Q8BFS3 (REL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Relaxin-3

Alternative name(s):
Insulin-like peptide INSL7
Short name=Insulin-like peptide 7
Prorelaxin R3

Cleaved into the following 2 chains:

1. Relaxin-3 B chain
2. Relaxin-3 A chain

Gene names

Name:	Rln3
Synonyms:	Insl7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 and relaxin-3 receptor-2 By similarity.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Tissue specificity	Highly abundant expression is detected in neurons within the ventomedial dorsal tegmental nucleus and the laterally central gray alpha of the pons. Also detected at much lower levels within the hippocampus. Ref.1
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Inferred from mutant phenotype PubMed 14522968. Source: RGD hormone activity Traceable author statement PubMed 14522968. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Peptide	24 – 50	27	Relaxin-3 B chain By similarity		PRO_0000016094
	Propeptide	53 – 116	64	Connecting peptide By similarity		PRO_0000016095
	Peptide	117 – 140	24	Relaxin-3 A chain By similarity		PRO_0000016096
Amino acid modifications
	Disulfide bond	33 ↔ 127		Interchain (between B and A chains) By similarity
	Disulfide bond	45 ↔ 140		Interchain (between B and A chains) By similarity
	Disulfide bond	126 ↔ 131		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8BFS3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F4B6979756122EDA	FASTA	140	14,922
	10 20 30 40 50 60 MATRGLLLAS WALLGALVLQ AEARPAPYGV KLCGREFIRA VIFTCGGSRW RRADILAHDP 70 80 90 100 110 120 LGEFFADGEA NTDHLASELD EAVGSSEWLA LTKSPQVFYG GRSSWQGSPG VVRGSRDVLA 130 140 GLSSSCCEWG CSKSQISSLC

References

[1]	"Restricted, but abundant, expression of the novel rat gene-3 (R3) relaxin in the dorsal tegmental region of brain." Burazin T.C.D., Bathgate R.A.D., Macris M., Layfield S., Gundlach A.L., Tregear G.W. J. Neurochem. 82:1553-1557(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Production of recombinant human relaxin 3 in AtT20 cells." Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N., Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M. Regul. Pept. 113:79-84(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY112741 mRNA. Translation: AAM56033.1. AB076564 mRNA. Translation: BAC53759.1.
IPI	IPI00188017.
RefSeq	NP_733767.1. NM_170667.2.
UniGene	Rn.45123.
3D structure databases
ProteinModelPortal	Q8BFS3.
SMR	Q8BFS3. Positions 24-50.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007775.
Proteomic databases
PRIDE	Q8BFS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007775; ENSRNOP00000007775; ENSRNOG00000005911.
GeneID	266997.
KEGG	rno:266997.
Organism-specific databases
CTD	117579.
RGD	628745. Rln3.
Phylogenomic databases
eggNOG	NOG45013.
GeneTree	ENSGT00510000048203.
HOGENOM	HOG000111888.
HOVERGEN	HBG045048.
InParanoid	Q8BFS3.
KO	K05255.
OMA	RPSWQGT.
OrthoDB	EOG4VHK7X.
Gene expression databases
Genevestigator	Q8BFS3.
Family and domain databases
InterPro	IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624753.

Entry information

Entry name

REL3_RAT

Accession

Primary (citable) accession number: Q8BFS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program