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Protein

Isopentenyl-diphosphate delta-isomerase 2

Gene

Idi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).By similarity

Catalytic activityi

Isopentenyl diphosphate = dimethylallyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Isopentenyl-diphosphate Delta-isomerase 1 (Idi1), Isopentenyl-diphosphate delta-isomerase 2 (Idi2)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Metal bindingi40 – 401MagnesiumBy similarity
Metal bindingi51 – 511MagnesiumBy similarity
Binding sitei70 – 701SubstrateBy similarity
Binding sitei74 – 741SubstrateBy similarity
Active sitei86 – 861By similarity
Binding sitei87 – 871SubstrateBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Active sitei148 – 1481By similarity
Metal bindingi148 – 1481MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
UniPathwayiUPA00059; UER00104.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopentenyl-diphosphate delta-isomerase 2 (EC:5.3.3.2)
Alternative name(s):
Isopentenyl pyrophosphate isomerase 2
Short name:
IPP isomerase 2
Short name:
IPPI2
Gene namesi
Name:Idi2
Synonyms:Gm9745
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:3704398. Gm9745.
MGI:2444315. Idi2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Isopentenyl-diphosphate delta-isomerase 2PRO_0000420867Add
BLAST

Proteomic databases

PaxDbiQ8BFZ6.

Expressioni

Gene expression databases

BgeeiQ8BFZ6.
GenevisibleiQ8BFZ6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036621.

Structurei

3D structure databases

ProteinModelPortaliQ8BFZ6.
SMRiQ8BFZ6. Positions 9-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 199151Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi225 – 2273Microbody targeting signalSequence analysis

Sequence similaritiesi

Belongs to the IPP isomerase type 1 family.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0142. Eukaryota.
COG1443. LUCA.
GeneTreeiENSGT00390000008527.
HOGENOMiHOG000274106.
HOVERGENiHBG002995.
InParanoidiQ8BFZ6.
KOiK01823.
OMAiPREVKSY.
OrthoDBiEOG7H4DVK.
PhylomeDBiQ8BFZ6.
TreeFamiTF300129.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR011876. IsopentenylPP_isomerase_typ1.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PANTHERiPTHR10885. PTHR10885. 1 hit.
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PIRSFiPIRSF018427. Isopntndiph_ism. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR02150. IPP_isom_1. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BFZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQASKTHLD ELQLKRLEEM CIVIDKQDQI IGADTKKNCH LMENINKGLL
60 70 80 90 100
HRAFSVVLFN MKNELLVQQR ADAKYTFPGH FTDSCSSHPL YVPEELEEKD
110 120 130 140 150
ALGVRRAALR RLQAELGISQ DQISIKDIIF MTRKYHKCQS DAIWGEHEIG
160 170 180 190 200
YLLLVRKDLM LNPDTREVRR CCYMSQKDVQ ELLDREARGE EKITPWFRSM
210 220
VEDFLFSWWP HLEDVSSFVE PDKIYGL
Length:227
Mass (Da):26,665
Last modified:March 1, 2003 - v1
Checksum:iDACEA662BA4209A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201M → I in AAH99586 (PubMed:15489334).Curated
Sequence conflicti186 – 1861E → G in AAH99586 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263178 mRNA. Translation: AAP21674.1.
AK029364 mRNA. Translation: BAC26418.1.
AK081319 mRNA. Translation: BAC38194.1.
AC124732 Genomic DNA. No translation available.
BC099586 mRNA. Translation: AAH99586.1.
BC125310 mRNA. Translation: AAI25311.1.
BC138100 mRNA. Translation: AAI38101.1.
CCDSiCCDS26235.1.
RefSeqiNP_796171.1. NM_177197.4.
UniGeneiMm.313743.

Genome annotation databases

EnsembliENSMUST00000021573; ENSMUSP00000021573; ENSMUSG00000021148.
ENSMUST00000038598; ENSMUSP00000036621; ENSMUSG00000033520.
ENSMUST00000187196; ENSMUSP00000139705; ENSMUSG00000021148.
GeneIDi320581.
KEGGimmu:320581.
UCSCiuc007pko.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263178 mRNA. Translation: AAP21674.1.
AK029364 mRNA. Translation: BAC26418.1.
AK081319 mRNA. Translation: BAC38194.1.
AC124732 Genomic DNA. No translation available.
BC099586 mRNA. Translation: AAH99586.1.
BC125310 mRNA. Translation: AAI25311.1.
BC138100 mRNA. Translation: AAI38101.1.
CCDSiCCDS26235.1.
RefSeqiNP_796171.1. NM_177197.4.
UniGeneiMm.313743.

3D structure databases

ProteinModelPortaliQ8BFZ6.
SMRiQ8BFZ6. Positions 9-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036621.

Proteomic databases

PaxDbiQ8BFZ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021573; ENSMUSP00000021573; ENSMUSG00000021148.
ENSMUST00000038598; ENSMUSP00000036621; ENSMUSG00000033520.
ENSMUST00000187196; ENSMUSP00000139705; ENSMUSG00000021148.
GeneIDi320581.
KEGGimmu:320581.
UCSCiuc007pko.1. mouse.

Organism-specific databases

CTDi91734.
MGIiMGI:3704398. Gm9745.
MGI:2444315. Idi2.

Phylogenomic databases

eggNOGiKOG0142. Eukaryota.
COG1443. LUCA.
GeneTreeiENSGT00390000008527.
HOGENOMiHOG000274106.
HOVERGENiHBG002995.
InParanoidiQ8BFZ6.
KOiK01823.
OMAiPREVKSY.
OrthoDBiEOG7H4DVK.
PhylomeDBiQ8BFZ6.
TreeFamiTF300129.

Enzyme and pathway databases

UniPathwayiUPA00059; UER00104.
ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi397005.
PROiQ8BFZ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BFZ6.
GenevisibleiQ8BFZ6. MM.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR011876. IsopentenylPP_isomerase_typ1.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PANTHERiPTHR10885. PTHR10885. 1 hit.
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PIRSFiPIRSF018427. Isopntndiph_ism. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR02150. IPP_isom_1. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isopentenyl-diphosphate isomerases in human and mouse: evolutionary analysis of a mammalian gene duplication."
    Breitling R., Laubner D., Clizbe D., Adamski J., Krisans S.K.
    J. Mol. Evol. 57:282-291(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic head and Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiIDI2_MOUSE
AccessioniPrimary (citable) accession number: Q8BFZ6
Secondary accession number(s): Q4FZF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: March 1, 2003
Last modified: February 17, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.