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Q8BFR4 (GNS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylglucosamine-6-sulfatase

EC=3.1.6.14
Alternative name(s):
Glucosamine-6-sulfatase
Short name=G6S
Gene names
Name:Gns
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Lysosome By similarity.

Post-translational modification

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.

Sequence similarities

Belongs to the sulfatase family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycosaminoglycan metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-acetylglucosamine-6-sulfatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sulfuric ester hydrolase activity

Inferred from sequence orthology PubMed 15962010. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 544509N-acetylglucosamine-6-sulfatase
PRO_0000273189

Sites

Metal binding471Calcium By similarity
Metal binding481Calcium By similarity
Metal binding831Calcium; via 3-oxoalanine By similarity
Metal binding3181Calcium By similarity
Metal binding3191Calcium By similarity

Amino acid modifications

Modified residue8313-oxoalanine (Cys) By similarity
Glycosylation1031N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3541N-linked (GlcNAc...) Potential
Glycosylation3791N-linked (GlcNAc...) Potential
Glycosylation3971N-linked (GlcNAc...) Potential
Glycosylation4141N-linked (GlcNAc...) Potential
Glycosylation4411N-linked (GlcNAc...) Potential
Glycosylation4721N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict651L → V in BAC35632. Ref.1
Sequence conflict1381F → L in BAE35186. Ref.1
Sequence conflict3141F → L in BAC38966. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BFR4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F9E95AFA6CBEA842

FASTA54461,175
        10         20         30         40         50         60 
MRLPSAAGPR PGRPRRLPAL LLLPLLGGCL GLVGAARRPN VLLLLTDDQD AELGGMTPLK 

        70         80         90        100        110        120 
KTKALIGEKG MTFSSAYVPS ALCCPSRASI LTGKYPHNHH VVNNTLEGNC SSKAWQKIQE 

       130        140        150        160        170        180 
PYTFPAILKS VCGYQTFFAG KYLNEYGAPD AGGLEHIPLG WSYWYALEKN SKYYNYTLSI 

       190        200        210        220        230        240 
NGKARKHGEN YSVDYLTDVL ANLSLDFLDY KSNSEPFFMM ISTPAPHSPW TAAPQYQKAF 

       250        260        270        280        290        300 
QNVIAPRNKN FNIHGTNKHW LIRQAKTPMT NSSIRFLDDA FRRRWQTLLS VDDLVEKLVK 

       310        320        330        340        350        360 
RLDSTGELDN TYIFYTSDNG YHTGQFSLPI DKRQLYEFDI KVPLLVRGPG IKPNQTSKML 

       370        380        390        400        410        420 
VSNIDLGPTI LDLAGYDLNK TQMDGMSLLP ILKGDRNLTW RSDVLVEYQG EGRNVTDPTC 

       430        440        450        460        470        480 
PSLSPGVSQC FPDCVCEDAY NNTYACVRTL SSLWNLQYCE FDDQEVFVEV YNITADPDQI 

       490        500        510        520        530        540 
TNIAKSIDPE LLGKMNYRLM MLQSCSGPTC RTPGVFDPGY RFDLRLMFNS HGSVRTRRFS 


KHPL 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Oviduct and Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK030773 mRNA. Translation: BAC27129.1.
AK049162 mRNA. Translation: BAC33578.1.
AK054046 mRNA. Translation: BAC35632.1.
AK083597 mRNA. Translation: BAC38966.1.
AK159562 mRNA. Translation: BAE35186.1.
AK169485 mRNA. Translation: BAE41197.1.
AK165180 mRNA. Translation: BAE38063.1.
AK170791 mRNA. Translation: BAE42031.1.
BC055328 mRNA. Translation: AAH55328.1.
CCDSCCDS24210.1.
RefSeqNP_083640.1. NM_029364.3.
UniGeneMm.207683.

3D structure databases

ProteinModelPortalQ8BFR4.
SMRQ8BFR4. Positions 39-418.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BFR4. 1 interaction.
MINTMINT-4110937.

PTM databases

PhosphoSiteQ8BFR4.

Proteomic databases

MaxQBQ8BFR4.
PaxDbQ8BFR4.
PRIDEQ8BFR4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040344; ENSMUSP00000043167; ENSMUSG00000034707.
GeneID75612.
KEGGmmu:75612.
UCSCuc007hfo.1. mouse.

Organism-specific databases

CTD2799.
MGIMGI:1922862. Gns.

Phylogenomic databases

eggNOGCOG3119.
GeneTreeENSGT00400000022041.
HOGENOMHOG000169239.
HOVERGENHBG005840.
InParanoidQ8BFR4.
KOK01137.
OMAAPQYQKA.
OrthoDBEOG75QR3Q.
PhylomeDBQ8BFR4.
TreeFamTF313545.

Gene expression databases

ArrayExpressQ8BFR4.
BgeeQ8BFR4.
CleanExMM_GNS.
GenevestigatorQ8BFR4.

Family and domain databases

Gene3D3.40.720.10. 3 hits.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012251. GlcNAc_6-SO4ase.
IPR015981. GlcNAc_6-SO4ase_euk.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view]
PANTHERPTHR10342:SF212. PTHR10342:SF212. 1 hit.
PfamPF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFPIRSF036666. G6S. 1 hit.
SUPFAMSSF53649. SSF53649. 2 hits.
PROSITEPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGNS. mouse.
NextBio343508.
PROQ8BFR4.
SOURCESearch...

Entry information

Entry nameGNS_MOUSE
AccessionPrimary (citable) accession number: Q8BFR4
Secondary accession number(s): Q3TWT0, Q8BJJ7, Q8BK91
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot