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Q8B912 (RPOA_PRRSB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 16 chains:

  1. Nsp1
    EC=3.4.22.-
  2. Nsp1-alpha papain-like cysteine proteinase
    EC=3.4.22.-
    Alternative name(s):
    PCP1-alpha
  3. Nsp1-beta papain-like cysteine proteinase
    EC=3.4.22.-
    Alternative name(s):
    PCP1-beta
  4. Nsp2 cysteine proteinase
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    CP2
    Short name=CP
  5. Non-structural protein 3
    Short name=Nsp3
  6. 3C-like serine proteinase
    Short name=3CLSP
    EC=3.4.21.-
    Alternative name(s):
    Nsp4
  7. Non-structural protein 5-6-7
    Short name=Nsp5-6-7
  8. Non-structural protein 5
    Short name=Nsp5
  9. Non-structural protein 6
    Short name=Nsp6
  10. Non-structural protein 7-alpha
    Short name=Nsp7-alpha
  11. Non-structural protein 7-beta
    Short name=Nsp7-beta
  12. Non-structural protein 8
    Short name=Nsp8
  13. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    Nsp9
  14. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    Nsp10
  15. Non-structural protein 11
    Short name=Nsp11
  16. Non-structural protein 12
    Short name=Nsp12
Gene names
Name:rep
ORF Names:1a-1b
OrganismPorcine reproductive and respiratory syndrome virus (strain HB-1) (PRRSV)
Taxonomic identifier300563 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesArteriviridaeArterivirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length3961 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

Nsp1 is essential for viral subgenomic mRNA synthesis By similarity.

Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways By similarity.

Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation By similarity.

The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein By similarity.

The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nsp1: Host nucleus By similarity. Host cytoplasm By similarity.

Nsp1-alpha papain-like cysteine proteinase: Host nucleus By similarity. Host cytoplasm By similarity.

Nsp1-beta papain-like cysteine proteinase: Host cytoplasm By similarity.

Nsp2 cysteine proteinase: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 5-6-7: Host membrane; Multi-pass membrane protein Potential.

3C-like serine proteinase: Host cytoplasm Potential.

RNA-directed RNA polymerase: Host cytoplasmhost perinuclear region Potential.

Helicase: Host cytoplasmhost perinuclear region Potential.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

The OTU-like region is responsible for the deubiquitinating and deISGylation activities of Nsp2 By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. Nsp1 is autocleaved into two subunits, Nsp1-alpha and Nsp1-beta. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4 By similarity.

Sequence similarities

Belongs to the arteriviridae polyprotein family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 AV MBD (arterivirus metal-binding) domain.

Contains 1 peptidase C31 domain.

Contains 1 peptidase C32 domain.

Contains 1 peptidase C33 domain.

Contains 1 peptidase S32 domain.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAN73221.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processHost-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Inhibition of host NF-kappa-B by virus
Inhibition of host STAT1 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Host nucleus
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainTransmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionHelicase
Hydrolase
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Serine protease
Thiol protease
Transferase
   Technical termMultifunctional enzyme
Gene Ontology (GO)
   Biological_processmodulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host NF-kappaB transcription factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host STAT1 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: Q8B912-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: Q8B912-2)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform differs from the canonical sequence as follows:
     2504-3961: Missing.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 39613961Replicase polyprotein 1ab
PRO_0000036670
Chain1 – 382382Nsp1 By similarity
PRO_0000410827
Chain1 – 180180Nsp1-alpha papain-like cysteine proteinase Potential
PRO_0000036672
Chain181 – 383203Nsp1-beta papain-like cysteine proteinase By similarity
PRO_0000036673
Chain384 – 15791196Nsp2 cysteine proteinase By similarity
PRO_0000036674
Chain1580 – 1809230Non-structural protein 3 By similarity
PRO_0000036675
Chain1810 – 20132043C-like serine proteinase By similarity
PRO_0000036676
Chain2014 – 2458445Non-structural protein 5-6-7 By similarity
PRO_0000036677
Chain2014 – 2183170Non-structural protein 5 By similarity
PRO_0000423122
Chain2184 – 219916Non-structural protein 6 By similarity
PRO_0000423123
Chain2200 – 2348149Non-structural protein 7-alpha By similarity
PRO_0000423124
Chain2349 – 2458110Non-structural protein 7-beta By similarity
PRO_0000423125
Chain2459 – 3144686RNA-directed RNA polymerase By similarity
PRO_0000036678
Chain2459 – 250345Non-structural protein 8 By similarity
PRO_0000036679
Chain3145 – 3585441Helicase By similarity
PRO_0000036680
Chain3586 – 3808223Non-structural protein 11 By similarity
PRO_0000036681
Chain3809 – 3961153Non-structural protein 12 By similarity
PRO_0000036682

Regions

Transmembrane1266 – 128621Helical; Potential
Transmembrane1296 – 131621Helical; Potential
Transmembrane1368 – 138821Helical; Potential
Transmembrane1583 – 160321Helical; Potential
Transmembrane1648 – 166821Helical; Potential
Transmembrane1685 – 170521Helical; Potential
Transmembrane1719 – 173921Helical; Potential
Transmembrane2036 – 205621Helical; Potential
Transmembrane2060 – 208021Helical; Potential
Transmembrane2092 – 211221Helical; Potential
Transmembrane2137 – 215721Helical; Potential
Transmembrane2162 – 218221Helical; Potential
Domain69 – 180112Peptidase C31
Domain263 – 383121Peptidase C32
Domain428 – 535108Peptidase C33
Domain1810 – 2013204Peptidase S32
Domain2890 – 3024135RdRp catalytic
Domain3145 – 320864AV MBD
Domain3265 – 3417153(+)RNA virus helicase ATP-binding
Domain3418 – 3546129(+)RNA virus helicase C-terminal
Zinc finger8 – 2821C4-type; atypical
Nucleotide binding3293 – 33008ATP By similarity
Region69 – 182114PCP1-alpha
Region263 – 382120PCP1-beta
Region426 – 51388OTU-like
Region1266 – 1388123HD1
Region1583 – 1745163HD2
Region2036 – 2157122HD3
Compositional bias808 – 924117Pro-rich
Compositional bias2329 – 234416Pro-rich

Sites

Active site761For Nsp1-alpha papain-like cysteine proteinase activity By similarity
Active site1461For Nsp1-alpha papain-like cysteine proteinase activity By similarity
Active site2701For Nsp1-beta papain-like cysteine proteinase activity By similarity
Active site3391For Nsp1-beta papain-like cysteine proteinase activity By similarity
Active site4371For Nsp2 cysteine proteinase activity By similarity
Active site5061For Nsp2 cysteine proteinase activity By similarity
Active site18481Charge relay system; for 3C-like serine proteinase activity By similarity
Active site18731Charge relay system; for 3C-like serine proteinase activity By similarity
Active site19271Charge relay system; for 3C-like serine proteinase activity By similarity
Site180 – 1812Cleavage; by autolysis By similarity
Site383 – 3842Cleavage; by autolysis By similarity
Site1579 – 15802Cleavage; by CP2 Potential
Site1809 – 18102Cleavage; by 3CLSP By similarity
Site2013 – 20142Cleavage; by 3CLSP By similarity
Site2183 – 21842Cleavage; by 3CLSP By similarity
Site2199 – 22002Cleavage; by 3CLSP By similarity
Site2348 – 23492Cleavage; by 3CLSP By similarity
Site2458 – 24592Cleavage; by 3CLSP By similarity
Site3144 – 31452Cleavage; by 3CLSP By similarity
Site3585 – 35862Cleavage; by 3CLSP By similarity
Site3808 – 38092Cleavage; by 3CLSP By similarity

Natural variations

Alternative sequence2504 – 39611458Missing in isoform Replicase polyprotein 1a.
VSP_032891

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified August 10, 2010. Version 3.
Checksum: 45D39828CC48EA77

FASTA3,961433,077
        10         20         30         40         50         60 
MSGILDRCTC TPNARVFVAE GQVYCTRCLS ARSLLPLNLQ VPELGVLGLF YRPEEPLRWT 

        70         80         90        100        110        120 
LPRAFPTVEC SPTGACWLSA IFPIARMTSG NLNFQQRMVR VAGEIYRAGQ LTPTVLKTIQ 

       130        140        150        160        170        180 
VYERGCRWYP IVGPVPGVGV YANSLHVSDK PFPGATHVLT NLPLPQRPKP EDFCPFECAM 

       190        200        210        220        230        240 
ADVYDIGRGA VMYVAGGKVS WAPRGGDEVK FEPVPKELKL VANRLHTSFP PHHVVDMSKF 

       250        260        270        280        290        300 
TFMTPGSGVS MRVEYQYGCL PADTVPEGNC WWRLFDLLPP EVQNKEIRHA NQFGYQTKHG 

       310        320        330        340        350        360 
VPGKYLQRRL QVNGLRAVTD THGPIVIQYF SVKESWIRHL KPVEEPSLPG FEDLLRIRVE 

       370        380        390        400        410        420 
PNTSPLAGKN EKIFRFGSHK WYGAGKRARK ARSGATTMVA HRASSAHETR QATKHEGAGA 

       430        440        450        460        470        480 
NKAEHLKLYS PPAEGNCGWH CISAIVNRMV NSNFETTLPE RVRPPDDWAT DEDLVNTIQI 

       490        500        510        520        530        540 
LRLPAALDRN GACGGAKYVL KLEGEHWTVS VNPGMSPSLL PLECVQGCCE HKGGLGSPDA 

       550        560        570        580        590        600 
VEVSGFDPAC LDRLLQVMHL PSSTIPAALA ELSDDSNRPV SPAAATWTVS QSYARHRGGN 

       610        620        630        640        650        660 
HHDQVCLGKI ISLCQVIEDC CCHQNKTNRA TPEEVAAKID QYLRGATSLE ECLAKLERVS 

       670        680        690        700        710        720 
PPGAADTSFD WNVVLPGVEA AHQTTEQLHV NPCRTLVPPV TQEPLGKDSV PLTAFSLSNC 

       730        740        750        760        770        780 
YYPAQGNEVR HRERLNSVLS KLEEVVLEEY GLMSTGLGPR PVLPSGLDEL KDQMEEDLLK 

       790        800        810        820        830        840 
LANTQATSEM MAWAAEQVDL KAWVKSYPRW TPPPPPPRVQ PRKTKSVKSL PEDKPVPAPR 

       850        860        870        880        890        900 
RKVRSGCGSP VLMGDNVPNG SEDLTVGGPL NFPTPSEPMT PMSEPVLTPA LQRVPKLMTP 

       910        920        930        940        950        960 
LDGSAPVPAP RRTVSRPMTP LSEPIFLSAP RHKFQQVEEA NPATTTLTHQ NEPLDLSASS 

       970        980        990       1000       1010       1020 
QTEYEASPLA SSQNMSILEA GGQEAEEVLS EISDILNDTS PAPVSSSSSL SSVKITRPKY 

      1030       1040       1050       1060       1070       1080 
SAQAIIDSGG PCSGHLQKEK EACLSIMREA CDASKLSDPA TQEWLSRMWD RVDMLTWRNT 

      1090       1100       1110       1120       1130       1140 
SAYQAFRTLN GRFEFLPKMI LETPPPHPCG FVMLPHTPAP SVSAESDLTI GSVATEDVPR 

      1150       1160       1170       1180       1190       1200 
ILGKIGDTGE LLNQGPSAPF KGGPVCDQPA KNSRMSPRES DESIIAPPAD TGGAGSFTDL 

      1210       1220       1230       1240       1250       1260 
PSSDSVDANG GGPLRTVKTK AGRLLDQLSC QVFSLVSHLP VFFSHLFKSD SGYSPGDWGF 

      1270       1280       1290       1300       1310       1320 
AAFTLFCLFL CYSYPFFGFA PLLGVFSGSS RRVRMGVFGC WLAFAVGLFK PVSDPVGTAC 

      1330       1340       1350       1360       1370       1380 
EFDSPECRNV LHSFELLKPW DPVRSLVVGP VGLGLAILGR LLGGARYVWH FLLRFGIVAD 

      1390       1400       1410       1420       1430       1440 
CILAGAYVLS QGRCKKCWGS CVRTAPNEIA FNVFPFTRAT RSSLIDLCDR FCAPKGMDPI 

      1450       1460       1470       1480       1490       1500 
FLATVWRGCW TGRSPIEQPS EKPIAFAQLD EKRITARTVV AQPYDPNQAV KCLRVLQAGG 

      1510       1520       1530       1540       1550       1560 
AMVAEAVPKV VKVSAIPFRA PFFPAGVKVD PECRIVVDPD TFTTALRSGY STTNLVLGMG 

      1570       1580       1590       1600       1610       1620 
DFAQLNGLKI RQISKPSGGG SHLVAALHVA CSMALHMLAG VYVTAVGSCG TGTNDPWCTN 

      1630       1640       1650       1660       1670       1680 
PFAAPGYGPG SLCTSRLCIS QHGLTLPLTA LVAGFGLQEI ALVVLIFVSM GGMAHRLSCK 

      1690       1700       1710       1720       1730       1740 
ADMLCILLAI ASYVWVPLTW LLCVFPCWLR WFSLHPLTIL WLVFFLISVN IPSGILAVVL 

      1750       1760       1770       1780       1790       1800 
LVSLWLLGRY TNIAGLVTPY DIHHYTSGPR GVAALATAPD GTYLAAVRRA ALTGRTMLFT 

      1810       1820       1830       1840       1850       1860 
PSQLGSLLEG AFRTQKPSLN TVNVVGSSMG SGGVFTIDGK IKCVTAAHVL TGNSARVSGV 

      1870       1880       1890       1900       1910       1920 
GFNQMLDFDV KGDFAIADCP NWQGAAPKAQ FCEDGWTGRA YWLTSSGVEP GVIGNGFAFC 

      1930       1940       1950       1960       1970       1980 
FTACGDSGSP VITEAGELVG VHTGSNKQGG GIVTRPSGQF CNVTPIKLSE LSEFFAGPKV 

      1990       2000       2010       2020       2030       2040 
PLGDVKIGSH IIKDTCEVPS DLCALLAAKP ELEGGLSTVQ LLCVFFLLWR MMGHAWTPLV 

      2050       2060       2070       2080       2090       2100 
AVGFFILNEI LPAVLVRSVF SFGMFVLSWL TPWSAQVLMI RLLTAALNRN RLSLGFYSLG 

      2110       2120       2130       2140       2150       2160 
AVTSFVADLA VTQGHPLQVV MNLSTYAFLP RMMVVTSPVP VIACGVVHLL AIILYLFKYR 

      2170       2180       2190       2200       2210       2220 
CLHYVLVGDG VFSSAFFLRY FAEGKLREGV SQSCGMSHES LTGALAMRLT DEDLDFLTKW 

      2230       2240       2250       2260       2270       2280 
TDFKCFVSAS NMRNAAGQFI EAAYAKALRI ELAQLVQVDK VRGTLAKLEA FADTVAPQLS 

      2290       2300       2310       2320       2330       2340 
PGDIVVALGH TPVGSIFDLK VGSTKHTLQA IETRVLAGSK MTVARVVDPT PAPPPVPVPI 

      2350       2360       2370       2380       2390       2400 
PLPPKVLENG PNAWGDEDRL NKKKRRRMEA VGIFVMDGKK YQKFWDKNSG DVFYEEVHNS 

      2410       2420       2430       2440       2450       2460 
TDEWECLRAG DPADFDPETG VQCGHITIED RVYNVFTSPS GRKFLVPANP ENRRAQWEAA 

      2470       2480       2490       2500       2510       2520 
KLSVEQALGM MNVDGELTAK ELEKLKGIID KLQGLTKEQC LNCLLAASGL TRCGRGGLVV 

      2530       2540       2550       2560       2570       2580 
TETAVKIVKF HNRTFTLGPV NLKVASEVEL KDAVEHNQHP VARPVDGGVV LLRSAVPSLI 

      2590       2600       2610       2620       2630       2640 
DVLISGADAS PKLLARHGPG NTGIDGTLWD FEAEATKEEV ALSAQIIQAC DIRRGDAPEI 

      2650       2660       2670       2680       2690       2700 
GLPYKLYPVR GNPERVKGVL QNTRFGDIPY KTPSDTGSPV HAAACLTPNA TPVTDGRSVL 

      2710       2720       2730       2740       2750       2760 
ATTMPSGFEL YVPTIPASVL DYLDSRPDCP KQLTEHGCED AALRDLSKYD LVTQGFVLPG 

      2770       2780       2790       2800       2810       2820 
VLRLVRKYLF AHVGKCPPVH RPSTYPAKNS MAGINGNRFP TKDIQSVPEI DVLCAQAVRE 

      2830       2840       2850       2860       2870       2880 
NWQTVTPCTL KKQYCGKKKT RTILGTNNFI ALAHRAALSG VTQGFMKKAF NSPIALGKNK 

      2890       2900       2910       2920       2930       2940 
FKELQTPVLG RCLEADLASC DRSTPAIVRW FAANLLYELA CAEEHLPSYV LNCCHDLLVT 

      2950       2960       2970       2980       2990       3000 
QSGAVTKRGG LSSGDPITSV SNTIYSLVIY AQHMVLSYFK SGHPHGLLFL QDQLKFEDML 

      3010       3020       3030       3040       3050       3060 
KVQPLIVYSD DLVLYAESPS MPNYHWWVEH LNLMLGFQTD PKKTAITDSP TFLGCRIING 

      3070       3080       3090       3100       3110       3120 
RQLVPNRDRI LAALAYHMKA SNVSEYYASA AAILMDSCAC LEYDPEWFEE LVVGIAQCAR 

      3130       3140       3150       3160       3170       3180 
KDGYSFPGPP FFLSMWEKLR SNHEGKKSRM CGYCMAPAPY ATACGLDVCV YHTHFHQHCP 

      3190       3200       3210       3220       3230       3240 
VIIWCGHPAG SGSCGECEPP LGKGTSPLDE VLEQVPYKPP RTVIMHVEQG LTPLDPGRYQ 

      3250       3260       3270       3280       3290       3300 
TRRGLVSVRR GIRGNEVDLP DGDYASTALL PTCKEINMVA VAPNVLRSRF IIGPPGAGKT 

      3310       3320       3330       3340       3350       3360 
HWLLQQVQDG DVIYTPTHQT MLDMIRALGT CRFNVPAGTT LQFPAPSRTG PWVRILAGGW 

      3370       3380       3390       3400       3410       3420 
CPGKNSFLDE AAYCNHLDVL RLLSKTTLTC LGDFKQLHPV GFDSHCYVFD IMPQTQLKTI 

      3430       3440       3450       3460       3470       3480 
WRFGQNICDA IQPDYRDKLV SMVNTTRVTY VEKPVRYGQV LTPYHRDRED GAITIDSSQG 

      3490       3500       3510       3520       3530       3540 
ATFDVVTLHL PTKDSLNRQR ALVAITRARH AIFVYDPHRQ LQSMFDLPAK GTPVNLAVHR 

      3550       3560       3570       3580       3590       3600 
DEQLIVLDRN NKEITVAQAL GNGDKFRATD KRVVDSLRAI CADLEGSSSP LPKVAHNLGF 

      3610       3620       3630       3640       3650       3660 
YFSPDLTQFA KLPAELAPHW PVVTTQNNER WPDRLVASLR PIHKYSRACI GAGYMVGPSV 

      3670       3680       3690       3700       3710       3720 
FLGTPGVVSY YLTKFVRGEA QVLPETVFST GRIEVDCREY LDDREREVAE SLPHAFIGDV 

      3730       3740       3750       3760       3770       3780 
KGTTVGGCHH VTSKYLPRFL PKESVAVVGV SSPGEAAKAF CTLTDVYLPD LEAYLHPETQ 

      3790       3800       3810       3820       3830       3840 
SKCWKVMLDF KEVRLMVWKG KTAYFQLEGR HFTWYQLASY TSYIRVPVNS TVYLDPCMGP 

      3850       3860       3870       3880       3890       3900 
ALCNRRVVGS THWGADLAVT PYDYGAKIIL SSAYHGEMPP GYKILACAEF SLDDPVRYKH 

      3910       3920       3930       3940       3950       3960 
TWGFESDTAY LYEFTGNGED WEDYNGAFRA RQKGKIYKAT ATSMKFHFPP GPVIEPTLGL 


N 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

Checksum: 335ED690575FF4BE
Show »

FASTA2,503271,842

References

[1]"Genomic characterization of two Chinese isolates of porcine respiratory and reproductive syndrome virus."
Gao Z.Q., Guo X., Yang H.C.
Arch. Virol. 149:1341-1351(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY150312 mRNA. Translation: AAN73220.1.
AY150312 mRNA. Translation: AAN73221.1. Different initiation.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR008743. Arterivirus_Nsp2_C33.
IPR023338. Arterivirus_NSP4_peptidase.
IPR027355. AV_MBD_dom.
IPR008741. AV_PCPalpha.
IPR025773. AV_PCPbeta.
IPR008760. EAV_peptidase_S32.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF05410. Peptidase_C31. 1 hit.
PF05411. Peptidase_C32. 1 hit.
PF05412. Peptidase_C33. 1 hit.
PF05579. Peptidase_S32. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS51538. AV_CP. 1 hit.
PS51652. AV_MBD. 1 hit.
PS51493. AV_NSP4_PRO. 1 hit.
PS51539. AV_PCP_ALPHA. 1 hit.
PS51540. AV_PCP_BETA. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOA_PRRSB
AccessionPrimary (citable) accession number: Q8B912
Secondary accession number(s): Q8B911
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: August 10, 2010
Last modified: April 16, 2014
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries