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Protein

Peflin

Gene

Pef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi118 – 1291PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi185 – 1962PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peflin
Alternative name(s):
PEF protein with a long N-terminal hydrophobic domain
Penta-EF hand domain-containing protein 1
Gene namesi
Name:Pef1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915148. Pef1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470461 – 275PeflinAdd BLAST275

Proteomic databases

EPDiQ8BFY6.
MaxQBiQ8BFY6.
PaxDbiQ8BFY6.
PeptideAtlasiQ8BFY6.
PRIDEiQ8BFY6.

PTM databases

PhosphoSitePlusiQ8BFY6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028779.
CleanExiMM_PEF1.
GenevisibleiQ8BFY6. MM.

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with PDCD6. Dissociates from PDCD6 in presence of Ca2+ (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030563.

Structurei

3D structure databases

ProteinModelPortaliQ8BFY6.
SMRiQ8BFY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 2919
Repeati31 – 3929
Repeati41 – 4939
Repeati50 – 59410
Repeati60 – 6859
Repeati76 – 8469
Repeati85 – 9177
Repeati92 – 10089
Domaini105 – 140EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini146 – 174EF-hand 2PROSITE-ProRule annotationAdd BLAST29
Domaini172 – 207EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini208 – 244EF-hand 4PROSITE-ProRule annotationAdd BLAST37
Domaini245 – 274EF-hand 5PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 1008 X 9 AA approximate tandem repeat of [AP]-P-G-G-P-Y-G-G-P-PAdd BLAST80
Regioni195 – 275Required for interaction with PDCD6By similarityAdd BLAST81

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000231983.
HOVERGENiHBG004492.
InParanoidiQ8BFY6.
OMAiFTNSNAM.
OrthoDBiEOG091G0ISY.
PhylomeDBiQ8BFY6.
TreeFamiTF314682.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BFY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASYPNGQSC PGAAGQVPGV PPGGYYPGPP HGGGQYGSGL PPGGGYGAPA
60 70 80 90 100
PGGPYGYPSA GGVPSGTPSG PYGGIPPGGP YGQLPPGGPY GTQPGHYGQG
110 120 130 140 150
GVPPNVDPEA YSWFQSVDAD HSGYISLKEL KQALVNSNWS SFNDETCLMM
160 170 180 190 200
INMFDKTKSG RIDVAGFSAL WKFLQQWRNL FQQYDRDRSG SISSTELQQA
210 220 230 240 250
LSQMGYNLSP QFTQLLVSRY CARSAIPAMQ LDCFIKVCTQ LQVLTEAFRE
260 270
KDTAVQGNIR LSFEDFVTMT ASRML
Length:275
Mass (Da):29,228
Last modified:March 1, 2003 - v1
Checksum:iDE4782B47ABEE9E2
GO

Sequence cautioni

The sequence BAB28735 differs from that shown. Reason: Frameshift at position 248.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148L → H in AAH19191 (PubMed:15489334).Curated1
Sequence conflicti218S → F in BAB28735 (PubMed:16141072).Curated1
Sequence conflicti257G → R in BAB25010 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007394 mRNA. Translation: BAB25010.1.
AK013238 mRNA. Translation: BAB28735.1. Frameshift.
AK046247 mRNA. Translation: BAC32654.1.
AK075978 mRNA. Translation: BAC36091.1.
BC019191 mRNA. Translation: AAH19191.1.
CCDSiCCDS18706.1.
RefSeqiNP_080717.2. NM_026441.4.
UniGeneiMm.20818.

Genome annotation databases

EnsembliENSMUST00000030563; ENSMUSP00000030563; ENSMUSG00000028779.
ENSMUST00000118199; ENSMUSP00000112638; ENSMUSG00000028779.
GeneIDi67898.
KEGGimmu:67898.
UCSCiuc008uyw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007394 mRNA. Translation: BAB25010.1.
AK013238 mRNA. Translation: BAB28735.1. Frameshift.
AK046247 mRNA. Translation: BAC32654.1.
AK075978 mRNA. Translation: BAC36091.1.
BC019191 mRNA. Translation: AAH19191.1.
CCDSiCCDS18706.1.
RefSeqiNP_080717.2. NM_026441.4.
UniGeneiMm.20818.

3D structure databases

ProteinModelPortaliQ8BFY6.
SMRiQ8BFY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030563.

PTM databases

PhosphoSitePlusiQ8BFY6.

Proteomic databases

EPDiQ8BFY6.
MaxQBiQ8BFY6.
PaxDbiQ8BFY6.
PeptideAtlasiQ8BFY6.
PRIDEiQ8BFY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030563; ENSMUSP00000030563; ENSMUSG00000028779.
ENSMUST00000118199; ENSMUSP00000112638; ENSMUSG00000028779.
GeneIDi67898.
KEGGimmu:67898.
UCSCiuc008uyw.1. mouse.

Organism-specific databases

CTDi553115.
MGIiMGI:1915148. Pef1.

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000231983.
HOVERGENiHBG004492.
InParanoidiQ8BFY6.
OMAiFTNSNAM.
OrthoDBiEOG091G0ISY.
PhylomeDBiQ8BFY6.
TreeFamiTF314682.

Miscellaneous databases

PROiQ8BFY6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028779.
CleanExiMM_PEF1.
GenevisibleiQ8BFY6. MM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BFY6
Secondary accession number(s): Q8VCT5, Q9CYW8, Q9D934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.