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Protein

Protein phosphatase 1 regulatory subunit 12B

Gene

Ppp1r12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates myosin phosphatase activity. Augments Ca2+ sensitivity of the contractile apparatus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 12B
Alternative name(s):
Myosin phosphatase-targeting subunit 2
Short name:
Myosin phosphatase target subunit 2
Gene namesi
Name:Ppp1r12b
Synonyms:Mypt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1916417. Ppp1r12b.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Along actomyosin filaments and stress fibers.By similarity

GO - Cellular componenti

  • A band Source: UniProtKB
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 976976Protein phosphatase 1 regulatory subunit 12BPRO_0000067029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphoserineCombined sources
Modified residuei444 – 4441PhosphothreonineCombined sources
Modified residuei502 – 5021PhosphoserineCombined sources
Modified residuei802 – 8021PhosphothreonineCombined sources
Modified residuei833 – 8331PhosphoserineCombined sources
Modified residuei941 – 9411PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BG95.
MaxQBiQ8BG95.
PaxDbiQ8BG95.
PRIDEiQ8BG95.

PTM databases

iPTMnetiQ8BG95.
PhosphoSiteiQ8BG95.

Expressioni

Gene expression databases

BgeeiQ8BG95.
ExpressionAtlasiQ8BG95. baseline and differential.
GenevisibleiQ8BG95. MM.

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083633.

Structurei

3D structure databases

ProteinModelPortaliQ8BG95.
SMRiQ8BG95. Positions 11-336, 604-663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 8630ANK 1Add
BLAST
Repeati90 – 11930ANK 2Add
BLAST
Repeati123 – 15230ANK 3Add
BLAST
Repeati216 – 24530ANK 4Add
BLAST
Repeati249 – 27830ANK 5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 37944Glu-richAdd
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiQ8BG95.
OrthoDBiEOG7060Q5.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG95-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAELEHLGGK RAESARARRA EQLRRWRGSL TEQEPAERQG AGRQLQTRRG
60 70 80 90 100
SPRVRFEDGA VFLAACSSGD TDEVKKLLAR GADINTVNVD GLTALHQACI
110 120 130 140 150
DENLDMVKFL VENRANVNQQ DNEGWTPLHA AASCGYLNIA EYFISHGASV
160 170 180 190 200
GIVNSEGEVP SDLAEEPAMK DLLLEQVKKQ GVDLEQSRKE EEQQMLQDAR
210 220 230 240 250
QWLNSGRIED VRQARSGATA LHVAAAKGYS EVLRLLIQAG YELNVQDHDG
260 270 280 290 300
WTPLHAAAHW GVKEACSILA EALCDMDIRN KLGQTPFDVA DEGLVEHLEM
310 320 330 340 350
LQKKQDVLRS EKETRNKLIE SDLNSKFQSG LFKNKEKMLY EEEIPKSQDT
360 370 380 390 400
EEENKESSSS SSEEEEGEDE VSESETEKEA DKKPEATVNH SNSEIKSRIM
410 420 430 440 450
EQIPAPAQNT FSASSARRLS SLFNKAEEPK DESPSSWRLG LRKTGSHNML
460 470 480 490 500
SEVANSREAL RDRGSSIYRS SSSPRISALL DDKDKERENK SYFSMLVPRR
510 520 530 540 550
LSSTSDIEEK ENRESAVNLV RSGSHTRQLW RDEAKGSETP QTIAPSTYTS
560 570 580 590 600
TYLKRTPYKS QADSTAEKTA DSVSSSTPLC VITNRPAPST ANGVPAATVF
610 620 630 640 650
SSAGTDPSVE AREKRRSYLT PVRDEEAESL RKARSRQARQ TRRSTQGVTL
660 670 680 690 700
TDLQEAEKTF SRSRAERQAQ EQPGEKLEDP GGLEGSTKKQ EPSAAPTKGA
710 720 730 740 750
GEGRSLEEEP IYHRLRYPTQ PDKPTTPVSP SASRPSLYTG SHLLRTSRAS
760 770 780 790 800
GPDSENSETS THATAAKEMD TSEKGEADLD DQSSNRLSVR ERRRAKDRRR
810 820 830 840 850
GTGINFWTND EDETDVSEEV KEALHERLSR LESGGTNPTS SDSYSDRASA
860 870 880 890 900
RARREAREAR LASLTSRVEE DSNRDYKKLY ESALTENQKL KTKLQEAQLE
910 920 930 940 950
LADIKAKLEK MAQQKQEKTS DRSSVLEVEK RERRALERKM SEMEEEMKVL
960 970
TELKSDNQRL KDENGALIRV ISKLSK
Length:976
Mass (Da):109,049
Last modified:January 9, 2007 - v2
Checksum:i7694AC50766A8890
GO
Isoform 2 (identifier: Q8BG95-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-105: ACIDENLD → RNHKPRGS
     106-976: Missing.

Note: No experimental confirmation available.
Show »
Length:105
Mass (Da):11,616
Checksum:iC2CFC03472D7122A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 1058ACIDENLD → RNHKPRGS in isoform 2. 1 PublicationVSP_009260
Alternative sequencei106 – 976871Missing in isoform 2. 1 PublicationVSP_009261Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007727 mRNA. Translation: BAB25216.1.
AK042847 mRNA. Translation: BAC31381.1.
AK046012 mRNA. Translation: BAC32572.1.
AK046167 mRNA. Translation: BAC32618.1.
AK080855 mRNA. Translation: BAC38046.1.
AC117827 Genomic DNA. No translation available.
AC131591 Genomic DNA. No translation available.
RefSeqiXP_006529773.1. XM_006529710.1. [Q8BG95-1]
UniGeneiMm.188709.
Mm.451578.

Genome annotation databases

EnsembliENSMUST00000045665; ENSMUSP00000047463; ENSMUSG00000073557. [Q8BG95-1]
ENSMUST00000112163; ENSMUSP00000107788; ENSMUSG00000073557. [Q8BG95-2]
GeneIDi329251.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007727 mRNA. Translation: BAB25216.1.
AK042847 mRNA. Translation: BAC31381.1.
AK046012 mRNA. Translation: BAC32572.1.
AK046167 mRNA. Translation: BAC32618.1.
AK080855 mRNA. Translation: BAC38046.1.
AC117827 Genomic DNA. No translation available.
AC131591 Genomic DNA. No translation available.
RefSeqiXP_006529773.1. XM_006529710.1. [Q8BG95-1]
UniGeneiMm.188709.
Mm.451578.

3D structure databases

ProteinModelPortaliQ8BG95.
SMRiQ8BG95. Positions 11-336, 604-663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083633.

PTM databases

iPTMnetiQ8BG95.
PhosphoSiteiQ8BG95.

Proteomic databases

EPDiQ8BG95.
MaxQBiQ8BG95.
PaxDbiQ8BG95.
PRIDEiQ8BG95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045665; ENSMUSP00000047463; ENSMUSG00000073557. [Q8BG95-1]
ENSMUST00000112163; ENSMUSP00000107788; ENSMUSG00000073557. [Q8BG95-2]
GeneIDi329251.

Organism-specific databases

CTDi4660.
MGIiMGI:1916417. Ppp1r12b.

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiQ8BG95.
OrthoDBiEOG7060Q5.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

PROiQ8BG95.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BG95.
ExpressionAtlasiQ8BG95. baseline and differential.
GenevisibleiQ8BG95. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-484 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain, Cerebellum and Pancreas.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; THR-444; SER-502; THR-802; SER-833 AND SER-941, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiMYPT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BG95
Secondary accession number(s): Q8BXY7, Q9D8S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 9, 2007
Last modified: May 11, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.