Q8AYC9 (CHK1_CHICK) Reviewed, UniProtKB/Swiss-Prot
Last modified May 1, 2013. Version 76. History...
Names and origin
|Protein names||Recommended name:|
Serine/threonine-protein kinase Chk1
CHK1 checkpoint homolog
|Organism||Gallus gallus (Chicken) [Reference proteome]|
|Taxonomic identifier||9031 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus|
|Sequence length||476 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. This inhibits their activity through proteasomal degradation, nucleo-cytoplasmic shuttling and inhibition by proteins of the 13-3-3 family. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May promote DNA repair, regulate chromatin assembly and the transcription of genes that regulate cell-cycle progression. May also play a role in replication fork maintenance By similarity. Ref.1
ATP + a protein = ADP + a phosphoprotein.
Activated through phosphorylation by ATR or ATM in response to DNA damage or inhibition of DNA replication By similarity.
Interacts with and phosphorylates CLSPN, an adapter protein that regulates the ATR-dependent phosphorylation of CHEK1 By similarity.
The autoinhibitory region (AIR) inhibits the activity of the kinase domain By similarity.
Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation By similarity. Phosphorylation at Ser-345 induces a change in the conformation of the protein and activates the kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Ref.1
Contains 1 protein kinase domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 476||476||Serine/threonine-protein kinase Chk1||PRO_0000085851|
|Domain||9 – 265||257||Protein kinase|
|Nucleotide binding||15 – 23||9||ATP By similarity|
|Region||1 – 265||265||Interaction with CLSPN By similarity|
|Region||391 – 476||86||Autoinhibitory region By similarity|
|Active site||130||1||Proton acceptor By similarity|
|Binding site||38||1||ATP By similarity|
Amino acid modifications
|Modified residue||280||1||Phosphoserine; by PKB/AKT1 By similarity|
|Modified residue||296||1||Phosphoserine By similarity|
|Modified residue||317||1||Phosphoserine; by ATM and ATR By similarity|
|Modified residue||345||1||Phosphoserine Ref.1|
|AF525027 mRNA. Translation: AAN33019.1.|
|RefSeq||NP_989676.1. NM_204345.1. |
3D structure databases
|SMR||Q8AYC9. Positions 3-272. |
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
Enzyme and pathway databases
|Reactome||REACT_115612. DNA replication and repair. |
Family and domain databases
|InterPro||IPR011009. Kinase-like_dom. |
|Pfam||PF00069. Pkinase. 1 hit. |
|SMART||SM00220. S_TKc. 1 hit. |
|SUPFAM||SSF56112. Kinase_like. 1 hit. |
|PROSITE||PS00107. PROTEIN_KINASE_ATP. 1 hit. |
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
|Accession||Primary (citable) accession number: Q8AYC9|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families