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Q8BFR4

- GNS_MOUSE

UniProt

Q8BFR4 - GNS_MOUSE

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Protein

N-acetylglucosamine-6-sulfatase

Gene

Gns

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471CalciumBy similarity
Metal bindingi48 – 481CalciumBy similarity
Metal bindingi83 – 831Calcium; via 3-oxoalanineBy similarity
Metal bindingi318 – 3181CalciumBy similarity
Metal bindingi319 – 3191CalciumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. N-acetylglucosamine-6-sulfatase activity Source: UniProtKB-EC
  3. sulfuric ester hydrolase activity Source: MGI

GO - Biological processi

  1. glycosaminoglycan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198960. Keratan sulfate degradation.
REACT_231722. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosamine-6-sulfatase (EC:3.1.6.14)
Alternative name(s):
Glucosamine-6-sulfatase
Short name:
G6S
Gene namesi
Name:Gns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1922862. Gns.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 544509N-acetylglucosamine-6-sulfatasePRO_0000273189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 8313-oxoalanine (Cys)By similarity
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi472 – 4721N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BFR4.
PaxDbiQ8BFR4.
PRIDEiQ8BFR4.

PTM databases

PhosphoSiteiQ8BFR4.

Expressioni

Gene expression databases

BgeeiQ8BFR4.
CleanExiMM_GNS.
ExpressionAtlasiQ8BFR4. baseline and differential.
GenevestigatoriQ8BFR4.

Interactioni

Protein-protein interaction databases

IntActiQ8BFR4. 1 interaction.
MINTiMINT-4110937.

Structurei

3D structure databases

ProteinModelPortaliQ8BFR4.
SMRiQ8BFR4. Positions 39-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3119.
GeneTreeiENSGT00400000022041.
HOGENOMiHOG000169239.
HOVERGENiHBG005840.
InParanoidiQ8BFR4.
KOiK01137.
OMAiAPQYQKA.
OrthoDBiEOG75QR3Q.
PhylomeDBiQ8BFR4.
TreeFamiTF313545.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012251. GlcNAc_6-SO4ase.
IPR015981. GlcNAc_6-SO4ase_euk.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view]
PANTHERiPTHR10342:SF212. PTHR10342:SF212. 1 hit.
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036666. G6S. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BFR4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLPSAAGPR PGRPRRLPAL LLLPLLGGCL GLVGAARRPN VLLLLTDDQD
60 70 80 90 100
AELGGMTPLK KTKALIGEKG MTFSSAYVPS ALCCPSRASI LTGKYPHNHH
110 120 130 140 150
VVNNTLEGNC SSKAWQKIQE PYTFPAILKS VCGYQTFFAG KYLNEYGAPD
160 170 180 190 200
AGGLEHIPLG WSYWYALEKN SKYYNYTLSI NGKARKHGEN YSVDYLTDVL
210 220 230 240 250
ANLSLDFLDY KSNSEPFFMM ISTPAPHSPW TAAPQYQKAF QNVIAPRNKN
260 270 280 290 300
FNIHGTNKHW LIRQAKTPMT NSSIRFLDDA FRRRWQTLLS VDDLVEKLVK
310 320 330 340 350
RLDSTGELDN TYIFYTSDNG YHTGQFSLPI DKRQLYEFDI KVPLLVRGPG
360 370 380 390 400
IKPNQTSKML VSNIDLGPTI LDLAGYDLNK TQMDGMSLLP ILKGDRNLTW
410 420 430 440 450
RSDVLVEYQG EGRNVTDPTC PSLSPGVSQC FPDCVCEDAY NNTYACVRTL
460 470 480 490 500
SSLWNLQYCE FDDQEVFVEV YNITADPDQI TNIAKSIDPE LLGKMNYRLM
510 520 530 540
MLQSCSGPTC RTPGVFDPGY RFDLRLMFNS HGSVRTRRFS KHPL
Length:544
Mass (Da):61,175
Last modified:March 1, 2003 - v1
Checksum:iF9E95AFA6CBEA842
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → V in BAC35632. (PubMed:16141072)Curated
Sequence conflicti138 – 1381F → L in BAE35186. (PubMed:16141072)Curated
Sequence conflicti314 – 3141F → L in BAC38966. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030773 mRNA. Translation: BAC27129.1.
AK049162 mRNA. Translation: BAC33578.1.
AK054046 mRNA. Translation: BAC35632.1.
AK083597 mRNA. Translation: BAC38966.1.
AK159562 mRNA. Translation: BAE35186.1.
AK169485 mRNA. Translation: BAE41197.1.
AK165180 mRNA. Translation: BAE38063.1.
AK170791 mRNA. Translation: BAE42031.1.
BC055328 mRNA. Translation: AAH55328.1.
CCDSiCCDS24210.1.
RefSeqiNP_083640.1. NM_029364.3.
UniGeneiMm.207683.

