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Protein

Thioredoxin-interacting protein

Gene

Txnip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.By similarity2 Publications

GO - Molecular functioni

  • enzyme inhibitor activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-844456. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-interacting protein
Alternative name(s):
Vitamin D3 up-regulated protein 1
Gene namesi
Name:Txnip
Synonyms:Vdup1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1889549. Txnip.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • mitochondrial intermembrane space Source: Ensembl
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Marked reduction in the numbers of natural killer cells, low levels of Il2rb expression in the precursor hematopoietic stem cells and severe lymphoid hyperplasia in the small intestine.1 Publication

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Thioredoxin-interacting proteinPRO_0000250490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 – 63InterchainBy similarity
Cross-linki213 – 213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei362 – 3621PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BG60.
MaxQBiQ8BG60.
PaxDbiQ8BG60.
PRIDEiQ8BG60.

PTM databases

iPTMnetiQ8BG60.
PhosphoSiteiQ8BG60.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ8BG60.
CleanExiMM_TXNIP.
GenevisibleiQ8BG60. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prmt1Q9JIF02EBI-10689732,EBI-519055

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207912. 1 interaction.
IntActiQ8BG60. 1 interaction.
STRINGi10090.ENSMUSP00000102710.

Structurei

3D structure databases

ProteinModelPortaliQ8BG60.
SMRiQ8BG60. Positions 7-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arrestin family.Curated

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ8BG60.
OMAiLEDQPTG.
OrthoDBiEOG7P02HT.
PhylomeDBiQ8BG60.
TreeFamiTF313650.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG60-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMFKKIKSF EVVFNDPEKV YGSGEKVAGR VIVEVCEVTR VKAVRILACG
60 70 80 90 100
VAKVLWMQGS QQCKQTLDYL RYEDTLLLEE QPTAGENEMV IMRPGNKYEY
110 120 130 140 150
KFGFELPQGP LGTSFKGKYG CVDYWVKAFL DRPSQPTQEA KKNFEVMDLV
160 170 180 190 200
DVNTPDLMAP VSAKKEKKVS CMFIPDGRVS VSARIDRKGF CEGDDISIHA
210 220 230 240 250
DFENTCSRIV VPKAAIVARH TYLANGQTKV FTQKLSSVRG NHIISGTCAS
260 270 280 290 300
WRGKSLRVQK IRPSILGCNI LKVEYSLLIY VSVPGSKKVI LDLPLVIGSR
310 320 330 340 350
SGLSSRTSSM ASRTSSEMSW IDLNIPDTPE APPCYMDIIP EDHRLESPTT
360 370 380 390
PLLDDVDDSQ DSPIFMYAPE FQFMPPPTYT EVDPCVLNNN NNNNNVQ
Length:397
Mass (Da):44,363
Last modified:March 1, 2003 - v1
Checksum:iC160781B207C99A6
GO
Isoform 2 (identifier: Q8BG60-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     84-84: Missing.

Show »
Length:396
Mass (Da):44,292
Checksum:i7F8C2AE2CA5C432A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791E → G in BAB23444 (PubMed:16141072).Curated
Sequence conflicti165 – 1651K → E in AAD48499 (PubMed:10843682).Curated
Sequence conflicti175 – 1751P → R in AAD48499 (PubMed:10843682).Curated
Sequence conflicti308 – 3081S → F in AAG32665 (PubMed:11376942).Curated
Sequence conflicti308 – 3081S → F in AAG32666 (PubMed:11376942).Curated
Sequence conflicti395 – 3951N → NN in AAH31850 (PubMed:15489334).Curated
Sequence conflicti395 – 3951Missing in AAD48499 (PubMed:10843682).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei84 – 841Missing in isoform 2. 4 PublicationsVSP_020653

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173681 mRNA. Translation: AAD48499.1.
AF282825 Genomic DNA. Translation: AAG32665.1.
AF282826 mRNA. Translation: AAG32666.1.
AK004653 mRNA. Translation: BAB23444.1.
AK082476 mRNA. Translation: BAC38504.1.
AK088870 mRNA. Translation: BAC40625.1.
AK089304 mRNA. Translation: BAC40834.1.
AK089351 mRNA. Translation: BAC40850.1.
AK089403 mRNA. Translation: BAC40868.1.
AK155517 mRNA. Translation: BAE33304.1.
AK171205 mRNA. Translation: BAE42311.1.
AK171312 mRNA. Translation: BAE42386.1.
AK171425 mRNA. Translation: BAE42444.1.
AK171456 mRNA. Translation: BAE42465.1.
AK171464 mRNA. Translation: BAE42470.1.
AK171527 mRNA. Translation: BAE42508.1.
BC031850 mRNA. Translation: AAH31850.1.
CCDSiCCDS17640.1. [Q8BG60-2]
CCDS38555.1. [Q8BG60-1]
RefSeqiNP_001009935.1. NM_001009935.2. [Q8BG60-1]
NP_076208.2. NM_023719.2. [Q8BG60-2]
UniGeneiMm.410189.
Mm.475353.

