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Protein

Matrix protein

Gene

M

Organism
Vesicular stomatitis Indiana virus (strain 85CLB South America) (VSIV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in assembly and budding of virion. Condensates the ribonucleocapsid core during virus assembly. Shut off cellular transcription by inhibiting mRNA nuclear export through direct interaction with host RAE1-NUP98 complex. This shutoff presumably inhibits interferon signaling and thus establishment of antiviral state in virus infected cells. Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell (By similarity).By similarity

Miscellaneous

Most abundant protein in the virion.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host mRNA nuclear export by virus, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein
Gene namesi
Name:M
OrganismiVesicular stomatitis Indiana virus (strain 85CLB South America) (VSIV)
Taxonomic identifieri434490 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeVesiculovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000007625 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Biotechnological usei

VSV is used as an oncolytic agent for cancer therapy, because of his wide host range, rapid replication and mild pathogenicity in humans. VSV used are mutated at M51R in their matrix protein. These mutated viruses cannot successfully infect normal cells, being unable to counteract the antiviral state induced by interferon-alpha in normal cells. Cancer cells are impeded with responsiveness to interferon, and then can be successfully infected and lysed by the virus.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872571 – 229Matrix proteinAdd BLAST229

Post-translational modificationi

Phosphorylated by host.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homomultimer. Interacts with viral nucleocapsid. Interacts with host RAE1-NUP98 complex (By similarity).By similarity

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 76Combined sources17
Helixi81 – 88Combined sources8
Helixi89 – 92Combined sources4
Helixi99 – 101Combined sources3
Helixi102 – 115Combined sources14
Beta strandi117 – 120Combined sources4
Beta strandi130 – 143Combined sources14
Beta strandi156 – 164Combined sources9
Beta strandi167 – 179Combined sources13
Helixi189 – 191Combined sources3
Turni194 – 197Combined sources4
Helixi200 – 206Combined sources7
Turni207 – 209Combined sources3
Beta strandi210 – 213Combined sources4
Beta strandi216 – 219Combined sources4
Beta strandi221 – 225Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OWRX-ray3.15C44-229[»]
ProteinModelPortaliQ8B0H7.
SMRiQ8B0H7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 27PPXY motif4
Motifi37 – 40PTAP/PSAP motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. M contains two overlapping L domains: a PPXY motif which interacts with the WW domain 3 of NEDD4 and a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000OM.

Family and domain databases

Gene3Di3.10.460.10. 1 hit.
InterProiView protein in InterPro
IPR009397. Vesiculo_matrix.
IPR036711. VSV_matrix_sf.
PfamiView protein in Pfam
PF06326. Vesiculo_matrix. 1 hit.
SUPFAMiSSF75404. SSF75404. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform M (identifier: Q8B0H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLKKILGL KGKGKKSKKL GIAPPPYEED TSMEYAPSAP IDKSYFGVDE
60 70 80 90 100
MDTHDPNQLR YEKFFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG
110 120 130 140 150
KRPFYKILAF LGSSNLKATP AVLADQGQPE YHAHCEGRAY LPHRMGKTPP
160 170 180 190 200
MLNVPEHFRR PFNIGLYKGT IELTMTIYDD ESLEAAPMIW DHFNSSKFSD
210 220
FREKALMFGL IVEKKASGAW VLDSVSHFK
Length:229
Mass (Da):26,024
Last modified:March 1, 2003 - v1
Checksum:iBE54567021F5B853
GO
Isoform M2 (identifier: Q8B0H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Show »
Length:197
Mass (Da):22,612
Checksum:i21111CE6D062DDE6
GO
Isoform M3 (identifier: Q8B0H7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Show »
Length:179
Mass (Da):20,611
Checksum:i9C74321A4E3422F2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0254121 – 50Missing in isoform M3. CuratedAdd BLAST50
Alternative sequenceiVSP_0254131 – 32Missing in isoform M2. CuratedAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF473865 Genomic RNA. Translation: AAN16987.1.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiMATRX_VSIVS
AccessioniPrimary (citable) accession number: Q8B0H7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families