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Protein

Vesicular glutamate transporter 3

Gene

Slc17a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.4 Publications

Miscellaneous

Mice defective in Slc17a8 are profoundly deaf owing to the absence of glutamate release from hair cells at the first synapse in the auditory pathway. They lack auditory-nerve responses to acoustic stimuli, although auditory brainstem responses could be elicited by electrical stimuli.

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: MGI
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processHearing, Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-428643 Organic anion transporters

Protein family/group databases

TCDBi2.A.1.14.23 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 3
Short name:
VGluT3
Alternative name(s):
Solute carrier family 17 member 8
Gene namesi
Name:Slc17a8
Synonyms:Vglut3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:3039629 Slc17a8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 89CytoplasmicSequence analysisAdd BLAST89
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 143VesicularSequence analysisAdd BLAST33
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 166CytoplasmicSequence analysis2
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 195VesicularSequence analysis8
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 234CytoplasmicSequence analysisAdd BLAST18
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Topological domaini256 – 262VesicularSequence analysis7
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 327CytoplasmicSequence analysisAdd BLAST44
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 366VesicularSequence analysisAdd BLAST18
Transmembranei367 – 387HelicalSequence analysisAdd BLAST21
Topological domaini388 – 403CytoplasmicSequence analysisAdd BLAST16
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Topological domaini425 – 426VesicularSequence analysis2
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Topological domaini448 – 460CytoplasmicSequence analysisAdd BLAST13
Transmembranei461 – 481HelicalSequence analysisAdd BLAST21
Topological domaini482 – 494VesicularSequence analysisAdd BLAST13
Transmembranei495 – 515HelicalSequence analysisAdd BLAST21
Topological domaini516 – 598CytoplasmicSequence analysisAdd BLAST83

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice are hyperactive and suffer from intermittent, spontaneous cortical seizures. They exhibit reduced cholinergic transmission in the ventral portion of the striatum and defective acetylcholine release. They are hypersensitive to cocaine and less prone to haloperidol-induced catalepsy.3 Publications

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003316151 – 601Vesicular glutamate transporter 3Add BLAST601

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BFU8
PRIDEiQ8BFU8

PTM databases

SwissPalmiQ8BFU8

Expressioni

Tissue specificityi

Expressed in restricted areas of the brain. Highest expression is found in the neurons of the basal forebrain, the hippocampal formation, and the majority of the neurons of the mesencephalic raphe nuclei. Expressed in inner hair cells of the ear.2 Publications

Developmental stagei

Expression peaks at P7 in the brain. Expressed in inner hair cells from E19 onwards.2 Publications

Gene expression databases

BgeeiENSMUSG00000019935
ExpressionAtlasiQ8BFU8 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020102

Structurei

3D structure databases

ProteinModelPortaliQ8BFU8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 83Cys-richAdd BLAST19

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532 Eukaryota
ENOG410XPWC LUCA
GeneTreeiENSGT00760000119079
HOGENOMiHOG000230812
HOVERGENiHBG008834
InParanoidiQ8BFU8
KOiK12302
OMAiNNSTVYV
OrthoDBiEOG091G06DG
PhylomeDBiQ8BFU8
TreeFamiTF313535

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BFU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFKAFDTFK EKILKPGKEG VKNAVGDSLG ILQRKIDGTN EEEDAIELNE
60 70 80 90 100
EGRPVQTSRA HRPVCDCSCC GIPKRYICDC SCCGIPKRYI IAVMSGLGFC
110 120 130 140 150
ISFGIRCNLG VAIVEMVNNS TVYVDGKPEI QTAQFNWDPE TVGLIHGSFF
160 170 180 190 200
WGYIVTQIPG GFISNKFAAS RVFGAAIFLT STLNMFIPSA ARVHYGCVMG
210 220 230 240 250
VRILQGLVEG VTYPACHGMW SKWAPPLERS RLATTSFCGS YAGAVVAMPL
260 270 280 290 300
AGVLVQYIGW ASVFYIYGMF GIIWYMFWLL QAYECPAAHP TISNAERTYI
310 320 330 340 350
ETSIGEGANL ASLSKFNTPW RRFFTSLPVY AIIVANFCRS WTFYLLLISQ
360 370 380 390 400
PAYFEEVFGF AISKVGLLSA VPHMVMTIVV PIGGQLADYL RSRKILTTTA
410 420 430 440 450
VRKIMNCGGF GMEATLLLVV GFSHTKGVAI SFLVLAVGFS GFAISGFNVN
460 470 480 490 500
HLDIAPRYAS ILMGISNGVG TLSGMVCPLI VGAMTKHKTR EEWQNVFLIA
510 520 530 540 550
ALVHYSGVIF YGVFASGEKQ DWADPENLSE DKCGIIDQDE LAEETELNHE
560 570 580 590 600
TFVSPRKKMS YGATTQNCEV QKTEWRQQRE SAFDGEEPLS YQAEGDFSET

S
Length:601
Mass (Da):66,148
Last modified:March 1, 2003 - v1
Checksum:iEBF6FC6E0C64961B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF510321 mRNA Translation: AAN74643.1
BC042593 mRNA Translation: AAH42593.1
CCDSiCCDS24117.1
RefSeqiNP_892004.1, NM_182959.3
UniGeneiMm.233921

Genome annotation databases

EnsembliENSMUST00000020102; ENSMUSP00000020102; ENSMUSG00000019935
GeneIDi216227
KEGGimmu:216227
UCSCiuc007gsj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiVGLU3_MOUSE
AccessioniPrimary (citable) accession number: Q8BFU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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