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Q8BFR5

- EFTU_MOUSE

UniProt

Q8BFR5 - EFTU_MOUSE

Protein

Elongation factor Tu, mitochondrial

Gene

Tufm

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi64 – 718GTPBy similarity
    Nucleotide bindingi126 – 1305GTPBy similarity
    Nucleotide bindingi181 – 1844GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. translation elongation factor activity Source: UniProtKB

    GO - Biological processi

    1. translational elongation Source: UniProtKB

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor Tu, mitochondrial
    Gene namesi
    Name:Tufm
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1923686. Tufm.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: MGI
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4343MitochondrionBy similarityAdd
    BLAST
    Chaini44 – 452409Elongation factor Tu, mitochondrialPRO_0000007463Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei79 – 791N6-acetyllysineBy similarity
    Modified residuei88 – 881N6-acetyllysineBy similarity
    Modified residuei256 – 2561N6-acetyllysineBy similarity
    Modified residuei418 – 4181N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8BFR5.
    PaxDbiQ8BFR5.
    PRIDEiQ8BFR5.

    2D gel databases

    REPRODUCTION-2DPAGEQ6P919.
    Q8BFR5.

    PTM databases

    PhosphoSiteiQ8BFR5.

    Expressioni

    Gene expression databases

    BgeeiQ8BFR5.
    CleanExiMM_TUFM.
    GenevestigatoriQ8BFR5.

    Interactioni

    Protein-protein interaction databases

    BioGridi231459. 4 interactions.
    IntActiQ8BFR5. 8 interactions.
    MINTiMINT-1860932.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BFR5.
    SMRiQ8BFR5. Positions 55-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 251197tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 718G1PROSITE-ProRule annotation
    Regioni105 – 1095G2PROSITE-ProRule annotation
    Regioni126 – 1294G3PROSITE-ProRule annotation
    Regioni181 – 1844G4PROSITE-ProRule annotation
    Regioni219 – 2213G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0050.
    GeneTreeiENSGT00550000074682.
    HOGENOMiHOG000229290.
    HOVERGENiHBG001535.
    InParanoidiQ8BFR5.
    KOiK02358.
    OMAiGTEMCMP.
    OrthoDBiEOG73BVCN.
    PhylomeDBiQ8BFR5.
    TreeFamiTF300432.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BFR5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAATLLRAT PRFSGLCASP TPFLQGRLRP LKAPASPFLC RGLAVEAKKT    50
    YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE 100
    ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV 150
    VAANDGPMPQ TREHLLLAKQ IGVEHVVVYV NKADAVQDSE MVELVELEIR 200
    ELLTEFGYKG EETPVIVGSA LCALEQRDPE LGVKSVQKLL DAVDTYIPVP 250
    TRDLDKPFLL PVESVYSIPG RGTVVTGTLE RGILKKGDEC ELLGHNKNIR 300
    TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIQPHQ 350
    KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRVI LPPGKELAMP 400
    GEDLKLSLIL RQPMILEKGQ RFTLRDGNKT IGTGLVTDVP AMTEEDKNIK 450
    WS 452
    Length:452
    Mass (Da):49,508
    Last modified:March 1, 2003 - v1
    Checksum:iF8687A05FFCB337D
    GO
    Isoform 2 (identifier: Q8BFR5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         357-452: YILSKEEGGR...TEEDKNIKWS → RAPVLSGFPC...TCHAWRGLEA

    Note: No experimental confirmation available.

    Show »
    Length:435
    Mass (Da):47,222
    Checksum:i6821088F0D8A5145
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei357 – 45296YILSK…NIKWS → RAPVLSGFPCLEAGLVAKPF HPYCFSPLGLYPQQGGRWPP QTLCISFHARHVLPDLGHGL SSHLASREGTCHAWRGLEA in isoform 2. 1 PublicationVSP_013942Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK075857 mRNA. Translation: BAC36008.1.
    AK084724 mRNA. Translation: BAC39263.1.
    AK153135 mRNA. Translation: BAE31747.1.
    AK152858 mRNA. Translation: BAE31551.1.
    BC060959 mRNA. Translation: AAH60959.1.
    BC100596 mRNA. Translation: AAI00597.1.
    CCDSiCCDS21830.1. [Q8BFR5-1]
    CCDS52398.1. [Q8BFR5-2]
    RefSeqiNP_001157185.1. NM_001163713.1. [Q8BFR5-2]
    NP_766333.1. NM_172745.3. [Q8BFR5-1]
    UniGeneiMm.197829.

    Genome annotation databases

    EnsembliENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838. [Q8BFR5-1]
    ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838. [Q8BFR5-2]
    GeneIDi233870.
    KEGGimmu:233870.
    UCSCiuc009jro.2. mouse. [Q8BFR5-1]
    uc009jrq.2. mouse. [Q8BFR5-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK075857 mRNA. Translation: BAC36008.1 .
    AK084724 mRNA. Translation: BAC39263.1 .
    AK153135 mRNA. Translation: BAE31747.1 .
    AK152858 mRNA. Translation: BAE31551.1 .
    BC060959 mRNA. Translation: AAH60959.1 .
    BC100596 mRNA. Translation: AAI00597.1 .
    CCDSi CCDS21830.1. [Q8BFR5-1 ]
    CCDS52398.1. [Q8BFR5-2 ]
    RefSeqi NP_001157185.1. NM_001163713.1. [Q8BFR5-2 ]
    NP_766333.1. NM_172745.3. [Q8BFR5-1 ]
    UniGenei Mm.197829.

    3D structure databases

    ProteinModelPortali Q8BFR5.
    SMRi Q8BFR5. Positions 55-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231459. 4 interactions.
    IntActi Q8BFR5. 8 interactions.
    MINTi MINT-1860932.

    PTM databases

    PhosphoSitei Q8BFR5.

    2D gel databases

    REPRODUCTION-2DPAGE Q6P919.
    Q8BFR5.

    Proteomic databases

    MaxQBi Q8BFR5.
    PaxDbi Q8BFR5.
    PRIDEi Q8BFR5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000098048 ; ENSMUSP00000095656 ; ENSMUSG00000073838 . [Q8BFR5-1 ]
    ENSMUST00000106392 ; ENSMUSP00000102000 ; ENSMUSG00000073838 . [Q8BFR5-2 ]
    GeneIDi 233870.
    KEGGi mmu:233870.
    UCSCi uc009jro.2. mouse. [Q8BFR5-1 ]
    uc009jrq.2. mouse. [Q8BFR5-2 ]

    Organism-specific databases

    CTDi 7284.
    MGIi MGI:1923686. Tufm.

    Phylogenomic databases

    eggNOGi COG0050.
    GeneTreei ENSGT00550000074682.
    HOGENOMi HOG000229290.
    HOVERGENi HBG001535.
    InParanoidi Q8BFR5.
    KOi K02358.
    OMAi GTEMCMP.
    OrthoDBi EOG73BVCN.
    PhylomeDBi Q8BFR5.
    TreeFami TF300432.

    Miscellaneous databases

    NextBioi 381887.
    PROi Q8BFR5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BFR5.
    CleanExi MM_TUFM.
    Genevestigatori Q8BFR5.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Heart and Tongue.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Kidney.
    3. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 91-102; 105-120; 210-227; 239-271; 316-327 AND 352-361, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.

    Entry informationi

    Entry nameiEFTU_MOUSE
    AccessioniPrimary (citable) accession number: Q8BFR5
    Secondary accession number(s): Q497E7, Q6P919
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3