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Q8AYK6 (WEE1A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Wee1-like protein kinase 1-A
EC=2.7.10.2
Alternative name(s):
Zygotic wee1-like protein kinase 2
Short name=XWee2
Gene names
Name:wee1-a
Synonyms:wee2
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur. Involved in convergent extension of the paraxial mesoderm during neurulation by inhibiting the cell cycle. Ref.1 Ref.3

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Nucleus By similarity.

Tissue specificity

Zygotically expressed. Expressed in regions of the embryo that are devoid of mitotic cells, such as the involuting mesoderm. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Caution

Was initially assigned as wee2 (Ref.1). However, it corresponds to the zygotic protein WEE1 in mammals.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8AYK6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8AYK6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: W → WE
     74-84: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Wee1-like protein kinase 1-A
PRO_0000409522

Regions

Domain224 – 494271Protein kinase
Nucleotide binding230 – 2389ATP By similarity
Coiled coil500 – 53940 Potential
Compositional bias52 – 554Poly-Glu

Sites

Active site3511Proton acceptor By similarity
Metal binding3561Magnesium; via carbonyl oxygen By similarity
Metal binding3881Magnesium; via carbonyl oxygen By similarity
Binding site2531ATP By similarity

Natural variations

Alternative sequence511W → WE in isoform 2.
VSP_041323
Alternative sequence74 – 8411Missing in isoform 2.
VSP_041324

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: A3E78D020B22BB28

FASTA57163,607
        10         20         30         40         50         60 
MSLQPVPHRL LFSDSDDEED GHSTGEDSAF QESDSPVSRL REKQEGPGGS WEEEEGLGSS 

        70         80         90        100        110        120 
PIKSPGHFFM CDSPTYRDLP PASPPGPAAS PPDCPGTPPH KTFRKLRLFD TPHTPKSLLS 

       130        140        150        160        170        180 
KARGIGSSAL RFRGGTLFRE AEKLPKPEFT YSTPQVNINP FTPDSLEIQS STGLCRRRKR 

       190        200        210        220        230        240 
ALLNDSCGED MEGSDCELED EDVRPAKRIP ITESNMKSRY ATEFHELEKI GSGEFGSVFK 

       250        260        270        280        290        300 
CVKRLDGCIY AIKRSKKPMA GSVDEQNALR EVYAHAVLGQ HPHVVRYYSA WAEDDHMLIQ 

       310        320        330        340        350        360 
NEYCNGGSLA DAISENYRTM QYFTEPELKD LLLQVARGLK YIHSMSLVHM DIKPSNIFIS 

       370        380        390        400        410        420 
RITVPNTGVE EGDDEDCGSG NVVYKIGDLG HVTRVSSPQV EEGDSRFLAN EVLQEDYTHL 

       430        440        450        460        470        480 
AKADIFALAL TVWCAAGAEP FPTNGDQWHE IRQGKLPRVP QLLSQEFVDL IKLMISPDSE 

       490        500        510        520        530        540 
KRPSSMALVK HSVLLSASRK NAEQLRIELN AEKFKNALLQ KELKKAQIAK AAAEERALFP 

       550        560        570 
DRIATRSTTQ SNRTTRLIGK KMNRSVSLTI Y

« Hide

Isoform 2 [UniParc].

Checksum: 5EAFB3247A0DF8C7
Show »

FASTA56162,541

References

« Hide 'large scale' references
[1]"Multiple Cdk1 inhibitory kinases regulate the cell cycle during development."
Leise W., Mueller P.R.
Dev. Biol. 249:156-173(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Gastrula.
[3]"Inhibition of the cell cycle is required for convergent extension of the paraxial mesoderm during Xenopus neurulation."
Leise W.F. III, Mueller P.R.
Development 131:1703-1715(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF358869 mRNA. Translation: AAN07091.1.
BC169775 mRNA. Translation: AAI69775.1.
RefSeqNP_001082306.1. NM_001088837.1.
UniGeneXl.16297.

3D structure databases

ProteinModelPortalQ8AYK6.
SMRQ8AYK6. Positions 216-494.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID398396.
KEGGxla:398396.

Organism-specific databases

CTD398396.

Phylogenomic databases

HOVERGENHBG005050.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWEE1A_XENLA
AccessionPrimary (citable) accession number: Q8AYK6
Secondary accession number(s): B7ZQE2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: March 1, 2003
Last modified: April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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