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Protein

Wee1-like protein kinase 1-A

Gene

wee1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur. Involved in convergent extension of the paraxial mesoderm during neurulation by inhibiting the cell cycle.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei253 – 2531ATPPROSITE-ProRule annotation
Active sitei351 – 3511Proton acceptorPROSITE-ProRule annotation
Metal bindingi356 – 3561Magnesium; via carbonyl oxygenBy similarity
Metal bindingi388 – 3881Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi230 – 2389ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 1-A (EC:2.7.10.2)
Alternative name(s):
Zygotic wee1-like protein kinase 2
Short name:
XWee2
Gene namesi
Name:wee1-a
Synonyms:wee2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Wee1-like protein kinase 1-APRO_0000409522Add
BLAST

Expressioni

Tissue specificityi

Zygotically expressed. Expressed in regions of the embryo that are devoid of mitotic cells, such as the involuting mesoderm.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8AYK6.
SMRiQ8AYK6. Positions 216-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini224 – 494271Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili500 – 53940Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 554Poly-Glu

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG005050.
KOiK06632.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8AYK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLQPVPHRL LFSDSDDEED GHSTGEDSAF QESDSPVSRL REKQEGPGGS
60 70 80 90 100
WEEEEGLGSS PIKSPGHFFM CDSPTYRDLP PASPPGPAAS PPDCPGTPPH
110 120 130 140 150
KTFRKLRLFD TPHTPKSLLS KARGIGSSAL RFRGGTLFRE AEKLPKPEFT
160 170 180 190 200
YSTPQVNINP FTPDSLEIQS STGLCRRRKR ALLNDSCGED MEGSDCELED
210 220 230 240 250
EDVRPAKRIP ITESNMKSRY ATEFHELEKI GSGEFGSVFK CVKRLDGCIY
260 270 280 290 300
AIKRSKKPMA GSVDEQNALR EVYAHAVLGQ HPHVVRYYSA WAEDDHMLIQ
310 320 330 340 350
NEYCNGGSLA DAISENYRTM QYFTEPELKD LLLQVARGLK YIHSMSLVHM
360 370 380 390 400
DIKPSNIFIS RITVPNTGVE EGDDEDCGSG NVVYKIGDLG HVTRVSSPQV
410 420 430 440 450
EEGDSRFLAN EVLQEDYTHL AKADIFALAL TVWCAAGAEP FPTNGDQWHE
460 470 480 490 500
IRQGKLPRVP QLLSQEFVDL IKLMISPDSE KRPSSMALVK HSVLLSASRK
510 520 530 540 550
NAEQLRIELN AEKFKNALLQ KELKKAQIAK AAAEERALFP DRIATRSTTQ
560 570
SNRTTRLIGK KMNRSVSLTI Y
Length:571
Mass (Da):63,607
Last modified:March 1, 2003 - v1
Checksum:iA3E78D020B22BB28
GO
Isoform 2 (identifier: Q8AYK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: W → WE
     74-84: Missing.

Note: No experimental confirmation available.
Show »
Length:561
Mass (Da):62,541
Checksum:i5EAFB3247A0DF8C7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei51 – 511W → WE in isoform 2. 1 PublicationVSP_041323
Alternative sequencei74 – 8411Missing in isoform 2. 1 PublicationVSP_041324Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358869 mRNA. Translation: AAN07091.1.
BC169775 mRNA. Translation: AAI69775.1.
RefSeqiNP_001082306.1. NM_001088837.1. [Q8AYK6-1]
UniGeneiXl.16297.

Genome annotation databases

GeneIDi398396.
KEGGixla:398396.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358869 mRNA. Translation: AAN07091.1.
BC169775 mRNA. Translation: AAI69775.1.
RefSeqiNP_001082306.1. NM_001088837.1. [Q8AYK6-1]
UniGeneiXl.16297.

3D structure databases

ProteinModelPortaliQ8AYK6.
SMRiQ8AYK6. Positions 216-494.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398396.
KEGGixla:398396.

Organism-specific databases

CTDi398396.

Phylogenomic databases

HOVERGENiHBG005050.
KOiK06632.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple Cdk1 inhibitory kinases regulate the cell cycle during development."
    Leise W., Mueller P.R.
    Dev. Biol. 249:156-173(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Gastrula.
  3. "Inhibition of the cell cycle is required for convergent extension of the paraxial mesoderm during Xenopus neurulation."
    Leise W.F. III, Mueller P.R.
    Development 131:1703-1715(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWEE1A_XENLA
AccessioniPrimary (citable) accession number: Q8AYK6
Secondary accession number(s): B7ZQE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was initially assigned as wee2 (PubMed:12217326). However, it corresponds to the zygotic protein WEE1 in mammals.1 Publication

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.