Q8AVY8 (DH12B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Estradiol 17-beta-dehydrogenase 12-B
EC=1.1.1.62

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 12-B
Short name=17-beta-HSD 12-B

Gene names

Name:

hsd17b12-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Estradiol 17-beta-dehydrogenase 12-B		PRO_0000248374
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	187 – 207	21	Helical; Potential
	Transmembrane	281 – 301	21	Helical; Potential
	Nucleotide binding	54 – 83	30	NADP By similarity
Sites
	Active site	207	1	Proton acceptor By similarity
	Binding site	194	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8AVY8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A5D8971F8AE72AD0	FASTA	318	34,861
	10 20 30 40 50 60 MAPESLAEVP GCNCFWYLGV VAATWWGLRA AWCLLNGARV WVLGSGAQVG PTIGKWAVVT 70 80 90 100 110 120 GATDGIGKAY AEELARRGMN IVLISRSPEK LEEAAIHIKQ KFKVETKIIA ADFGKPTEIY 130 140 150 160 170 180 ERIEAGLRDL EIGVLVNNVG ISYEYPEYFL EIPDLENTLD KMININIMSV CQMTRLVLPG 190 200 210 220 230 240 MLGRGKGVVL NISSASGMYP VPLLTVYSAT KAFVDFFSRG LHAEYRSKGV TVQSVLPFFV 250 260 270 280 290 300 ATKLAKIRKP TWDKPSPETY VRSALNTVGL QTQTNGYLPH AITGWISTSL VPVSAAISMG 310 MKMNKGLRAR FLKKAKQN

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC041194 mRNA. Translation: AAH41194.1.
RefSeq	NP_001080055.1. NM_001086586.1.
UniGene	Xl.21849.
3D structure databases
ProteinModelPortal	Q8AVY8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	379747.
KEGG	xla:379747.
Organism-specific databases
CTD	379747.
Xenbase	XB-GENE-945234. hsd17b12.
Phylogenomic databases
HOVERGEN	HBG005478.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00044. K10251.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DH12B_XENLA

Accession

Primary (citable) accession number: Q8AVY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2003
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program