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Protein

Transportin-1

Gene

Tnpo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (By similarity). In vitro, mediates nuclear import of SRP19 (By similarity) and H2A, H2B, H3 and H4 histones. Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Transportin-1
Alternative name(s):
Importin beta-2
Karyopherin beta-2
Gene namesi
Name:Tnpo1
Synonyms:Kpnb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2681523. Tnpo1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898Transportin-1PRO_0000120766Add
BLAST

Proteomic databases

EPDiQ8BFY9.
MaxQBiQ8BFY9.
PaxDbiQ8BFY9.
PeptideAtlasiQ8BFY9.
PRIDEiQ8BFY9.

PTM databases

iPTMnetiQ8BFY9.
PhosphoSiteiQ8BFY9.
SwissPalmiQ8BFY9.

Expressioni

Gene expression databases

BgeeiQ8BFY9.
CleanExiMM_TNPO1.
ExpressionAtlasiQ8BFY9. baseline and differential.
GenevisibleiQ8BFY9. MM.

Interactioni

Subunit structurei

Interacts with H2A, H2B, H3 and H4 histones (By similarity). Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with ADAR/ADAR1 (via DRBM 3 domain). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import. Interacts with SNAI2 (via zinc fingers) (By similarity).By similarity

Protein-protein interaction databases

BioGridi232018. 51 interactions.
IntActiQ8BFY9. 50 interactions.
STRINGi10090.ENSMUSP00000105028.

Structurei

3D structure databases

ProteinModelPortaliQ8BFY9.
SMRiQ8BFY9. Positions 11-897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati19 – 4628HEAT 1By similarityAdd
BLAST
Domaini41 – 10969Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati51 – 8939HEAT 2By similarityAdd
BLAST
Repeati98 – 13134HEAT 3By similarityAdd
BLAST
Repeati137 – 17438HEAT 4By similarityAdd
BLAST
Repeati181 – 21131HEAT 5By similarityAdd
BLAST
Repeati224 – 25128HEAT 6By similarityAdd
BLAST
Repeati263 – 29028HEAT 7By similarityAdd
BLAST
Repeati306 – 39792HEAT 8By similarityAdd
BLAST
Repeati405 – 43329HEAT 9By similarityAdd
BLAST
Repeati445 – 47228HEAT 10By similarityAdd
BLAST
Repeati486 – 51934HEAT 11By similarityAdd
BLAST
Repeati527 – 56034HEAT 12By similarityAdd
BLAST
Repeati568 – 60639HEAT 13By similarityAdd
BLAST
Repeati614 – 66552HEAT 14By similarityAdd
BLAST
Repeati676 – 70732HEAT 15By similarityAdd
BLAST
Repeati715 – 74834HEAT 16By similarityAdd
BLAST
Repeati756 – 79136HEAT 17By similarityAdd
BLAST
Repeati799 – 83234HEAT 18By similarityAdd
BLAST
Repeati841 – 87232HEAT 19By similarityAdd
BLAST
Repeati875 – 89521HEAT 20By similarityAdd
BLAST

Sequence similaritiesi

Contains 20 HEAT repeats.By similarity
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2023. Eukaryota.
ENOG410XPK2. LUCA.
GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
HOVERGENiHBG058963.
InParanoidiQ8BFY9.
KOiK18752.
OMAiWCTSLRH.
OrthoDBiEOG7XM2X0.
PhylomeDBiQ8BFY9.
TreeFamiTF300825.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BFY9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWDRQTKME YEWKPDEQGL QQILQLLKES QSPDTTIQRT VQQKLEQLNQ
60 70 80 90 100
YPDFNNYLIF VLTKLKSEDE PTRSLSGLIL KNNVKAHFQN FPNGVTDFIK
110 120 130 140 150
SECLNNIGDS SPLIRATVGI LITTIASKGE LQNWPDLLPK LCSLLDSEDY
160 170 180 190 200
NTCEGAFGAL QKICEDSAEI LDSDVLDRPL NIMIPKFLQF FKHSSPKIRS
210 220 230 240 250
HAVACVNQFI ISRTQALMLH IDSFIENLFA LAGDEEAEVR KNVCRALVML
260 270 280 290 300
LEVRMDRLLP HMHNIVEYML QRTQDQDENV ALEACEFWLT LAEQPICKDV
310 320 330 340 350
LVRHLPKLIP VLVNGMKYSD IDIILLKGDV EEDETIPDSE QDIRPRFHRS
360 370 380 390 400
RTVAQQHEED GIEEEDDDDD EIDDDDTISD WNLRKCSAAA LDVLANVYRD
410 420 430 440 450
ELLPHILPLL KELLFHHEWV VKESGILVLG AIAEGCMQGM IPYLPELIPH
460 470 480 490 500
LIQCLSDKKA LVRSITCWTL SRYAHWVVSQ PPDTYLKPLM TELLKRILDS
510 520 530 540 550
NKRVQEAACS AFATLEEEAC TELVPYLAYI LDTLVFAFSK YQHKNLLILY
560 570 580 590 600
DAIGTLADSV GHHLNKPEYI QMLMPPLIQK WNMLKDEDKD LFPLLECLSS
610 620 630 640 650
VATALQSGFL PYCEPVYQRC VNLVQKTLAQ AMLNNAQPEQ YEAPDKDFMI
660 670 680 690 700
VALDLLSGLA EGLGGNIEQL VARSNILTLM YQCMQDKMPE VRQSSFALLG
710 720 730 740 750
DLTKACFQHV KPCIADFMPI LGTNLNPEFI SVCNNATWAI GEISIQMGIE
760 770 780 790 800
MQPYIPMVLH QLVEIINRPN TPKTLLENTA ITIGRLGYVC PQEVAPMLQQ
810 820 830 840 850
FIRPWCTSLR NIRDNEEKDS AFRGICTMIS VNPSGVIQDF IFFCDAVASW
860 870 880 890
INPKDDLRDM FCKILHGFKN QVGDENWRRF SDQFPLPLKE RLAAFYGV
Length:898
Mass (Da):102,357
Last modified:September 22, 2009 - v2
Checksum:iE04FB7F8A67581E3
GO
Isoform 2 (identifier: Q8BFY9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MVWDRQTKM → M