Genome annotation databases

EnsembliENSMUST00000040344; ENSMUSP00000043167; ENSMUSG00000034707.
GeneIDi75612.
KEGGimmu:75612.
UCSCiuc007hfo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030773 mRNA. Translation: BAC27129.1 .
AK049162 mRNA. Translation: BAC33578.1 .
AK054046 mRNA. Translation: BAC35632.1 .
AK083597 mRNA. Translation: BAC38966.1 .
AK159562 mRNA. Translation: BAE35186.1 .
AK169485 mRNA. Translation: BAE41197.1 .
AK165180 mRNA. Translation: BAE38063.1 .
AK170791 mRNA. Translation: BAE42031.1 .
BC055328 mRNA. Translation: AAH55328.1 .
CCDSi CCDS24210.1.
RefSeqi NP_083640.1. NM_029364.3.
UniGenei Mm.207683.

3D structure databases

ProteinModelPortali Q8BFR4.
SMRi Q8BFR4. Positions 39-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BFR4. 1 interaction.
MINTi MINT-4110937.

PTM databases

PhosphoSitei Q8BFR4.

Proteomic databases

MaxQBi Q8BFR4.
PaxDbi Q8BFR4.
PRIDEi Q8BFR4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040344 ; ENSMUSP00000043167 ; ENSMUSG00000034707 .
GeneIDi 75612.
KEGGi mmu:75612.
UCSCi uc007hfo.1. mouse.

Organism-specific databases

CTDi 2799.
MGIi MGI:1922862. Gns.

Phylogenomic databases

eggNOGi COG3119.
GeneTreei ENSGT00400000022041.
HOGENOMi HOG000169239.
HOVERGENi HBG005840.
InParanoidi Q8BFR4.
KOi K01137.
OMAi APQYQKA.
OrthoDBi EOG75QR3Q.
PhylomeDBi Q8BFR4.
TreeFami TF313545.

Enzyme and pathway databases

Reactomei REACT_198960. Keratan sulfate degradation.
REACT_231722. Lysosome Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSi Gns. mouse.
NextBioi 343508.
PROi Q8BFR4.
SOURCEi Search...

Gene expression databases

Bgeei Q8BFR4.
CleanExi MM_GNS.
ExpressionAtlasi Q8BFR4. baseline and differential.
Genevestigatori Q8BFR4.

Family and domain databases

Gene3Di 3.40.720.10. 3 hits.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012251. GlcNAc_6-SO4ase.
IPR015981. GlcNAc_6-SO4ase_euk.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view ]
PANTHERi PTHR10342:SF212. PTHR10342:SF212. 1 hit.
Pfami PF00884. Sulfatase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036666. G6S. 1 hit.
SUPFAMi SSF53649. SSF53649. 2 hits.
PROSITEi PS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Oviduct and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiGNS_MOUSE
AccessioniPrimary (citable) accession number: Q8BFR4
Secondary accession number(s): Q3TWT0, Q8BJJ7, Q8BK91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3