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Protein

Protein phosphatase 1 regulatory subunit 12B

Gene

Ppp1r12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates myosin phosphatase activity. Augments Ca2+ sensitivity of the contractile apparatus (By similarity).By similarity

Caution

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5627123 RHO GTPases activate PAKs

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 12B
Alternative name(s):
Myosin phosphatase-targeting subunit 2
Short name:
Myosin phosphatase target subunit 2
Gene namesi
Name:Ppp1r12b
Synonyms:Mypt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1916417 Ppp1r12b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670291 – 976Protein phosphatase 1 regulatory subunit 12BAdd BLAST976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei444PhosphothreonineCombined sources1
Modified residuei502PhosphoserineCombined sources1
Modified residuei645PhosphothreonineBy similarity1
Modified residuei802PhosphothreonineCombined sources1
Modified residuei833PhosphoserineCombined sources1
Modified residuei941PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BG95
PaxDbiQ8BG95
PeptideAtlasiQ8BG95
PRIDEiQ8BG95

PTM databases

iPTMnetiQ8BG95
PhosphoSitePlusiQ8BG95

Expressioni

Gene expression databases

BgeeiENSMUSG00000073557
ExpressionAtlasiQ8BG95 baseline and differential
GenevisibleiQ8BG95 MM

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BG95 1 interactor.
STRINGi10090.ENSMUSP00000083633

Structurei

3D structure databases

ProteinModelPortaliQ8BG95
SMRiQ8BG95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati90 – 119ANK 2Add BLAST30
Repeati123 – 152ANK 3Add BLAST30
Repeati216 – 245ANK 4Add BLAST30
Repeati249 – 278ANK 5Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi336 – 379Glu-richAdd BLAST44

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0505 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00760000119141
HOGENOMiHOG000290648
HOVERGENiHBG052561
InParanoidiQ8BG95

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.202 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR017401 MYPT1/MYPT2/Mbs85
IPR031775 PRKG1_interact
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF15898 PRKG1_interact, 1 hit
PIRSFiPIRSF038141 PP1_12ABC_vert, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG95-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAELEHLGGK RAESARARRA EQLRRWRGSL TEQEPAERQG AGRQLQTRRG
60 70 80 90 100
SPRVRFEDGA VFLAACSSGD TDEVKKLLAR GADINTVNVD GLTALHQACI
110 120 130 140 150
DENLDMVKFL VENRANVNQQ DNEGWTPLHA AASCGYLNIA EYFISHGASV
160 170 180 190 200
GIVNSEGEVP SDLAEEPAMK DLLLEQVKKQ GVDLEQSRKE EEQQMLQDAR
210 220 230 240 250
QWLNSGRIED VRQARSGATA LHVAAAKGYS EVLRLLIQAG YELNVQDHDG
260 270 280 290 300
WTPLHAAAHW GVKEACSILA EALCDMDIRN KLGQTPFDVA DEGLVEHLEM
310 320 330 340 350
LQKKQDVLRS EKETRNKLIE SDLNSKFQSG LFKNKEKMLY EEEIPKSQDT
360 370 380 390 400
EEENKESSSS SSEEEEGEDE VSESETEKEA DKKPEATVNH SNSEIKSRIM
410 420 430 440 450
EQIPAPAQNT FSASSARRLS SLFNKAEEPK DESPSSWRLG LRKTGSHNML
460 470 480 490 500
SEVANSREAL RDRGSSIYRS SSSPRISALL DDKDKERENK SYFSMLVPRR
510 520 530 540 550
LSSTSDIEEK ENRESAVNLV RSGSHTRQLW RDEAKGSETP QTIAPSTYTS
560 570 580 590 600
TYLKRTPYKS QADSTAEKTA DSVSSSTPLC VITNRPAPST ANGVPAATVF
610 620 630 640 650
SSAGTDPSVE AREKRRSYLT PVRDEEAESL RKARSRQARQ TRRSTQGVTL
660 670 680 690 700
TDLQEAEKTF SRSRAERQAQ EQPGEKLEDP GGLEGSTKKQ EPSAAPTKGA
710 720 730 740 750
GEGRSLEEEP IYHRLRYPTQ PDKPTTPVSP SASRPSLYTG SHLLRTSRAS
760 770 780 790 800
GPDSENSETS THATAAKEMD TSEKGEADLD DQSSNRLSVR ERRRAKDRRR
810 820 830 840 850
GTGINFWTND EDETDVSEEV KEALHERLSR LESGGTNPTS SDSYSDRASA
860 870 880 890 900
RARREAREAR LASLTSRVEE DSNRDYKKLY ESALTENQKL KTKLQEAQLE
910 920 930 940 950
LADIKAKLEK MAQQKQEKTS DRSSVLEVEK RERRALERKM SEMEEEMKVL
960 970
TELKSDNQRL KDENGALIRV ISKLSK
Length:976
Mass (Da):109,049
Last modified:January 9, 2007 - v2
Checksum:i7694AC50766A8890
GO
Isoform 2 (identifier: Q8BG95-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-105: ACIDENLD → RNHKPRGS
     106-976: Missing.

Note: No experimental confirmation available.
Show »
Length:105
Mass (Da):11,616
Checksum:iC2CFC03472D7122A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00926098 – 105ACIDENLD → RNHKPRGS in isoform 2. 1 Publication8
Alternative sequenceiVSP_009261106 – 976Missing in isoform 2. 1 PublicationAdd BLAST871

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007727 mRNA Translation: BAB25216.1
AK042847 mRNA Translation: BAC31381.1
AK046012 mRNA Translation: BAC32572.1
AK046167 mRNA Translation: BAC32618.1
AK080855 mRNA Translation: BAC38046.1
AC117827 Genomic DNA No translation available.
AC131591 Genomic DNA No translation available.
RefSeqiXP_006529773.1, XM_006529710.2 [Q8BG95-1]
UniGeneiMm.188709
Mm.451578

Genome annotation databases

EnsembliENSMUST00000045665; ENSMUSP00000047463; ENSMUSG00000073557 [Q8BG95-1]
ENSMUST00000112163; ENSMUSP00000107788; ENSMUSG00000073557 [Q8BG95-2]
GeneIDi329251

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMYPT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BG95
Secondary accession number(s): Q8BXY7, Q9D8S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 9, 2007
Last modified: February 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome