ID   L_VSIVN                 Reviewed;        2109 AA.
AC   Q8B0I0;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Vesicular stomatitis Indiana virus (strain 98COE North America)
OS   (VSIV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Dimarhabdovirus supergroup; Vesiculovirus.
OX   NCBI_TaxID=434488;
OH   NCBI_TaxID=7158; Aedes.
OH   NCBI_TaxID=7190; Simuliidae (black flies).
OH   NCBI_TaxID=7370; Musca domestica (House fly).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9793; Equus asinus (Donkey).
OH   NCBI_TaxID=9796; Equus caballus (Horse).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=58271; Culicoides.
OH   NCBI_TaxID=252607; Lutzomyia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=22222605; PubMed=12237430;
RA   Rodriguez L.L., Pauszek S.J., Bunch T.A., Schumann K.R.;
RT   "Full-length genome analysis of natural isolates of vesicular
RT   stomatitis virus (Indiana 1 serotype) from North, Central and South
RT   America.";
RL   J. Gen. Virol. 83:2475-2483(2002).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein to form the functional
CC       polymerase (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; AF473864; AAN16984.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   2109       Large structural protein.
FT                                /FTId=PRO_0000287264.
FT   DOMAIN      598    784       RdRp catalytic.
SQ   SEQUENCE   2109 AA;  240728 MW;  ADE79A01866DEEE0 CRC64;
     MEVHDFETDE FNDFNEDDYA TREFLNPDER MTYLNHADYN LNSPLISDDI DNLIRKFNSL
     PIPSMWDSKN WDGVLEMLTS CQANPIPTSR MHKWMGNWLM SDNHDASQGY SFLHEVDKEA
     EITFDVVETF IRGWGNKPIE YIKKEKWTDS FKILAYLCQK FLDLHKLTLI LNAVSEVELL
     NLARTFKGKV RRSSHGTNIC RLRVPSLGPT FISEGWAYFK KLDILMDRNF LLMVKDVIIG
     RMQTVLSMVG RIDNLFSEQD IFSLLNIYRI GDKIVERLGN FSYDLIKMVE PICNLKLMKL
     ARESRPLVPQ FPHFENHIKT SVDEGAKIDR GINFLHDQIM SVKTVDLTLV IYGSFRHWGH
     PFIDYYAGLE KLHSQVTMKK DIDVSYAKAL ASDLARIVLY QQFNDHKKWF VNGDLLPHDH
     PFKSHVKENT WPTAAQVQDF GDKWHELPLI KCFEIPDLLD PSIIYSDKSH SMNRSEVLKH
     VRMNPNTPIP SKKVLQTMLD TKATNWKEFL KEIDEKGLDD DDLIIGLKGK ERELKLAGRF
     FSLMSWKLRE YFVITEYLIK THFVPMFKGL TMADDLTAVI KKMLDSSSGQ GLKSYEAICI
     ANHIDYEKWN NHQRKLSNGP VFRVMGQFLG YPSLIERTHE FFEKSLIYYN GRPDLMRVQN
     NTLINSTSQR VCWQGQEGGL EGLRQKGWSI LNLLVIQREA KIRNTAVKVL AQGDNQVICT
     QYKTKKSRNV VELQGALNQM VSNNEKIMTA IKVGTGRLGL LVNDDETMQS ADYLNYGKIP
     IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN IMSSVSTNAL TVAHFAENPI NAMIQYNYFG
     TFARLLLMMH DPALRQSLYE VQDKIPGLHS STFKYAMLYL DPSIGGVSGM SLSRFLIRAF
     PDPVTESLSF WRFIHLHARS EHLKEMSAVF GNPEIAKFRI THIDKLVEDP TSLNIAMGMS
     PANLLKTEVK KCLIESRQTI RNQVIKDATI YLYHEEDRLR SFLWSINPLF PRFLSEFKSG
     TFLGVADGLI SLFQNSRTIR NSFKKKYHRE LDDLIVRSEV SSLTHLGKLH LRRGSCKMWT
     CSATHADTLR YKSWGRTVIG TTVPHPLEML GPQHRKETPC APCNTSGFNY VSVHCPDGIH
     DVFSSRGPLP AYLGSKTSES TSILQPWERE SKVPLIKRAT RLRDAISWFV EPDSKLAMTI
     LSNIHSLTGE EWTKRQHGFK RTGSALHRFS TSRMSHGGFA SQSTAALTRL MATTDTMRDL
     GDQNFDFLFQ ATLLYAQITT TVARDGWTTS CTDHYHIACK SCLRPIEEIT LDSSMDYTPP
     DVSHVLKTWR NGEGSWGQEI KQIYPLEGNW KNLAPAEQSY QVGRCIGFLY GDLAYRKSTH
     AEDSSLFPLS IQSRIRGRGF LKGLLDGLMR ASCCQVIHRR SLAHLKRPAN AVYGGLIYLI
     DKLSVSPPFL SLTRSGPIRD ELETIPHKIP TSYPTSNRDM GVIVRNYFKY QCRLIEKGKY
     RSHYSQLWLF SDVLSIDFLG PFSISTTLLQ ILYKPSLSGK DKNELRELAN LSSLLRSGEG
     WEDIHVKFFT KDILLCPEEI RHACKFGIAK DNNKDMGYPP WGRESRGTIT TIPVYYTTTP
     YPKMLEMPPR IQNPLLSGIR LGQLPTGAHY KIRSILHGMG IHYRDFLSCG DGSGGMTAAL
     LRENVHSRGI FNSLLELSGS VMRGASPEPP SALETLGGDK SRCVNGETCW EHPSDLCDPR
     TWDYFLRLKA GLGLQIDLIV MDMEVRDSST SLKIETNVRN YVHRILDEQG VLIYKTYGTY
     ICESEKNAVT ILGPMFKTVD LVQTEFSSSQ TSEVYMVCKG LKKLIDEPNP DWSSINESWK
     NLYAFQSSEQ EFARARKVST YFTLTGIPSQ FIPDPFVNLE TMLQIFGVPT GVSHAAALKS
     SDRPADLLTI SLFYMAIISY YNINHIRVGP MPPNPPSDGI AQNVGIAITG ISFWLSLMEK
     DIPLYQQCLA VIQQSFPIRW EAVSVKGGYK QKWSTRGDGL PKDTRISDSL APIGNWIRSL
     ELVRNQVRLN PFNEILFNQL CRTVDNHLKW SNLRNNTGMI AWINRQISKE DRSILMLKSD
     LHEENSWRD
//