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Protein

RUN and SH3 domain-containing protein 1

Gene

Rusc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
RUN and SH3 domain-containing protein 1
Gene namesi
Name:Rusc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1919546. Rusc1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus By similarity
  • Cytoplasmcytoskeleton 1 Publication
  • Cytoplasmic vesicle 1 Publication
  • Early endosome 1 Publication
  • Cell junctionsynapsepostsynaptic cell membranepostsynaptic density 1 Publication
  • Golgi apparatus 1 Publication

  • Note: Translocated to the nuclear envelope upon stimulation with NGF (By similarity). Associated with membranes and microtubules.By similarity

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: UniProtKB
  • early endosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Microtubule, Nucleus, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893RUN and SH3 domain-containing protein 1PRO_0000097533Add
BLAST

Post-translational modificationi

Phosphorylated on serine residues following nuclear translocation.By similarity
Polyubiquitinated; polyubiquitination involves TRAF6.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BG26.
MaxQBiQ8BG26.
PaxDbiQ8BG26.
PRIDEiQ8BG26.

PTM databases

iPTMnetiQ8BG26.
PhosphoSiteiQ8BG26.

Expressioni

Tissue specificityi

Expressed in brain, brain stem and spinal chord (at protein level).

Developmental stagei

Expressed at E15 in hippocampal, cortical and cerebellar brain, and brain stem and spinal chord. At E18, expression strongly overlaps with TUBB3 expression in post-mitotic neurons throughout the entire brain. Expression levels increase to E18/P1 after which the levels decline in the hippocampus, cerebellum and brain stem and spinal chord into adulthood while remaining high in the cortex.1 Publication

Gene expression databases

BgeeiQ8BG26.
CleanExiMM_RUSC1.
ExpressionAtlasiQ8BG26. baseline and differential.
GenevisibleiQ8BG26. MM.

Interactioni

Subunit structurei

Interacts with IKBKG and TRAF6 (By similarity). Interacts with F-actin, acetylated actin, TUBB3, STX1A, KIF5B and KLC1.By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8BG26. 5 interactions.
STRINGi10090.ENSMUSP00000088447.

Structurei

3D structure databases

ProteinModelPortaliQ8BG26.
SMRiQ8BG26. Positions 476-659, 833-890.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini515 – 659145RUNPROSITE-ProRule annotationAdd
BLAST
Domaini835 – 89359SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni463 – 598136Interaction with TRAF6By similarityAdd
BLAST
Regioni599 – 66567Interaction with IKBKGBy similarityAdd
BLAST

Domaini

The RUN domain is necessary for NGF induced nuclear redistribution.By similarity

Sequence similaritiesi

Contains 1 RUN domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG0711. Eukaryota.
COG0142. LUCA.
GeneTreeiENSGT00840000129820.
HOGENOMiHOG000154229.
HOVERGENiHBG058522.
InParanoidiQ8BG26.
OMAiWKTNTGI.
OrthoDBiEOG757CWF.
PhylomeDBiQ8BG26.
TreeFamiTF332235.

