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Protein

Protein phosphatase 1 regulatory subunit 15B

Gene

Ppp1r15b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1.2 Publications

GO - Molecular functioni

  • protein serine/threonine phosphatase activity Source: MGI
  • translation regulator activity Source: MGI

GO - Biological processi

  • ER overload response Source: MGI
  • negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: ParkinsonsUK-UCL
  • peptidyl-serine dephosphorylation Source: ParkinsonsUK-UCL
  • regulation of translational initiation Source: MGI
  • response to endoplasmic reticulum stress Source: MGI
  • response to hydrogen peroxide Source: MGI
  • response to oxidative stress Source: MGI
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 15B
Alternative name(s):
Constitutive repressor of eIF2alpha phosphorylation
Short name:
CReP
Gene namesi
Name:Ppp1r15b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2444211. Ppp1r15b.

Subcellular locationi

GO - Cellular componenti

  • protein phosphatase type 1 complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian ratio, but exhibit severe growth retardation and impaired erythropoiesis. None survive the first day of postnatal life. PPP1R15A-PPP1R15B double-knockout embryos do not develop past the preimplantation period.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697Protein phosphatase 1 regulatory subunit 15BPRO_0000320521Add
BLAST

Proteomic databases

PaxDbiQ8BFW3.
PRIDEiQ8BFW3.

PTM databases

iPTMnetiQ8BFW3.
PhosphoSiteiQ8BFW3.

Expressioni

Gene expression databases

BgeeiQ8BFW3.
GenevisibleiQ8BFW3. MM.

Interactioni

Subunit structurei

Interacts with PP1. Part of a complex containing PPP1R15B, PP1 and NCK1/2.2 Publications

Protein-protein interaction databases

BioGridi224491. 1 interaction.
STRINGi10090.ENSMUSP00000057062.

Structurei

3D structure databases

ProteinModelPortaliQ8BFW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP1R15 family.Curated

Phylogenomic databases

eggNOGiENOG410IJ9C. Eukaryota.
ENOG4111TVA. LUCA.
GeneTreeiENSGT00510000048987.
HOGENOMiHOG000115677.
HOVERGENiHBG103996.
InParanoidiQ8BFW3.
KOiK17558.
OMAiWNSFYSV.
OrthoDBiEOG77DJ5H.
PhylomeDBiQ8BFW3.
TreeFamiTF105548.

Family and domain databases

InterProiIPR019523. Prot_Pase1_reg-su15A/B_C.
IPR019512. Prot_Pase1_reg-su15B_N.
[Graphical view]
PfamiPF10472. CReP_N. 1 hit.
PF10488. PP1c_bdg. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BFW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METGTHRARK RPGPRLGSWF RLPFLRRSHA CSSEFPPPSS RQNPGNSALP
60 70 80 90 100
ERRTRYWTKL LSQLLALLPS LFQKLLLWSQ LFGGLIPTRW LDFAASYSAL
110 120 130 140 150
RALRGREESA APTVQKSLSS LRLDSSEDLV VSSLDWLEEG LQWQCSSSDL
160 170 180 190 200
ELKLKAQERA LDSAAPTFLL EQQLWGVELL PSSLQAGLVS HRELDSSSSG
210 220 230 240 250
PLSVQSLGNF KVVSYLLNPS YLDYLPQLGL RCQSSAGGGQ FVGFRTLTPE
260 270 280 290 300
SCYLSEDGCH PQPLRAEMSA TAWRRCPPLS TEGLPEIHHL RMKRLEFLQA
310 320 330 340 350
NKGQELPTPD QDNGYHSLEE EHNLLRMDPQ HCTDNPAQAV SPAADRPEPT
360 370 380 390 400
EKKPELVIQE VSQSPQGSSL FCELPVEKEC EEDHTNATDL SDRGESLPVS
410 420 430 440 450
TRPVCSNKLI DYILGGAPSD LEASSDSESE DWGEEPEDDG FDSDGSLSES
460 470 480 490 500
DVEQDSEGLH LWNSFHSVDP YNPQNFTATI QTAARIAPRD PSDSGTSWSG
510 520 530 540 550
SCGVGSCQEG PLPETPDHSS GEEDDWEPSA DEAENLKLWN SFCHSEDPYN
560 570 580 590 600
LLNFKAPFQP SGKNWKGRQD SKASSEATVA FSGHHTLLSC KAQLLESQED
610 620 630 640 650
NCPGCGLGEA LAGERYTHIK RKKVTFLEEV TEYYISGDED RKGPWEEFAR
660 670 680 690
DGCRFQKRIQ ETEVAIGYCL AFEHREKMFN RLRIESKDLL LYSNVKK
Length:697
Mass (Da):77,712
Last modified:March 1, 2003 - v1
Checksum:iE439B12615F33737
GO
Isoform 2 (identifier: Q8BFW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-353: Missing.

Note: No experimental confirmation available.
Show »
Length:679
Mass (Da):75,838
Checksum:iAD4A96AB075AAAEE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei336 – 35318Missing in isoform 2. 1 PublicationVSP_031652Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049028 mRNA. Translation: BAC33517.1.
AK082957 mRNA. Translation: BAC38708.1.
AK086606 mRNA. Translation: BAC39701.1.
AK144678 mRNA. Translation: BAE26007.1.
AK146089 mRNA. Translation: BAE26892.1.
AK167565 mRNA. Translation: BAE39628.1.
AK170862 mRNA. Translation: BAE42079.1.
CCDSiCCDS15291.1. [Q8BFW3-1]
RefSeqiNP_598580.1. NM_133819.3. [Q8BFW3-1]
UniGeneiMm.293628.

Genome annotation databases

EnsembliENSMUST00000052529; ENSMUSP00000057062; ENSMUSG00000046062. [Q8BFW3-1]
GeneIDi108954.
KEGGimmu:108954.
UCSCiuc007cpt.2. mouse. [Q8BFW3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049028 mRNA. Translation: BAC33517.1.
AK082957 mRNA. Translation: BAC38708.1.
AK086606 mRNA. Translation: BAC39701.1.
AK144678 mRNA. Translation: BAE26007.1.
AK146089 mRNA. Translation: BAE26892.1.
AK167565 mRNA. Translation: BAE39628.1.
AK170862 mRNA. Translation: BAE42079.1.
CCDSiCCDS15291.1. [Q8BFW3-1]
RefSeqiNP_598580.1. NM_133819.3. [Q8BFW3-1]
UniGeneiMm.293628.

3D structure databases

ProteinModelPortaliQ8BFW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224491. 1 interaction.
STRINGi10090.ENSMUSP00000057062.

PTM databases

iPTMnetiQ8BFW3.
PhosphoSiteiQ8BFW3.

Proteomic databases

PaxDbiQ8BFW3.
PRIDEiQ8BFW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052529; ENSMUSP00000057062; ENSMUSG00000046062. [Q8BFW3-1]
GeneIDi108954.
KEGGimmu:108954.
UCSCiuc007cpt.2. mouse. [Q8BFW3-1]

Organism-specific databases

CTDi84919.
MGIiMGI:2444211. Ppp1r15b.

Phylogenomic databases

eggNOGiENOG410IJ9C. Eukaryota.
ENOG4111TVA. LUCA.
GeneTreeiENSGT00510000048987.
HOGENOMiHOG000115677.
HOVERGENiHBG103996.
InParanoidiQ8BFW3.
KOiK17558.
OMAiWNSFYSV.
OrthoDBiEOG77DJ5H.
PhylomeDBiQ8BFW3.
TreeFamiTF105548.

Miscellaneous databases

NextBioi361512.
PROiQ8BFW3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BFW3.
GenevisibleiQ8BFW3. MM.

Family and domain databases

InterProiIPR019523. Prot_Pase1_reg-su15A/B_C.
IPR019512. Prot_Pase1_reg-su15B_N.
[Graphical view]
PfamiPF10472. CReP_N. 1 hit.
PF10488. PP1c_bdg. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Cerebellum, Head, Liver, Lung, Placenta and Spinal cord.
  2. "Inhibition of a constitutive translation initiation factor 2alpha phosphatase, CReP, promotes survival of stressed cells."
    Jousse C., Oyadomari S., Novoa I., Lu P., Zhang Y., Harding H.P., Ron D.
    J. Cell Biol. 163:767-775(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PP1.
  3. "Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress."
    Latreille M., Larose L.
    J. Biol. Chem. 281:26633-26644(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN COMPLEX WITH PP1 AND NCK1.
  4. "Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2alpha) dephosphorylation in mammalian development."
    Harding H.P., Zhang Y., Scheuner D., Chen J.J., Kaufman R.J., Ron D.
    Proc. Natl. Acad. Sci. U.S.A. 106:1832-1837(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPR15B_MOUSE
AccessioniPrimary (citable) accession number: Q8BFW3
Secondary accession number(s): Q3TC78, Q8C390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.