Reviewed, UniProtKB/Swiss-Prot Q8AYG3 (TTK_DANRE)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase Ttk
    EC=2.7.12.1
Alternative name(s):
    Mitotic checkpoint serine/threonine-protein kinase Mps1
    Monopolar spindle protein 1
    Protein nightcap

Gene names

Name:	ttk
Synonyms:	mps1, ncp

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	982 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates proteins on serine, threonine, and tyrosine By similarity. Involved in mitotic cell cycle checkpoint control. Required for fin and heart regeneration. Required to prevent chromosome segregation errors during meiosis.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB Q86UE8
Tissue specificity	Barely detectable in adult somatic tissues. Expressed in immature germ cells that have not completed meiosis. In ovary, expressed predominantly in previtellogenic oocytes. In testis, expressed in primary and secondary spermatocytes, but not mature spermatozoa. Ref.1 Ref.4
Induction	Strongly induced in a subpopulation of cells in the proximal blastema during regenerative outgrowth. Ref.1
Disruption phenotype	Mutants show anomalies in the chromosome number of male germ cells, leading to aneuploidy and severe developmental defects in their progeny. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	anatomical structure homeostasis Inferred from mutant phenotype. Source: ZFIN cell division Inferred from electronic annotation. Source: UniProtKB-KW embryonic development Ref.1 Inferred from mutant phenotype. Source: UniProtKB female meiosis chromosome segregation Ref.4 Inferred from mutant phenotype. Source: ZFIN fin regeneration Ref.1 Inferred from mutant phenotype. Source: ZFIN male meiosis chromosome segregation Ref.4 Inferred from mutant phenotype. Source: ZFIN mitotic cell cycle checkpoint Ref.1 Ref.4 Inferred from mutant phenotype. Source: UniProtKB protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 982	982	Dual specificity protein kinase Ttk		PRO_0000086390
Regions
	Domain	653 – 916	264	Protein kinase
	Nucleotide binding	659 – 667	9	ATP By similarity
	Compositional bias	558 – 562	5	Poly-Ser
Sites
	Active site	775	1	Proton acceptor By similarity
	Binding site	681	1	ATP By similarity
Experimental info
	Mutagenesis	783	1	I → K in zp1; temperature-sensitive mutation that blocks fin regeneration at the restrictive temperature. Ref.1
	Sequence conflict	210	1	N → S in AAN61408. Ref.1
	Sequence conflict	215	1	S → SS in AAN61408. Ref.1
	Sequence conflict	494 – 495	2	EH → DL in AAN61408. Ref.1
	Sequence conflict	509 – 510	2	GM → YI in AAN61408. Ref.1
	Sequence conflict	516 – 517	2	PV → SA in AAN61408. Ref.1
	Sequence conflict	553 – 554	2	PV → SL in AAN61408. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8AYG3-1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: 3B9C7D38DDF76C22	FASTA	982	110,012
	10 20 30 40 50 60 MDEEESTERQ MQIAMLCQKL AMMKQLFNED DTDYINQAIS SNSPDTCRTF LSNLEKKGNP 70 80 90 100 110 120 QADPSLLSKL MDSYTRVFSS MPLGKYSQNE SYAKMLVRFA ELKAIQDVND AQTSFDIARS 130 140 150 160 170 180 HCKDFAFVHV AYAQFELLQG NMKKCTMILQ KAFEMNAKPR HVLEAAVRNL KTGKRQLLSH 190 200 210 220 230 240 EDKENLSVSA LDHTQGSRRS DGTCELKPSN TFLHSDQKFS PQEENGPVWR TGSQHRRTAM 250 260 270 280 290 300 AERVPMVPLS IPENETSDSD CAQKAEAPFT HSSGFSRQTS GSSVRSAFSL CSSKKGTPDG 310 320 330 340 350 360 DSYSLNIKPP VISPDYLRED IEEGHTITAL LNRAEKRETA RTEETTDINQ IISTNSTEGC 370 380 390 400 410 420 QAFLKNLEKR ADPHSDAAFL SKLLDCYSKV FARFPLAEHC KTESYARMLV RYAELKGIED 430 440 450 460 470 480 PEDAADDFSI ARSHCKAFAF VHIAHAQFEL SRGNSRKSVS ILQKALSSNA RPIELLQTAI 490 500 510 520 530 540 RNLKSGKTLL LPAEHQESSE AENVEAQNGM KREENPVKAP EDHQKPFSKE TSSEWKIPAL 550 560 570 580 590 600 ITKHTSPEDR KAPVEPVSSS SSHHAVRTPA PLRLNPSLSC QTPNYRQPNP NSFVTPVVKQ 610 620 630 640 650 660 RPVIVSVPAT AQKMCPTALP CTPQSGVSYI QPPTQTPSSA FSNESITIKG KQFFIFKMIG 670 680 690 700 710 720 RGGSSKVYQV FDHKKHVYAV KYVNLEEADA QAVESYKNEI EHLNHLQQYS DQIIKLYDYE 730 740 750 760 770 780 ITSSYIYMLM ECGHLDLNTW LRNRKTVKPL DRKAYWRNML EAVHTIHKHG IVHSDLKPAN 790 800 810 820 830 840 FLIVDGSLKL IDFGIANQIQ PDVTSIMKDS QVGTLNYMPP EAIKDTSSNG KPGSKISAKG 850 860 870 880 890 900 DVWSLGCILY CMTYGKTPFQ NITNQISKIH AIIDPSHEID FPDIPEKDLL DVLKKCLVRN 910 920 930 940 950 960 PRERISIAEL LDHPYLQLQP QPAPEPAETS SSDFKRILNE LVALQSPNSI ARAASNLAMM 970 980 CNSGRKLDVS ECVKSSSQTL WK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration." Poss K.D., Nechiporuk A., Hillam A.M., Johnson S.L., Keating M.T. Development 129:5141-5149(2002) [PubMed: 12399306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF ILE-783. Strain: AB.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Heart regeneration in zebrafish." Poss K.D., Wilson L.G., Keating M.T. Science 298:2188-2190(2002) [PubMed: 12481136] [Abstract] Cited for: FUNCTION.
[4]	"Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1." Poss K.D., Nechiporuk A., Stringer K.F., Lee C., Keating M.T. Genes Dev. 18:1527-1532(2004) [PubMed: 15231734] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF488735 mRNA. Translation: AAN61408.1. BC054627 mRNA. Translation: AAH54627.1.
IPI	IPI00498771.
RefSeq	NP_778207.1.
UniGene	Dr.80755
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000060630; ENSDARP00000060629; ENSDARG00000041361; Danio rerio. [Genome view]
GeneID	317763.
KEGG	dre:317763.
Organism-specific databases
CTD	317763.
ZFIN	ZDB-GENE-030123-1. ttk.
Phylogenomic databases
HOVERGEN	Q8AYG3.
Enzyme and pathway databases
BRENDA	2.7.12.1. 96826.
Gene expression databases
ArrayExpress	Q8AYG3.
Bgee	Q8AYG3.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TTK_DANRE

Accession

Primary (citable) accession number: Q8AYG3
Secondary accession number(s): Q7T2A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	March 6, 2007
Last modified:	September 1, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project