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Protein

Sodium-dependent neutral amino acid transporter B(0)AT2

Gene

Slc6a15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent (By similarity).By similarity

Kineticsi

  1. KM=195 µM for proline (in the presence of 100 mM NaCL)1 Publication
  2. KM=510 µM for proline (in the presence of 30 mM NaCL)1 Publication
  3. KM=790 µM for proline (in the presence of 3 mM NaCL)1 Publication
  4. KM=81 µM for leucine1 Publication
  5. KM=58 µM for isoleucine1 Publication
  6. KM=40 µM for methionine1 Publication
  7. KM=670 µM for alanine1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • leucine transport Source: UniProtKB
    • neutral amino acid transport Source: UniProtKB
    • proline transmembrane transport Source: GOC
    • proline transport Source: UniProtKB
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent neutral amino acid transporter B(0)AT2
    Alternative name(s):
    Sodium- and chloride-dependent neurotransmitter transporter NTT73
    Solute carrier family 6 member 15
    Transporter v7-3
    Gene namesi
    Name:Slc6a15
    Synonyms:B0at2, Ntt73
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 10

    Organism-specific databases

    MGIiMGI:2143484. Slc6a15.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6969CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei70 – 9021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei98 – 11720Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Topological domaini163 – 22563ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei226 – 24419Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei253 – 27018Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei306 – 32318Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35622Helical; Name=7Sequence AnalysisAdd
    BLAST
    Topological domaini357 – 45296ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei453 – 47220Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei496 – 51419Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei530 – 55021Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei571 – 59222Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei620 – 64223Helical; Name=12Sequence AnalysisAdd
    BLAST
    Topological domaini643 – 72987CytoplasmicSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and fertile and fail to demonstrate any striking abnormality in motor and sensory functions. Other transporters could help to compensate for lost of SLC6A15.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Sodium-dependent neutral amino acid transporter B(0)AT2PRO_0000214799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551PhosphoserineBy similarity
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
    Modified residuei687 – 6871PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BG16.
    PaxDbiQ8BG16.
    PRIDEiQ8BG16.

    PTM databases

    PhosphoSiteiQ8BG16.

    Expressioni

    Tissue specificityi

    Significant expressed in brain, lung and kidney. regions, the cortex, the cerebellum and the brain stem.1 Publication

    Developmental stagei

    Detected throughout development, starting with the pre-implantation embryo.1 Publication

    Gene expression databases

    BgeeiQ8BG16.
    ExpressionAtlasiQ8BG16. baseline and differential.
    GenevisibleiQ8BG16. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000073829.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BG16.
    SMRiQ8BG16. Positions 59-640.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00760000119044.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiQ8BG16.
    KOiK05048.
    OMAiAWVVVCL.
    OrthoDBiEOG793B71.
    PhylomeDBiQ8BG16.
    TreeFamiTF352709.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BG16-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EEVSKKSELI VDVQEEKDTD
    60 70 80 90 100
    AEDGSEADDE RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL
    110 120 130 140 150
    LPYLILLLVI GIPLFFLELS VGQRIRRGSI GVWNYISPKL GGIGFASCVV
    160 170 180 190 200
    CYFVALYYNV IIGWTLFYFS QSFQQPLPWD QCPLVKNASH TYVEPECEQS
    210 220 230 240 250
    SATTYYWYRE ALDITSSISD SGGLNWKMTV CLLVAWVMVC LAMIKGIQSS
    260 270 280 290 300
    GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR
    310 320 330 340 350
    EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL
    360 370 380 390 400
    VVFAVLGFKA NIVNEKCISQ NSEMILKLLK MGNISWDVIP HHINLSAVTV
    410 420 430 440 450
    EDYRLVYDII QKVKEEEFAV LHLNACQIED ELNKAVQGTG LAFIAFTEAM
    460 470 480 490 500
    THFPASPFWS VMFFLMLINL GLGSMFGTIE GIITPIVDTF KVRKEILTVI
    510 520 530 540 550
    CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE NIAVSFVYGI
    560 570 580 590 600
    DKFIEDLTDM LGFAPSKYYY YMWKYISPLM LLTLLIASIV NMGLSPPGYN
    610 620 630 640 650
    AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFIIR RCNLIDDSSG
    660 670 680 690 700
    NLASVTYKRG RVLKEPVNLE GDDASLIHGK IPSEMSSPNF GKNIYRKQSG
    710 720
    SPTLDTAPNG RYGIGYLMAD MPDMPESDL
    Length:729
    Mass (Da):81,792
    Last modified:March 1, 2003 - v1
    Checksum:iD336735E9D1E9B97
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY149280 mRNA. Translation: AAN75437.1.
    AY149281 mRNA. Translation: AAN75438.1.
    AY149282 Genomic DNA. Translation: AAN75439.1.
    AM085111 mRNA. Translation: CAJ29896.1.
    AK036136 mRNA. Translation: BAC29315.1.
    CH466539 Genomic DNA. Translation: EDL21676.1.
    CH466539 Genomic DNA. Translation: EDL21677.1.
    BC076593 mRNA. Translation: AAH76593.1.
    CCDSiCCDS24156.1.
    RefSeqiNP_001239259.1. NM_001252330.1.
    NP_780537.1. NM_175328.3.
    XP_006513045.1. XM_006512982.2.
    XP_006513046.1. XM_006512983.2.
    XP_006513047.1. XM_006512984.2.
    UniGeneiMm.458408.

