Reviewed, UniProtKB/Swiss-Prot Q8AXL1 (SAT1_CHICK)

Names and origin

Protein names

Recommended name:
    Diamine acetyltransferase 1
    EC=2.3.1.57
Alternative name(s):
    Spermidine/spermine N(1)-acetyltransferase 1
      Short name=SSAT-1
      Short name=SSAT
    Putrescine acetyltransferase
    Polyamine N-acetyltransferase 1

Gene names

Name:	SAT1
Synonyms:	SAT, SSAT

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Preferentially expressed in the dorsal-temporal quadrant of the developing retina. There was a sharp increase in retinal SSAT levels during the transition stage from proliferation (E7) to early differentiation (E10). SSAT was found in Muller glial cells and its distribution pattern in these cells closely followed the three differentiation axis of the developing retina, with a central-dorsal-temporal preference. Ref.1
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Diamine acetyltransferase 1		PRO_0000074596
Regions
	Domain	4 – 171	168	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	126 – 128	3	Substrate binding By similarity
	Region	133 – 136	4	Acetyl-CoA binding By similarity
	Region	140 – 143	4	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8AXL1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 884F62EE071A368F	FASTA	171	19,965
	10 20 30 40 50 60 MASFSIRAAR PEDCSDLLRL IKELAKYEDM EDQVVLTEKE LLEDGFGEHP FYHCLVAEVP 70 80 90 100 110 120 KEQWSSEGHS IVGFAMYYFT YDPWIGKLLY LEDFYVMAEY RGLGIGSEIL KNLSQVAVKC 130 140 150 160 170 RCSSMHFLVA EWNEPSIRFY KRRGASDLST EEGWRLFKID KEYLLKMATE E

References

[1]	"Expression of spermidine/spermine N(1)-acetyltransferase in the Muller glial cells of the developing chick retina." Witte R.L., Godbout R. Exp. Eye Res. 74:605-613(2002) [PubMed: 12076082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Retina.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF402003 mRNA. Translation: AAN76653.1.
IPI	IPI00585463.
RefSeq	NP_989517.1.
UniGene	Gga.9589
3D structure databases
SMR	Q8AXL1. Positions 2-171.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8AXL1.
Genome annotation databases
Ensembl	ENSGALT00000026368; ENSGALP00000026318; ENSGALG00000016348; Gallus gallus. [Genome view] ENSGALT00000036635; ENSGALP00000035846; ENSGALG00000016348; Gallus gallus. [Genome view]
GeneID	374006.
KEGG	gga:374006.
Organism-specific databases
CTD	374006.
Phylogenomic databases
eggNOG	veNOG15683.
HOGENOM	HBG742014.
HOVERGEN	Q8AXL1.
InParanoid	Q8AXL1.
Enzyme and pathway databases
BRENDA	2.3.1.57. 4.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT1_CHICK

Accession

Primary (citable) accession number: Q8AXL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)