Genome annotation databases

EnsembliENSMUST00000049093; ENSMUSP00000041467; ENSMUSG00000038393. [Q8BG60-2]
ENSMUST00000074519; ENSMUSP00000102710; ENSMUSG00000038393. [Q8BG60-1]
GeneIDi56338.
KEGGimmu:56338.
UCSCiuc008qnc.2. mouse. [Q8BG60-1]
uc008qnd.2. mouse. [Q8BG60-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173681 mRNA. Translation: AAD48499.1.
AF282825 Genomic DNA. Translation: AAG32665.1.
AF282826 mRNA. Translation: AAG32666.1.
AK004653 mRNA. Translation: BAB23444.1.
AK082476 mRNA. Translation: BAC38504.1.
AK088870 mRNA. Translation: BAC40625.1.
AK089304 mRNA. Translation: BAC40834.1.
AK089351 mRNA. Translation: BAC40850.1.
AK089403 mRNA. Translation: BAC40868.1.
AK155517 mRNA. Translation: BAE33304.1.
AK171205 mRNA. Translation: BAE42311.1.
AK171312 mRNA. Translation: BAE42386.1.
AK171425 mRNA. Translation: BAE42444.1.
AK171456 mRNA. Translation: BAE42465.1.
AK171464 mRNA. Translation: BAE42470.1.
AK171527 mRNA. Translation: BAE42508.1.
BC031850 mRNA. Translation: AAH31850.1.
CCDSiCCDS17640.1. [Q8BG60-2]
CCDS38555.1. [Q8BG60-1]
RefSeqiNP_001009935.1. NM_001009935.2. [Q8BG60-1]
NP_076208.2. NM_023719.2. [Q8BG60-2]
UniGeneiMm.410189.
Mm.475353.

3D structure databases

ProteinModelPortaliQ8BG60.
SMRiQ8BG60. Positions 7-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207912. 1 interaction.
IntActiQ8BG60. 1 interaction.
STRINGi10090.ENSMUSP00000102710.

PTM databases

iPTMnetiQ8BG60.
PhosphoSiteiQ8BG60.

Proteomic databases

EPDiQ8BG60.
MaxQBiQ8BG60.
PaxDbiQ8BG60.
PRIDEiQ8BG60.

Protocols and materials databases

DNASUi56338.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049093; ENSMUSP00000041467; ENSMUSG00000038393. [Q8BG60-2]
ENSMUST00000074519; ENSMUSP00000102710; ENSMUSG00000038393. [Q8BG60-1]
GeneIDi56338.
KEGGimmu:56338.
UCSCiuc008qnc.2. mouse. [Q8BG60-1]
uc008qnd.2. mouse. [Q8BG60-2]

Organism-specific databases

CTDi10628.
MGIiMGI:1889549. Txnip.

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ8BG60.
OMAiLEDQPTG.
OrthoDBiEOG7P02HT.
PhylomeDBiQ8BG60.
TreeFamiTF313650.

Enzyme and pathway databases

ReactomeiR-MMU-844456. The NLRP3 inflammasome.

Miscellaneous databases

NextBioi312330.
PROiQ8BG60.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BG60.
CleanExiMM_TXNIP.
GenevisibleiQ8BG60. MM.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vitamin D3 up-regulated protein 1 mediates oxidative stress via suppressing the thioredoxin function."
    Junn E., Han S.H., Im J.Y., Yang Y., Cho E.W., Um H.D., Kim D.K., Lee K.W., Han P.L., Rhee S.G., Choi I.
    J. Immunol. 164:6287-6295(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH TXN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Cloning, genetic characterization, and chromosomal mapping of the mouse VDUP1 gene."
    Ludwig D.L., Kotanides H., Le T., Chavkin D., Bohlen P., Witte L.
    Gene 269:103-112(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Cerebellum, Lung and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary tumor.
  5. "Tumor suppressor VDUP1 increases p27(kip1) stability by inhibiting JAB1."
    Jeon J.H., Lee K.N., Hwang C.Y., Kwon K.S., You K.H., Choi I.
    Cancer Res. 65:4485-4489(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH COPS5.
  6. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTXNIP_MOUSE
AccessioniPrimary (citable) accession number: Q8BG60
Secondary accession number(s): Q8BGQ0
, Q8K2B2, Q9DC00, Q9EP90, Q9R115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.