SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8BFR5

- EFTU_MOUSE

UniProt

Q8BFR5 - EFTU_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Elongation factor Tu, mitochondrial
Gene
Tufm
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718GTP By similarity
Nucleotide bindingi126 – 1305GTP By similarity
Nucleotide bindingi181 – 1844GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. translation elongation factor activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. translational elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Gene namesi
Name:Tufm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1923686. Tufm.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: MGI
  2. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343Mitochondrion By similarity
Add
BLAST
Chaini44 – 452409Elongation factor Tu, mitochondrial
PRO_0000007463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791N6-acetyllysine By similarity
Modified residuei88 – 881N6-acetyllysine By similarity
Modified residuei256 – 2561N6-acetyllysine By similarity
Modified residuei418 – 4181N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BFR5.
PaxDbiQ8BFR5.
PRIDEiQ8BFR5.

2D gel databases

REPRODUCTION-2DPAGEQ6P919.
Q8BFR5.

PTM databases

PhosphoSiteiQ8BFR5.

Expressioni

Gene expression databases

BgeeiQ8BFR5.
CleanExiMM_TUFM.
GenevestigatoriQ8BFR5.

Interactioni

Protein-protein interaction databases

BioGridi231459. 4 interactions.
IntActiQ8BFR5. 8 interactions.
MINTiMINT-1860932.

Structurei

3D structure databases

ProteinModelPortaliQ8BFR5.
SMRiQ8BFR5. Positions 55-451.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 251197tr-type G
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 718G1 By similarity
Regioni105 – 1095G2 By similarity
Regioni126 – 1294G3 By similarity
Regioni181 – 1844G4 By similarity
Regioni219 – 2213G5 By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0050.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000229290.
HOVERGENiHBG001535.
InParanoidiQ8BFR5.
KOiK02358.
OMAiGTEMCMP.
OrthoDBiEOG73BVCN.
PhylomeDBiQ8BFR5.
TreeFamiTF300432.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BFR5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAATLLRAT PRFSGLCASP TPFLQGRLRP LKAPASPFLC RGLAVEAKKT    50
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE 100
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV 150
VAANDGPMPQ TREHLLLAKQ IGVEHVVVYV NKADAVQDSE MVELVELEIR 200
ELLTEFGYKG EETPVIVGSA LCALEQRDPE LGVKSVQKLL DAVDTYIPVP 250
TRDLDKPFLL PVESVYSIPG RGTVVTGTLE RGILKKGDEC ELLGHNKNIR 300
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIQPHQ 350
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRVI LPPGKELAMP 400
GEDLKLSLIL RQPMILEKGQ RFTLRDGNKT IGTGLVTDVP AMTEEDKNIK 450
WS 452
Length:452
Mass (Da):49,508
Last modified:March 1, 2003 - v1
Checksum:iF8687A05FFCB337D
GO
Isoform 2 (identifier: Q8BFR5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-452: YILSKEEGGR...TEEDKNIKWS → RAPVLSGFPC...TCHAWRGLEA

Note: No experimental confirmation available.

Show »
Length:435
Mass (Da):47,222
Checksum:i6821088F0D8A5145
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 45296YILSK…NIKWS → RAPVLSGFPCLEAGLVAKPF HPYCFSPLGLYPQQGGRWPP QTLCISFHARHVLPDLGHGL SSHLASREGTCHAWRGLEA in isoform 2.
VSP_013942Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK075857 mRNA. Translation: BAC36008.1.
AK084724 mRNA. Translation: BAC39263.1.
AK153135 mRNA. Translation: BAE31747.1.
AK152858 mRNA. Translation: BAE31551.1.
BC060959 mRNA. Translation: AAH60959.1.
BC100596 mRNA. Translation: AAI00597.1.
CCDSiCCDS21830.1. [Q8BFR5-1]
CCDS52398.1. [Q8BFR5-2]
RefSeqiNP_001157185.1. NM_001163713.1. [Q8BFR5-2]
NP_766333.1. NM_172745.3. [Q8BFR5-1]
UniGeneiMm.197829.

Genome annotation databases

EnsembliENSMUST00000098048; ENSMUSP00000095656; ENSMUSG00000073838. [Q8BFR5-1]
ENSMUST00000106392; ENSMUSP00000102000; ENSMUSG00000073838. [Q8BFR5-2]
GeneIDi233870.
KEGGimmu:233870.
UCSCiuc009jro.2. mouse. [Q8BFR5-1]
uc009jrq.2. mouse. [Q8BFR5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK075857 mRNA. Translation: BAC36008.1 .
AK084724 mRNA. Translation: BAC39263.1 .
AK153135 mRNA. Translation: BAE31747.1 .
AK152858 mRNA. Translation: BAE31551.1 .
BC060959 mRNA. Translation: AAH60959.1 .
BC100596 mRNA. Translation: AAI00597.1 .
CCDSi CCDS21830.1. [Q8BFR5-1 ]
CCDS52398.1. [Q8BFR5-2 ]
RefSeqi NP_001157185.1. NM_001163713.1. [Q8BFR5-2 ]
NP_766333.1. NM_172745.3. [Q8BFR5-1 ]
UniGenei Mm.197829.

3D structure databases

ProteinModelPortali Q8BFR5.
SMRi Q8BFR5. Positions 55-451.
ModBasei Search...

Protein-protein interaction databases

BioGridi 231459. 4 interactions.
IntActi Q8BFR5. 8 interactions.
MINTi MINT-1860932.

PTM databases

PhosphoSitei Q8BFR5.

2D gel databases

REPRODUCTION-2DPAGE Q6P919.
Q8BFR5.

Proteomic databases

MaxQBi Q8BFR5.
PaxDbi Q8BFR5.
PRIDEi Q8BFR5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000098048 ; ENSMUSP00000095656 ; ENSMUSG00000073838 . [Q8BFR5-1 ]
ENSMUST00000106392 ; ENSMUSP00000102000 ; ENSMUSG00000073838 . [Q8BFR5-2 ]
GeneIDi 233870.
KEGGi mmu:233870.
UCSCi uc009jro.2. mouse. [Q8BFR5-1 ]
uc009jrq.2. mouse. [Q8BFR5-2 ]

Organism-specific databases

CTDi 7284.
MGIi MGI:1923686. Tufm.

Phylogenomic databases

eggNOGi COG0050.
GeneTreei ENSGT00550000074682.
HOGENOMi HOG000229290.
HOVERGENi HBG001535.
InParanoidi Q8BFR5.
KOi K02358.
OMAi GTEMCMP.
OrthoDBi EOG73BVCN.
PhylomeDBi Q8BFR5.
TreeFami TF300432.

Miscellaneous databases

NextBioi 381887.
PROi Q8BFR5.
SOURCEi Search...

Gene expression databases

Bgeei Q8BFR5.
CleanExi MM_TUFM.
Genevestigatori Q8BFR5.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Heart and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Kidney.
  3. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 91-102; 105-120; 210-227; 239-271; 316-327 AND 352-361, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiEFTU_MOUSE
AccessioniPrimary (citable) accession number: Q8BFR5
Secondary accession number(s): Q497E7, Q6P919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi