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Protein

Vesicular glutamate transporter 3

Gene

Slc17a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.4 Publications

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: MGI
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hearing, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-428643. Organic anion transporters.

Protein family/group databases

TCDBi2.A.1.14.23. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 3
Short name:
VGluT3
Alternative name(s):
Solute carrier family 17 member 8
Gene namesi
Name:Slc17a8
Synonyms:Vglut3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:3039629. Slc17a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8989CytoplasmicSequence analysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Topological domaini111 – 14333VesicularSequence analysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Topological domaini165 – 1662CytoplasmicSequence analysis
Transmembranei167 – 18721HelicalSequence analysisAdd
BLAST
Topological domaini188 – 1958VesicularSequence analysis
Transmembranei196 – 21621HelicalSequence analysisAdd
BLAST
Topological domaini217 – 23418CytoplasmicSequence analysisAdd
BLAST
Transmembranei235 – 25521HelicalSequence analysisAdd
BLAST
Topological domaini256 – 2627VesicularSequence analysis
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Topological domaini284 – 32744CytoplasmicSequence analysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence analysisAdd
BLAST
Topological domaini349 – 36618VesicularSequence analysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence analysisAdd
BLAST
Topological domaini388 – 40316CytoplasmicSequence analysisAdd
BLAST
Transmembranei404 – 42421HelicalSequence analysisAdd
BLAST
Topological domaini425 – 4262VesicularSequence analysis
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST
Topological domaini448 – 46013CytoplasmicSequence analysisAdd
BLAST
Transmembranei461 – 48121HelicalSequence analysisAdd
BLAST
Topological domaini482 – 49413VesicularSequence analysisAdd
BLAST
Transmembranei495 – 51521HelicalSequence analysisAdd
BLAST
Topological domaini516 – 59883CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice are hyperactive and suffer from intermittent, spontaneous cortical seizures. They exhibit reduced cholinergic transmission in the ventral portion of the striatum and defective acetylcholine release. They are hypersensitive to cocaine and less prone to haloperidol-induced catalepsy.3 Publications

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Vesicular glutamate transporter 3PRO_0000331615Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BFU8.
PaxDbiQ8BFU8.
PRIDEiQ8BFU8.

Expressioni

Tissue specificityi

Expressed in restricted areas of the brain. Highest expression is found in the neurons of the basal forebrain, the hippocampal formation, and the majority of the neurons of the mesencephalic raphe nuclei. Expressed in inner hair cells of the ear.2 Publications

Developmental stagei

Expression peaks at P7 in the brain. Expressed in inner hair cells from E19 onwards.2 Publications

Gene expression databases

BgeeiQ8BFU8.
ExpressionAtlasiQ8BFU8. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020102.

Structurei

3D structure databases

ProteinModelPortaliQ8BFU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 8319Cys-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ8BFU8.
KOiK12302.
OMAiCGIIGED.
OrthoDBiEOG789C9Z.
PhylomeDBiQ8BFU8.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BFU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFKAFDTFK EKILKPGKEG VKNAVGDSLG ILQRKIDGTN EEEDAIELNE
60 70 80 90 100
EGRPVQTSRA HRPVCDCSCC GIPKRYICDC SCCGIPKRYI IAVMSGLGFC
110 120 130 140 150
ISFGIRCNLG VAIVEMVNNS TVYVDGKPEI QTAQFNWDPE TVGLIHGSFF
160 170 180 190 200
WGYIVTQIPG GFISNKFAAS RVFGAAIFLT STLNMFIPSA ARVHYGCVMG
210 220 230 240 250
VRILQGLVEG VTYPACHGMW SKWAPPLERS RLATTSFCGS YAGAVVAMPL
260 270 280 290 300
AGVLVQYIGW ASVFYIYGMF GIIWYMFWLL QAYECPAAHP TISNAERTYI
310 320 330 340 350
ETSIGEGANL ASLSKFNTPW RRFFTSLPVY AIIVANFCRS WTFYLLLISQ
360 370 380 390 400
PAYFEEVFGF AISKVGLLSA VPHMVMTIVV PIGGQLADYL RSRKILTTTA
410 420 430 440 450
VRKIMNCGGF GMEATLLLVV GFSHTKGVAI SFLVLAVGFS GFAISGFNVN
460 470 480 490 500
HLDIAPRYAS ILMGISNGVG TLSGMVCPLI VGAMTKHKTR EEWQNVFLIA
510 520 530 540 550
ALVHYSGVIF YGVFASGEKQ DWADPENLSE DKCGIIDQDE LAEETELNHE
560 570 580 590 600
TFVSPRKKMS YGATTQNCEV QKTEWRQQRE SAFDGEEPLS YQAEGDFSET

S
Length:601
Mass (Da):66,148
Last modified:March 1, 2003 - v1
Checksum:iEBF6FC6E0C64961B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF510321 mRNA. Translation: AAN74643.1.
BC042593 mRNA. Translation: AAH42593.1.
CCDSiCCDS24117.1.
RefSeqiNP_892004.1. NM_182959.3.
UniGeneiMm.233921.

Genome annotation databases

EnsembliENSMUST00000020102; ENSMUSP00000020102; ENSMUSG00000019935.
GeneIDi216227.
KEGGimmu:216227.
UCSCiuc007gsj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF510321 mRNA. Translation: AAN74643.1.
BC042593 mRNA. Translation: AAH42593.1.
CCDSiCCDS24117.1.
RefSeqiNP_892004.1. NM_182959.3.
UniGeneiMm.233921.

3D structure databases

ProteinModelPortaliQ8BFU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020102.

Protein family/group databases

TCDBi2.A.1.14.23. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiQ8BFU8.
PaxDbiQ8BFU8.
PRIDEiQ8BFU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020102; ENSMUSP00000020102; ENSMUSG00000019935.
GeneIDi216227.
KEGGimmu:216227.
UCSCiuc007gsj.1. mouse.

Organism-specific databases

CTDi246213.
MGIiMGI:3039629. Slc17a8.

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ8BFU8.
KOiK12302.
OMAiCGIIGED.
OrthoDBiEOG789C9Z.
PhylomeDBiQ8BFU8.
TreeFamiTF313535.

Enzyme and pathway databases

ReactomeiR-MMU-428643. Organic anion transporters.

Miscellaneous databases

PROiQ8BFU8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BFU8.
ExpressionAtlasiQ8BFU8. baseline and differential.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons."
    Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D.
    J. Biol. Chem. 277:50734-50748(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Impairment of SLC17A8 encoding vesicular glutamate transporter-3, VGLUT3, underlies nonsyndromic deafness DFNA25 and inner hair cell dysfunction in null mice."
    Ruel J., Emery S., Nouvian R., Bersot T., Amilhon B., Van Rybroek J.M., Rebillard G., Lenoir M., Eybalin M., Delprat B., Sivakumaran T.A., Giros B., El Mestikawy S., Moser T., Smith R.J.H., Lesperance M.M., Puel J.-L.
    Am. J. Hum. Genet. 83:278-292(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
    Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
    Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3."
    Seal R.P., Akil O., Yi E., Weber C.M., Grant L., Yoo J., Clause A., Kandler K., Noebels J.L., Glowatzki E., Lustig L.R., Edwards R.H.
    Neuron 57:263-275(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiVGLU3_MOUSE
AccessioniPrimary (citable) accession number: Q8BFU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice defective in Slc17a8 are profoundly deaf owing to the absence of glutamate release from hair cells at the first synapse in the auditory pathway. They lack auditory-nerve responses to acoustic stimuli, although auditory brainstem responses could be elicited by electrical stimuli.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.