Q8BFR4 (GNS_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 83.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: N-acetylglucosamine-6-sulfatase EC=3.1.6.14 Alternative name(s): Glucosamine-6-sulfatase Short name=G6S | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 544 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Catalytic activity | Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate. |
| Cofactor | Binds 1 calcium ion per subunit By similarity. |
| Subcellular location | Lysosome By similarity. |
| Post-translational modification | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity. |
| Sequence similarities | Belongs to the sulfatase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Lysosome |
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | glycosaminoglycan metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular_component | lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | N-acetylglucosamine-6-sulfatase activity Inferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW sulfuric ester hydrolase activityInferred from sequence orthology PubMed 15962010. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 35 | 35 | Potential | ||||||
| Chain | 36 – 544 | 509 | N-acetylglucosamine-6-sulfatase | PRO_0000273189 | |||||
Sites | |||||||||
| Metal binding | 47 | 1 | Calcium By similarity | ||||||
| Metal binding | 48 | 1 | Calcium By similarity | ||||||
| Metal binding | 83 | 1 | Calcium; via 3-oxoalanine By similarity | ||||||
| Metal binding | 318 | 1 | Calcium By similarity | ||||||
| Metal binding | 319 | 1 | Calcium By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 83 | 1 | 3-oxoalanine (Cys) By similarity | ||||||
| Glycosylation | 103 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 109 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 175 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 190 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 202 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 271 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 354 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 379 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 397 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 414 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 441 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 472 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 65 | 1 | L → V in BAC35632. Ref.1 | ||||||
| Sequence conflict | 138 | 1 | F → L in BAE35186. Ref.1 | ||||||
| Sequence conflict | 314 | 1 | F → L in BAC38966. Ref.1 | ||||||
Sequences
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References
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.  Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Oviduct and Thymus. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK030773 mRNA. Translation: BAC27129.1. AK049162 mRNA. Translation: BAC33578.1. AK054046 mRNA. Translation: BAC35632.1. AK083597 mRNA. Translation: BAC38966.1. AK159562 mRNA. Translation: BAE35186.1. AK169485 mRNA. Translation: BAE41197.1. AK165180 mRNA. Translation: BAE38063.1. AK170791 mRNA. Translation: BAE42031.1. BC055328 mRNA. Translation: AAH55328.1. |
| IPI | IPI00221426. |
| RefSeq | NP_083640.1. NM_029364.3. |
| UniGene | Mm.207683. |
3D structure databases | |
| ProteinModelPortal | Q8BFR4. |
| SMR | Q8BFR4. Positions 39-418. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q8BFR4. |
Proteomic databases | |
| PaxDb | Q8BFR4. |
| PRIDE | Q8BFR4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000040344; ENSMUSP00000043167; ENSMUSG00000034707. |
| GeneID | 75612. |
| KEGG | mmu:75612. |
| UCSC | uc007hfo.1. mouse. |
Organism-specific databases | |
| CTD | 2799. |
| MGI | MGI:1922862. Gns. |
Phylogenomic databases | |
| eggNOG | COG3119. |
| GeneTree | ENSGT00400000022041. |
| HOGENOM | HOG000169239. |
| HOVERGEN | HBG005840. |
| InParanoid | Q8BFR4. |
| KO | K01137. |
| OMA | MCGYQTF. |
| OrthoDB | EOG4NGGMF. |
Gene expression databases | |
| ArrayExpress | Q8BFR4. |
| Bgee | Q8BFR4. |
| CleanEx | MM_GNS. |
| Genevestigator | Q8BFR4. |
Family and domain databases | |
| Gene3D | 3.40.720.10. 3 hits. |
| InterPro | IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR012251. GlcNAc_6-SO4ase. IPR015981. GlcNAc_6-SO4ase_euk. IPR000917. Sulfatase. IPR024607. Sulfatase_CS. [Graphical view] |
| PANTHER | PTHR10342:SF5. PTHR10342:SF5. 1 hit. |
| Pfam | PF00884. Sulfatase. 1 hit. [Graphical view] |
| PIRSF | PIRSF036666. G6S. 1 hit. |
| SUPFAM | SSF53649. Alkaline_phosphatase_core. 1 hit. |
| PROSITE | PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| ChiTaRS | GNS. mouse. |
| NextBio | 343508. |
| SOURCE | Search... |
Entry information
| Entry name | GNS_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8BFR4 Secondary accession number(s): Q3TWT0, Q8BJJ7, Q8BK91 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |