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Protein

Acidic phospholipase A2 BthA-1

Gene
N/A
Organism
Bothrops jararacussu (Jararacussu)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that induces edema (activity that is inhibited by EDTA and dexamethasone), inhibits phospholipid-dependent collagen/ADP-induced platelet aggregation, possess hypotensive as well as anticoagulant activities. In addition, this enzyme shows bactericidal activity against E.coli and S.aureus. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.2 Publications

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.2 Publications

Enzyme regulationi

Inhibited by EDTA and BPB.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43CalciumCombined sources2 Publications1
Metal bindingi47Calcium; via amide nitrogenCombined sources2 Publications1
Metal bindingi48Calcium; via amide nitrogenCombined sources2 Publications1
Active sitei631 Publication1
Metal bindingi64CalciumCombined sources2 Publications1
Active sitei1051 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Hypotensive agent, Platelet aggregation inhibiting toxin, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic phospholipase A2 BthA-1 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
BOJU-III
BthA-I-PLA2
Hypotensive phospholipase A2
Phosphatidylcholine 2-acylhydrolase
OrganismiBothrops jararacussu (Jararacussu)
Taxonomic identifieri8726 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 show no toxicity when injected into mice: 25 mg/kg by intraperitoneal injection, 7.5 mg/kg by intravenous injection, and 0.5 mg/kg by intracerebroventricular injection.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_000041380417 – 138Acidic phospholipase A2 BthA-1Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 131Combined sources3 Publications
Disulfide bondi44 ↔ 60Combined sources3 Publications
Disulfide bondi59 ↔ 111Combined sources3 Publications
Disulfide bondi65 ↔ 138Combined sources3 Publications
Disulfide bondi66 ↔ 104Combined sources3 Publications
Disulfide bondi73 ↔ 97Combined sources3 Publications
Disulfide bondi91 ↔ 102Combined sources3 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.2 Publications

Interactioni

Subunit structurei

Homodimer; non-covalently linked.3 Publications

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 29Combined sources12
Helixi33 – 38Combined sources6
Turni41 – 43Combined sources3
Beta strandi44 – 47Combined sources4
Helixi55 – 68Combined sources14
Turni75 – 77Combined sources3
Beta strandi82 – 85Combined sources4
Beta strandi88 – 91Combined sources4
Helixi96 – 114Combined sources19
Helixi115 – 118Combined sources4
Helixi121 – 123Combined sources3
Helixi128 – 130Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U73X-ray1.90A/B17-138[»]
1UMVX-ray1.79X17-138[»]
1Z76X-ray1.85A/B17-138[»]
1ZL7X-ray1.60A17-138[»]
1ZLBX-ray0.97A17-138[»]
ProteinModelPortaliQ8AXY1.
SMRiQ8AXY1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8AXY1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8AXY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLWIMAVL LVGVEGSLWQ FGKMINYVMG ESGVLQYLSY GCYCGLGGQG
60 70 80 90 100
QPTDATDRCC FVHDCCYGKV TGCDPKIDSY TYSKKNGDVV CGGDDPCKKQ
110 120 130
ICECDRVATT CFRDNKDTYD IKYWFYGAKN CQEKSEPC
Length:138
Mass (Da):15,456
Last modified:March 1, 2003 - v1
Checksum:i513647907BFD0F4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145836 mRNA. Translation: AAN37410.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145836 mRNA. Translation: AAN37410.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U73X-ray1.90A/B17-138[»]
1UMVX-ray1.79X17-138[»]
1Z76X-ray1.85A/B17-138[»]
1ZL7X-ray1.60A17-138[»]
1ZLBX-ray0.97A17-138[»]
ProteinModelPortaliQ8AXY1.
SMRiQ8AXY1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Miscellaneous databases

EvolutionaryTraceiQ8AXY1.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2A_BOTJR
AccessioniPrimary (citable) accession number: Q8AXY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This enzyme has been found to be not myotoxic, not cytotoxic, not hemorrhagic and not lethal.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.