Reviewed, UniProtKB/Swiss-Prot Q8AXY5 (C356_FUNHE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 3A56
    EC=1.14.14.1
Alternative name(s):
    CYPIIIA56

Gene names

Name:

cyp3a56

Organism

Fundulus heteroclitus (Killifish) (Mummichog)

Taxonomic identifier

8078 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Cyprinodontiformes › Fundulidae › Fundulus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Putative steroid 6-beta-hydroxylase.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. UniProtKB Q9PVE8
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Potential. Microsome membrane; Peripheral membrane protein Potential.
Tissue specificity	Highly expressed in liver and intestine. Moderate expression in gill and spleen. Low expression in kidney, brain and heart. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.
Caution	Due to a recent gene duplication event, CYP3A30 and CYP3A56 are very similar. Because of this it was not possible to distinguish between the two genes when measuring the tissue expression. Ref.1

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Cytochrome P450 3A56		PRO_0000051810
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity UniProtKB P04798

Sequences

	Sequence		Length	Mass (Da)
	Q8AXY5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BAF945B260CA0220	FASTA	496	57,054
	10 20 30 40 50 60 MGYFYLTAET WTLLVAFVTL LLIYAYWPYG TFKRLGISGP KPVPFFGTML HYRRGFFTFD 70 80 90 100 110 120 EECKKKYGKV WGIYDGRQPV LCVTDPEIIK AVLVKECLSF FTNRRNFHLN GPLYDALSVA 130 140 150 160 170 180 EDDQWKRIRS VLSPSFTSGR LKEMFEIMKN HSANLIRSMK KKADKDEPLD LKEFFGSYSM 190 200 210 220 230 240 DVVTSTAFSV DIDSLNNPSD PFVTNIKKML KFDFLNPLFL AVAFFPFLGP ILEKFELSFF 250 260 270 280 290 300 PKSVTDFFYA SLEKIKSNRE ASQQKSRVDF LQLMIDSQKN SGAQQDKSLT DHEILSQSMI 310 320 330 340 350 360 FIFAGYETSS SSLTFLAYNL ATNPEVMKKL QEEIDATFPN KAPVHYQPLM EMEYLDCVIN 370 380 390 400 410 420 ESLRLFPIAA RLERVAKAAV EINGIVIPKD MVVMIPTWPL HRDPEIWPEP EAFKPERFSK 430 440 450 460 470 480 KNKDNIDPYI YMPFGSGPRN CIGMRFALVL IKLAVVEILQ QYSFSVCKET EVPFEMDIQG 490 LLAPKRPIQL KLVPRS

References

[1]	"Hepatic versus extrahepatic expression of CYP3A30 and CYP3A56 in adult killifish (Fundulus heteroclitus)." Hegelund T., Celander M.C. Aquat. Toxicol. 64:277-291(2003) [PubMed: 12842592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Isolation of a cytochrome P450 3A cDNA sequence (CYP3A30) from the marine teleost Fundulus heteroclitus and phylogenetic analyses of CYP3A genes." Celander M.C., Stegeman J.J. Biochem. Biophys. Res. Commun. 236:306-312(1997) [PubMed: 9240431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 312-435. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY143428 mRNA. Translation: AAN38837.1.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8AXY5.
Enzyme and pathway databases
BRENDA	1.14.14.1. 39477.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C356_FUNHE

Accession

Primary (citable) accession number: Q8AXY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)