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Q8BB04

- VP3_ROTRF

UniProt

Q8BB04 - VP3_ROTRF

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri10933 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000007179: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3PRO_0000368073Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 (Potential). Interacts with VP2 (By similarity).By similarityCurated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BB04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLALRHSV AQVYADTQIY THDETKDDYE NAFLISNLTT HNILYLNYSV
60 70 80 90 100
KTLQILNKSG IAAIEIQKND ELFTLIRCNF TYDYIDDIVY LHDYSYYTNN
110 120 130 140 150
EIRTDQHWVT KTNIEDYLLP GWKLMYVGYN GNDTRGHYNF SFKCQNAATD
160 170 180 190 200
DDAIIEYIYS NELDFQNFIL KKIKERMTTS LPIARLSNRV FRDKLFKTLV
210 220 230 240 250
SDHSRVVNVG PRNESMFTFL DHPSIKQFSN GPYLVKDTIK LKQERWLGKR
260 270 280 290 300
LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IIYMVGSAPS YWIHDVKQYS
310 320 330 340 350
DLKFETWDPL DTPYSDLHHK ELFYASDVTK LKDNSILYVD IRTDRENADW
360 370 380 390 400
KTWRKIVEEQ TANNLNIAYK YLSTGKAKVC CVKMTAMDLE LPISAKLLHH
410 420 430 440 450
PTTEIRSEFY LIMDIWDSKN TKRFIPKGVL YSYINNTITE NVFIQQPFKL
460 470 480 490 500
RTLRNEYVVA LYALSNDFNN REDVVKLVNN QKNALITVRI NNTFKDEPKV
510 520 530 540 550
GFKDIYDWTF LPTDFETNES IITSYDGCLG MFGLSISLAS KPTGNNHLFI
560 570 580 590 600
LSGTNKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL
610 620 630 640 650
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTNKA SIEGGRYYEH
660 670 680 690 700
APIELIYACR SAREFAKLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF
710 720 730 740 750
IGIKFKNIPY EYDVKVPHLT FGVLNISDSM VPDVVAILKK FKNELFRMDV
760 770 780 790 800
TTSYTYMLSD EIYVANVSGV LSTYFKLYNA FYKEQITFGQ SRMFIPHITL
810 820 830
SFSNKRVVRI GSTRLNIDFI YLRKIKGDTV FDMTE
Length:835
Mass (Da):98,021
Last modified:March 1, 2003 - v1
Checksum:i76D70E3AAB71101E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY116592 mRNA. Translation: AAM80568.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY116592 mRNA. Translation: AAM80568.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Bovine rotavirus RF gene 3 encoding VP3."
    Charpilienne A., Poncet D., Cohen J.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTRF
AccessioniPrimary (citable) accession number: Q8BB04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3