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Protein

Isopentenyl-diphosphate delta-isomerase 2

Gene

Idi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).By similarity

Catalytic activityi

Isopentenyl diphosphate = dimethylallyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Isopentenyl-diphosphate Delta-isomerase 1 (Idi1), Isopentenyl-diphosphate delta-isomerase 2 (Idi2)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi40MagnesiumBy similarity1
Metal bindingi51MagnesiumBy similarity1
Binding sitei70SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei86By similarity1
Binding sitei87SubstrateBy similarity1
Metal bindingi146MagnesiumBy similarity1
Active sitei148By similarity1
Metal bindingi148MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
UniPathwayiUPA00059; UER00104.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopentenyl-diphosphate delta-isomerase 2 (EC:5.3.3.2)
Alternative name(s):
Isopentenyl pyrophosphate isomerase 2
Short name:
IPP isomerase 2
Short name:
IPPI2
Gene namesi
Name:Idi2
Synonyms:Gm9745
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:3704398. Gm9745.
MGI:2444315. Idi2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004208671 – 227Isopentenyl-diphosphate delta-isomerase 2Add BLAST227

Proteomic databases

PaxDbiQ8BFZ6.
PRIDEiQ8BFZ6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021148.
GenevisibleiQ8BFZ6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036621.

Structurei

3D structure databases

ProteinModelPortaliQ8BFZ6.
SMRiQ8BFZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 199Nudix hydrolasePROSITE-ProRule annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 227Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the IPP isomerase type 1 family.Curated

Phylogenomic databases

eggNOGiKOG0142. Eukaryota.
COG1443. LUCA.
GeneTreeiENSGT00390000008527.
HOGENOMiHOG000274106.
HOVERGENiHBG002995.
InParanoidiQ8BFZ6.
KOiK01823.
OMAiFRAFSAM.
OrthoDBiEOG091G0LWP.
PhylomeDBiQ8BFZ6.
TreeFamiTF300129.

Family and domain databases

CDDicd02885. IPP_Isomerase. 1 hit.
InterProiView protein in InterPro
IPR011876. IsopentenylPP_isomerase_typ1.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
PANTHERiPTHR10885. PTHR10885. 1 hit.
PfamiView protein in Pfam
PF00293. NUDIX. 1 hit.
PIRSFiPIRSF018427. Isopntndiph_ism. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR02150. IPP_isom_1. 1 hit.
PROSITEiView protein in PROSITE
PS51462. NUDIX. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BFZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQASKTHLD ELQLKRLEEM CIVIDKQDQI IGADTKKNCH LMENINKGLL
60 70 80 90 100
HRAFSVVLFN MKNELLVQQR ADAKYTFPGH FTDSCSSHPL YVPEELEEKD
110 120 130 140 150
ALGVRRAALR RLQAELGISQ DQISIKDIIF MTRKYHKCQS DAIWGEHEIG
160 170 180 190 200
YLLLVRKDLM LNPDTREVRR CCYMSQKDVQ ELLDREARGE EKITPWFRSM
210 220
VEDFLFSWWP HLEDVSSFVE PDKIYGL
Length:227
Mass (Da):26,665
Last modified:March 1, 2003 - v1
Checksum:iDACEA662BA4209A0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20M → I in AAH99586 (PubMed:15489334).Curated1
Sequence conflicti186E → G in AAH99586 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263178 mRNA. Translation: AAP21674.1.
AK029364 mRNA. Translation: BAC26418.1.
AK081319 mRNA. Translation: BAC38194.1.
AC124732 Genomic DNA. No translation available.
BC099586 mRNA. Translation: AAH99586.1.
BC125310 mRNA. Translation: AAI25311.1.
BC138100 mRNA. Translation: AAI38101.1.
CCDSiCCDS26235.1.
RefSeqiNP_796171.1. NM_177197.4.
UniGeneiMm.313743.

Genome annotation databases

EnsembliENSMUST00000021573; ENSMUSP00000021573; ENSMUSG00000021148.
ENSMUST00000038598; ENSMUSP00000036621; ENSMUSG00000033520.
ENSMUST00000187196; ENSMUSP00000139705; ENSMUSG00000021148.
GeneIDi320581.
KEGGimmu:320581.
UCSCiuc007pko.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiIDI2_MOUSE
AccessioniPrimary (citable) accession number: Q8BFZ6
Secondary accession number(s): Q4FZF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: March 1, 2003
Last modified: August 30, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families