Reviewed, UniProtKB/Swiss-Prot Q8BGA8 (ACSM5_MOUSE)

Names and origin

Protein names

Recommended name:
    Acyl-coenzyme A synthetase ACSM5, mitochondrial
    EC=6.2.1.2

Gene names

Name:	Acsm5
Synonyms:	Macs3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) By similarity.
Catalytic activity	ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BGA8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BGA8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     307-310: TLCR → VRRKI
     311-578: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 578	556	Acyl-coenzyme A synthetase ACSM5, mitochondrial		PRO_0000306102
Regions
	Nucleotide binding	229 – 237	9	ATP By similarity
	Nucleotide binding	367 – 372	6	ATP By similarity
	Compositional bias	26 – 32	7	Poly-Pro
Sites
	Binding site	454	1	ATP By similarity
	Binding site	469	1	ATP By similarity
	Binding site	565	1	ATP By similarity
Natural variations
	Alternative sequence	307 – 310	4	TLCR → VRRKI in isoform 2.		VSP_028399
	Alternative sequence	311 – 578	268	Missing in isoform 2.		VSP_028400
Experimental info
	Sequence conflict	6	1	R → T in BAC34084. Ref.1
	Sequence conflict	59	1	L → S in BAC34084. Ref.1
	Sequence conflict	96	1	K → N in BAC34084. Ref.1
	Sequence conflict	114	1	G → E in BAC30656. Ref.1
	Sequence conflict	253	1	S → G in BAC34167. Ref.1
	Sequence conflict	253	1	S → G in BAE28980. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: AF6BE6B148FEC91E	FASTA	578	64,328
	10 20 30 40 50 60 MRLWLRGLAC QALRSSWGVC RIHTQPPPPP IPEVVATWEA ISLGRQPVPE YFNFAHDVLD 70 80 90 100 110 120 VWSQLEKTGH RPPNPAFWWV NGSGTEVKWT FEELGKQSRK AANVLEGVCG LQPGDRMMLV 130 140 150 160 170 180 LPRLPDWWLI SVACMRTGVV MIPGVSQLTA KDLKYRLQAA RAKSIVTSDA LAPQVDAISA 190 200 210 220 230 240 DCPSLQTKLL VSDTSRPGWI NFRELLRAAS PEHNCVRTRS GDSVAIYFTS GTTGAPKMVE 250 260 270 280 290 300 HSQSSYGLGF VASGRRWMAL TESDIFWNTT DTGWVKAAWT LFSAWSNGAC IFVHELPRVD 310 320 330 340 350 360 AKTILNTLCR FPITTLCCVP TLFRLLVQED LTRYKFQCLR HCLTGGEALN PDVRDKWKSQ 370 380 390 400 410 420 TGLELHEGYG QSETVVICGN SRNSTIKSGS MGKASPPYDV QIVDEEGNVL PPGKEGNIAV 430 440 450 460 470 480 RIKPTRPFCF FNCYLDNPEK TAASEQGDFY ITGDRAHMDE DGYFWFLGRN DDVINSSSYR 490 500 510 520 530 540 IGPVEVESAL AEHPAVLESA VVSSPDPIRG EVVKAFIVLS PAYASHDPEA LTRELQEHVK 550 560 570 TVTAPYKYPR KVAFISELPK TVSGKILRSK LRNQEWGR
	Isoform 2. Checksum: 2BF1AD357E41B94A	FASTA	311	34,628
	10 20 30 40 50 60 MRLWLRGLAC QALRSSWGVC RIHTQPPPPP IPEVVATWEA ISLGRQPVPE YFNFAHDVLD 70 80 90 100 110 120 VWSQLEKTGH RPPNPAFWWV NGSGTEVKWT FEELGKQSRK AANVLEGVCG LQPGDRMMLV 130 140 150 160 170 180 LPRLPDWWLI SVACMRTGVV MIPGVSQLTA KDLKYRLQAA RAKSIVTSDA LAPQVDAISA 190 200 210 220 230 240 DCPSLQTKLL VSDTSRPGWI NFRELLRAAS PEHNCVRTRS GDSVAIYFTS GTTGAPKMVE 250 260 270 280 290 300 HSQSSYGLGF VASGRRWMAL TESDIFWNTT DTGWVKAAWT LFSAWSNGAC IFVHELPRVD 310 AKTILNVRRK I

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Aorta, Liver and Vein.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK040650 mRNA. Translation: BAC30656.1. AK050132 mRNA. Translation: BAC34084.1. AK050219 mRNA. Translation: BAC34130.1. AK050288 mRNA. Translation: BAC34167.1. AK050458 mRNA. Translation: BAC34268.1. AK080219 mRNA. Translation: BAC37850.1. AK149591 mRNA. Translation: BAE28980.1. BC095985 mRNA. Translation: AAH95985.1.
IPI	IPI00221556. IPI00867824.
RefSeq	NP_848873.1.
UniGene	Mm.185183
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
PTM databases
PhosphoSite	Q8BGA8.
Proteomic databases
PRIDE	Q8BGA8.
Genome annotation databases
Ensembl	ENSMUST00000066465; ENSMUSP00000063416; ENSMUSG00000030972; Mus musculus. [Genome view]
GeneID	272428.
KEGG	mmu:272428.
UCSC	uc009jle.1. mouse. uc009jlf.1. mouse.
Organism-specific databases
CTD	272428.
MGI	MGI:2444086. Acsm5.
Phylogenomic databases
HOVERGEN	Q8BGA8.
OMA	HELPRVD.
Enzyme and pathway databases
BRENDA	6.2.1.2. 244.
Gene expression databases
ArrayExpress	Q8BGA8.
Bgee	Q8BGA8.
CleanEx	MM_ACSM5.
Genevestigator	Q8BGA8.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	393590.
SOURCE	Search...

Entry information

Entry name

ACSM5_MOUSE

Accession

Primary (citable) accession number: Q8BGA8
Secondary accession number(s): Q8BJS0 , Q8BM02, Q8BWQ8, Q8BWS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)