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Protein

Katanin p80 WD40 repeat-containing subunit B1

Gene

Katnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. The function in regulating microtubule dynamics at spindle poles seems to depend on the association of the katanin KATNA1:KATNB1 complex with ASPM which recruits it to microtubules. Reversely KATNA1:KATNB1 can enhance ASPM blocking activity on microtubule minus-end growth. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p80 WD40 repeat-containing subunit B1UniRule annotation
Short name:
Katanin p80 subunit B1UniRule annotation
Alternative name(s):
p80 kataninUniRule annotation
Gene namesi
Name:Katnb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1921437. Katnb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Homozygous loss of the gene is embryonic lethal. Mutant animals have dramatically reduced body size, reduced limb bud outgrowth, microphthalmia to anophthalmia and forebrain abnormalities ranging from microcephaly to holoprosencephaly. Brains of mutant mice have reduced cycling and proliferating radial neuroepithelial progenitor cells compared to wild-type, with a more profound loss of cells that depend upon asymmetrical cell divisions. These cells also show evidence of increased apoptosis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi538S → L: Disrupts KATNA1:KATNB1 interaction with ASPM. 1 Publication1
Mutagenesisi574Y → A: Disrupts KATNA1:KATNB1 interaction with ASPM; abolishes localization to microtubules minus ends; decreases ASPM localization to microtubules minus ends; no enhancement of ASPM activity in blocking microtubule minus-end growth. 1 Publication1
Mutagenesisi607G → A: Abolishes localization to microtubules. 1 Publication1
Mutagenesisi608V → A: Abolishes localization to microtubules. 1 Publication1
Mutagenesisi609D → A: Abolishes localization to microtubules. 1 Publication1
Mutagenesisi610I → A: Abolishes localization to microtubules. 1 Publication1
Mutagenesisi615R → A: Abolishes localization to microtubules minus ends; decreases ASPM localization to microtubules minus ends; no enhancement of ASPM activity in blocking microtubule minus-end growth. 1 Publication1
Mutagenesisi618K → A: Abolishes localization to microtubules. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510501 – 658Katanin p80 WD40 repeat-containing subunit B1Add BLAST658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BG40.
MaxQBiQ8BG40.
PaxDbiQ8BG40.
PeptideAtlasiQ8BG40.
PRIDEiQ8BG40.

PTM databases

iPTMnetiQ8BG40.
PhosphoSitePlusiQ8BG40.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031787.
CleanExiMM_KATNB1.
ExpressionAtlasiQ8BG40. baseline and differential.
GenevisibleiQ8BG40. MM.

Interactioni

Subunit structurei

Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome (PubMed:16203747). This interaction is weakly competed by KATNBL1 which has a lower affinity for it (By similarity). Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM (PubMed:28436967). Interacts with dynein, microtubules, NDEL1 and PAFAH1B1 (PubMed:16203747). Interacts with KATNAL1; this interaction is weakly competed by KATNBL1 which has a lower affinity for it (By similarity).UniRule annotationBy similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Katna1Q9WV868EBI-7692933,EBI-7692898

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216559. 2 interactors.
IntActiQ8BG40. 3 interactors.
STRINGi10090.ENSMUSP00000034239.

Structurei

Secondary structure

1658
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi487 – 495Combined sources9
Helixi498 – 519Combined sources22
Turni520 – 522Combined sources3
Helixi524 – 534Combined sources11
Helixi537 – 547Combined sources11
Helixi551 – 553Combined sources3
Helixi556 – 569Combined sources14
Helixi575 – 600Combined sources26
Helixi612 – 637Combined sources26
Helixi644 – 653Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LB7X-ray1.50A485-656[»]
A481-658[»]
5NBTX-ray2.40A/C481-658[»]
ProteinModelPortaliQ8BG40.
SMRiQ8BG40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 58WD 1Add BLAST41
Repeati61 – 100WD 2Add BLAST40
Repeati103 – 142WD 3Add BLAST40
Repeati145 – 184WD 4Add BLAST40
Repeati187 – 226WD 5Add BLAST40
Repeati229 – 269WD 6Add BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 300Interaction with centrosomesBy similarityAdd BLAST300
Regioni1 – 284Interaction with dyneinAdd BLAST284
Regioni285 – 437Interaction with PAFAH1B11 PublicationAdd BLAST153
Regioni436 – 658Interaction with KATNA1 and NDEL11 PublicationAdd BLAST223

Sequence similaritiesi

Belongs to the WD repeat KATNB1 family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0267. Eukaryota.
ENOG410XQAC. LUCA.
GeneTreeiENSGT00810000125363.
HOGENOMiHOG000008039.
HOVERGENiHBG052294.
InParanoidiQ8BG40.
KOiK18643.
OMAiWDVQNEN.
OrthoDBiEOG091G0DTV.
PhylomeDBiQ8BG40.
TreeFamiTF332359.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03022. Katanin_p80_B1. 1 hit.
InterProiView protein in InterPro
IPR020472. G-protein_beta_WD-40_rep.
IPR026962. Katanin_bsu1.
IPR028021. Katanin_C-terminal.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF13925. Katanin_con80. 1 hit.
PF00400. WD40. 5 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00320. WD40. 6 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BG40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPVVTKTA WKLQEIVAHA SNVSSLVLGK ASGRLLATGG DDCRVNLWSI
60 70 80 90 100
NKPNCIMSLT GHTSPVESVR LNTPEELIVA GSQSGSIRVW DLEAAKILRT
110 120 130 140 150
LMGHKANICS LDFHPYGEFV ASGSQDTNIK LWDIRRKGCV FRYRGHSQAV
160 170 180 190 200
RCLRFSPDGK WLASAADDHT VKLWDLTAGK MMSEFPGHTG PVNVVEFHPN
210 220 230 240 250
EYLLASGSSD RTIRFWDLEK FQVVSCIEGE PGPVRSVLFN PDGCCLYSGC
260 270 280 290 300
QDSLRVYGWE PERCFDVVLV NWGKVADLAI CNDQLIGVAF SQSNVSSYVV
310 320 330 340 350
DLTRVTRTGT VTQDPVQANQ PLTQQTPNPG VSLRRIYERP STTCSKPQRV
360 370 380 390 400
KHNSESERRS PSSEDDRDER ESRAEIQNAE DYNEIFQPKN SISRTPPRRS
410 420 430 440 450
EPFPAPPEDD AATVKEVSKP SPAMDVQLPQ LPVPNLEVPA RPSVMTSTPA
460 470 480 490 500
PKGEPDIIPA TRNEPIGLKA SDFLPAVKVP QQAELVDEDA MSQIRKGHDT
510 520 530 540 550
MFVVLTSRHK NLDTVRAVWT TGDIKTSVDS AVAINDLSVV VDLLNIVNQK
560 570 580 590 600
ASLWKLDLCT TVLPQIEKLL QSKYESYVQT GCTSLKLILQ RFLPLITDIL
610 620 630 640 650
AAPPSVGVDI SREERLHKCR LCFKQLKSIS GLVKSKSGLS GRHGSAFREL

HLLMASLD
Length:658
Mass (Da):72,639
Last modified:March 1, 2003 - v1
Checksum:iCB04B82DA34A19EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti455P → H in BAC27487 (PubMed:16141072).Curated1
Sequence conflicti596Missing in BAB26884 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010364 mRNA. Translation: BAB26884.1.
AK031630 mRNA. Translation: BAC27487.1.
AK034103 mRNA. Translation: BAC28588.1.
AK049345 mRNA. Translation: BAC33697.1.
AK087973 mRNA. Translation: BAC40067.1.
BC024500 mRNA. Translation: AAH24500.1.
BC045200 mRNA. Translation: AAH45200.1.
CCDSiCCDS22555.1.
RefSeqiNP_083081.2. NM_028805.2.
XP_006531473.1. XM_006531410.3.
XP_006531474.1. XM_006531411.3.
XP_006531475.1. XM_006531412.1.
UniGeneiMm.28382.

Genome annotation databases

EnsembliENSMUST00000034239; ENSMUSP00000034239; ENSMUSG00000031787.
GeneIDi74187.
KEGGimmu:74187.
UCSCiuc009mxt.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiKTNB1_MOUSE
AccessioniPrimary (citable) accession number: Q8BG40
Secondary accession number(s): Q8CD18, Q8R1J0, Q9CWV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: September 27, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families