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Q8BG16

- S6A15_MOUSE

UniProt

Q8BG16 - S6A15_MOUSE

Protein

Sodium-dependent neutral amino acid transporter B(0)AT2

Gene

Slc6a15

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent By similarity.By similarity

    Kineticsi

    1. KM=195 µM for proline (in the presence of 100 mM NaCL)1 Publication
    2. KM=510 µM for proline (in the presence of 30 mM NaCL)1 Publication
    3. KM=790 µM for proline (in the presence of 3 mM NaCL)1 Publication
    4. KM=81 µM for leucine1 Publication
    5. KM=58 µM for isoleucine1 Publication
    6. KM=40 µM for methionine1 Publication
    7. KM=670 µM for alanine1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5.1 Publication

    GO - Molecular functioni

    1. neurotransmitter:sodium symporter activity Source: InterPro
    2. proline:sodium symporter activity Source: UniProtKB

    GO - Biological processi

    1. leucine transport Source: UniProtKB
    2. neutral amino acid transport Source: UniProtKB
    3. proline transmembrane transport Source: GOC
    4. proline transport Source: UniProtKB

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent neutral amino acid transporter B(0)AT2
    Alternative name(s):
    Sodium- and chloride-dependent neurotransmitter transporter NTT73
    Solute carrier family 6 member 15
    Transporter v7-3
    Gene namesi
    Name:Slc6a15
    Synonyms:B0at2, Ntt73
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:2143484. Slc6a15.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and fertile and fail to demonstrate any striking abnormality in motor and sensory functions. Other transporters could help to compensate for lost of SLC6A15.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Sodium-dependent neutral amino acid transporter B(0)AT2PRO_0000214799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551PhosphoserineBy similarity
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
    Modified residuei687 – 6871PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BG16.
    PRIDEiQ8BG16.

    PTM databases

    PhosphoSiteiQ8BG16.

    Expressioni

    Tissue specificityi

    Significant expressed in brain, lung and kidney. regions, the cortex, the cerebellum and the brain stem.1 Publication

    Developmental stagei

    Detected throughout development, starting with the pre-implantation embryo.1 Publication

    Gene expression databases

    ArrayExpressiQ8BG16.
    BgeeiQ8BG16.
    GenevestigatoriQ8BG16.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BG16.
    SMRiQ8BG16. Positions 59-640.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6969CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini163 – 22563ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini357 – 45296ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini643 – 72987CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei70 – 9021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei98 – 11720Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei226 – 24419Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei253 – 27018Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei306 – 32318Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35622Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei453 – 47220Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei496 – 51419Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei530 – 55021Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei571 – 59222Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei620 – 64223Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00750000117287.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiQ333Y0.
    KOiK05048.
    OMAiTPKLEMM.
    OrthoDBiEOG793B71.
    PhylomeDBiQ8BG16.
    TreeFamiTF352709.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BG16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EEVSKKSELI VDVQEEKDTD    50
    AEDGSEADDE RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL 100
    LPYLILLLVI GIPLFFLELS VGQRIRRGSI GVWNYISPKL GGIGFASCVV 150
    CYFVALYYNV IIGWTLFYFS QSFQQPLPWD QCPLVKNASH TYVEPECEQS 200
    SATTYYWYRE ALDITSSISD SGGLNWKMTV CLLVAWVMVC LAMIKGIQSS 250
    GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR 300
    EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL 350
    VVFAVLGFKA NIVNEKCISQ NSEMILKLLK MGNISWDVIP HHINLSAVTV 400
    EDYRLVYDII QKVKEEEFAV LHLNACQIED ELNKAVQGTG LAFIAFTEAM 450
    THFPASPFWS VMFFLMLINL GLGSMFGTIE GIITPIVDTF KVRKEILTVI 500
    CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE NIAVSFVYGI 550
    DKFIEDLTDM LGFAPSKYYY YMWKYISPLM LLTLLIASIV NMGLSPPGYN 600
    AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFIIR RCNLIDDSSG 650
    NLASVTYKRG RVLKEPVNLE GDDASLIHGK IPSEMSSPNF GKNIYRKQSG 700
    SPTLDTAPNG RYGIGYLMAD MPDMPESDL 729
    Length:729
    Mass (Da):81,792
    Last modified:March 1, 2003 - v1
    Checksum:iD336735E9D1E9B97
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY149280 mRNA. Translation: AAN75437.1.
    AY149281 mRNA. Translation: AAN75438.1.
    AY149282 Genomic DNA. Translation: AAN75439.1.
    AM085111 mRNA. Translation: CAJ29896.1.
    AK036136 mRNA. Translation: BAC29315.1.
    CH466539 Genomic DNA. Translation: EDL21676.1.
    CH466539 Genomic DNA. Translation: EDL21677.1.
    BC076593 mRNA. Translation: AAH76593.1.
    CCDSiCCDS24156.1.
    RefSeqiNP_001239259.1. NM_001252330.1.
    NP_780537.1. NM_175328.3.
    XP_006513045.1. XM_006512982.1.
    XP_006513046.1. XM_006512983.1.
    XP_006513047.1. XM_006512984.1.
    UniGeneiMm.458408.