    Genome annotation databases

    EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
    ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
    GeneIDi103098.
    KEGGimmu:103098.
    UCSCiuc007gyj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY149280 mRNA. Translation: AAN75437.1.
    AY149281 mRNA. Translation: AAN75438.1.
    AY149282 Genomic DNA. Translation: AAN75439.1.
    AM085111 mRNA. Translation: CAJ29896.1.
    AK036136 mRNA. Translation: BAC29315.1.
    CH466539 Genomic DNA. Translation: EDL21676.1.
    CH466539 Genomic DNA. Translation: EDL21677.1.
    BC076593 mRNA. Translation: AAH76593.1.
    CCDSiCCDS24156.1.
    RefSeqiNP_001239259.1. NM_001252330.1.
    NP_780537.1. NM_175328.3.
    XP_006513045.1. XM_006512982.2.
    XP_006513046.1. XM_006512983.2.
    XP_006513047.1. XM_006512984.2.
    UniGeneiMm.458408.

    3D structure databases

    ProteinModelPortaliQ8BG16.
    SMRiQ8BG16. Positions 59-640.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000073829.

    PTM databases

    PhosphoSiteiQ8BG16.

    Proteomic databases

    MaxQBiQ8BG16.
    PaxDbiQ8BG16.
    PRIDEiQ8BG16.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
    ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
    GeneIDi103098.
    KEGGimmu:103098.
    UCSCiuc007gyj.2. mouse.

    Organism-specific databases

    CTDi55117.
    MGIiMGI:2143484. Slc6a15.

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00760000119044.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiQ8BG16.
    KOiK05048.
    OMAiAWVVVCL.
    OrthoDBiEOG793B71.
    PhylomeDBiQ8BG16.
    TreeFamiTF352709.

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

    Miscellaneous databases

    ChiTaRSiSlc6a15. mouse.
    NextBioi355783.
    PROiQ8BG16.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ8BG16.
    ExpressionAtlasiQ8BG16. baseline and differential.
    GenevisibleiQ8BG16. MM.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Mouse sodium-dependent orphan neurotransmitter transporter V73 cDNA."
      Liu Q.-R., Uhl G.R.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Characterization of orphan neurotransmitter transporter V73 genomic structure."
      Liu Q.-R., Uhl G.R.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ.
    3. "The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)."
      Broer A., Tietze N., Kowalczuk S., Chubb S., Munzinger M., Bak L.K., Broer S.
      Biochem. J. 393:421-430(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    7. "Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors."
      Drgonova J., Liu Q.R., Hall F.S., Krieger R.M., Uhl G.R.
      Brain Res. 1183:10-20(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiS6A15_MOUSE
    AccessioniPrimary (citable) accession number: Q8BG16
    Secondary accession number(s): Q333Y0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: March 1, 2003
    Last modified: June 24, 2015
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.