Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thioredoxin-interacting protein

Gene

Txnip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.By similarity2 Publications

GO - Molecular functioni

  • enzyme inhibitor activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

Keywordsi

Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-interacting protein
Alternative name(s):
Vitamin D3 up-regulated protein 1
Gene namesi
Name:Txnip
Synonyms:Vdup1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1889549 Txnip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Marked reduction in the numbers of natural killer cells, low levels of Il2rb expression in the precursor hematopoietic stem cells and severe lymphoid hyperplasia in the small intestine.1 Publication

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002504901 – 397Thioredoxin-interacting proteinAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63InterchainBy similarity
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei362PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8BG60
PeptideAtlasiQ8BG60
PRIDEiQ8BG60

PTM databases

iPTMnetiQ8BG60
PhosphoSitePlusiQ8BG60

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000038393
CleanExiMM_TXNIP
GenevisibleiQ8BG60 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prmt1Q9JIF02EBI-10689732,EBI-519055

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207912, 1 interactor
IntActiQ8BG60, 1 interactor
STRINGi10090.ENSMUSP00000102710

Structurei

3D structure databases

ProteinModelPortaliQ8BG60
SMRiQ8BG60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arrestin family.Curated

Phylogenomic databases

eggNOGiKOG3780 Eukaryota
ENOG41102NY LUCA
GeneTreeiENSGT00550000074356
HOGENOMiHOG000237328
HOVERGENiHBG066469
InParanoidiQ8BG60
KOiK20910
OMAiISGMCDA
OrthoDBiEOG091G0B0Y
PhylomeDBiQ8BG60
TreeFamiTF313650

Family and domain databases

Gene3Di2.60.40.640, 2 hits
InterProiView protein in InterPro
IPR011021 Arrestin-like_N
IPR014752 Arrestin_C
IPR011022 Arrestin_C-like
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF02752 Arrestin_C, 1 hit
PF00339 Arrestin_N, 1 hit
SMARTiView protein in SMART
SM01017 Arrestin_C, 1 hit
SUPFAMiSSF81296 SSF81296, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG60-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMFKKIKSF EVVFNDPEKV YGSGEKVAGR VIVEVCEVTR VKAVRILACG
60 70 80 90 100
VAKVLWMQGS QQCKQTLDYL RYEDTLLLEE QPTAGENEMV IMRPGNKYEY
110 120 130 140 150
KFGFELPQGP LGTSFKGKYG CVDYWVKAFL DRPSQPTQEA KKNFEVMDLV
160 170 180 190 200
DVNTPDLMAP VSAKKEKKVS CMFIPDGRVS VSARIDRKGF CEGDDISIHA
210 220 230 240 250
DFENTCSRIV VPKAAIVARH TYLANGQTKV FTQKLSSVRG NHIISGTCAS
260 270 280 290 300
WRGKSLRVQK IRPSILGCNI LKVEYSLLIY VSVPGSKKVI LDLPLVIGSR
310 320 330 340 350
SGLSSRTSSM ASRTSSEMSW IDLNIPDTPE APPCYMDIIP EDHRLESPTT
360 370 380 390
PLLDDVDDSQ DSPIFMYAPE FQFMPPPTYT EVDPCVLNNN NNNNNVQ
Length:397
Mass (Da):44,363
Last modified:March 1, 2003 - v1
Checksum:iC160781B207C99A6
GO
Isoform 2 (identifier: Q8BG60-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     84-84: Missing.

Show »
Length:396
Mass (Da):44,292
Checksum:i7F8C2AE2CA5C432A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79E → G in BAB23444 (PubMed:16141072).Curated1
Sequence conflicti165K → E in AAD48499 (PubMed:10843682).Curated1
Sequence conflicti175P → R in AAD48499 (PubMed:10843682).Curated1
Sequence conflicti308S → F in AAG32665 (PubMed:11376942).Curated1
Sequence conflicti308S → F in AAG32666 (PubMed:11376942).Curated1
Sequence conflicti395N → NN in AAH31850 (PubMed:15489334).Curated1
Sequence conflicti395Missing in AAD48499 (PubMed:10843682).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02065384Missing in isoform 2. 4 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173681 mRNA Translation: AAD48499.1
AF282825 Genomic DNA Translation: AAG32665.1
AF282826 mRNA Translation: AAG32666.1
AK004653 mRNA Translation: BAB23444.1
AK082476 mRNA Translation: BAC38504.1
AK088870 mRNA Translation: BAC40625.1
AK089304 mRNA Translation: BAC40834.1
AK089351 mRNA Translation: BAC40850.1
AK089403 mRNA Translation: BAC40868.1
AK155517 mRNA Translation: BAE33304.1
AK171205 mRNA Translation: BAE42311.1
AK171312 mRNA Translation: BAE42386.1
AK171425 mRNA Translation: BAE42444.1
AK171456 mRNA Translation: BAE42465.1
AK171464 mRNA Translation: BAE42470.1
AK171527 mRNA Translation: BAE42508.1
BC031850 mRNA Translation: AAH31850.1
CCDSiCCDS17640.1 [Q8BG60-2]
CCDS38555.1 [Q8BG60-1]
RefSeqiNP_001009935.1, NM_001009935.2 [Q8BG60-1]
NP_076208.2, NM_023719.2 [Q8BG60-2]
UniGeneiMm.410189
Mm.475353

Genome annotation databases

EnsembliENSMUST00000049093; ENSMUSP00000041467; ENSMUSG00000038393 [Q8BG60-2]
ENSMUST00000074519; ENSMUSP00000102710; ENSMUSG00000038393 [Q8BG60-1]
GeneIDi56338
KEGGimmu:56338
UCSCiuc008qnc.2 mouse [Q8BG60-1]
uc008qnd.2 mouse [Q8BG60-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTXNIP_MOUSE
AccessioniPrimary (citable) accession number: Q8BG60
Secondary accession number(s): Q8BGQ0
, Q8K2B2, Q9DC00, Q9EP90, Q9R115
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health