Show »
Length:890
Mass (Da):101,312
Checksum:iDCA5612E4F94D57C
GO

Sequence cautioni

The sequence BAC26696.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC27029.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti389 – 3891A → S in BAC26696 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99MVWDRQTKM → M in isoform 2. 2 PublicationsVSP_038030

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029953 mRNA. Translation: BAC26696.1. Different initiation.
AK030580 mRNA. Translation: BAC27029.1. Different initiation.
AK045132 mRNA. Translation: BAC32236.1.
BC031571 mRNA. Translation: AAH31571.1.
BC055372 mRNA. Translation: AAH55372.1.
CCDSiCCDS26717.2. [Q8BFY9-1]
CCDS36760.1. [Q8BFY9-2]
RefSeqiNP_001041732.1. NM_001048267.1. [Q8BFY9-2]
NP_848831.2. NM_178716.3. [Q8BFY9-1]
XP_006517699.1. XM_006517636.2. [Q8BFY9-2]
XP_006517700.1. XM_006517637.2. [Q8BFY9-2]
UniGeneiMm.173286.

Genome annotation databases

EnsembliENSMUST00000109399; ENSMUSP00000105026; ENSMUSG00000009470. [Q8BFY9-2]
ENSMUST00000109401; ENSMUSP00000105028; ENSMUSG00000009470. [Q8BFY9-1]
GeneIDi238799.
KEGGimmu:238799.
UCSCiuc007rpd.1. mouse. [Q8BFY9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029953 mRNA. Translation: BAC26696.1. Different initiation.
AK030580 mRNA. Translation: BAC27029.1. Different initiation.
AK045132 mRNA. Translation: BAC32236.1.
BC031571 mRNA. Translation: AAH31571.1.
BC055372 mRNA. Translation: AAH55372.1.
CCDSiCCDS26717.2. [Q8BFY9-1]
CCDS36760.1. [Q8BFY9-2]
RefSeqiNP_001041732.1. NM_001048267.1. [Q8BFY9-2]
NP_848831.2. NM_178716.3. [Q8BFY9-1]
XP_006517699.1. XM_006517636.2. [Q8BFY9-2]
XP_006517700.1. XM_006517637.2. [Q8BFY9-2]
UniGeneiMm.173286.

3D structure databases

ProteinModelPortaliQ8BFY9.
SMRiQ8BFY9. Positions 11-897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232018. 51 interactions.
IntActiQ8BFY9. 50 interactions.
STRINGi10090.ENSMUSP00000105028.

PTM databases

iPTMnetiQ8BFY9.
PhosphoSiteiQ8BFY9.
SwissPalmiQ8BFY9.

Proteomic databases

EPDiQ8BFY9.
MaxQBiQ8BFY9.
PaxDbiQ8BFY9.
PeptideAtlasiQ8BFY9.
PRIDEiQ8BFY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109399; ENSMUSP00000105026; ENSMUSG00000009470. [Q8BFY9-2]
ENSMUST00000109401; ENSMUSP00000105028; ENSMUSG00000009470. [Q8BFY9-1]
GeneIDi238799.
KEGGimmu:238799.
UCSCiuc007rpd.1. mouse. [Q8BFY9-1]

Organism-specific databases

CTDi3842.
MGIiMGI:2681523. Tnpo1.

Phylogenomic databases

eggNOGiKOG2023. Eukaryota.
ENOG410XPK2. LUCA.
GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
HOVERGENiHBG058963.
InParanoidiQ8BFY9.
KOiK18752.
OMAiWCTSLRH.
OrthoDBiEOG7XM2X0.
PhylomeDBiQ8BFY9.
TreeFamiTF300825.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiTnpo1. mouse.
PROiQ8BFY9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BFY9.
CleanExiMM_TNPO1.
ExpressionAtlasiQ8BFY9. baseline and differential.
GenevisibleiQ8BFY9. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Pituitary and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary tumor and Mesenchymal cell.
  3. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTNPO1_MOUSE
AccessioniPrimary (citable) accession number: Q8BFY9
Secondary accession number(s): Q8C0S3, Q8K2E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: September 22, 2009
Last modified: July 6, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.