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Protein

Acyl-coenzyme A synthetase ACSM5, mitochondrial

Gene

Acsm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) (By similarity).By similarity

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei454 – 4541ATPBy similarity
Binding sitei469 – 4691ATPBy similarity
Binding sitei565 – 5651ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi229 – 2379ATPBy similarity
Nucleotide bindingi367 – 3726ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM5, mitochondrial (EC:6.2.1.2)
Gene namesi
Name:Acsm5
Synonyms:Macs3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444086. Acsm5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionSequence analysisAdd
BLAST
Chaini23 – 578556Acyl-coenzyme A synthetase ACSM5, mitochondrialPRO_0000306102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei96 – 961N6-acetyllysine; alternateCombined sources
Modified residuei96 – 961N6-succinyllysine; alternateCombined sources
Modified residuei151 – 1511N6-acetyllysineCombined sources
Modified residuei302 – 3021N6-acetyllysine; alternateCombined sources
Modified residuei302 – 3021N6-succinyllysine; alternateCombined sources
Modified residuei335 – 3351N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BGA8.
PaxDbiQ8BGA8.
PeptideAtlasiQ8BGA8.
PRIDEiQ8BGA8.

PTM databases

iPTMnetiQ8BGA8.
PhosphoSiteiQ8BGA8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030972.
CleanExiMM_ACSM5.
ExpressionAtlasiQ8BGA8. baseline and differential.
GenevisibleiQ8BGA8. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BGA8. 2 interactions.
MINTiMINT-1851215.
STRINGi10090.ENSMUSP00000063416.

Structurei

3D structure databases

ProteinModelPortaliQ8BGA8.
SMRiQ8BGA8. Positions 44-576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 327Poly-Pro

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ8BGA8.
KOiK01896.
OMAiCFFNCYL.
OrthoDBiEOG091G03NW.
PhylomeDBiQ8BGA8.
TreeFamiTF354287.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLWLRGLAC QALRSSWGVC RIHTQPPPPP IPEVVATWEA ISLGRQPVPE
60 70 80 90 100
YFNFAHDVLD VWSQLEKTGH RPPNPAFWWV NGSGTEVKWT FEELGKQSRK
110 120 130 140 150
AANVLEGVCG LQPGDRMMLV LPRLPDWWLI SVACMRTGVV MIPGVSQLTA
160 170 180 190 200
KDLKYRLQAA RAKSIVTSDA LAPQVDAISA DCPSLQTKLL VSDTSRPGWI
210 220 230 240 250
NFRELLRAAS PEHNCVRTRS GDSVAIYFTS GTTGAPKMVE HSQSSYGLGF
260 270 280 290 300
VASGRRWMAL TESDIFWNTT DTGWVKAAWT LFSAWSNGAC IFVHELPRVD
310 320 330 340 350
AKTILNTLCR FPITTLCCVP TLFRLLVQED LTRYKFQCLR HCLTGGEALN
360 370 380 390 400
PDVRDKWKSQ TGLELHEGYG QSETVVICGN SRNSTIKSGS MGKASPPYDV
410 420 430 440 450
QIVDEEGNVL PPGKEGNIAV RIKPTRPFCF FNCYLDNPEK TAASEQGDFY
460 470 480 490 500
ITGDRAHMDE DGYFWFLGRN DDVINSSSYR IGPVEVESAL AEHPAVLESA
510 520 530 540 550
VVSSPDPIRG EVVKAFIVLS PAYASHDPEA LTRELQEHVK TVTAPYKYPR
560 570
KVAFISELPK TVSGKILRSK LRNQEWGR
Length:578
Mass (Da):64,328
Last modified:March 1, 2003 - v1
Checksum:iAF6BE6B148FEC91E
GO
Isoform 2 (identifier: Q8BGA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     307-310: TLCR → VRRKI
     311-578: Missing.