Family and domain databases

InterProiIPR004012. Run_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50826. RUN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG26-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSPQRALLC NLNHIHLQHV SLGLHLSRRP ELREGPLSTP PPPGDTGGKE
60 70 80 90 100
SRGPCSGTLV DANSNSPAVP CRCCQEHGSS IENQQDPSQE EEAVSPSDPG
110 120 130 140 150
CSSSLSSCSD LSPDESPVSV YSRDLPGNED ANPQPSTLEL GSPLAPAGPS
160 170 180 190 200
TCSPDSFCCS PDSCSGISSP PGPDLDSNCN ALTTCQDLPS PGLEEEEDSG
210 220 230 240 250
EQDLATSELS ETEDGRIDAG KAEPSWKINP IWKIDTEKTE AGWKTIEDSD
260 270 280 290 300
SGRKTDENTN SSLKTESGKL ASCLNTNSGS KIDAGKTDGG WRGDVSQEPV
310 320 330 340 350
PHRTITSFHE LAQKRKRGPG LPLVPQAKKD RSDWLIVFSP DTELPPTGSL
360 370 380 390 400
GGSLAPPREV TTFKELRSRS RAQPPPVPPR DPPAGWALVP PRPPPPPVPP
410 420 430 440 450
RRKKNRLGLQ PIAEGLSEEG RAASPRAGEE ASASQEPEEP RAHAVVRSSW
460 470 480 490 500
SFAGVPGAQR LWMAEAQSGT GQLQEQKKGL LIAVSASVDK IISHFGAARN
510 520 530 540 550
LVQKAQLGDS RLSPDVGHLV LTTLCPALHA LVADGLKPFR KDLITGQRRS
560 570 580 590 600
SPWSVVEASV KPGSCTHSMG SLYSQVSRLA PLSSSRSRFH AFILGLLNTK
610 620 630 640 650
QLELWFSSLQ EDAGLLSLLY LPTGFFSLAR GSCPSLATEL LLLLQPLSVL
660 670 680 690 700
TFHLDLLFEH HHHLPVGLQQ APAPSCPPPA LQQTMQAVLH WGERLAQSLR
710 720 730 740 750
GTSGESTTDS STPSARPPAG SWWDQLTQAS RVYASGGTEG FPLLRWGPRR
760 770 780 790 800
HGTTAEAAQE APPPTEQTTP GRSVWLGRLF GVPGCPSETE SGAFKSRRPS
810 820 830 840 850
SWLPPTVSVL ALVKRGTPPE TPPEALVSSP GSVVQADRAV RALCDHTAAG
860 870 880 890
PDQLSFQRGE LLRVIATVDE DWLRCGRDGV EGLVPVGYTS LVL
Length:893
Mass (Da):95,195
Last modified:July 19, 2004 - v2
Checksum:iB7CB4102A3CFAAB2
GO
Isoform 2 (identifier: Q8BG26-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-462: Missing.

Show »
Length:431
Mass (Da):46,158
Checksum:iCFA15969F67C3FDC
GO
Isoform 3 (identifier: Q8BG26-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     445-446: VV → VAGPSLFPRP...RRPPPPPRQL

Note: No experimental confirmation available.
Show »
Length:1,030
Mass (Da):109,515
Checksum:i358BB46D40340220
GO

Sequence cautioni

The sequence BAC27820.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC30411.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC31333.1 differs from that shown. Reason: Erroneous termination at position 462. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti345 – 3451P → Q in BAC27820 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 462462Missing in isoform 2. 1 PublicationVSP_010856Add
BLAST
Alternative sequencei445 – 4462VV → VAGPSLFPRPPVFRFSADGR PLLEGGGAGAPGSLLFTPLT GWSNSRLRLLGAASPPEEQL LPVRLSPVGAYSPPTRGALP CLASPELALLLSPLFPRSST FPAAAPLPRQVPAPPLPTPP CPPTAPRWTRRPPPPPRQL in isoform 3. 1 PublicationVSP_010857

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032334 mRNA. Translation: BAC27820.1. Different initiation.
AK039664 mRNA. Translation: BAC30411.1. Different initiation.
AK042689 mRNA. Translation: BAC31333.1. Sequence problems.
BC056360 mRNA. Translation: AAH56360.1.
BC057034 mRNA. Translation: AAH57034.1.
CCDSiCCDS38485.1. [Q8BG26-1]
CCDS38486.1. [Q8BG26-3]
CCDS50956.1. [Q8BG26-2]
RefSeqiNP_001077276.1. NM_001083807.1. [Q8BG26-1]
NP_001077277.1. NM_001083808.1. [Q8BG26-2]
NP_082464.2. NM_028188.2. [Q8BG26-3]
XP_006502197.1. XM_006502134.2. [Q8BG26-2]
UniGeneiMm.27687.

Genome annotation databases

EnsembliENSMUST00000052539; ENSMUSP00000056640; ENSMUSG00000041263. [Q8BG26-1]
ENSMUST00000090929; ENSMUSP00000088447; ENSMUSG00000041263. [Q8BG26-3]
ENSMUST00000166687; ENSMUSP00000130477; ENSMUSG00000041263. [Q8BG26-2]
GeneIDi72296.
KEGGimmu:72296.
UCSCiuc008pxm.1. mouse. [Q8BG26-3]
uc008pxn.1. mouse. [Q8BG26-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032334 mRNA. Translation: BAC27820.1. Different initiation.
AK039664 mRNA. Translation: BAC30411.1. Different initiation.
AK042689 mRNA. Translation: BAC31333.1. Sequence problems.
BC056360 mRNA. Translation: AAH56360.1.
BC057034 mRNA. Translation: AAH57034.1.
CCDSiCCDS38485.1. [Q8BG26-1]
CCDS38486.1. [Q8BG26-3]
CCDS50956.1. [Q8BG26-2]
RefSeqiNP_001077276.1. NM_001083807.1. [Q8BG26-1]
NP_001077277.1. NM_001083808.1. [Q8BG26-2]
NP_082464.2. NM_028188.2. [Q8BG26-3]
XP_006502197.1. XM_006502134.2. [Q8BG26-2]
UniGeneiMm.27687.

3D structure databases

ProteinModelPortaliQ8BG26.
SMRiQ8BG26. Positions 476-659, 833-890.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BG26. 5 interactions.
STRINGi10090.ENSMUSP00000088447.

PTM databases

iPTMnetiQ8BG26.
PhosphoSiteiQ8BG26.

Proteomic databases

EPDiQ8BG26.
MaxQBiQ8BG26.
PaxDbiQ8BG26.
PRIDEiQ8BG26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052539; ENSMUSP00000056640; ENSMUSG00000041263. [Q8BG26-1]
ENSMUST00000090929; ENSMUSP00000088447; ENSMUSG00000041263. [Q8BG26-3]
ENSMUST00000166687; ENSMUSP00000130477; ENSMUSG00000041263. [Q8BG26-2]
GeneIDi72296.
KEGGimmu:72296.
UCSCiuc008pxm.1. mouse. [Q8BG26-3]
uc008pxn.1. mouse. [Q8BG26-1]

Organism-specific databases

CTDi23623.
MGIiMGI:1919546. Rusc1.

Phylogenomic databases

eggNOGiKOG0711. Eukaryota.
COG0142. LUCA.
GeneTreeiENSGT00840000129820.
HOGENOMiHOG000154229.
HOVERGENiHBG058522.
InParanoidiQ8BG26.
OMAiWKTNTGI.
OrthoDBiEOG757CWF.
PhylomeDBiQ8BG26.
TreeFamiTF332235.

Miscellaneous databases

ChiTaRSiRusc1. mouse.
NextBioi335934.
PROiQ8BG26.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BG26.
CleanExiMM_RUSC1.
ExpressionAtlasiQ8BG26. baseline and differential.
GenevisibleiQ8BG26. MM.

Family and domain databases

InterProiIPR004012. Run_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50826. RUN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Olfactory bulb and Spinal cord.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "Nesca, a novel neuronal adapter protein, links the molecular motor kinesin with the pre-synaptic membrane protein, syntaxin-1, in hippocampal neurons."
    Macdonald J.I., Dietrich A., Gamble S., Hryciw T., Grant R.I., Meakin S.O.
    J. Neurochem. 121:861-880(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ACTIN, TUBB3; STX1A; KIF5B AND KLC1 TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiRUSC1_MOUSE
AccessioniPrimary (citable) accession number: Q8BG26
Secondary accession number(s): Q6PHT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: May 11, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.