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Q8BG22

- CLCA2_MOUSE

UniProt

Q8BG22 - CLCA2_MOUSE

Protein

Calcium-activated chloride channel regulator 2

Gene

Clca2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner, and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4.2 Publications

    GO - Molecular functioni

    1. chloride channel activity Source: MGI
    2. ligand-gated ion channel activity Source: MGI

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. chloride transmembrane transport Source: GOC
    3. chloride transport Source: MGI
    4. ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Cell adhesion, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Chloride

    Protein family/group databases

    MEROPSiM87.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-activated chloride channel regulator 2
    Alternative name(s):
    Calcium-activated chloride channel family member 5
    Short name:
    mCLCA5
    Gene namesi
    Name:Clca2
    Synonyms:Clca5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:2139758. Clca5.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Basal cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell junction 1 Publication

    GO - Cellular componenti

    1. basal plasma membrane Source: UniProtKB-SubCell
    2. cell junction Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 942910Calcium-activated chloride channel regulator 2PRO_0000333696Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi580 – 5801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi637 – 6371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi821 – 8211N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The 155 kDa mature form is processed producing a 65 kDa form.
    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8BG22.
    PRIDEiQ8BG22.

    PTM databases

    PhosphoSiteiQ8BG22.

    Expressioni

    Tissue specificityi

    Highly expressed in eye, spleen, lung, kidney, uterus, and endothelial cells. Weakly expressed in heart and throughout the gastrointestinal tract. Highly expressed in mammary cell lines. Its expression in immortalized cell line HC11 correlates with slow or arrested growth. Re-expression in mammary tumor cells reduces colony survival.2 Publications

    Inductioni

    By 30-fold when cells are deprived of growth factors or anchorage in mammary epithelial cell. Down-regulated in metastatic mammary tumor cell lines.1 Publication

    Gene expression databases

    BgeeiQ8BG22.
    GenevestigatoriQ8BG22.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000036029.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BG22.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 905873ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini927 – 94216CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei906 – 92621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini311 – 483173VWFAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CLCR family.Curated
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42884.
    GeneTreeiENSGT00390000004810.
    HOGENOMiHOG000015107.
    HOVERGENiHBG005560.
    InParanoidiQ8BG22.
    KOiK05028.
    OMAiEKGPCPQ.
    OrthoDBiEOG78WKR1.
    PhylomeDBiQ8BG22.
    TreeFamiTF328396.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR004727. CaCC_prot.
    IPR013642. Cl_channel_Ca.
    IPR015394. DUF1973.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF08434. CLCA_N. 1 hit.
    PF09315. DUF1973. 1 hit.
    [Graphical view]
    SMARTiSM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    TIGRFAMsiTIGR00868. hCaCC. 1 hit.
    PROSITEiPS50234. VWFA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BG22-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTHRDSTGPV IGLKLVTLLF TLSPELLFLG AGLKLKENGY DGLLVAINPR    50
    VPEDLKLITN IKEMITEASF YLFNATKRRV FFRNVQILVP ATWTDHNYSR 100
    VRQESYDKAN VIVAEQSEEH GDDPYTLQHR GCGQEGRYIH FTPSFLLNDE 150
    LAAGYGARGR VFVHEWAHLR WGVFDEYNND KPFYVNGRNE IQVTRCSSDI 200
    TGVFVCEKGL CPHEDCIISK IFREGCTFLY NSTQNATGSI MFMPSLPSVV 250
    EFCNESTHNQ EAPNLQNQVC SLRSTWDVIT ASSDLNHSLP VHGVGLPAPP 300
    TFSLLQAGDR VVCLVIDVSR KMAEGDRLLR LQQAAELYLM QVVEAHTFVG 350
    IVTFDSKGEI RASLQQIYSD DDRKLLVSYL PTAVSTDAET NICAGVKKGF 400
    EVVEERNGRA DGSVLILVTS GADEHIANCL LTSMNSGSTI HSMALGSSAA 450
    RKVGELSRLT GGLKFFIPDK FTSNGMTEAF VRISSGTGDI FQQSLQVESV 500
    CETVQPQHQL ADTMTVDSAV GNDTLFLVTW QTGGPPEIAL LDPSGRKYNT 550
    GDFIINLAFR TASLKIPGTA KHGHWTYTLN NTHHSPQALK VTVASRASSL 600
    AMSPATLEAF VERDSTYFPQ PVIIYANVRK GLHPILNATV VATVEPEAGD 650
    PVVLQLLDGG AGADVIRNDG IYSRYFSSFA VSGSYSLTVH VRHSPSTSTL 700
    ALPVPGNHAM YVPGYITNDN IQMNAPKNLG HRPVKERWGF SRVSSGGSFS 750
    VLGVPDGPHP DMFPPCKITD LEAMKVEDDV VLSWTAPGED FDQGQTTSYE 800
    IRMSRSLWNI RDDFDNAILV NSSELVPQHA GTRETFTFSP KLVTHELDHE 850
    LAEDAQEPYI VYVALRAMDR SSLRSAVSNI ALVSMSLPPN SSPVVSRDDL 900
    ILKGVLTTVG LIAILCLIMV VAHCIFNRKK RPSRKENETK FL 942
    Length:942
    Mass (Da):103,626
    Last modified:March 1, 2003 - v1
    Checksum:iD0511E33CF317E4F
    GO
    Isoform 2 (identifier: Q8BG22-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         675-942: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:674
    Mass (Da):73,983
    Checksum:i190E0E58E4C6D0F1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei675 – 942268Missing in isoform 2. 1 PublicationVSP_033515Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY161007 mRNA. Translation: AAO18366.1.
    AK028704 mRNA. Translation: BAC26076.1.
    AK035512 mRNA. Translation: BAC29086.1.
    AK048276 mRNA. Translation: BAC33291.1.
    BC096379 mRNA. Translation: AAH96379.1.
    CCDSiCCDS17890.1. [Q8BG22-1]
    RefSeqiNP_848812.1. NM_178697.4. [Q8BG22-1]
    XP_006501498.1. XM_006501435.1. [Q8BG22-2]
    UniGeneiMm.331420.

