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Q8AY29

- CEGTA_XENLA

UniProt

Q8AY29 - CEGTA_XENLA

Protein

Ceramide glucosyltransferase-A

Gene

ugcg-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. Glycosphingolipids are required for convergence extension movements during early development.1 Publication

    Catalytic activityi

    UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. ceramide glucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. glycosphingolipid catabolic process Source: UniProtKB
    2. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Glycosyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00222.

    Protein family/group databases

    CAZyiGT21. Glycosyltransferase Family 21.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ceramide glucosyltransferase-A (EC:2.4.1.80)
    Short name:
    XLCGT1 Publication
    Alternative name(s):
    UDP-glucose ceramide glucosyltransferaseImported
    Gene namesi
    Name:ugcg-a
    Synonyms:ugcgImported
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6254211. ugcg.

    Subcellular locationi

    Golgi apparatus membrane Sequence Analysis; Multi-pass membrane protein 1 PublicationSequence Analysis

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 394394Ceramide glucosyltransferase-APRO_0000376854Add
    BLAST

    Expressioni

    Tissue specificityi

    At the late gastrula stage, weakly expressed ubiquitously. As neurulation proceeds (stages 15-16), expression moves towards the dorsal structures: involuted paraxial mesoderm and neural folds. In the tailbud embryo (stage 28), expression is restricted to the notochord. At later stages (stage 35), expression remains in the notochord and also appears weakly in the cephalic region.1 Publication

    Developmental stagei

    Expressed both maternally and zygotically. Expressed at all stages of oogenesis except in previtellogenic oocytes. During embryogenesis, weakly expressed as early as the one-cell stage shortly after fertilization, and remains at a relatively uniform level throughout embryogenesis, persisting at least until stage 35 (early tadpole).1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8AY29.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010LumenalSequence Analysis
    Topological domaini33 – 195163CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini216 – 28772LumenalSequence AnalysisAdd
    BLAST
    Topological domaini305 – 3095CytoplasmicSequence Analysis
    Topological domaini329 – 34820LumenalSequence AnalysisAdd
    BLAST
    Topological domaini370 – 39425CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei288 – 30417HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 32819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Sequence Analysis

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK00720.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR025993. Ceramide_glucosylTrfase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF13506. Glyco_transf_21. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8AY29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVLDLALQG LAIFGCVLFF VLWFMHFLSI VYTRLHLNKK ISDKQPYSKL    50
    PGVSLLKPLK GVDPNLINNL ETFFELDYPK FEILLCVQDL DDPAVDVCKK 100
    LLGKYPSDDA KLFIGGKKVG INPKINNLMP GYEVAKYDLI WICDSGIKVK 150
    PDTLTDMANQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRSYISA 200
    NVTGFKCVTG MSCLMRKEVL DQAGGLIAFA QYIAEDYFMA KAIADRGWKF 250
    SMATQVAMQN SGCYSISQFQ SRMIRWAKLR INMLPATIIC EPISECFVAS 300
    LIIGWAAHHI FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG PLNFSKLDYA 350
    VAWFIRESMT IYIFLSALWD PTISWRTGRF RLRCGGTAEE ILDV 394
    Length:394
    Mass (Da):44,651
    Last modified:March 1, 2003 - v1
    Checksum:iD25C6236C6A3388A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081D → V in AAH89245. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY112732 mRNA. Translation: AAM49061.1.
    BC089245 mRNA. Translation: AAH89245.1.
    RefSeqiNP_001083944.1. NM_001090475.1.
    UniGeneiXl.21362.

    Genome annotation databases

    GeneIDi399204.
    KEGGixla:399204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY112732 mRNA. Translation: AAM49061.1 .
    BC089245 mRNA. Translation: AAH89245.1 .
    RefSeqi NP_001083944.1. NM_001090475.1.
    UniGenei Xl.21362.

    3D structure databases

    ProteinModelPortali Q8AY29.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT21. Glycosyltransferase Family 21.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399204.
    KEGGi xla:399204.

    Organism-specific databases

    CTDi 399204.
    Xenbasei XB-GENE-6254211. ugcg.

    Phylogenomic databases

    KOi K00720.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR025993. Ceramide_glucosylTrfase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF13506. Glyco_transf_21. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis."
      Luque M.E., Crespo P.M., Monaco M.E., Aybar M.J., Daniotti J.L., Sanchez S.S.
      Dev. Dyn. 237:112-123(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: EggImported.

    Entry informationi

    Entry nameiCEGTA_XENLA
    AccessioniPrimary (citable) accession number: Q8AY29
    Secondary accession number(s): Q5FWQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3