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Q8BFR4

- GNS_MOUSE

UniProt

Q8BFR4 - GNS_MOUSE

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Protein

N-acetylglucosamine-6-sulfatase

Gene
Gns
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Calcium By similarity
Metal bindingi48 – 481Calcium By similarity
Metal bindingi83 – 831Calcium; via 3-oxoalanine By similarity
Metal bindingi318 – 3181Calcium By similarity
Metal bindingi319 – 3191Calcium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. N-acetylglucosamine-6-sulfatase activity Source: UniProtKB-EC
  3. sulfuric ester hydrolase activity Source: MGI

GO - Biological processi

  1. glycosaminoglycan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198960. Keratan sulfate degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosamine-6-sulfatase (EC:3.1.6.14)
Alternative name(s):
Glucosamine-6-sulfatase
Short name:
G6S
Gene namesi
Name:Gns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1922862. Gns.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535 Reviewed predictionAdd
BLAST
Chaini36 – 544509N-acetylglucosamine-6-sulfatasePRO_0000273189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 8313-oxoalanine (Cys) By similarity
Glycosylationi103 – 1031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi109 – 1091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi175 – 1751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi190 – 1901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi202 – 2021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi271 – 2711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi354 – 3541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi379 – 3791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi397 – 3971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi414 – 4141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi441 – 4411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi472 – 4721N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BFR4.
PaxDbiQ8BFR4.
PRIDEiQ8BFR4.

PTM databases

PhosphoSiteiQ8BFR4.

Expressioni

Gene expression databases

ArrayExpressiQ8BFR4.
BgeeiQ8BFR4.
CleanExiMM_GNS.
GenevestigatoriQ8BFR4.

Interactioni

Protein-protein interaction databases

IntActiQ8BFR4. 1 interaction.
MINTiMINT-4110937.

Structurei

3D structure databases

ProteinModelPortaliQ8BFR4.
SMRiQ8BFR4. Positions 39-418.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3119.
GeneTreeiENSGT00400000022041.
HOGENOMiHOG000169239.
HOVERGENiHBG005840.
InParanoidiQ8BFR4.
KOiK01137.
OMAiAPQYQKA.
OrthoDBiEOG75QR3Q.
PhylomeDBiQ8BFR4.
TreeFamiTF313545.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012251. GlcNAc_6-SO4ase.
IPR015981. GlcNAc_6-SO4ase_euk.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view]
PANTHERiPTHR10342:SF212. PTHR10342:SF212. 1 hit.
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036666. G6S. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BFR4-1 [UniParc]FASTAAdd to Basket

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MRLPSAAGPR PGRPRRLPAL LLLPLLGGCL GLVGAARRPN VLLLLTDDQD    50
AELGGMTPLK KTKALIGEKG MTFSSAYVPS ALCCPSRASI LTGKYPHNHH 100
VVNNTLEGNC SSKAWQKIQE PYTFPAILKS VCGYQTFFAG KYLNEYGAPD 150
AGGLEHIPLG WSYWYALEKN SKYYNYTLSI NGKARKHGEN YSVDYLTDVL 200
ANLSLDFLDY KSNSEPFFMM ISTPAPHSPW TAAPQYQKAF QNVIAPRNKN 250
FNIHGTNKHW LIRQAKTPMT NSSIRFLDDA FRRRWQTLLS VDDLVEKLVK 300
RLDSTGELDN TYIFYTSDNG YHTGQFSLPI DKRQLYEFDI KVPLLVRGPG 350
IKPNQTSKML VSNIDLGPTI LDLAGYDLNK TQMDGMSLLP ILKGDRNLTW 400
RSDVLVEYQG EGRNVTDPTC PSLSPGVSQC FPDCVCEDAY NNTYACVRTL 450
SSLWNLQYCE FDDQEVFVEV YNITADPDQI TNIAKSIDPE LLGKMNYRLM 500
MLQSCSGPTC RTPGVFDPGY RFDLRLMFNS HGSVRTRRFS KHPL 544
Length:544
Mass (Da):61,175
Last modified:March 1, 2003 - v1
Checksum:iF9E95AFA6CBEA842
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → V in BAC35632. 1 Publication
Sequence conflicti138 – 1381F → L in BAE35186. 1 Publication
Sequence conflicti314 – 3141F → L in BAC38966. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030773 mRNA. Translation: BAC27129.1.
AK049162 mRNA. Translation: BAC33578.1.
AK054046 mRNA. Translation: BAC35632.1.
AK083597 mRNA. Translation: BAC38966.1.
AK159562 mRNA. Translation: BAE35186.1.
AK169485 mRNA. Translation: BAE41197.1.
AK165180 mRNA. Translation: BAE38063.1.
AK170791 mRNA. Translation: BAE42031.1.
BC055328 mRNA. Translation: AAH55328.1.
CCDSiCCDS24210.1.
RefSeqiNP_083640.1. NM_029364.3.
UniGeneiMm.207683.

Genome annotation databases

EnsembliENSMUST00000040344; ENSMUSP00000043167; ENSMUSG00000034707.
GeneIDi75612.
KEGGimmu:75612.
UCSCiuc007hfo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030773 mRNA. Translation: BAC27129.1 .
AK049162 mRNA. Translation: BAC33578.1 .
AK054046 mRNA. Translation: BAC35632.1 .
AK083597 mRNA. Translation: BAC38966.1 .
AK159562 mRNA. Translation: BAE35186.1 .
AK169485 mRNA. Translation: BAE41197.1 .
AK165180 mRNA. Translation: BAE38063.1 .
AK170791 mRNA. Translation: BAE42031.1 .
BC055328 mRNA. Translation: AAH55328.1 .
CCDSi CCDS24210.1.
RefSeqi NP_083640.1. NM_029364.3.
UniGenei Mm.207683.

3D structure databases

ProteinModelPortali Q8BFR4.
SMRi Q8BFR4. Positions 39-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BFR4. 1 interaction.
MINTi MINT-4110937.

PTM databases

PhosphoSitei Q8BFR4.

Proteomic databases

MaxQBi Q8BFR4.
PaxDbi Q8BFR4.
PRIDEi Q8BFR4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040344 ; ENSMUSP00000043167 ; ENSMUSG00000034707 .
GeneIDi 75612.
KEGGi mmu:75612.
UCSCi uc007hfo.1. mouse.

Organism-specific databases

CTDi 2799.
MGIi MGI:1922862. Gns.

Phylogenomic databases

eggNOGi COG3119.
GeneTreei ENSGT00400000022041.
HOGENOMi HOG000169239.
HOVERGENi HBG005840.
InParanoidi Q8BFR4.
KOi K01137.
OMAi APQYQKA.
OrthoDBi EOG75QR3Q.
PhylomeDBi Q8BFR4.
TreeFami TF313545.

Enzyme and pathway databases

Reactomei REACT_198960. Keratan sulfate degradation.

Miscellaneous databases

ChiTaRSi GNS. mouse.
NextBioi 343508.
PROi Q8BFR4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BFR4.
Bgeei Q8BFR4.
CleanExi MM_GNS.
Genevestigatori Q8BFR4.

Family and domain databases

Gene3Di 3.40.720.10. 3 hits.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012251. GlcNAc_6-SO4ase.
IPR015981. GlcNAc_6-SO4ase_euk.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view ]
PANTHERi PTHR10342:SF212. PTHR10342:SF212. 1 hit.
Pfami PF00884. Sulfatase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036666. G6S. 1 hit.
SUPFAMi SSF53649. SSF53649. 2 hits.
PROSITEi PS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Oviduct and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiGNS_MOUSE
AccessioniPrimary (citable) accession number: Q8BFR4
Secondary accession number(s): Q3TWT0, Q8BJJ7, Q8BK91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi