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Q8AYK6

- WEE1A_XENLA

UniProt

Q8AYK6 - WEE1A_XENLA

Protein

Wee1-like protein kinase 1-A

Gene

wee1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur. Involved in convergent extension of the paraxial mesoderm during neurulation by inhibiting the cell cycle.2 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei253 – 2531ATPPROSITE-ProRule annotation
    Active sitei351 – 3511Proton acceptorPROSITE-ProRule annotation
    Metal bindingi356 – 3561Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi388 – 3881Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi230 – 2389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase 1-A (EC:2.7.10.2)
    Alternative name(s):
    Zygotic wee1-like protein kinase 2
    Short name:
    XWee2
    Gene namesi
    Name:wee1-a
    Synonyms:wee2
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 571571Wee1-like protein kinase 1-APRO_0000409522Add
    BLAST

    Expressioni

    Tissue specificityi

    Zygotically expressed. Expressed in regions of the embryo that are devoid of mitotic cells, such as the involuting mesoderm.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8AYK6.
    SMRiQ8AYK6. Positions 216-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini224 – 494271Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili500 – 53940Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi52 – 554Poly-Glu

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    HOVERGENiHBG005050.
    KOiK06632.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8AYK6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLQPVPHRL LFSDSDDEED GHSTGEDSAF QESDSPVSRL REKQEGPGGS    50
    WEEEEGLGSS PIKSPGHFFM CDSPTYRDLP PASPPGPAAS PPDCPGTPPH 100
    KTFRKLRLFD TPHTPKSLLS KARGIGSSAL RFRGGTLFRE AEKLPKPEFT 150
    YSTPQVNINP FTPDSLEIQS STGLCRRRKR ALLNDSCGED MEGSDCELED 200
    EDVRPAKRIP ITESNMKSRY ATEFHELEKI GSGEFGSVFK CVKRLDGCIY 250
    AIKRSKKPMA GSVDEQNALR EVYAHAVLGQ HPHVVRYYSA WAEDDHMLIQ 300
    NEYCNGGSLA DAISENYRTM QYFTEPELKD LLLQVARGLK YIHSMSLVHM 350
    DIKPSNIFIS RITVPNTGVE EGDDEDCGSG NVVYKIGDLG HVTRVSSPQV 400
    EEGDSRFLAN EVLQEDYTHL AKADIFALAL TVWCAAGAEP FPTNGDQWHE 450
    IRQGKLPRVP QLLSQEFVDL IKLMISPDSE KRPSSMALVK HSVLLSASRK 500
    NAEQLRIELN AEKFKNALLQ KELKKAQIAK AAAEERALFP DRIATRSTTQ 550
    SNRTTRLIGK KMNRSVSLTI Y 571
    Length:571
    Mass (Da):63,607
    Last modified:March 1, 2003 - v1
    Checksum:iA3E78D020B22BB28
    GO
    Isoform 2 (identifier: Q8AYK6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         51-51: W → WE
         74-84: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:561
    Mass (Da):62,541
    Checksum:i5EAFB3247A0DF8C7
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei51 – 511W → WE in isoform 2. 1 PublicationVSP_041323
    Alternative sequencei74 – 8411Missing in isoform 2. 1 PublicationVSP_041324Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF358869 mRNA. Translation: AAN07091.1.
    BC169775 mRNA. Translation: AAI69775.1.
    RefSeqiNP_001082306.1. NM_001088837.1. [Q8AYK6-1]
    UniGeneiXl.16297.

    Genome annotation databases

    GeneIDi398396.
    KEGGixla:398396.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF358869 mRNA. Translation: AAN07091.1 .
    BC169775 mRNA. Translation: AAI69775.1 .
    RefSeqi NP_001082306.1. NM_001088837.1. [Q8AYK6-1 ]
    UniGenei Xl.16297.

    3D structure databases

    ProteinModelPortali Q8AYK6.
    SMRi Q8AYK6. Positions 216-494.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398396.
    KEGGi xla:398396.

    Organism-specific databases

    CTDi 398396.

    Phylogenomic databases

    HOVERGENi HBG005050.
    KOi K06632.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple Cdk1 inhibitory kinases regulate the cell cycle during development."
      Leise W., Mueller P.R.
      Dev. Biol. 249:156-173(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Gastrula.
    3. "Inhibition of the cell cycle is required for convergent extension of the paraxial mesoderm during Xenopus neurulation."
      Leise W.F. III, Mueller P.R.
      Development 131:1703-1715(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiWEE1A_XENLA
    AccessioniPrimary (citable) accession number: Q8AYK6
    Secondary accession number(s): B7ZQE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3