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Q8AYC9

- CHK1_CHICK

UniProt

Q8AYC9 - CHK1_CHICK

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Protein

Serine/threonine-protein kinase Chk1

Gene

CHEK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. This inhibits their activity through proteasomal degradation, nucleo-cytoplasmic shuttling and inhibition by proteins of the 13-3-3 family. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May promote DNA repair, regulate chromatin assembly and the transcription of genes that regulate cell-cycle progression. May also play a role in replication fork maintenance (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated through phosphorylation by ATR or ATM in response to DNA damage or inhibition of DNA replication.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATPPROSITE-ProRule annotation
Active sitei130 – 1301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 239ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. histone kinase activity (H3-T11 specific) Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. chromatin-mediated maintenance of transcription Source: UniProtKB
  3. DNA damage checkpoint Source: UniProtKB
  4. DNA damage induced protein phosphorylation Source: UniProtKB
  5. DNA repair Source: Reactome
  6. double-strand break repair Source: Reactome
  7. double-strand break repair via homologous recombination Source: Reactome
  8. G2 DNA damage checkpoint Source: UniProtKB
  9. histone H3-T11 phosphorylation Source: GOC
  10. negative regulation of mitosis Source: UniProtKB
  11. peptidyl-threonine phosphorylation Source: UniProtKB
  12. regulation of double-strand break repair via homologous recombination Source: UniProtKB
  13. regulation of histone H3-K9 acetylation Source: UniProtKB
  14. regulation of mitotic centrosome separation Source: UniProtKB
  15. regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
REACT_115715. ATM mediated phosphorylation of repair proteins.
REACT_115915. Chk1-controlled and DNA-damage induced centrosome duplication.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Chk1 (EC:2.7.11.1)
Alternative name(s):
CHK1 checkpoint homolog
Checkpoint kinase-1
Gene namesi
Name:CHEK1
Synonyms:CHK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. chromatin Source: UniProtKB
  3. condensed nuclear chromosome Source: UniProtKB
  4. cytoplasm Source: UniProtKB-KW
  5. nucleoplasm Source: Reactome
  6. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Serine/threonine-protein kinase Chk1PRO_0000085851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei280 – 2801Phosphoserine; by PKB/AKT1By similarity
Modified residuei296 – 2961PhosphoserineBy similarity
Modified residuei317 – 3171Phosphoserine; by ATM and ATRBy similarity
Modified residuei345 – 3451Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation (By similarity). Phosphorylation at Ser-345 induces a change in the conformation of the protein and activates the kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8AYC9.
PRIDEiQ8AYC9.

Interactioni

Subunit structurei

Interacts with and phosphorylates CLSPN, an adapter protein that regulates the ATR-dependent phosphorylation of CHEK1.By similarity

Protein-protein interaction databases

BioGridi675270. 1 interaction.
STRINGi9031.ENSGALP00000040314.

Structurei

3D structure databases

ProteinModelPortaliQ8AYC9.
SMRiQ8AYC9. Positions 3-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 265257Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 265265Interaction with CLSPNBy similarityAdd
BLAST
Regioni391 – 47686Autoinhibitory regionBy similarityAdd
BLAST

Domaini

The autoinhibitory region (AIR) inhibits the activity of the kinase domain.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000216658.
HOVERGENiHBG002590.
InParanoidiQ8AYC9.
KOiK02216.
PhylomeDBiQ8AYC9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8AYC9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVPFVEDWD LVQTLGEGAY GEVQLAVNRR TEEAVAVKIV DMKRAADCPE
60 70 80 90 100
NIKKEICINK MLNHENVVKF YGHRREGATQ YLFLEYCSGG ELFDRIEPDI
110 120 130 140 150
GMPEPEAQRF FQQLIAGVVY LHSMGITHRD LKPENLLLDE RDNLKISDFG
160 170 180 190 200
LATVFKHNGR ERLLNKMCGT LPYVAPELLR RPEFRAEPVD VWACGVVLTA
210 220 230 240 250
MLAGELPWDQ PSDSCQEYSD WKERKTYLAP WRKIDSAPLA LLHKILTENP
260 270 280 290 300
TARITIPDIK KDRWYCRPLK KGTKRGRVSS GGVTESPGAL PKHIRSDTDF
310 320 330 340 350
SPVKSALGED KASYSTSQPE PGTGGALWDS STGSIDRLVQ GISFSQPACP
360 370 380 390 400
EHMLLNSQLL GTPGSSQSPW QRLVRRMTRF FTKLDADGSY RSLRDVCEKM
410 420 430 440 450
GYGWKQSCTN QVTISTTDRR NNKLIFKVNL LEMESRILVD FRLSKGDGLE
460 470
FKRHFLKIKG KLSDVVSTQK VWLPPP
Length:476
Mass (Da):53,849
Last modified:March 1, 2003 - v1
Checksum:i0E54EE2196EF0C15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF525027 mRNA. Translation: AAN33019.1.
RefSeqiNP_989676.1. NM_204345.1.
UniGeneiGga.4288.
Gga.48286.

Genome annotation databases

GeneIDi374260.
KEGGigga:374260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF525027 mRNA. Translation: AAN33019.1 .
RefSeqi NP_989676.1. NM_204345.1.
UniGenei Gga.4288.
Gga.48286.

3D structure databases

ProteinModelPortali Q8AYC9.
SMRi Q8AYC9. Positions 3-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 675270. 1 interaction.
STRINGi 9031.ENSGALP00000040314.

Proteomic databases

PaxDbi Q8AYC9.
PRIDEi Q8AYC9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374260.
KEGGi gga:374260.

Organism-specific databases

CTDi 3703.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000216658.
HOVERGENi HBG002590.
InParanoidi Q8AYC9.
KOi K02216.
PhylomeDBi Q8AYC9.

Enzyme and pathway databases

Reactomei REACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
REACT_115715. ATM mediated phosphorylation of repair proteins.
REACT_115915. Chk1-controlled and DNA-damage induced centrosome duplication.

Miscellaneous databases

NextBioi 20813751.
PROi Q8AYC9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chk1-deficient tumour cells are viable but exhibit multiple checkpoint and survival defects."
    Zachos G., Rainey M.D., Gillespie D.A.F.
    EMBO J. 22:713-723(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION AT SER-345.

Entry informationi

Entry nameiCHK1_CHICK
AccessioniPrimary (citable) accession number: Q8AYC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3