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Protein

Phospholipase D4

Gene

Pld4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei212 – 2121PROSITE-ProRule annotation
Active sitei214 – 2141PROSITE-ProRule annotation
Active sitei219 – 2191PROSITE-ProRule annotation
Active sitei426 – 4261PROSITE-ProRule annotation
Active sitei428 – 4281PROSITE-ProRule annotation
Active sitei433 – 4331PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • hematopoietic progenitor cell differentiation Source: MGI
  • lipid catabolic process Source: UniProtKB-KW
  • phagocytosis Source: MGI
  • phosphatidylglycerol biosynthetic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1483148. Synthesis of PG.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D4 (EC:3.1.4.4)
Short name:
PLD 4
Alternative name(s):
Choline phosphatase 4
Phosphatidylcholine-hydrolyzing phospholipase D4
Gene namesi
Name:Pld4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2144765. Pld4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei37 – 5721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: MGI
  • phagocytic vesicle Source: MGI
  • trans-Golgi network membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Phospholipase D4PRO_0000280334Add
BLAST

Proteomic databases

MaxQBiQ8BG07.
PaxDbiQ8BG07.
PeptideAtlasiQ8BG07.
PRIDEiQ8BG07.

PTM databases

PhosphoSiteiQ8BG07.

Expressioni

Gene expression databases

BgeeiQ8BG07.
CleanExiMM_PLD4.
GenevisibleiQ8BG07. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067002.

Structurei

3D structure databases

ProteinModelPortaliQ8BG07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 23428PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini421 – 44727PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phospholipase D family.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3603. Eukaryota.
ENOG410XQZ4. LUCA.
GeneTreeiENSGT00390000009798.
HOGENOMiHOG000293407.
HOVERGENiHBG052880.
InParanoidiQ8BG07.
KOiK16860.
OMAiLCPHGRT.
OrthoDBiEOG70KGP9.
PhylomeDBiQ8BG07.
TreeFamiTF313378.

Family and domain databases

InterProiIPR032803. PLDc_3.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PfamiPF00614. PLDc. 2 hits.
PF13918. PLDc_3. 1 hit.
[Graphical view]
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BG07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKKKEHPEM RIPLQTAVEV SDWPCSTSHD PHSGLGMVLG MLAVLGLSSV
60 70 80 90 100
TLILFLWQGA TSFTSHRMFP EEVPSWSWET LKGDAEQQNN SCQLILVESI
110 120 130 140 150
PEDLPFAAGS PTAQPLAQAW LQLLDTARES VHIASYYWSL TGLDIGVNDS
160 170 180 190 200
SSRQGEALLQ KFQQLLLRNI SVVVATHSPT LAKTSTDLQV LAAHGAQIRQ
210 220 230 240 250
VPMKQLTGGV LHSKFWVVDG RHIYVGSANM DWRSLTQVKE LGAIIYNCSN
260 270 280 290 300
LAQDLEKTFQ TYWVLGTPQA VLPKTWPRNF SSHINRFHPL RGPFDGVPTT
310 320 330 340 350
AYFSASPPSL CPHGRTRDLD AVLGVMEGAR QFIYVSVMEY FPTTRFTHHA
360 370 380 390 400
RYWPVLDNAL RAAALNKGVH VRLLVSCWFN TDPTMFAYLR SLQAFSNPSA
410 420 430 440 450
GISVDVKVFI VPVGNHSNIP FSRVNHSKFM VTDKTAYVGT SNWSEDYFSH
460 470 480 490 500
TAGVGLIVSQ KTPRAQPGAT TVQEQLRQLF ERDWSSHYAM DLDRQVPSQD

CVW
Length:503
Mass (Da):56,154
Last modified:March 1, 2003 - v1
Checksum:iD72EB7DEBAC9598F
GO
Isoform 2 (identifier: Q8BG07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MDKKKEHPE → M

Note: No experimental confirmation available.
Show »
Length:495
Mass (Da):55,162
Checksum:iA0FCEEDA73B5BAED
GO

Sequence cautioni

The sequence BAC32379.1 differs from that shown. Reason: Frameshift at position 443. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231I → V in AAH58565 (PubMed:15489334).Curated
Sequence conflicti294 – 2941F → L in BAE41745 (PubMed:16141072).Curated
Sequence conflicti450 – 4501H → Y in BAE41745 (PubMed:16141072).Curated
Sequence conflicti452 – 4521A → G in BAC32379 (PubMed:16141072).Curated
Sequence conflicti455 – 4551G → V in BAC32379 (PubMed:16141072).Curated
Sequence conflicti483 – 4831D → G in BAC32379 (PubMed:16141072).Curated
Sequence conflicti489 – 4891A → G in BAC32379 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99MDKKKEHPE → M in isoform 2. 1 PublicationVSP_023630

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045459 mRNA. Translation: BAC32379.1. Frameshift.
AK079549 mRNA. Translation: BAC37681.1.
AK079878 mRNA. Translation: BAC37771.1.
AK151551 mRNA. Translation: BAE30496.1.
AK170362 mRNA. Translation: BAE41745.1.
BC058565 mRNA. Translation: AAH58565.1.
CCDSiCCDS26195.1. [Q8BG07-1]
RefSeqiNP_849242.1. NM_178911.4. [Q8BG07-1]
XP_011242277.1. XM_011243975.1. [Q8BG07-2]
UniGeneiMm.203915.

Genome annotation databases

EnsembliENSMUST00000063888; ENSMUSP00000067002; ENSMUSG00000052160. [Q8BG07-1]
GeneIDi104759.
KEGGimmu:104759.
UCSCiuc007pfc.1. mouse. [Q8BG07-1]
uc007pfd.1. mouse. [Q8BG07-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045459 mRNA. Translation: BAC32379.1. Frameshift.
AK079549 mRNA. Translation: BAC37681.1.
AK079878 mRNA. Translation: BAC37771.1.
AK151551 mRNA. Translation: BAE30496.1.
AK170362 mRNA. Translation: BAE41745.1.
BC058565 mRNA. Translation: AAH58565.1.
CCDSiCCDS26195.1. [Q8BG07-1]
RefSeqiNP_849242.1. NM_178911.4. [Q8BG07-1]
XP_011242277.1. XM_011243975.1. [Q8BG07-2]
UniGeneiMm.203915.

3D structure databases

ProteinModelPortaliQ8BG07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067002.

PTM databases

PhosphoSiteiQ8BG07.

Proteomic databases

MaxQBiQ8BG07.
PaxDbiQ8BG07.
PeptideAtlasiQ8BG07.
PRIDEiQ8BG07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063888; ENSMUSP00000067002; ENSMUSG00000052160. [Q8BG07-1]
GeneIDi104759.
KEGGimmu:104759.
UCSCiuc007pfc.1. mouse. [Q8BG07-1]
uc007pfd.1. mouse. [Q8BG07-2]

Organism-specific databases

CTDi122618.
MGIiMGI:2144765. Pld4.

Phylogenomic databases

eggNOGiKOG3603. Eukaryota.
ENOG410XQZ4. LUCA.
GeneTreeiENSGT00390000009798.
HOGENOMiHOG000293407.
HOVERGENiHBG052880.
InParanoidiQ8BG07.
KOiK16860.
OMAiLCPHGRT.
OrthoDBiEOG70KGP9.
PhylomeDBiQ8BG07.
TreeFamiTF313378.

Enzyme and pathway databases

ReactomeiR-MMU-1483148. Synthesis of PG.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

ChiTaRSiPld4. mouse.
PROiQ8BG07.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BG07.
CleanExiMM_PLD4.
GenevisibleiQ8BG07. MM.

Family and domain databases

InterProiIPR032803. PLDc_3.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PfamiPF00614. PLDc. 2 hits.
PF13918. PLDc_3. 1 hit.
[Graphical view]
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Corpora quadrigemina, Hypothalamus and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiPLD4_MOUSE
AccessioniPrimary (citable) accession number: Q8BG07
Secondary accession number(s): Q3TD59
, Q3UA19, Q6PDR0, Q8BR69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.