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Q8AXC6 (KIT_TAKRU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mast/stem cell growth factor receptor Kit
Short name=SCFR
EC=2.7.10.1
Alternative name(s):
Tyrosine-protein kinase kit
Gene names
Name:kit
OrganismTakifugu rubripes (Japanese pufferfish) (Fugu rubripes) [Reference proteome]
Taxonomic identifier31033 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Tetraodontiformes › Tetradontoidea › Tetraodontidae › Takifugu

Protein attributes

Sequence length984 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine kitlg/scf and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Post-translational modification

Ubiquitinated By similarity. Rapidly ubiquitinated after autophosphorylation induced by kitlg/scf binding, leading to internalization and degradation By similarity.

Autophosphorylated on tyrosine residues. Phosphorylated tyrosine residues are important for interaction with specific binding partners By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 984963Mast/stem cell growth factor receptor Kit
PRO_0000248881

Regions

Topological domain22 – 514493Extracellular Potential
Transmembrane515 – 53521Helical; Potential
Topological domain536 – 984449Cytoplasmic Potential
Domain23 – 9775Ig-like C2-type 1
Domain98 – 197100Ig-like C2-type 2
Domain203 – 30098Ig-like C2-type 3
Domain311 – 39585Ig-like C2-type 4
Domain398 – 498101Ig-like C2-type 5
Domain579 – 926348Protein kinase
Nucleotide binding586 – 5938ATP By similarity
Nucleotide binding661 – 6677ATP By similarity

Sites

Active site7811Proton acceptor By similarity
Metal binding5581Magnesium By similarity
Metal binding7861Magnesium By similarity
Metal binding7991Magnesium By similarity
Binding site6131ATP By similarity
Binding site7851ATP By similarity

Amino acid modifications

Modified residue5581Phosphotyrosine; by autocatalysis By similarity
Modified residue5601Phosphotyrosine; by autocatalysis By similarity
Modified residue6901Phosphotyrosine; by autocatalysis By similarity
Modified residue7071Phosphotyrosine; by autocatalysis By similarity
Modified residue8121Phosphotyrosine; by autocatalysis By similarity
Modified residue9251Phosphotyrosine; by autocatalysis By similarity
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation2601N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Glycosylation3951N-linked (GlcNAc...) Potential
Glycosylation4481N-linked (GlcNAc...) Potential
Glycosylation4761N-linked (GlcNAc...) Potential
Disulfide bond44 ↔ 87 By similarity
Disulfide bond129 ↔ 178 By similarity
Disulfide bond144 ↔ 175 By similarity
Disulfide bond226 ↔ 284 By similarity
Disulfide bond421 ↔ 487 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8AXC6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 27D1EDC837CE8295

FASTA984110,732
        10         20         30         40         50         60 
MEYHWILLCV SLCFTFHPGD TKPTITPAGT YVVVSLNAPL ELQCQGEKAM QWQREERPKV 

        70         80         90        100        110        120 
RGETKVDGKS TLYIPKAHPA HMGRYICLEE TSQERASIYI YVKDPDNPFR KSMVSNILSR 

       130        140        150        160        170        180 
EGDSASIPCL ATDPSLENLQ LKTCSSKALA SGLHFSPSLE QGIIIHNTQK SYEGCYVCTG 

       190        200        210        220        230        240 
RLKETNVRSH DYHLTVRPVP VAPPVIMMQA PKRVILIRDE SLYLTCNTTN VNGNIKLKWV 

       250        260        270        280        290        300 
APLGSQPAKV DGSSRILTEN FTQARSATLH IAAVRIQDTG RYQCEAENEK GVSTQSVWLD 

       310        320        330        340        350        360 
VFEKGFMYSN PVNNGTIQVR AGESLLLSVS IEAYPMPRSA SWSFMGRGLH NTSDHVITTR 

       370        380        390        400        410        420 
SHEYTYSSEL KLVRLKMSEG GVYTFQASNG DASVNHTFTI FVISKPEIVS HEGPVDGQVR 

       430        440        450        460        470        480 
CVAEGFPAPQ ITWYYCEQPY ARCSQQVNAT QEEQNVITVT LFSPLFGKTE VESRVNISRG 

       490        500        510        520        530        540 
RFSTLECVAT VEGEQAFTLF SISERTISHD LFSPLLIGSV SAACILCLIL IVLFYKYMQK 

       550        560        570        580        590        600 
PKYQIQWKVI EGIHGNNYVY IDPTQLPYDH QWEFPRKNLR FGKTLGSGAF GKVVEATAYG 

       610        620        630        640        650        660 
LANEDSMMTV AVKMLKSSAH STEKEALMSE LKVLIYLGNH INIVNLLGAC TVGGPTLVIT 

       670        680        690        700        710        720 
EYCCFGDLLN FLRRKRESFI CFKLEEDCHY RNIMLQREMA GDSLNGYMTM RPSAAGKPSS 

       730        740        750        760        770        780 
SSSSEKRRSL REGSPYVEED SESEMFDEDS LSLDTEDLLS FSYQVAKGME FLTSKNCIHR 

       790        800        810        820        830        840 
DLAARNILLT QGRVAKICDF GLARDINTDS NYVVKGNARL PVKWMSPESI FECVYTFESD 

       850        860        870        880        890        900 
VWSYGILLWE IFSLGNSPYP GMPVDAKFYK LIKEGYRMDA PEFAPSEMYQ IMRSCWDADP 

       910        920        930        940        950        960 
LNRPPFRKVV ERIEQQLSDT TKHIYLNFSS RVPVMPRGRE ESSTHSMASQ PFNSAGNNSP 

       970        980 
PSRPLLLHHE VFLEGTEPFR VQRV

« Hide

References

[1]"Characterization of the platelet-derived growth factor receptor alpha and c-kit genes in the pufferfish Fugu rubripes."
Williams H., Brenner S., Venkatesh B.
DNA Seq. 13:263-270(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF456419 Genomic DNA. Translation: AAN87555.1.

3D structure databases

HSSPHSSP built from PDB template 1PKG based on UniProtKB P10721.
ProteinModelPortalQ8AXC6.
SMRQ8AXC6. Positions 537-921.
ModBaseSearch...

Protein-protein interaction databases

STRING31033.ENSTRUP00000038813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0515.
InParanoidQ8AXC6.
OrthoDBEOG4W0XCD.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamPF07679. I-set. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTSM00409. IG. 3 hits.
SM00408. IGc2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS50835. IG_LIKE. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKIT_TAKRU
AccessionPrimary (citable) accession number: Q8AXC6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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