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Protein

Wee1-like protein kinase 1-A

Gene

wee1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur. Involved in convergent extension of the paraxial mesoderm during neurulation by inhibiting the cell cycle.2 Publications

Caution

Was initially assigned as wee2 (PubMed:12217326). However, it corresponds to the zygotic protein WEE1 in mammals.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei253ATPPROSITE-ProRule annotation1
Active sitei351Proton acceptorPROSITE-ProRule annotation1
Metal bindingi356Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi388Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi230 – 238ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 1-A (EC:2.7.10.2)
Alternative name(s):
Zygotic wee1-like protein kinase 2
Short name:
XWee2
Gene namesi
Name:wee1-a
Synonyms:wee2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-17338608 wee1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095221 – 571Wee1-like protein kinase 1-AAdd BLAST571

Proteomic databases

PRIDEiQ8AYK6

Expressioni

Tissue specificityi

Zygotically expressed. Expressed in regions of the embryo that are devoid of mitotic cells, such as the involuting mesoderm.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8AYK6
SMRiQ8AYK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 494Protein kinasePROSITE-ProRule annotationAdd BLAST271

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili500 – 539Sequence analysisAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi52 – 55Poly-Glu4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG005050
KOiK06632

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8AYK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLQPVPHRL LFSDSDDEED GHSTGEDSAF QESDSPVSRL REKQEGPGGS
60 70 80 90 100
WEEEEGLGSS PIKSPGHFFM CDSPTYRDLP PASPPGPAAS PPDCPGTPPH
110 120 130 140 150
KTFRKLRLFD TPHTPKSLLS KARGIGSSAL RFRGGTLFRE AEKLPKPEFT
160 170 180 190 200
YSTPQVNINP FTPDSLEIQS STGLCRRRKR ALLNDSCGED MEGSDCELED
210 220 230 240 250
EDVRPAKRIP ITESNMKSRY ATEFHELEKI GSGEFGSVFK CVKRLDGCIY
260 270 280 290 300
AIKRSKKPMA GSVDEQNALR EVYAHAVLGQ HPHVVRYYSA WAEDDHMLIQ
310 320 330 340 350
NEYCNGGSLA DAISENYRTM QYFTEPELKD LLLQVARGLK YIHSMSLVHM
360 370 380 390 400
DIKPSNIFIS RITVPNTGVE EGDDEDCGSG NVVYKIGDLG HVTRVSSPQV
410 420 430 440 450
EEGDSRFLAN EVLQEDYTHL AKADIFALAL TVWCAAGAEP FPTNGDQWHE
460 470 480 490 500
IRQGKLPRVP QLLSQEFVDL IKLMISPDSE KRPSSMALVK HSVLLSASRK
510 520 530 540 550
NAEQLRIELN AEKFKNALLQ KELKKAQIAK AAAEERALFP DRIATRSTTQ
560 570
SNRTTRLIGK KMNRSVSLTI Y
Length:571
Mass (Da):63,607
Last modified:March 1, 2003 - v1
Checksum:iA3E78D020B22BB28
GO
Isoform 2 (identifier: Q8AYK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: W → WE
     74-84: Missing.

Note: No experimental confirmation available.
Show »
Length:561
Mass (Da):62,541
Checksum:i5EAFB3247A0DF8C7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04132351W → WE in isoform 2. 1 Publication1
Alternative sequenceiVSP_04132474 – 84Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358869 mRNA Translation: AAN07091.1
BC169775 mRNA Translation: AAI69775.1
RefSeqiNP_001082306.1, NM_001088837.1 [Q8AYK6-1]
UniGeneiXl.16297

Genome annotation databases

GeneIDi398396
KEGGixla:398396

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWEE1A_XENLA
AccessioniPrimary (citable) accession number: Q8AYK6
Secondary accession number(s): B7ZQE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

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