Show »
Length:311
Mass (Da):34,628
Checksum:i2BF1AD357E41B94A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61R → T in BAC34084 (PubMed:16141072).Curated
Sequence conflicti59 – 591L → S in BAC34084 (PubMed:16141072).Curated
Sequence conflicti96 – 961K → N in BAC34084 (PubMed:16141072).Curated
Sequence conflicti114 – 1141G → E in BAC30656 (PubMed:16141072).Curated
Sequence conflicti253 – 2531S → G in BAC34167 (PubMed:16141072).Curated
Sequence conflicti253 – 2531S → G in BAE28980 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei307 – 3104TLCR → VRRKI in isoform 2. 2 PublicationsVSP_028399
Alternative sequencei311 – 578268Missing in isoform 2. 2 PublicationsVSP_028400Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040650 mRNA. Translation: BAC30656.1.
AK050132 mRNA. Translation: BAC34084.1.
AK050219 mRNA. Translation: BAC34130.1.
AK050288 mRNA. Translation: BAC34167.1.
AK050458 mRNA. Translation: BAC34268.1.
AK080219 mRNA. Translation: BAC37850.1.
AK149591 mRNA. Translation: BAE28980.1.
BC095985 mRNA. Translation: AAH95985.1.
CCDSiCCDS40106.1. [Q8BGA8-1]
RefSeqiNP_848873.1. NM_178758.3. [Q8BGA8-1]
UniGeneiMm.185183.

Genome annotation databases

EnsembliENSMUST00000066465; ENSMUSP00000063416; ENSMUSG00000030972. [Q8BGA8-1]
ENSMUST00000207387; ENSMUSP00000146357; ENSMUSG00000030972. [Q8BGA8-2]
ENSMUST00000207440; ENSMUSP00000146938; ENSMUSG00000030972. [Q8BGA8-1]
GeneIDi272428.
KEGGimmu:272428.
UCSCiuc009jle.1. mouse. [Q8BGA8-2]
uc009jlf.1. mouse. [Q8BGA8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040650 mRNA. Translation: BAC30656.1.
AK050132 mRNA. Translation: BAC34084.1.
AK050219 mRNA. Translation: BAC34130.1.
AK050288 mRNA. Translation: BAC34167.1.
AK050458 mRNA. Translation: BAC34268.1.
AK080219 mRNA. Translation: BAC37850.1.
AK149591 mRNA. Translation: BAE28980.1.
BC095985 mRNA. Translation: AAH95985.1.
CCDSiCCDS40106.1. [Q8BGA8-1]
RefSeqiNP_848873.1. NM_178758.3. [Q8BGA8-1]
UniGeneiMm.185183.

3D structure databases

ProteinModelPortaliQ8BGA8.
SMRiQ8BGA8. Positions 44-576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BGA8. 2 interactions.
MINTiMINT-1851215.
STRINGi10090.ENSMUSP00000063416.

PTM databases

iPTMnetiQ8BGA8.
PhosphoSiteiQ8BGA8.

Proteomic databases

MaxQBiQ8BGA8.
PaxDbiQ8BGA8.
PeptideAtlasiQ8BGA8.
PRIDEiQ8BGA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066465; ENSMUSP00000063416; ENSMUSG00000030972. [Q8BGA8-1]
ENSMUST00000207387; ENSMUSP00000146357; ENSMUSG00000030972. [Q8BGA8-2]
ENSMUST00000207440; ENSMUSP00000146938; ENSMUSG00000030972. [Q8BGA8-1]
GeneIDi272428.
KEGGimmu:272428.
UCSCiuc009jle.1. mouse. [Q8BGA8-2]
uc009jlf.1. mouse. [Q8BGA8-1]

Organism-specific databases

CTDi54988.
MGIiMGI:2444086. Acsm5.

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ8BGA8.
KOiK01896.
OMAiCFFNCYL.
OrthoDBiEOG091G03NW.
PhylomeDBiQ8BGA8.
TreeFamiTF354287.

Miscellaneous databases

PROiQ8BGA8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030972.
CleanExiMM_ACSM5.
ExpressionAtlasiQ8BGA8. baseline and differential.
GenevisibleiQ8BGA8. MM.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSM5_MOUSE
AccessioniPrimary (citable) accession number: Q8BGA8
Secondary accession number(s): Q8BJS0
, Q8BM02, Q8BWQ8, Q8BWS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.