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Q8AYC9

- CHK1_CHICK

UniProt

Q8AYC9 - CHK1_CHICK

Protein

Serine/threonine-protein kinase Chk1

Gene

CHEK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. This inhibits their activity through proteasomal degradation, nucleo-cytoplasmic shuttling and inhibition by proteins of the 13-3-3 family. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May promote DNA repair, regulate chromatin assembly and the transcription of genes that regulate cell-cycle progression. May also play a role in replication fork maintenance By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated through phosphorylation by ATR or ATM in response to DNA damage or inhibition of DNA replication.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381ATPPROSITE-ProRule annotation
    Active sitei130 – 1301Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 239ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histone kinase activity (H3-T11 specific) Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. chromatin-mediated maintenance of transcription Source: UniProtKB
    3. DNA damage checkpoint Source: UniProtKB
    4. DNA damage induced protein phosphorylation Source: UniProtKB
    5. DNA repair Source: Reactome
    6. double-strand break repair Source: Reactome
    7. double-strand break repair via homologous recombination Source: Reactome
    8. G2 DNA damage checkpoint Source: UniProtKB
    9. histone H3-T11 phosphorylation Source: GOC
    10. negative regulation of mitosis Source: UniProtKB
    11. peptidyl-threonine phosphorylation Source: UniProtKB
    12. regulation of double-strand break repair via homologous recombination Source: UniProtKB
    13. regulation of histone H3-K9 acetylation Source: UniProtKB
    14. regulation of mitotic centrosome separation Source: UniProtKB
    15. regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
    REACT_115715. ATM mediated phosphorylation of repair proteins.
    REACT_115915. Chk1-controlled and DNA-damage induced centrosome duplication.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase Chk1 (EC:2.7.11.1)
    Alternative name(s):
    CHK1 checkpoint homolog
    Checkpoint kinase-1
    Gene namesi
    Name:CHEK1
    Synonyms:CHK1
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. chromatin Source: UniProtKB
    3. condensed nuclear chromosome Source: UniProtKB
    4. cytoplasm Source: UniProtKB-SubCell
    5. nucleoplasm Source: Reactome
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476Serine/threonine-protein kinase Chk1PRO_0000085851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei280 – 2801Phosphoserine; by PKB/AKT1By similarity
    Modified residuei296 – 2961PhosphoserineBy similarity
    Modified residuei317 – 3171Phosphoserine; by ATM and ATRBy similarity
    Modified residuei345 – 3451Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation By similarity. Phosphorylation at Ser-345 induces a change in the conformation of the protein and activates the kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8AYC9.
    PRIDEiQ8AYC9.

    Interactioni

    Subunit structurei

    Interacts with and phosphorylates CLSPN, an adapter protein that regulates the ATR-dependent phosphorylation of CHEK1.By similarity

    Protein-protein interaction databases

    BioGridi675270. 1 interaction.
    STRINGi9031.ENSGALP00000040314.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8AYC9.
    SMRiQ8AYC9. Positions 3-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 265257Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 265265Interaction with CLSPNBy similarityAdd
    BLAST
    Regioni391 – 47686Autoinhibitory regionBy similarityAdd
    BLAST

    Domaini

    The autoinhibitory region (AIR) inhibits the activity of the kinase domain.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000216658.
    HOVERGENiHBG002590.
    InParanoidiQ8AYC9.
    KOiK02216.
    PhylomeDBiQ8AYC9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8AYC9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPFVEDWD LVQTLGEGAY GEVQLAVNRR TEEAVAVKIV DMKRAADCPE    50
    NIKKEICINK MLNHENVVKF YGHRREGATQ YLFLEYCSGG ELFDRIEPDI 100
    GMPEPEAQRF FQQLIAGVVY LHSMGITHRD LKPENLLLDE RDNLKISDFG 150
    LATVFKHNGR ERLLNKMCGT LPYVAPELLR RPEFRAEPVD VWACGVVLTA 200
    MLAGELPWDQ PSDSCQEYSD WKERKTYLAP WRKIDSAPLA LLHKILTENP 250
    TARITIPDIK KDRWYCRPLK KGTKRGRVSS GGVTESPGAL PKHIRSDTDF 300
    SPVKSALGED KASYSTSQPE PGTGGALWDS STGSIDRLVQ GISFSQPACP 350
    EHMLLNSQLL GTPGSSQSPW QRLVRRMTRF FTKLDADGSY RSLRDVCEKM 400
    GYGWKQSCTN QVTISTTDRR NNKLIFKVNL LEMESRILVD FRLSKGDGLE 450
    FKRHFLKIKG KLSDVVSTQK VWLPPP 476
    Length:476
    Mass (Da):53,849
    Last modified:March 1, 2003 - v1
    Checksum:i0E54EE2196EF0C15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525027 mRNA. Translation: AAN33019.1.
    RefSeqiNP_989676.1. NM_204345.1.
    UniGeneiGga.4288.
    Gga.48286.

    Genome annotation databases

    GeneIDi374260.
    KEGGigga:374260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525027 mRNA. Translation: AAN33019.1 .
    RefSeqi NP_989676.1. NM_204345.1.
    UniGenei Gga.4288.
    Gga.48286.

    3D structure databases

    ProteinModelPortali Q8AYC9.
    SMRi Q8AYC9. Positions 3-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 675270. 1 interaction.
    STRINGi 9031.ENSGALP00000040314.

    Proteomic databases

    PaxDbi Q8AYC9.
    PRIDEi Q8AYC9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 374260.
    KEGGi gga:374260.

    Organism-specific databases

    CTDi 3703.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000216658.
    HOVERGENi HBG002590.
    InParanoidi Q8AYC9.
    KOi K02216.
    PhylomeDBi Q8AYC9.

    Enzyme and pathway databases

    Reactomei REACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
    REACT_115715. ATM mediated phosphorylation of repair proteins.
    REACT_115915. Chk1-controlled and DNA-damage induced centrosome duplication.

    Miscellaneous databases

    NextBioi 20813751.
    PROi Q8AYC9.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chk1-deficient tumour cells are viable but exhibit multiple checkpoint and survival defects."
      Zachos G., Rainey M.D., Gillespie D.A.F.
      EMBO J. 22:713-723(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION AT SER-345.

    Entry informationi

    Entry nameiCHK1_CHICK
    AccessioniPrimary (citable) accession number: Q8AYC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3