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Q8BG22 (CLCA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-activated chloride channel regulator 2
Alternative name(s):
Calcium-activated chloride channel family member 5
Short name=mCLCA5
Gene names
Name:Clca2
Synonyms:Clca5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length942 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner, and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4. Ref.1 Ref.2

Subcellular location

Cell membrane; Single-pass type I membrane protein. Basal cell membrane; Single-pass type I membrane protein. Cell junction Ref.2.

Tissue specificity

Highly expressed in eye, spleen, lung, kidney, uterus, and endothelial cells. Weakly expressed in heart and throughout the gastrointestinal tract. Highly expressed in mammary cell lines. Its expression in immortalized cell line HC11 correlates with slow or arrested growth. Re-expression in mammary tumor cells reduces colony survival. Ref.1 Ref.2

Induction

By 30-fold when cells are deprived of growth factors or anchorage in mammary epithelial cell. Down-regulated in metastatic mammary tumor cell lines. Ref.1

Post-translational modification

The 155 kDa mature form is processed producing a 65 kDa form.

N-glycosylated By similarity.

Sequence similarities

Belongs to the CLCR family.

Contains 1 VWFA domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BG22-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BG22-2)

The sequence of this isoform differs from the canonical sequence as follows:
     675-942: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 942910Calcium-activated chloride channel regulator 2
PRO_0000333696

Regions

Topological domain33 – 905873Extracellular Potential
Transmembrane906 – 92621Helical; Potential
Topological domain927 – 94216Cytoplasmic Potential
Domain311 – 483173VWFA

Amino acid modifications

Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2351N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Glycosylation5801N-linked (GlcNAc...) Potential
Glycosylation6371N-linked (GlcNAc...) Potential
Glycosylation8211N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence675 – 942268Missing in isoform 2.
VSP_033515

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D0511E33CF317E4F

FASTA942103,626
        10         20         30         40         50         60 
MTHRDSTGPV IGLKLVTLLF TLSPELLFLG AGLKLKENGY DGLLVAINPR VPEDLKLITN 

        70         80         90        100        110        120 
IKEMITEASF YLFNATKRRV FFRNVQILVP ATWTDHNYSR VRQESYDKAN VIVAEQSEEH 

       130        140        150        160        170        180 
GDDPYTLQHR GCGQEGRYIH FTPSFLLNDE LAAGYGARGR VFVHEWAHLR WGVFDEYNND 

       190        200        210        220        230        240 
KPFYVNGRNE IQVTRCSSDI TGVFVCEKGL CPHEDCIISK IFREGCTFLY NSTQNATGSI 

       250        260        270        280        290        300 
MFMPSLPSVV EFCNESTHNQ EAPNLQNQVC SLRSTWDVIT ASSDLNHSLP VHGVGLPAPP 

       310        320        330        340        350        360 
TFSLLQAGDR VVCLVIDVSR KMAEGDRLLR LQQAAELYLM QVVEAHTFVG IVTFDSKGEI 

       370        380        390        400        410        420 
RASLQQIYSD DDRKLLVSYL PTAVSTDAET NICAGVKKGF EVVEERNGRA DGSVLILVTS 

       430        440        450        460        470        480 
GADEHIANCL LTSMNSGSTI HSMALGSSAA RKVGELSRLT GGLKFFIPDK FTSNGMTEAF 

       490        500        510        520        530        540 
VRISSGTGDI FQQSLQVESV CETVQPQHQL ADTMTVDSAV GNDTLFLVTW QTGGPPEIAL 

       550        560        570        580        590        600 
LDPSGRKYNT GDFIINLAFR TASLKIPGTA KHGHWTYTLN NTHHSPQALK VTVASRASSL 

       610        620        630        640        650        660 
AMSPATLEAF VERDSTYFPQ PVIIYANVRK GLHPILNATV VATVEPEAGD PVVLQLLDGG 

       670        680        690        700        710        720 
AGADVIRNDG IYSRYFSSFA VSGSYSLTVH VRHSPSTSTL ALPVPGNHAM YVPGYITNDN 

       730        740        750        760        770        780 
IQMNAPKNLG HRPVKERWGF SRVSSGGSFS VLGVPDGPHP DMFPPCKITD LEAMKVEDDV 

       790        800        810        820        830        840 
VLSWTAPGED FDQGQTTSYE IRMSRSLWNI RDDFDNAILV NSSELVPQHA GTRETFTFSP 

       850        860        870        880        890        900 
KLVTHELDHE LAEDAQEPYI VYVALRAMDR SSLRSAVSNI ALVSMSLPPN SSPVVSRDDL 

       910        920        930        940 
ILKGVLTTVG LIAILCLIMV VAHCIFNRKK RPSRKENETK FL 

« Hide

Isoform 2 [UniParc].

Checksum: 190E0E58E4C6D0F1
Show »

FASTA67473,983

References

« Hide 'large scale' references
[1]"Re-expression of detachment-inducible chloride channel mCLCA5 suppresses growth of metastatic breast cancer cells."
Beckley J.R., Pauli B.U., Elble R.C.
J. Biol. Chem. 279:41634-41641(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: BALB/c.
[2]"Molecular and functional analyses of two new calcium-activated chloride channel family members from mouse eye and intestine."
Evans S.R., Thoreson W.B., Beck C.L.
J. Biol. Chem. 279:41792-41800(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Eye.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Head and Skin.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: 129.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY161007 mRNA. Translation: AAO18366.1.
AK028704 mRNA. Translation: BAC26076.1.
AK035512 mRNA. Translation: BAC29086.1.
AK048276 mRNA. Translation: BAC33291.1.
BC096379 mRNA. Translation: AAH96379.1.
CCDSCCDS17890.1. [Q8BG22-1]
RefSeqNP_848812.1. NM_178697.4. [Q8BG22-1]
XP_006501498.1. XM_006501435.1. [Q8BG22-2]
UniGeneMm.331420.

3D structure databases

ProteinModelPortalQ8BG22.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000036029.

Protein family/group databases

MEROPSM87.003.

PTM databases

PhosphoSiteQ8BG22.

Proteomic databases

PaxDbQ8BG22.
PRIDEQ8BG22.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040465; ENSMUSP00000036029; ENSMUSG00000036960. [Q8BG22-1]
GeneID229933.
KEGGmmu:229933.
UCSCuc008rqg.1. mouse. [Q8BG22-1]

Organism-specific databases

CTD229933.
MGIMGI:2139758. Clca5.

Phylogenomic databases

eggNOGNOG42884.
GeneTreeENSGT00390000004810.
HOGENOMHOG000015107.
HOVERGENHBG005560.
InParanoidQ8BG22.
KOK05028.
OMAEKGPCPQ.
OrthoDBEOG78WKR1.
PhylomeDBQ8BG22.
TreeFamTF328396.

Gene expression databases

BgeeQ8BG22.
GenevestigatorQ8BG22.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004727. CaCC_prot.
IPR013642. Cl_channel_Ca.
IPR015394. DUF1973.
IPR002035. VWF_A.
[Graphical view]
PfamPF08434. CLCA_N. 1 hit.
PF09315. DUF1973. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
TIGRFAMsTIGR00868. hCaCC. 1 hit.
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio379743.
PROQ8BG22.
SOURCESearch...

Entry information

Entry nameCLCA2_MOUSE
AccessionPrimary (citable) accession number: Q8BG22
Secondary accession number(s): Q8BZF7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot