ID   Q8AYP7_CHICK            Unreviewed;       916 AA.
AC   Q8AYP7;
DT   01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 112.
DE   RecName: Full=Hexokinase-2 {ECO:0000256|ARBA:ARBA00039453};
DE            EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
DE   AltName: Full=Hexokinase type II {ECO:0000256|ARBA:ARBA00041371};
GN   Name=HK2 {ECO:0000313|EMBL:BAC20933.1};
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031 {ECO:0000313|EMBL:BAC20933.1};
RN   [1] {ECO:0000313|EMBL:BAC20933.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Seki Y., Sato K., Kono T., Akiba Y.;
RT   "Cloning and gene expression of hexokinase I and II in the chicken skeletal
RT   muscle.";
RL   Anim. Sci. J. 76:491-497(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 1/4.
CC       {ECO:0000256|ARBA:ARBA00004888}.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC       {ECO:0000256|ARBA:ARBA00005028}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000256|ARBA:ARBA00004514}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004170}. Mitochondrion outer membrane
CC       {ECO:0000256|ARBA:ARBA00004450}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004450}.
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000256|ARBA:ARBA00009225}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB083370; BAC20933.1; -; mRNA.
DR   RefSeq; NP_989543.1; NM_204212.1.
DR   AlphaFoldDB; Q8AYP7; -.
DR   GeneID; 374044; -.
DR   KEGG; gga:374044; -.
DR   VEuPathDB; HostDB:geneid_374044; -.
DR   PhylomeDB; Q8AYP7; -.
DR   Reactome; R-GGA-352832; Glucose transport.
DR   UniPathway; UPA00109; UER00180.
DR   UniPathway; UPA00242; -.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004340; F:glucokinase activity; TAS:Reactome.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:1904659; P:glucose transmembrane transport; TAS:Reactome.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR   Gene3D; 3.30.420.40; -; 2.
DR   Gene3D; 3.40.367.20; -; 2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR019807; Hexokinase_BS.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; HEXOKINASE; 1.
DR   PANTHER; PTHR19443:SF4; HEXOKINASE-2; 1.
DR   Pfam; PF00349; Hexokinase_1; 2.
DR   Pfam; PF03727; Hexokinase_2; 2.
DR   PRINTS; PR00475; HEXOKINASE.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR   PROSITE; PS00378; HEXOKINASE_1; 2.
DR   PROSITE; PS51748; HEXOKINASE_2; 2.
PE   2: Evidence at transcript level;
KW   Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW   Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:BAC20933.1};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW   Mitochondrion outer membrane {ECO:0000256|ARBA:ARBA00022787};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          22..219
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          226..459
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
FT   DOMAIN          471..667
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          674..907
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
SQ   SEQUENCE   916 AA;  102417 MW;  81E4A1E7D76FF550 CRC64;
     MIASHLLAYF FTELNHDQAQ KVDKYLYHMR LSEDTLQEVS ERFRKEMEKG LGADTNPTAS
     VKMLPSFVRS TPDGTEDGDF LALDLGGTNF RVLRVKVSDN GLQKVEMESQ IYEIHEDLMR
     GSGMQLFDHI AECLGNFMEK LKIKDKKLPL GFTFSFPCHQ TKLDESILVN WTKGFKCSSV
     EGKDVVSLLR RAIKKRGDFD IDIVAVVNDT VGTMMSCGYD DQNCEVGLIV GTGTNACYME
     EMRHIDLVEG DEGRMCINME WGAFGDDGAL NDIRTEFDHE IDMGSLNPGK QLFEKMISGM
     YMGELVRLIL VKMAKEGLLF QGKLSSDLRT TGHFETRFVS AIEKEKEGLQ KAHEILTKLG
     LEPSHEDCLA THRICQIVST RSANLCGATL AARLRRIKEY KGVDPLRSTV GVDGSVYKKY
     PHFARRLHKT VRKLLPDCEI RFVRSEDGSG KGAAMVTAVA YRLAAQHKAR QKILEALKLS
     HEQLLEVKRR MRVEMEKGLG KETHAEATVK MLPTYVCSTP DGTEKGDFLA LDLGGTNFRV
     LLVRVRNGMR RGVEMHNKIY SIPLEVMQGT GEELFDHIVH CISDFLEYMG MKGVSLPLGF
     TFSFPCKQTN LDEGILLKWT KGFKATGCEG EDVVSLLKEA IHRREEFDLD VVAVVNDTVG
     TMMTCGYEDP YCEVGLIVGT GSNACYMEEM RNVELVEGDE GRMCVNMEWG AFGDNGCLDD
     IQTEFDLAVD ELSLNPGKQR FEKMISGMYL GEIVRNILMD FTKRGLLFRG RISERLKTRG
     IFETKFLSQI ESDCLALLQV RSILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA
     VVDKIRENRG LDFLKVTVGV DGTLYKLHPH FSAIMQDTVR QLSPCCEVTF LQSEDGSGKG
     AALITAVACR IREAGQ
//