    Genome annotation databases

    EnsembliENSMUST00000040465; ENSMUSP00000036029; ENSMUSG00000036960. [Q8BG22-1]
    GeneIDi229933.
    KEGGimmu:229933.
    UCSCiuc008rqg.1. mouse. [Q8BG22-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY161007 mRNA. Translation: AAO18366.1 .
    AK028704 mRNA. Translation: BAC26076.1 .
    AK035512 mRNA. Translation: BAC29086.1 .
    AK048276 mRNA. Translation: BAC33291.1 .
    BC096379 mRNA. Translation: AAH96379.1 .
    CCDSi CCDS17890.1. [Q8BG22-1 ]
    RefSeqi NP_848812.1. NM_178697.4. [Q8BG22-1 ]
    XP_006501498.1. XM_006501435.1. [Q8BG22-2 ]
    UniGenei Mm.331420.

    3D structure databases

    ProteinModelPortali Q8BG22.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000036029.

    Protein family/group databases

    MEROPSi M87.003.

    PTM databases

    PhosphoSitei Q8BG22.

    Proteomic databases

    PaxDbi Q8BG22.
    PRIDEi Q8BG22.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040465 ; ENSMUSP00000036029 ; ENSMUSG00000036960 . [Q8BG22-1 ]
    GeneIDi 229933.
    KEGGi mmu:229933.
    UCSCi uc008rqg.1. mouse. [Q8BG22-1 ]

    Organism-specific databases

    CTDi 229933.
    MGIi MGI:2139758. Clca5.

    Phylogenomic databases

    eggNOGi NOG42884.
    GeneTreei ENSGT00390000004810.
    HOGENOMi HOG000015107.
    HOVERGENi HBG005560.
    InParanoidi Q8BG22.
    KOi K05028.
    OMAi EKGPCPQ.
    OrthoDBi EOG78WKR1.
    PhylomeDBi Q8BG22.
    TreeFami TF328396.

    Miscellaneous databases

    NextBioi 379743.
    PROi Q8BG22.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BG22.
    Genevestigatori Q8BG22.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR004727. CaCC_prot.
    IPR013642. Cl_channel_Ca.
    IPR015394. DUF1973.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF08434. CLCA_N. 1 hit.
    PF09315. DUF1973. 1 hit.
    [Graphical view ]
    SMARTi SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    TIGRFAMsi TIGR00868. hCaCC. 1 hit.
    PROSITEi PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Re-expression of detachment-inducible chloride channel mCLCA5 suppresses growth of metastatic breast cancer cells."
      Beckley J.R., Pauli B.U., Elble R.C.
      J. Biol. Chem. 279:41634-41641(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: BALB/c.
    2. "Molecular and functional analyses of two new calcium-activated chloride channel family members from mouse eye and intestine."
      Evans S.R., Thoreson W.B., Beck C.L.
      J. Biol. Chem. 279:41792-41800(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Eye.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Head and Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: 129.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiCLCA2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BG22
    Secondary accession number(s): Q8BZF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3