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Q8BB04

- VP3_ROTRF

UniProt

Q8BB04 - VP3_ROTRF

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri10933 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000007179: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3PRO_0000368073Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 By similarity.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BB04-1 [UniParc]FASTAAdd to Basket

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MKVLALRHSV AQVYADTQIY THDETKDDYE NAFLISNLTT HNILYLNYSV    50
KTLQILNKSG IAAIEIQKND ELFTLIRCNF TYDYIDDIVY LHDYSYYTNN 100
EIRTDQHWVT KTNIEDYLLP GWKLMYVGYN GNDTRGHYNF SFKCQNAATD 150
DDAIIEYIYS NELDFQNFIL KKIKERMTTS LPIARLSNRV FRDKLFKTLV 200
SDHSRVVNVG PRNESMFTFL DHPSIKQFSN GPYLVKDTIK LKQERWLGKR 250
LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IIYMVGSAPS YWIHDVKQYS 300
DLKFETWDPL DTPYSDLHHK ELFYASDVTK LKDNSILYVD IRTDRENADW 350
KTWRKIVEEQ TANNLNIAYK YLSTGKAKVC CVKMTAMDLE LPISAKLLHH 400
PTTEIRSEFY LIMDIWDSKN TKRFIPKGVL YSYINNTITE NVFIQQPFKL 450
RTLRNEYVVA LYALSNDFNN REDVVKLVNN QKNALITVRI NNTFKDEPKV 500
GFKDIYDWTF LPTDFETNES IITSYDGCLG MFGLSISLAS KPTGNNHLFI 550
LSGTNKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 600
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTNKA SIEGGRYYEH 650
APIELIYACR SAREFAKLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF 700
IGIKFKNIPY EYDVKVPHLT FGVLNISDSM VPDVVAILKK FKNELFRMDV 750
TTSYTYMLSD EIYVANVSGV LSTYFKLYNA FYKEQITFGQ SRMFIPHITL 800
SFSNKRVVRI GSTRLNIDFI YLRKIKGDTV FDMTE 835
Length:835
Mass (Da):98,021
Last modified:March 1, 2003 - v1
Checksum:i76D70E3AAB71101E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY116592 mRNA. Translation: AAM80568.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY116592 mRNA. Translation: AAM80568.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Bovine rotavirus RF gene 3 encoding VP3."
    Charpilienne A., Poncet D., Cohen J.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP3_ROTRF
AccessioniPrimary (citable) accession number: Q8BB04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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