Q8AVU2 (UB2SB_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S-B
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S-B
Ubiquitin-protein ligase S-B

Gene names

Name:	ube2s-b
Synonyms:	ube2s.1-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB free ubiquitin chain polymerization Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Ubiquitin-conjugating enzyme E2 S-B		PRO_0000390432
Sites
	Active site	95	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8AVU2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EA930D5B61E9D251	FASTA	211	23,359
	10 20 30 40 50 60 MNSNVENLPP HIIRRVYKEV STLTSDPPEG IKIIPNEEDI TDVQVNIEGP EGTPYAGGMF 70 80 90 100 110 120 RMKLILGKDF PAAPPKGYFL TKIFHPNVSN NGEICVNVLK KDWKAELGIR HVLLTIKCLL 130 140 150 160 170 180 IHPNPESALN EEAGRLLLEN YEEYASRARL MTDIHAQGTS LRGKDPTDPC SSASTPVVSG 190 200 210 DGPMAKKHAG DRDKKLAAKK KTDKKRALRR L

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC041263 mRNA. Translation: AAH41263.1. BC106317 mRNA. Translation: AAI06318.1.
RefSeq	NP_001079388.1. NM_001085919.1.
UniGene	Xl.32557.
3D structure databases
HSSP	HSSP built from PDB template 1JBB based on UniProtKB P52490.
ProteinModelPortal	Q8AVU2.
SMR	Q8AVU2. Positions 6-156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	379075.
KEGG	xla:379075.
Organism-specific databases
CTD	379075.
Xenbase	XB-GENE-1008024. ube2s.
Phylogenomic databases
HOVERGEN	HBG063308.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2SB_XENLA

Accession

Primary (citable) accession number: Q8AVU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	March 1, 2003
Last modified:	March 6, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program