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Q8BB26

- HEMA_CVP67

UniProt

Q8BB26 - HEMA_CVP67

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Protein
Hemagglutinin-esterase
Gene
HE
Organism
Porcine hemagglutinating encephalomyelitis virus (strain 67N) (HEV-67N)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Nucleophile By similarity
Active sitei228 – 2281Charge relay system By similarity
Active sitei329 – 3291Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPorcine hemagglutinating encephalomyelitis virus (strain 67N) (HEV-67N)
Taxonomic identifieri230237 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007546: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surface Reviewed prediction
Add
BLAST
Transmembranei393 – 41321Helical; Reviewed prediction
Add
BLAST
Topological domaini414 – 42411Intravirion Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 424406Hemagglutinin-esterase
PRO_0000037149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 65 By similarity
Glycosylationi54 – 541N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi89 – 891N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi113 ↔ 162 By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi197 ↔ 276 By similarity
Disulfide bondi205 ↔ 249 By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi307 ↔ 312 By similarity
Glycosylationi316 – 3161N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi347 ↔ 371 By similarity
Glycosylationi358 – 3581N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ8BB26.
SMRiQ8BB26. Positions 19-375.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first part By similarity
Add
BLAST
Regioni128 – 266139Receptor binding By similarity
Add
BLAST
Regioni267 – 379113Esterase domain second part By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BB26-1 [UniParc]FASTAAdd to Basket

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MFLLPRFCLV CSIIGTFGFE NPPTNVVSHF NDDWFLFGDS RSDCNHVVNT    50
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY 100
EGVNFTPYHA FKCTSAGNND IWMQNKGLFY TQVYKKMAVY RSLTLVNVPY 150
VYNGSAQPTA FCKSGSLILN NPAYIAREAN VGDYYYKSEA DFSLSGCDEY 200
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH 250
YLVLPSGNYL AISNELLLTV PTKAICLNKR KVFTPVQVVD SRWNNARQSD 300
NMTAVACQLP YCYFRNSTSN YVGIHDVNHG DAGFTSILSG LLYDSPCFSQ 350
QGVFRYDNVS TVWPLFPFGN CPTAASIISS DLPICVYDPL PIILLGILLG 400
VAVIVIVVLL LYFMVDNGIR QHYA 424
Length:424
Mass (Da):47,668
Last modified:March 1, 2003 - v1
Checksum:i891CA286ED1CC040
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY078417 Genomic RNA. Translation: AAL80030.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY078417 Genomic RNA. Translation: AAL80030.1 .

3D structure databases

ProteinModelPortali Q8BB26.
SMRi Q8BB26. Positions 19-375.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of the 3'-terminal end (8.1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus: comparison with other haemagglutinating coronaviruses."
    Sasseville A.M.-J., Boutin M., Gelinas A.-M., Dea S.
    J. Gen. Virol. 83:2411-2416(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVP67
AccessioniPrimary (citable) accession number: Q8BB26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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