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Q8BFS3 (REL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Relaxin-3
Alternative name(s):
Insulin-like peptide INSL7
Short name=Insulin-like peptide 7
Prorelaxin R3

Cleaved into the following 2 chains:

  1. Relaxin-3 B chain
  2. Relaxin-3 A chain
Gene names
Name:Rln3
Synonyms:Insl7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length140 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 and relaxin-3 receptor-2 By similarity.

Subunit structure

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location

Secreted.

Tissue specificity

Highly abundant expression is detected in neurons within the ventomedial dorsal tegmental nucleus and the laterally central gray alpha of the pons. Also detected at much lower levels within the hippocampus. Ref.1

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled receptor binding

Inferred from mutant phenotype PubMed 14522968. Source: RGD

hormone activity

Traceable author statement PubMed 14522968. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Peptide24 – 5027Relaxin-3 B chain By similarity
PRO_0000016094
Propeptide53 – 11664Connecting peptide By similarity
PRO_0000016095
Peptide117 – 14024Relaxin-3 A chain By similarity
PRO_0000016096

Amino acid modifications

Disulfide bond33 ↔ 127Interchain (between B and A chains) By similarity
Disulfide bond45 ↔ 140Interchain (between B and A chains) By similarity
Disulfide bond126 ↔ 131 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8BFS3 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F4B6979756122EDA

FASTA14014,922
        10         20         30         40         50         60 
MATRGLLLAS WALLGALVLQ AEARPAPYGV KLCGREFIRA VIFTCGGSRW RRADILAHDP 

        70         80         90        100        110        120 
LGEFFADGEA NTDHLASELD EAVGSSEWLA LTKSPQVFYG GRSSWQGSPG VVRGSRDVLA 

       130        140 
GLSSSCCEWG CSKSQISSLC 

« Hide

References

[1]"Restricted, but abundant, expression of the novel rat gene-3 (R3) relaxin in the dorsal tegmental region of brain."
Burazin T.C.D., Bathgate R.A.D., Macris M., Layfield S., Gundlach A.L., Tregear G.W.
J. Neurochem. 82:1553-1557(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Production of recombinant human relaxin 3 in AtT20 cells."
Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N., Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M.
Regul. Pept. 113:79-84(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY112741 mRNA. Translation: AAM56033.1.
AB076564 mRNA. Translation: BAC53759.1.
RefSeqNP_733767.1. NM_170667.2.
UniGeneRn.45123.

3D structure databases

ProteinModelPortalQ8BFS3.
SMRQ8BFS3. Positions 24-50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000007775.

Proteomic databases

PRIDEQ8BFS3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000007775; ENSRNOP00000007775; ENSRNOG00000005911.
GeneID266997.
KEGGrno:266997.

Organism-specific databases

CTD117579.
RGD628745. Rln3.

Phylogenomic databases

eggNOGNOG45013.
GeneTreeENSGT00510000048203.
HOGENOMHOG000111888.
HOVERGENHBG045048.
InParanoidQ8BFS3.
KOK05255.
OMARPSWQGT.
OrthoDBEOG7H1JNG.
PhylomeDBQ8BFS3.
TreeFamTF333404.

Gene expression databases

GenevestigatorQ8BFS3.

Family and domain databases

InterProIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 2 hits.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624753.
PROQ8BFS3.

Entry information

Entry nameREL3_RAT
AccessionPrimary (citable) accession number: Q8BFS3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families