1
Synonyms:Masa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1915120. Enoph1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Enolase-phosphatase E1UniRule annotationPRO_0000254008Add
BLAST

Proteomic databases

MaxQBiQ8BGB7.
PaxDbiQ8BGB7.
PRIDEiQ8BGB7.

Expressioni

Gene expression databases

BgeeiQ8BGB7.
CleanExiMM_ENOPH1.
GenevestigatoriQ8BGB7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi212497. 1 interaction.
IntActiQ8BGB7. 1 interaction.
MINTiMINT-4110231.
STRINGi10090.ENSMUSP00000031268.

Structurei

3D structure databases

ProteinModelPortaliQ8BGB7.
SMRiQ8BGB7. Positions 4-254.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1512Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4229.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
HOVERGENiHBG054539.
InParanoidiQ8BGB7.
KOiK09880.
OMAiDTHVGHK.
OrthoDBiEOG7BP83J.
PhylomeDBiQ8BGB7.
TreeFamiTF105939.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_01681. Salvage_MtnC.
MF_03117. Salvage_MtnC_euk.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BGB7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVVVSVPAEV TVILLDIEGT TTPIAFVKDV LFPYIKENVK EYLQTHWEEE    50
ECQQDVSLLR KQAEEDAHLD GAVPIPVASG SDLQQMIQAV VDNVYWQMSH 100
DRKTTALKQL QGHMWKAAFT AGRMKAEFFA DVVPAVRRWR EAGMKVYIYS 150
SGSVEAQKLL FGHSTEGDIL ELIDGHFDTK IGHKVDSESY RKIADSIGCS 200
TNNILFLTDV TVEASAAEEA DVHVAVVVRP GNAGLTDDEK TYYNLITSFS 250
ELYLPST 257
Length:257
Mass (Da):28,600
Last modified:March 1, 2003 - v1
Checksum:iAD64B1C70976C344
GO
Isoform 2 (identifier: Q8BGB7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-85: Missing.

Show »
Length:226
Mass (Da):25,359
Checksum:iC8DBC7513DB08943
GO

Sequence cautioni

The sequence BAE30883.1 differs from that shown. Reason: Frameshift at position 220.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei55 – 8531Missing in isoform 2. VSP_021161Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561V → I in AAH21429. 1 Publication
Sequence conflicti74 – 741P → L in AAH21429. 1 Publication
Sequence conflicti152 – 1521G → R in BAB26606. 1 Publication
Sequence conflicti236 – 2361T → N in BAE35014. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009954 mRNA. Translation: BAB26606.1.
AK079062 mRNA. Translation: BAC37520.1.
AK080714 mRNA. Translation: BAC37988.1.
AK082747 mRNA. Translation: BAC38597.1.
AK152021 mRNA. Translation: BAE30883.1. Frameshift.
AK159356 mRNA. Translation: BAE35014.1.
AK167575 mRNA. Translation: BAE39638.1.
AK168696 mRNA. Translation: BAE40540.1.
BC021429 mRNA. Translation: AAH21429.1.
CCDSiCCDS39183.1. [Q8BGB7-1]
RefSeqiNP_001156507.1. NM_001163035.1. [Q8BGB7-1]
NP_080697.2. NM_026421.3. [Q8BGB7-1]
UniGeneiMm.11311.

Genome annotation databases

EnsembliENSMUST00000031268; ENSMUSP00000031268; ENSMUSG00000029326. [Q8BGB7-1]
ENSMUST00000169390; ENSMUSP00000129704; ENSMUSG00000029326. [Q8BGB7-1]
GeneIDi67870.
KEGGimmu:67870.
UCSCiuc008yha.2. mouse. [Q8BGB7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009954 mRNA. Translation: BAB26606.1 .
AK079062 mRNA. Translation: BAC37520.1 .
AK080714 mRNA. Translation: BAC37988.1 .
AK082747 mRNA. Translation: BAC38597.1 .
AK152021 mRNA. Translation: BAE30883.1 . Frameshift.
AK159356 mRNA. Translation: BAE35014.1 .
AK167575 mRNA. Translation: BAE39638.1 .
AK168696 mRNA. Translation: BAE40540.1 .
BC021429 mRNA. Translation: AAH21429.1 .
CCDSi CCDS39183.1. [Q8BGB7-1 ]
RefSeqi NP_001156507.1. NM_001163035.1. [Q8BGB7-1 ]
NP_080697.2. NM_026421.3. [Q8BGB7-1 ]
UniGenei Mm.11311.

3D structure databases

ProteinModelPortali Q8BGB7.
SMRi Q8BGB7. Positions 4-254.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 212497. 1 interaction.
IntActi Q8BGB7. 1 interaction.
MINTi MINT-4110231.
STRINGi 10090.ENSMUSP00000031268.

Proteomic databases

MaxQBi Q8BGB7.
PaxDbi Q8BGB7.
PRIDEi Q8BGB7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031268 ; ENSMUSP00000031268 ; ENSMUSG00000029326 . [Q8BGB7-1 ]
ENSMUST00000169390 ; ENSMUSP00000129704 ; ENSMUSG00000029326 . [Q8BGB7-1 ]
GeneIDi 67870.
KEGGi mmu:67870.
UCSCi uc008yha.2. mouse. [Q8BGB7-1 ]

Organism-specific databases

CTDi 58478.
MGIi MGI:1915120. Enoph1.

Phylogenomic databases

eggNOGi COG4229.
GeneTreei ENSGT00440000039914.
HOGENOMi HOG000237286.
HOVERGENi HBG054539.
InParanoidi Q8BGB7.
KOi K09880.
OMAi DTHVGHK.
OrthoDBi EOG7BP83J.
PhylomeDBi Q8BGB7.
TreeFami TF105939.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00876 .
UPA00904 ; UER00877 .
Reactomei REACT_199099. Methionine salvage pathway.

Miscellaneous databases

ChiTaRSi ENOPH1. mouse.
NextBioi 325783.
PROi Q8BGB7.
SOURCEi Search...

Gene expression databases

Bgeei Q8BGB7.
CleanExi MM_ENOPH1.
Genevestigatori Q8BGB7.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
HAMAPi MF_01681. Salvage_MtnC.
MF_03117. Salvage_MtnC_euk.
InterProi IPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view ]
Pfami PF13419. HAD_2. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Bone marrow macrophage, Diencephalon, Embryonic kidney, Osteoclast, Placenta, Retina and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiENOPH_MOUSE
AccessioniPrimary (citable) accession number: Q8BGB7
Secondary accession number(s): Q3TGK8
, Q3TXA2, Q3U8Y4, Q8VC92, Q9D6U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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