Q8AVN9 (ZN346_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 346

Alternative name(s):
Double stranded RNA-binding protein ZFa
Short name=DsRBP-ZFa
Short name=ZFa
Just another zinc finger protein
Short name=Protein jaz

Gene names

Name:	znf346
Synonyms:	jaz

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds preferentially to dsRNA, but also to RNA-DNA hybrids. Ref.1
Subcellular location	Nucleus. Cytoplasm. Note: Primarily nuclear. Ref.1
Domain	The zinc-finger domains are required for binding to dsRNA, and also for nuclear localization By similarity. UniProtKB Q9R0B7
Sequence similarities	Contains 7 matrin-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	double-stranded RNA binding Inferred from direct assay Ref.1. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 524

524

Zinc finger protein 346

PRO_0000348934

Regions

Zinc finger

34 – 64

31

Matrin-type 1

Zinc finger

92 – 126

35

Matrin-type 2

Zinc finger

162 – 192

31

Matrin-type 3

Zinc finger

226 – 260

35

Matrin-type 4

Zinc finger

286 – 316

31

Matrin-type 5

Zinc finger

343 – 373

31

Matrin-type 6

Zinc finger

400 – 430

31

Matrin-type 7

Sites

Metal binding

36

1

Zinc 1 Ref.3

Metal binding

39

1

Zinc 1 Ref.3

Metal binding

52

1

Zinc 1; via tele nitrogen Ref.3

Metal binding

58

1

Zinc 1; via pros nitrogen Ref.3

Metal binding

97

1

Zinc 2 Ref.3

Metal binding

100

1

Zinc 2 Ref.3

Metal binding

113

1

Zinc 2; via tele nitrogen Ref.3

Metal binding

119

1

Zinc 2; via pros nitrogen Ref.3

Experimental info

Sequence conflict

397

1

G → V in AAC60260. Ref.1

Secondary structure

1

.

.

.

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.

.

.

.

.

.

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.

.

.

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.

.

.

.

524

Helix Strand Turn

Details...

Helix

17 – 26

10

Turn

27 – 30

4

Beta strand

33 – 36

4

Turn

37 – 40

4

Helix

46 – 54

9

Helix

56 – 68

13

Turn

93 – 95

3

Turn

98 – 101

4

Helix

107 – 114

8

Helix

117 – 127

11

Sequences

	Sequence		Length	Mass (Da)
	Q8AVN9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E55736038AC13D5C	FASTA	524	55,546
	10 20 30 40 50 60 MADEFGNGDA LDLPVGKDAV NSLIRENSHI FSDTQCKVCS AVLISESQKL AHYQSRKHAN 70 80 90 100 110 120 KVRRYMAINQ GEDSVPAKKF KAAPAEISDG EDRSKCCPVC NMTFSSPVVA ESHYIGKTHI 130 140 150 160 170 180 KNLRLREQGG VKEGMVNQAK KTRTPTVATK SDNKMDHSDR AKFCKLCHST FNNPLMAEQH 190 200 210 220 230 240 YAGKKHKKQE TKTQIMTIYT SSGQTPAQAP IPLNLNSPMP GSGSAGKGFS CDKCNIVLNS 250 260 270 280 290 300 IEQYQAHVSG AKHKNQLMSM TPLSEEGHQA VVAPSAIASG SAGKGFSCDT CNIVLNSIEQ 310 320 330 340 350 360 YQAHISGAKH KNHLKSMTPL SEEGHTAAVA PSAFASGSAG KGFSCDTCNI VLNSIEQYQA 370 380 390 400 410 420 HISGAKHKNH LMSMTPLSEE GHTAAVAPSA FASGSAGKGF SCDTCNIVLN SIEQYQAHVS 430 440 450 460 470 480 GAKHKNQLMS MTPSSEEGLP SAVGPSAFAS PLSAGGALSS GGPSGRGFCP SGDLTPKGPS 490 500 510 520 SFGSLPPLGS LLPPLYPPAH SSQPYVHDDT MSPDGYNYFN EDFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Xenopus zinc finger protein that specifically binds dsRNA and RNA-DNA hybrids." Finerty P.J. Jr., Bass B.L. J. Mol. Biol. 271:195-208(1997) [PubMed: 9268652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Tissue: Head.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.
[3]	"Solution structure of the N-terminal zinc fingers of the Xenopus laevis double-stranded RNA-binding protein ZFa." Moeller H.M., Martinez-Yamout M.A., Dyson H.J., Wright P.E. J. Mol. Biol. 351:718-730(2005) [PubMed: 16051273] [Abstract] Cited for: STRUCTURE BY NMR OF 2-128 IN COMPLEX WITH ZINC IONS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF005083 mRNA. Translation: AAC60260.1.
BC041713 mRNA. Translation: AAH41713.1.

RefSeq

NP_001080778.1. NM_001087309.1.

UniGene

Xl.386.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZU1	NMR	-	A	2-128	[»]

ProteinModelPortal

Q8AVN9.

SMR

Q8AVN9. Positions 2-128.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

380471.

KEGG

xla:380471.

Organism-specific databases

CTD

23567.

Xenbase

XB-GENE-967548. znf346.

Phylogenomic databases

HOVERGEN

HBG061007.

Family and domain databases

InterPro

IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 7 hits.
SM00451. ZnF_U1. 7 hits.
[Graphical view]

PROSITE

PS50171. ZF_MATRIN. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ZN346_XENLA

Accession

Primary (citable) accession number: Q8AVN9
Secondary accession number(s): O42147

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2003
Last modified:	July 27, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program