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Q8BG16 (S6A15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent neutral amino acid transporter B(0)AT2
Alternative name(s):
Sodium- and chloride-dependent neurotransmitter transporter NTT73
Solute carrier family 6 member 15
Transporter v7-3
Gene names
Name:Slc6a15
Synonyms:B0at2, Ntt73
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent By similarity. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Significant expressed in brain, lung and kidney. regions, the cortex, the cerebellum and the brain stem. Ref.3

Developmental stage

Detected throughout development, starting with the pre-implantation embryo. Ref.3

Disruption phenotype

Mice are viable and fertile and fail to demonstrate any striking abnormality in motor and sensory functions. Other transporters could help to compensate for lost of SLC6A15. Ref.7

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=195 µM for proline (in the presence of 100 mM NaCL) Ref.3

KM=510 µM for proline (in the presence of 30 mM NaCL)

KM=790 µM for proline (in the presence of 3 mM NaCL)

KM=81 µM for leucine

KM=58 µM for isoleucine

KM=40 µM for methionine

KM=670 µM for alanine

pH dependence:

Optimum pH is 7.5-8.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Sodium-dependent neutral amino acid transporter B(0)AT2
PRO_0000214799

Regions

Topological domain1 – 6969Cytoplasmic Potential
Transmembrane70 – 9021Helical; Name=1; Potential
Transmembrane98 – 11720Helical; Name=2; Potential
Transmembrane142 – 16221Helical; Name=3; Potential
Topological domain163 – 22563Extracellular Potential
Transmembrane226 – 24419Helical; Name=4; Potential
Transmembrane253 – 27018Helical; Name=5; Potential
Transmembrane306 – 32318Helical; Name=6; Potential
Transmembrane335 – 35622Helical; Name=7; Potential
Topological domain357 – 45296Extracellular Potential
Transmembrane453 – 47220Helical; Name=8; Potential
Transmembrane496 – 51419Helical; Name=9; Potential
Transmembrane530 – 55021Helical; Name=10; Potential
Transmembrane571 – 59222Helical; Name=11; Potential
Transmembrane620 – 64223Helical; Name=12; Potential
Topological domain643 – 72987Cytoplasmic Potential

Amino acid modifications

Modified residue551Phosphoserine By similarity
Modified residue6871Phosphoserine By similarity
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2761N-linked (GlcNAc...) Potential
Glycosylation3831N-linked (GlcNAc...) Potential
Glycosylation3941N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8BG16 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D336735E9D1E9B97

FASTA72981,792
        10         20         30         40         50         60 
MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EEVSKKSELI VDVQEEKDTD AEDGSEADDE 

        70         80         90        100        110        120 
RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL LPYLILLLVI GIPLFFLELS 

       130        140        150        160        170        180 
VGQRIRRGSI GVWNYISPKL GGIGFASCVV CYFVALYYNV IIGWTLFYFS QSFQQPLPWD 

       190        200        210        220        230        240 
QCPLVKNASH TYVEPECEQS SATTYYWYRE ALDITSSISD SGGLNWKMTV CLLVAWVMVC 

       250        260        270        280        290        300 
LAMIKGIQSS GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR 

       310        320        330        340        350        360 
EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL VVFAVLGFKA 

       370        380        390        400        410        420 
NIVNEKCISQ NSEMILKLLK MGNISWDVIP HHINLSAVTV EDYRLVYDII QKVKEEEFAV 

       430        440        450        460        470        480 
LHLNACQIED ELNKAVQGTG LAFIAFTEAM THFPASPFWS VMFFLMLINL GLGSMFGTIE 

       490        500        510        520        530        540 
GIITPIVDTF KVRKEILTVI CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE 

       550        560        570        580        590        600 
NIAVSFVYGI DKFIEDLTDM LGFAPSKYYY YMWKYISPLM LLTLLIASIV NMGLSPPGYN 

       610        620        630        640        650        660 
AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFIIR RCNLIDDSSG NLASVTYKRG 

       670        680        690        700        710        720 
RVLKEPVNLE GDDASLIHGK IPSEMSSPNF GKNIYRKQSG SPTLDTAPNG RYGIGYLMAD 


MPDMPESDL 

« Hide

References

« Hide 'large scale' references
[1]"Mouse sodium-dependent orphan neurotransmitter transporter V73 cDNA."
Liu Q.-R., Uhl G.R.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"Characterization of orphan neurotransmitter transporter V73 genomic structure."
Liu Q.-R., Uhl G.R.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: 129/SvJ.
[3]"The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)."
Broer A., Tietze N., Kowalczuk S., Chubb S., Munzinger M., Bak L.K., Broer S.
Biochem. J. 393:421-430(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Brain.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[7]"Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors."
Drgonova J., Liu Q.R., Hall F.S., Krieger R.M., Uhl G.R.
Brain Res. 1183:10-20(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY149280 mRNA. Translation: AAN75437.1.
AY149281 mRNA. Translation: AAN75438.1.
AY149282 Genomic DNA. Translation: AAN75439.1.
AM085111 mRNA. Translation: CAJ29896.1.
AK036136 mRNA. Translation: BAC29315.1.
CH466539 Genomic DNA. Translation: EDL21676.1.
CH466539 Genomic DNA. Translation: EDL21677.1.
BC076593 mRNA. Translation: AAH76593.1.
RefSeqNP_001239259.1. NM_001252330.1.
NP_780537.1. NM_175328.3.
XP_006513045.1. XM_006512982.1.
XP_006513046.1. XM_006512983.1.
XP_006513047.1. XM_006512984.1.
UniGeneMm.458408.

3D structure databases

ProteinModelPortalQ8BG16.
SMRQ8BG16. Positions 59-640.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BG16.

Proteomic databases

PaxDbQ8BG16.
PRIDEQ8BG16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074204; ENSMUSP00000073829; ENSMUSG00000019894.
ENSMUST00000179636; ENSMUSP00000136676; ENSMUSG00000019894.
GeneID103098.
KEGGmmu:103098.
UCSCuc007gyj.2. mouse.

Organism-specific databases

CTD55117.
MGIMGI:2143484. Slc6a15.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00750000117287.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidQ333Y0.
KOK05048.
OMANSCQIED.
OrthoDBEOG793B71.
PhylomeDBQ8BG16.
TreeFamTF352709.

Gene expression databases

ArrayExpressQ8BG16.
BgeeQ8BG16.
GenevestigatorQ8BG16.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio355783.
PROQ8BG16.
SOURCESearch...

Entry information

Entry nameS6A15_MOUSE
AccessionPrimary (citable) accession number: Q8BG16
Secondary accession number(s): Q333Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot