Vesicular glutamate transporter 3 - Mus musculus (Mouse)

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Q8BFU8 (VGLU3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 69. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vesicular glutamate transporter 3
Short name=VGluT3

Alternative name(s):
Solute carrier family 17 member 8

Gene names

Name:	Slc17a8
Synonyms:	Vglut3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.1 Ref.4 Ref.5 Ref.6
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane By similarity. Membrane; Multi-pass membrane protein Potential. Cell junction › synapse › synaptosome By similarity Ref.1.
Tissue specificity	Expressed in restricted areas of the brain. Highest expression is found in the neurons of the basal forebrain, the hippocampal formation, and the majority of the neurons of the mesencephalic raphe nuclei. Expressed in inner hair cells of the ear. Ref.1 Ref.6
Developmental stage	Expression peaks at P7 in the brain. Expressed in inner hair cells from E19 onwards. Ref.1 Ref.6
Disruption phenotype	Mice are hyperactive and suffer from intermittent, spontaneous cortical seizures. They exhibit reduced cholinergic transmission in the ventral portion of the striatum and defective acetylcholine release. They are hypersensitive to cocaine and less prone to haloperidol-induced catalepsy. Ref.3 Ref.4 Ref.6
Miscellaneous	Mice defective in Slc17a8 are profoundly deaf owing to the absence of glutamate release from hair cells at the first synapse in the auditory pathway. They lack auditory-nerve responses to acoustic stimuli, although auditory brainstem responses could be elicited by electrical stimuli.
Sequence similarities	Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
Biological process	Hearing Ion transport Neurotransmitter transport Sodium transport Symport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Disease	Deafness
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW synaptic vesicle membrane Inferred from direct assay Ref.1. Source: MGI synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-glutamate transmembrane transporter activity Inferred from direct assay Ref.1. Source: MGI symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 601

601

Vesicular glutamate transporter 3

PRO_0000331615

Regions

Topological domain

1 – 89

Cytoplasmic Potential

Transmembrane

90 – 110

Helical; Potential

Topological domain

111 – 143

Vesicular Potential

Transmembrane

144 – 164

Helical; Potential

Topological domain

165 – 166

Cytoplasmic Potential

Transmembrane

167 – 187

Helical; Potential

Topological domain

188 – 195

Vesicular Potential

Transmembrane

196 – 216

Helical; Potential

Topological domain

217 – 234

Cytoplasmic Potential

Transmembrane

235 – 255

Helical; Potential

Topological domain

256 – 262

Vesicular Potential

Transmembrane

263 – 283

Helical; Potential

Topological domain

284 – 327

Cytoplasmic Potential

Transmembrane

328 – 348

Helical; Potential

Topological domain

349 – 366

Vesicular Potential

Transmembrane

367 – 387

Helical; Potential

Topological domain

388 – 403

Cytoplasmic Potential

Transmembrane

404 – 424

Helical; Potential

Topological domain

425 – 426

Vesicular Potential

Transmembrane

427 – 447

Helical; Potential

Topological domain

448 – 460

Cytoplasmic Potential

Transmembrane

461 – 481

Helical; Potential

Topological domain

482 – 494

Vesicular Potential

Transmembrane

495 – 515

Helical; Potential

Topological domain

516 – 598

Cytoplasmic Potential

Compositional bias

65 – 83

Cys-rich

Amino acid modifications

Glycosylation

119

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8BFU8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: EBF6FC6E0C64961B
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FASTA

601

66,148

        10         20         30         40         50         60 
MPFKAFDTFK EKILKPGKEG VKNAVGDSLG ILQRKIDGTN EEEDAIELNE EGRPVQTSRA 

        70         80         90        100        110        120 
HRPVCDCSCC GIPKRYICDC SCCGIPKRYI IAVMSGLGFC ISFGIRCNLG VAIVEMVNNS 

       130        140        150        160        170        180 
TVYVDGKPEI QTAQFNWDPE TVGLIHGSFF WGYIVTQIPG GFISNKFAAS RVFGAAIFLT 

       190        200        210        220        230        240 
STLNMFIPSA ARVHYGCVMG VRILQGLVEG VTYPACHGMW SKWAPPLERS RLATTSFCGS 

       250        260        270        280        290        300 
YAGAVVAMPL AGVLVQYIGW ASVFYIYGMF GIIWYMFWLL QAYECPAAHP TISNAERTYI 

       310        320        330        340        350        360 
ETSIGEGANL ASLSKFNTPW RRFFTSLPVY AIIVANFCRS WTFYLLLISQ PAYFEEVFGF 

       370        380        390        400        410        420 
AISKVGLLSA VPHMVMTIVV PIGGQLADYL RSRKILTTTA VRKIMNCGGF GMEATLLLVV 

       430        440        450        460        470        480 
GFSHTKGVAI SFLVLAVGFS GFAISGFNVN HLDIAPRYAS ILMGISNGVG TLSGMVCPLI 

       490        500        510        520        530        540 
VGAMTKHKTR EEWQNVFLIA ALVHYSGVIF YGVFASGEKQ DWADPENLSE DKCGIIDQDE 

       550        560        570        580        590        600 
LAEETELNHE TFVSPRKKMS YGATTQNCEV QKTEWRQQRE SAFDGEEPLS YQAEGDFSET 


S

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D. J. Biol. Chem. 277:50734-50748(2002) [PubMed: 12384506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: C57BL/6. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[3]	"Impairment of SLC17A8 encoding vesicular glutamate transporter-3, VGLUT3, underlies nonsyndromic deafness DFNA25 and inner hair cell dysfunction in null mice." Ruel J., Emery S., Nouvian R., Bersot T., Amilhon B., Van Rybroek J.M., Rebillard G., Lenoir M., Eybalin M., Delprat B., Sivakumaran T.A., Giros B., El Mestikawy S., Moser T., Smith R.J.H., Lesperance M.M., Puel J.-L. Am. J. Hum. Genet. 83:278-292(2008) [PubMed: 18674745] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[4]	"The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone." Gras C., Amilhon B., Lepicard E.M., Poirel O., Vinatier J., Herbin M., Dumas S., Tzavara E.T., Wade M.R., Nomikos G.G., Hanoun N., Saurini F., Kemel M.-L., Gasnier B., Giros B., Mestikawy S.E. Nat. Neurosci. 11:292-300(2008) [PubMed: 18278042] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]	"Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging." Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D. Neurochem. Res. 33:238-247(2008) [PubMed: 18080752] [Abstract] Cited for: FUNCTION.
[6]	"Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3." Seal R.P., Akil O., Yi E., Weber C.M., Grant L., Yoo J., Clause A., Kandler K., Noebels J.L., Glowatzki E., Lustig L.R., Edwards R.H. Neuron 57:263-275(2008) [PubMed: 18215623] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF510321 mRNA. Translation: AAN74643.1. BC042593 mRNA. Translation: AAH42593.1.
IPI	IPI00221470.
RefSeq	NP_892004.1. NM_182959.3.
UniGene	Mm.233921.
3D structure databases
ProteinModelPortal	Q8BFU8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BFU8.
Protein family/group databases
TCDB	2.A.1.14.23. major facilitator superfamily (MFS).
Proteomic databases
PRIDE	Q8BFU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020102; ENSMUSP00000020102; ENSMUSG00000019935.
GeneID	216227.
KEGG	mmu:216227.
UCSC	uc007gsk.2. mouse.
Organism-specific databases
CTD	246213.
MGI	MGI:3039629. Slc17a8.
Phylogenomic databases
GeneTree	ENSGT00600000084103.
HOGENOM	HBG561587.
HOVERGEN	HBG008834.
InParanoid	Q8BFU8.
OMA	YMFWLLQ.
OrthoDB	EOG4XWFXC.
PhylomeDB	Q8BFU8.
Gene expression databases
ArrayExpress	Q8BFU8.
Bgee	Q8BFU8.
Genevestigator	Q8BFU8.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
KO	K12302.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	375084.
SOURCE	Search...

Entry information

Entry name

VGLU3_MOUSE

Accession

Primary (citable) accession number: Q8BFU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents