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Protein

E3 ubiquitin-protein ligase DTX1

Gene

dtx1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri413 – 474RING-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processNotch signaling pathway, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DTX1 (EC:2.3.2.27By similarity)
Alternative name(s):
Protein deltex-1
Short name:
xDtx1
RING-type E3 ubiquitin transferase DTX1Curated
Gene namesi
Name:dtx1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865444. dtx1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190821 – 623E3 ubiquitin-protein ligase DTX1Add BLAST623

Expressioni

Tissue specificityi

Specifically expressed in regions undergoing neuronal differentiation. Mainly colocalizes with Notch1.

Developmental stagei

In the tailbud stage, it is expressed in the olfactory bulbs, pineal complex and along the neural tube according to an antero-posterior gradient showing a gap at the midbrain-hindbrain boundary. At tadpole stage, it is expressed in the differentiating retina, in the neuronal fibers of the outer and inner plexiform layers, while its expression in the pineal complex becomes restricted to the photosensitive frontal organ.1 Publication

Interactioni

Subunit structurei

May form a homo- or heterodimer with other members of the Deltex family. Probably interacts with Notch1.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8AW93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 93WWE 1PROSITE-ProRule annotationAdd BLAST81
Domaini94 – 170WWE 2PROSITE-ProRule annotationAdd BLAST77

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi226 – 243Pro-richAdd BLAST18

Domaini

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.By similarity

Sequence similaritiesi

Belongs to the Deltex family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri413 – 474RING-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG007213.
KOiK06058.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF02825. WWE. 2 hits.
SMARTiView protein in SMART
SM00678. WWE. 2 hits.
PROSITEiView protein in PROSITE
PS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8AW93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPGVLLPV NGHNFGSQGG PPRVVVWEWL NEHGRWRPYT ATVCHHIENA
60 70 80 90 100
LREDGRGRVA LGQVDAQLTP YVIDLQTMHQ YRQDTGTIRP VRRNFFEPSS
110 120 130 140 150
APGKGIVWEW ENDAGSWTPY DTEICIAIQN AYEKHHPYLD LTTLGFCYLV
160 170 180 190 200
HFHSMCQVNR QTHRKRRLRR RMDLAYPLTM GSIPKSQSWP VGSGSGLPCS
210 220 230 240 250
CPQCLLVNST RAASNAILAS QRIKVPSGPP PALPPPPPPP IHPSGLRQSN
260 270 280 290 300
TYSGGAAGWG RTGEGMRSTG GIRNGSGFSR SQSVPGAAPY PGQNNLNRPG
310 320 330 340 350
EQRTSGSSSR ASIPPGVPAL PVKNLNGSGP VHPALAGMTG ILMCAAGLPV
360 370 380 390 400
CLTRAPKPIL HPPPVSKEDI KPVSGVSGIC RKTKKKHLKK SKNPEEVVRR
410 420 430 440 450
YIQKVKSPPD EDCTICMERL VTASGYDGVL SHRGIRAELV GKLGKCNHMY
460 470 480 490 500
HVLCPVAMYN NGNKDGSLQC PTCKAIYGEK TGTQPPGKME FHVIPHSLPG
510 520 530 540 550
FSDCKTIRIV YDIPSGMQGP EHPNPGKKFT ARGFPRHCYL PDNDKGRKVL
560 570 580 590 600
RLLLAAWERR LIFAIGTSST TGESNTVVWN EIHHKTEFGS NLTGHGYPDP
610 620
NYLDNVLTEL HRQGVMEERS LPY
Length:623
Mass (Da):68,333
Last modified:March 1, 2003 - v1
Checksum:i0BBE3411882F8F36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ431211 mRNA. Translation: CAD26517.1.
RefSeqiNP_001082450.1. NM_001088981.1.
UniGeneiXl.26491.

Genome annotation databases

GeneIDi398477.
KEGGixla:398477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ431211 mRNA. Translation: CAD26517.1.
RefSeqiNP_001082450.1. NM_001088981.1.
UniGeneiXl.26491.

3D structure databases

ProteinModelPortaliQ8AW93.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398477.
KEGGixla:398477.

Organism-specific databases

CTDi398477.
XenbaseiXB-GENE-865444. dtx1.

Phylogenomic databases

HOVERGENiHBG007213.
KOiK06058.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF02825. WWE. 2 hits.
SMARTiView protein in SMART
SM00678. WWE. 2 hits.
PROSITEiView protein in PROSITE
PS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDTX1_XENLA
AccessioniPrimary (citable) accession number: Q8AW93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 1, 2003
Last modified: February 15, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.