Q8B6J5 (L_RABVH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large structural protein
Short name=Protein L

Alternative name(s):
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Rabies virus (strain HEP-Flury) (RABV) [Complete proteome]

Taxonomic identifier

11296 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	2127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyltransferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyltransferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity. Ref.2
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviruses protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2127	2127	Large structural protein		PRO_0000294421
Regions
	Domain	611 – 799	189	RdRp catalytic
	Region	1562 – 2127	566	Interaction with P protein

Sequences

	Sequence		Length	Mass (Da)
	Q8B6J5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E9B31C8642375518	FASTA	2,127	242,955
	10 20 30 40 50 60 MLDPGEVYDD PIDPIESEAE PRGTPTVPNI LRNSDYNLNS PLIEDSAKLM LEWLKTGNRP 70 80 90 100 110 120 YRMTLTDNCS RSYKDLKDYF KKVDLGSLKV GGTAAQSMIS LWLYGAHSES NRSRRCITDL 130 140 150 160 170 180 AHFYSKSSPI EKLLNCTLGN RGLRIPPEGV LSCLERVDYD KAFGRYLANT YSSYLFFHVI 190 200 210 220 230 240 TLYMNALDWE EEKTILALWK DLTSVDTGKD LVKFKDQIWG LLIVTKDFVY SQSSNCLFDR 250 260 270 280 290 300 NYTLMLKDLF LSRFNSLMIL LSPPEPRYSD DLISQLCQLY IAGDQVLSLC GNSGYEVIKI 310 320 330 340 350 360 LEPYVVNSLV QRAEKFRPLI HSLGDFPMFI KDKVNQLEGT FGPSAKRFFR VLDQFDNIHD 370 380 390 400 410 420 LVFVYGCYRH WGHPYIDYRK GLLKLYDQVH IKKVIDKSYQ ECLASDLARR ILRWGFDKYS 430 440 450 460 470 480 KWYLDSRFLA RDHPLAPYIK TQTWPPKHIV DLVGDTWHKL PITQIFEIPE SMDPSEILDD 490 500 510 520 530 540 KSHSFTRTRL ASWLSENRGG PVPSEKVIIT ALSQPPVNPR EFLKSIDLGG LPDDDLIIGL 550 560 570 580 590 600 RPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 610 620 630 640 650 660 TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TEDVFSVLDQ VFGLKRVFSR THEFFQKSWI 670 680 690 700 710 720 YYSDRSDLIG LREDQIYCLD MSNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 730 740 750 760 770 780 RTKILAQGDN QVLCPTYMLS PGLSQEGLLY ELESISRNAL SIYRAIEEGA SKLGLIIKKE 790 800 810 820 830 840 ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 850 860 870 880 890 900 SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAE GESFLLAMSR IIYLDPSLGG 910 920 930 940 950 960 VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSHESWIHAL CQEAGNPDLG ERTLESFTRL 970 980 990 1000 1010 1020 LEDPTTLNIK GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSV 1030 1040 1050 1060 1070 1080 EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRKS LSRTLEESFY NSEIHGINRM 1090 1100 1110 1120 1130 1140 TQTPQRVGRV WPCSSERADL LREISWGRKV VGTTVPHPSE MLGLLPKSSI SCTCGATGGG 1150 1160 1170 1180 1190 1200 NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RAISLKESIN 1210 1220 1230 1240 1250 1260 WFINRNSNLA QTLIRNIMSL TGPDFSLEEA PVFKRTGSAL HRFKSARYSE GGYSSVCPNL 1270 1280 1290 1300 1310 1320 LSHISVSTDT MSDLTQDGKN YDFMFQPLML YAQTWTSELV QRDTRLRDST FHWHLRCNRC 1330 1340 1350 1360 1370 1380 VRPIEDITLE TSQIFEFPDV SKRISRMVSG AVPHFQKLPD IRLRPGDFES LSGREKSRHI 1390 1400 1410 1420 1430 1440 GSAQGLLYSI LVAIHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGILIGS SICFLTRMTN 1450 1460 1470 1480 1490 1500 INIKRPLELI SGVISYILLR LDNHPSLYIM LREPSLRGEI FSIPQKIPAA YPTTMKEGNR 1510 1520 1530 1540 1550 1560 SILCYLQHVL RYEREVITAS PENDWLWIFS DFRSAKMTYL TLITYQSHLL LQRVERNLSK 1570 1580 1590 1600 1610 1620 SMRATLQQMG SLMRQVLGGH GEDTLESDDD IQRLLKDSLR RTRWVDQEVR HAARTMSGDY 1630 1640 1650 1660 1670 1680 SPNKRVSRKA GCSEWVCSAQ QVAVSTSANP APVSELDIRA LSKRFQNPLI SGLRVVQWAT 1690 1700 1710 1720 1730 1740 GAHYKLKPIL DDLNVFPSLC LVVGDGSGGI SRAVLNMFPD SKLVFNSLLE VNDLMASGTR 1750 1760 1770 1780 1790 1800 PLPPSAIMSG GDDIISRVID FDSIWEKPSD LRNLATWRYF QSVQKQVNMS YDLIICDAEV 1810 1820 1830 1840 1850 1860 TDIASINRIT LLMSDFALSI DGPLYLVFKT YGTMLVNPDY KAIQHLSRAF PSVTGFITQV 1870 1880 1890 1900 1910 1920 TSSFSSELYL RFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR 1930 1940 1950 1960 1970 1980 GFPEEIISNP YNEMIITLID SDVESFLVHK MVDDLELQRG TLSKVAIIIS IMIVFSNRVF 1990 2000 2010 2020 2030 2040 NISKPLTDPL FYPPSDPKIL RHFNICCSTM MYLSTALGDV PSFARLHDLY NRPITYYFRK 2050 2060 2070 2080 2090 2100 QVIRGNIYLS WSWSDDTPVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG RIEDLSGEVE 2110 2120 RHLHGYNRWI TLEDIRSRSS LLDYSCL

References

[1]	"An improved method for recovering rabies virus from cloned cDNA." Inoue K., Shoji Y., Kurane I., Iijima T., Sakai T., Morimoto K. J. Virol. Methods 107:229-236(2003) [PubMed: 12505638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Mapping the interacting domains between the rabies virus polymerase and phosphoprotein." Chenik M., Schnell M., Conzelmann K.K., Blondel D. J. Virol. 72:1925-1930(1998) [PubMed: 9499045] [Abstract] Cited for: INTERACTION WITH PROTEIN P. Strain: CVS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB085828 Genomic RNA. Translation: BAC53869.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_RABVH

Accession

Primary (citable) accession number: Q8B6J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2003
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program