Reviewed, UniProtKB/Swiss-Prot Q8BG07 (PLD4_MOUSE)

Names and origin

Protein names

Recommended name:
    Phospholipase D4
      Short name=PLD 4
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 4
    Phosphatidylcholine-hydrolyzing phospholipase D4

Gene names

Name:

Pld4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.
Sequence caution	The sequence BAC32379.1 differs from that shown. Reason: Frameshift at position 443.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BG07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BG07-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MDKKKEHPE → M

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Phospholipase D4		PRO_0000280334
Regions
	Transmembrane	37 – 57	21	Potential
	Domain	207 – 234	28	PLD phosphodiesterase 1
	Domain	421 – 447	27	PLD phosphodiesterase 2
Sites
	Active site	212	1	Potential
	Active site	214	1	Potential
	Active site	219	1	Potential
	Active site	426	1	Potential
	Active site	428	1	Potential
	Active site	433	1	Potential
Amino acid modifications
	Modified residue	471	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 9	9	MDKKKEHPE → M in isoform 2.		VSP_023630
Experimental info
	Sequence conflict	223	1	I → V in AAH58565. Ref.2
	Sequence conflict	294	1	F → L in BAE41745. Ref.1
	Sequence conflict	450	1	H → Y in BAE41745. Ref.1
	Sequence conflict	452	1	A → G in BAC32379. Ref.1
	Sequence conflict	455	1	G → V in BAC32379. Ref.1
	Sequence conflict	483	1	D → G in BAC32379. Ref.1
	Sequence conflict	489	1	A → G in BAC32379. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D72EB7DEBAC9598F	FASTA	503	56,154
	10 20 30 40 50 60 MDKKKEHPEM RIPLQTAVEV SDWPCSTSHD PHSGLGMVLG MLAVLGLSSV TLILFLWQGA 70 80 90 100 110 120 TSFTSHRMFP EEVPSWSWET LKGDAEQQNN SCQLILVESI PEDLPFAAGS PTAQPLAQAW 130 140 150 160 170 180 LQLLDTARES VHIASYYWSL TGLDIGVNDS SSRQGEALLQ KFQQLLLRNI SVVVATHSPT 190 200 210 220 230 240 LAKTSTDLQV LAAHGAQIRQ VPMKQLTGGV LHSKFWVVDG RHIYVGSANM DWRSLTQVKE 250 260 270 280 290 300 LGAIIYNCSN LAQDLEKTFQ TYWVLGTPQA VLPKTWPRNF SSHINRFHPL RGPFDGVPTT 310 320 330 340 350 360 AYFSASPPSL CPHGRTRDLD AVLGVMEGAR QFIYVSVMEY FPTTRFTHHA RYWPVLDNAL 370 380 390 400 410 420 RAAALNKGVH VRLLVSCWFN TDPTMFAYLR SLQAFSNPSA GISVDVKVFI VPVGNHSNIP 430 440 450 460 470 480 FSRVNHSKFM VTDKTAYVGT SNWSEDYFSH TAGVGLIVSQ KTPRAQPGAT TVQEQLRQLF 490 500 ERDWSSHYAM DLDRQVPSQD CVW
	Isoform 2. Checksum: A0FCEEDA73B5BAED	FASTA	495	55,162
	10 20 30 40 50 60 MMRIPLQTAV EVSDWPCSTS HDPHSGLGMV LGMLAVLGLS SVTLILFLWQ GATSFTSHRM 70 80 90 100 110 120 FPEEVPSWSW ETLKGDAEQQ NNSCQLILVE SIPEDLPFAA GSPTAQPLAQ AWLQLLDTAR 130 140 150 160 170 180 ESVHIASYYW SLTGLDIGVN DSSSRQGEAL LQKFQQLLLR NISVVVATHS PTLAKTSTDL 190 200 210 220 230 240 QVLAAHGAQI RQVPMKQLTG GVLHSKFWVV DGRHIYVGSA NMDWRSLTQV KELGAIIYNC 250 260 270 280 290 300 SNLAQDLEKT FQTYWVLGTP QAVLPKTWPR NFSSHINRFH PLRGPFDGVP TTAYFSASPP 310 320 330 340 350 360 SLCPHGRTRD LDAVLGVMEG ARQFIYVSVM EYFPTTRFTH HARYWPVLDN ALRAAALNKG 370 380 390 400 410 420 VHVRLLVSCW FNTDPTMFAY LRSLQAFSNP SAGISVDVKV FIVPVGNHSN IPFSRVNHSK 430 440 450 460 470 480 FMVTDKTAYV GTSNWSEDYF SHTAGVGLIV SQKTPRAQPG ATTVQEQLRQ LFERDWSSHY 490 AMDLDRQVPS QDCVW

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Bone marrow, Corpora quadrigemina, Hypothalamus and Thymus.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N-3. Tissue: Mammary tumor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK045459 mRNA. Translation: BAC32379.1. Frameshift. AK079549 mRNA. Translation: BAC37681.1. AK079878 mRNA. Translation: BAC37771.1. AK151551 mRNA. Translation: BAE30496.1. AK170362 mRNA. Translation: BAE41745.1. BC058565 mRNA. Translation: AAH58565.1.
IPI	IPI00221418. IPI00831033.
RefSeq	NP_849242.1.
UniGene	Mm.203915
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8BG07.
Genome annotation databases
Ensembl	ENSMUSG00000052160. Mus musculus. [Contig view]
GeneID	104759.
KEGG	mmu:104759.
Organism-specific databases
MGI	MGI:2144765. Pld4.
Phylogenomic databases
HOVERGEN	Q8BG07.
OMA	Q8BG07. VNDSSSQ.
Enzyme and pathway databases
BRENDA	3.1.4.4. 244.
Gene expression databases
ArrayExpress	Q8BG07.
Bgee	Q8BG07.
CleanEx	MM_PLD4.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357270.
SOURCE	Search...

Entry information

Entry name

PLD4_MOUSE

Accession

Primary (citable) accession number: Q8BG07
Secondary accession number(s): Q3TD59 , Q3UA19, Q6PDR0, Q8BR69

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)