Q8AW93 (DTX1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase DTX1
EC=6.3.2.-

Alternative name(s):
Protein deltex-1
Short name=xDtx1

Gene names

Name:

dtx1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	May form a homo- or heterodimer with other members of the Deltex family. Probably interacts with Notch1 By similarity.
Tissue specificity	Specifically expressed in regions undergoing neuronal differentiation. Mainly colocalizes with Notch1.
Developmental stage	In the tailbud stage, it is expressed in the olfactory bulbs, pineal complex and along the neural tube according to an antero-posterior gradient showing a gap at the midbrain-hindbrain boundary. At tadpole stage, it is expressed in the differentiating retina, in the neuronal fibers of the outer and inner plexiform layers, while its expression in the pineal complex becomes restricted to the photosensitive frontal organ. Ref.1
Domain	The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.
Sequence similarities	Belongs to the Deltex family. Contains 1 RING-type zinc finger. Contains 2 WWE domains.

Ontologies

Keywords
Biological process	Notch signaling pathway Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	E3 ubiquitin-protein ligase DTX1		PRO_0000219082
Regions
	Domain	13 – 93	81	WWE 1
	Domain	94 – 170	77	WWE 2
	Zinc finger	413 – 474	62	RING-type
	Compositional bias	226 – 243	18	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q8AW93 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0BBE3411882F8F36	FASTA	623	68,333
	10 20 30 40 50 60 MSRPGVLLPV NGHNFGSQGG PPRVVVWEWL NEHGRWRPYT ATVCHHIENA LREDGRGRVA 70 80 90 100 110 120 LGQVDAQLTP YVIDLQTMHQ YRQDTGTIRP VRRNFFEPSS APGKGIVWEW ENDAGSWTPY 130 140 150 160 170 180 DTEICIAIQN AYEKHHPYLD LTTLGFCYLV HFHSMCQVNR QTHRKRRLRR RMDLAYPLTM 190 200 210 220 230 240 GSIPKSQSWP VGSGSGLPCS CPQCLLVNST RAASNAILAS QRIKVPSGPP PALPPPPPPP 250 260 270 280 290 300 IHPSGLRQSN TYSGGAAGWG RTGEGMRSTG GIRNGSGFSR SQSVPGAAPY PGQNNLNRPG 310 320 330 340 350 360 EQRTSGSSSR ASIPPGVPAL PVKNLNGSGP VHPALAGMTG ILMCAAGLPV CLTRAPKPIL 370 380 390 400 410 420 HPPPVSKEDI KPVSGVSGIC RKTKKKHLKK SKNPEEVVRR YIQKVKSPPD EDCTICMERL 430 440 450 460 470 480 VTASGYDGVL SHRGIRAELV GKLGKCNHMY HVLCPVAMYN NGNKDGSLQC PTCKAIYGEK 490 500 510 520 530 540 TGTQPPGKME FHVIPHSLPG FSDCKTIRIV YDIPSGMQGP EHPNPGKKFT ARGFPRHCYL 550 560 570 580 590 600 PDNDKGRKVL RLLLAAWERR LIFAIGTSST TGESNTVVWN EIHHKTEFGS NLTGHGYPDP 610 620 NYLDNVLTEL HRQGVMEERS LPY

References

[1]	"Xdtx1, a Xenopus Deltex homologue expressed in differentiating neurons and in photoreceptive organs." Andreazzoli M., Marracci S., Panattoni M., Nardi I. Gene Expr. Patterns 2:283-287(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ431211 mRNA. Translation: CAD26517.1.
RefSeq	NP_001082450.1. NM_001088981.1.
UniGene	Xl.26491.
3D structure databases
ProteinModelPortal	Q8AW93.
SMR	Q8AW93. Positions 391-497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	398477.
KEGG	xla:398477.
Organism-specific databases
CTD	1840.
Xenbase	XB-GENE-865444. dtx1.
Phylogenomic databases
HOVERGEN	HBG007213.
KO	K06058.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR004170. WWE-dom. IPR018123. WWE-dom_subgr. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF02825. WWE. 2 hits. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00678. WWE. 2 hits. [Graphical view]
PROSITE	PS50918. WWE. 2 hits. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DTX1_XENLA

Accession

Primary (citable) accession number: Q8AW93

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program