    Genome annotation databases

    EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
    ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
    GeneIDi103098.
    KEGGimmu:103098.
    UCSCiuc007gyj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY149280 mRNA. Translation: AAN75437.1 .
    AY149281 mRNA. Translation: AAN75438.1 .
    AY149282 Genomic DNA. Translation: AAN75439.1 .
    AM085111 mRNA. Translation: CAJ29896.1 .
    AK036136 mRNA. Translation: BAC29315.1 .
    CH466539 Genomic DNA. Translation: EDL21676.1 .
    CH466539 Genomic DNA. Translation: EDL21677.1 .
    BC076593 mRNA. Translation: AAH76593.1 .
    CCDSi CCDS24156.1.
    RefSeqi NP_001239259.1. NM_001252330.1.
    NP_780537.1. NM_175328.3.
    XP_006513045.1. XM_006512982.1.
    XP_006513046.1. XM_006512983.1.
    XP_006513047.1. XM_006512984.1.
    UniGenei Mm.458408.

    3D structure databases

    ProteinModelPortali Q8BG16.
    SMRi Q8BG16. Positions 59-640.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BG16.

    Proteomic databases

    PaxDbi Q8BG16.
    PRIDEi Q8BG16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000074204 ; ENSMUSP00000073829 ; ENSMUSG00000019894 .
    ENSMUST00000179636 ; ENSMUSP00000136676 ; ENSMUSG00000019894 .
    GeneIDi 103098.
    KEGGi mmu:103098.
    UCSCi uc007gyj.2. mouse.

    Organism-specific databases

    CTDi 55117.
    MGIi MGI:2143484. Slc6a15.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00750000117287.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    InParanoidi Q333Y0.
    KOi K05048.
    OMAi TPKLEMM.
    OrthoDBi EOG793B71.
    PhylomeDBi Q8BG16.
    TreeFami TF352709.

    Miscellaneous databases

    NextBioi 355783.
    PROi Q8BG16.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BG16.
    Bgeei Q8BG16.
    Genevestigatori Q8BG16.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse sodium-dependent orphan neurotransmitter transporter V73 cDNA."
      Liu Q.-R., Uhl G.R.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Characterization of orphan neurotransmitter transporter V73 genomic structure."
      Liu Q.-R., Uhl G.R.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ.
    3. "The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)."
      Broer A., Tietze N., Kowalczuk S., Chubb S., Munzinger M., Bak L.K., Broer S.
      Biochem. J. 393:421-430(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    7. "Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors."
      Drgonova J., Liu Q.R., Hall F.S., Krieger R.M., Uhl G.R.
      Brain Res. 1183:10-20(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiS6A15_MOUSE
    AccessioniPrimary (citable) accession number: Q8BG16
    Secondary accession number(s): Q333Y0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3