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Protein

Sodium-dependent neutral amino acid transporter B(0)AT2

Gene

Slc6a15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent (By similarity).By similarity

Kineticsi

  1. KM=195 µM for proline (in the presence of 100 mM NaCL)1 Publication
  2. KM=510 µM for proline (in the presence of 30 mM NaCL)1 Publication
  3. KM=790 µM for proline (in the presence of 3 mM NaCL)1 Publication
  4. KM=81 µM for leucine1 Publication
  5. KM=58 µM for isoleucine1 Publication
  6. KM=40 µM for methionine1 Publication
  7. KM=670 µM for alanine1 Publication

pH dependencei

Optimum pH is 7.5-8.5.1 Publication

GO - Molecular functioni

  1. neurotransmitter:sodium symporter activity Source: InterPro
  2. proline:sodium symporter activity Source: UniProtKB

GO - Biological processi

  1. leucine transport Source: UniProtKB
  2. neutral amino acid transport Source: UniProtKB
  3. proline transmembrane transport Source: GOC
  4. proline transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent neutral amino acid transporter B(0)AT2
Alternative name(s):
Sodium- and chloride-dependent neurotransmitter transporter NTT73
Solute carrier family 6 member 15
Transporter v7-3
Gene namesi
Name:Slc6a15
Synonyms:B0at2, Ntt73
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2143484. Slc6a15.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei70 – 9021Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei98 – 11720Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei142 – 16221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini163 – 22563ExtracellularSequence AnalysisAdd
BLAST
Transmembranei226 – 24419Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei253 – 27018Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei306 – 32318Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei335 – 35622Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini357 – 45296ExtracellularSequence AnalysisAdd
BLAST
Transmembranei453 – 47220Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei496 – 51419Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei530 – 55021Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei571 – 59222Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei620 – 64223Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini643 – 72987CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile and fail to demonstrate any striking abnormality in motor and sensory functions. Other transporters could help to compensate for lost of SLC6A15.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Sodium-dependent neutral amino acid transporter B(0)AT2PRO_0000214799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphoserineBy similarity
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
Modified residuei687 – 6871PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BG16.
PaxDbiQ8BG16.
PRIDEiQ8BG16.

PTM databases

PhosphoSiteiQ8BG16.

Expressioni

Tissue specificityi

Significant expressed in brain, lung and kidney. regions, the cortex, the cerebellum and the brain stem.1 Publication

Developmental stagei

Detected throughout development, starting with the pre-implantation embryo.1 Publication

Gene expression databases

BgeeiQ8BG16.
ExpressionAtlasiQ8BG16. baseline and differential.
GenevestigatoriQ8BG16.

Structurei

3D structure databases

ProteinModelPortaliQ8BG16.
SMRiQ8BG16. Positions 59-640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000119044.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ8BG16.
KOiK05048.
OMAiAWVVVCL.
OrthoDBiEOG793B71.
PhylomeDBiQ8BG16.
TreeFamiTF352709.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BG16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EEVSKKSELI VDVQEEKDTD
60 70 80 90 100
AEDGSEADDE RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL
110 120 130 140 150
LPYLILLLVI GIPLFFLELS VGQRIRRGSI GVWNYISPKL GGIGFASCVV
160 170 180 190 200
CYFVALYYNV IIGWTLFYFS QSFQQPLPWD QCPLVKNASH TYVEPECEQS
210 220 230 240 250
SATTYYWYRE ALDITSSISD SGGLNWKMTV CLLVAWVMVC LAMIKGIQSS
260 270 280 290 300
GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR
310 320 330 340 350
EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL
360 370 380 390 400
VVFAVLGFKA NIVNEKCISQ NSEMILKLLK MGNISWDVIP HHINLSAVTV
410 420 430 440 450
EDYRLVYDII QKVKEEEFAV LHLNACQIED ELNKAVQGTG LAFIAFTEAM
460 470 480 490 500
THFPASPFWS VMFFLMLINL GLGSMFGTIE GIITPIVDTF KVRKEILTVI
510 520 530 540 550
CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE NIAVSFVYGI
560 570 580 590 600
DKFIEDLTDM LGFAPSKYYY YMWKYISPLM LLTLLIASIV NMGLSPPGYN
610 620 630 640 650
AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFIIR RCNLIDDSSG
660 670 680 690 700
NLASVTYKRG RVLKEPVNLE GDDASLIHGK IPSEMSSPNF GKNIYRKQSG
710 720
SPTLDTAPNG RYGIGYLMAD MPDMPESDL
Length:729
Mass (Da):81,792
Last modified:March 1, 2003 - v1
Checksum:iD336735E9D1E9B97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY149280 mRNA. Translation: AAN75437.1.
AY149281 mRNA. Translation: AAN75438.1.
AY149282 Genomic DNA. Translation: AAN75439.1.
AM085111 mRNA. Translation: CAJ29896.1.
AK036136 mRNA. Translation: BAC29315.1.
CH466539 Genomic DNA. Translation: EDL21676.1.
CH466539 Genomic DNA. Translation: EDL21677.1.
BC076593 mRNA. Translation: AAH76593.1.
CCDSiCCDS24156.1.
RefSeqiNP_001239259.1. NM_001252330.1.
NP_780537.1. NM_175328.3.
XP_006513045.1. XM_006512982.2.
XP_006513046.1. XM_006512983.2.
XP_006513047.1. XM_006512984.2.
UniGeneiMm.458408.

Genome annotation databases

EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
GeneIDi103098.
KEGGimmu:103098.
UCSCiuc007gyj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY149280 mRNA. Translation: AAN75437.1.
AY149281 mRNA. Translation: AAN75438.1.
AY149282 Genomic DNA. Translation: AAN75439.1.
AM085111 mRNA. Translation: CAJ29896.1.
AK036136 mRNA. Translation: BAC29315.1.
CH466539 Genomic DNA. Translation: EDL21676.1.
CH466539 Genomic DNA. Translation: EDL21677.1.
BC076593 mRNA. Translation: AAH76593.1.
CCDSiCCDS24156.1.
RefSeqiNP_001239259.1. NM_001252330.1.
NP_780537.1. NM_175328.3.
XP_006513045.1. XM_006512982.2.
XP_006513046.1. XM_006512983.2.
XP_006513047.1. XM_006512984.2.
UniGeneiMm.458408.

3D structure databases

ProteinModelPortaliQ8BG16.
SMRiQ8BG16. Positions 59-640.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8BG16.

Proteomic databases

MaxQBiQ8BG16.
PaxDbiQ8BG16.
PRIDEiQ8BG16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
GeneIDi103098.
KEGGimmu:103098.
UCSCiuc007gyj.2. mouse.

Organism-specific databases

CTDi55117.
MGIiMGI:2143484. Slc6a15.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000119044.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ8BG16.
KOiK05048.
OMAiAWVVVCL.
OrthoDBiEOG793B71.
PhylomeDBiQ8BG16.
TreeFamiTF352709.

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

ChiTaRSiSlc6a15. mouse.
NextBioi355783.
PROiQ8BG16.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BG16.
ExpressionAtlasiQ8BG16. baseline and differential.
GenevestigatoriQ8BG16.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse sodium-dependent orphan neurotransmitter transporter V73 cDNA."
    Liu Q.-R., Uhl G.R.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Characterization of orphan neurotransmitter transporter V73 genomic structure."
    Liu Q.-R., Uhl G.R.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
  3. "The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)."
    Broer A., Tietze N., Kowalczuk S., Chubb S., Munzinger M., Bak L.K., Broer S.
    Biochem. J. 393:421-430(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  7. "Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors."
    Drgonova J., Liu Q.R., Hall F.S., Krieger R.M., Uhl G.R.
    Brain Res. 1183:10-20(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiS6A15_MOUSE
AccessioniPrimary (citable) accession number: Q8BG16
Secondary accession number(s): Q333Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.