Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Frizzled-7-B

Gene

fzd7-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • convergent extension involved in gastrulation Source: UniProtKB
  • dorsal/ventral axis specification Source: UniProtKB
  • mesoderm formation Source: UniProtKB
  • protein localization Source: UniProtKB
  • tissue morphogenesis Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, G-protein coupled receptor, Receptor, Transducer
Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-7-B
Short name:
Fz-7-B
Short name:
Xfz7-B
Gene namesi
Name:fzd7-b
Synonyms:fz7-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 230ExtracellularSequence analysisAdd BLAST212
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Topological domaini252 – 262CytoplasmicSequence analysisAdd BLAST11
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 311ExtracellularSequence analysisAdd BLAST28
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 353CytoplasmicSequence analysisAdd BLAST21
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 397ExtracellularSequence analysisAdd BLAST23
Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
Topological domaini419 – 444CytoplasmicSequence analysisAdd BLAST26
Transmembranei445 – 465HelicalSequence analysisAdd BLAST21
Topological domaini466 – 502ExtracellularSequence analysisAdd BLAST37
Transmembranei503 – 523HelicalSequence analysisAdd BLAST21
Topological domaini524 – 548CytoplasmicSequence analysisAdd BLAST25

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi526K → M: Reduced binding to dvl1. 1 Publication1
Mutagenesisi531W → G: Reduced binding to dvl1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000028294919 – 548Frizzled-7-BSequence analysisAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 97PROSITE-ProRule annotation
Disulfide bondi44 ↔ 90PROSITE-ProRule annotation
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi81 ↔ 118PROSITE-ProRule annotation
Disulfide bondi107 ↔ 147PROSITE-ProRule annotation
Disulfide bondi111 ↔ 135PROSITE-ProRule annotation
Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, localized to the cranial neural crest and heart field where expression is retained at later stages in addition to new areas of expression in the neural tube, pronephros and tailbud. At tailbud stage, expressed in the pronephric duct, and broad head expression becomes more restricted to the hindbrain. In tadpoles, strongly expressed in the eye and the pericardium and myocardium of the developing heart.1 Publication

Interactioni

Subunit structurei

Interacts with wnt11 and sdc4. The extracellular domain interacts with the extracellular domain of pcdh8/papc (By similarity). Interacts (via C-terminus) with dvl1 (via PDZ domain).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dvl1P511413EBI-3870271,EBI-1538407From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8AVJ9. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ8AVJ9.
SMRiQ8AVJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 150FZPROSITE-ProRule annotationAdd BLAST120

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi526 – 531Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members1 Publication6
Motifi546 – 548PDZ-bindingSequence analysis3

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.By similarity
The extracellular domain interacts with Wnt proteins and the intracellular C-terminus transmits the Wnt signal.

Sequence similaritiesi

Belongs to the G-protein coupled receptor Fz/Smo family.Sequence analysis

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG006977.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiView protein in InterPro
IPR015526. Frizzled/SFRP.
IPR000539. Frizzled/Smoothened_TM.
IPR020067. Frizzled_dom.
IPR036790. Frizzled_dom_sf.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamiView protein in Pfam
PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
PRINTSiPR00489. FRIZZLED.
SMARTiView protein in SMART
SM00063. FRI. 1 hit.
SM01330. Frizzled. 1 hit.
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiView protein in PROSITE
PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8AVJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAPVSLLFC LFLQLCPSAQ QYHGEKGISV PDHGFCQPIS IPLCTDIAYN
60 70 80 90 100
QTIMPNLLGH TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL
110 120 130 140 150
EQAIPPCRSL CERARQGCEA LMNKFGFQWP ERLRCENFPV HGAGEICVGQ
160 170 180 190 200
NTSDNSPSGP TARPTLNLPD SITFQPHPHR DFTCPRQLKV PPYLGYRFLG
210 220 230 240 250
EKDCGAPCEP GKANGLMYFK EEEVRFARLW VGIWAILCGI STLFTVLTYL
260 270 280 290 300
VDMRRFSYPE RPIIFLSGCY FMVAVAYTAG FLLEERAVCV ERFSEDSYRT
310 320 330 340 350
VAQGTKKEGC TILFMILYFF GMASSIWWVI LALTWFLSAG MKWGHEAIEA
360 370 380 390 400
NSQYFHLAAW AVPAVKTITI LAMGQVDGDV LSGVCYVGIN SVDSLRGFVL
410 420 430 440 450
APLFVYLFLG TSFLLAGFVS LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV
460 470 480 490 500
MYTVPATIVL ACYFYEQAFR DTWEKTWLVH TCKGYAVPCP NYNFAPMSPD
510 520 530 540
FTVFMIKYLM TMIVGITSSF WIWSGKTLQS WRRFYHRLGN GSKGETAV
Length:548
Mass (Da):61,906
Last modified:March 1, 2003 - v1
Checksum:i97B3408B3C0EA273
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168L → P in AAD44331 (PubMed:10446283).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159106 mRNA. Translation: AAD44331.1.
BC042228 mRNA. Translation: AAH42228.1.
UniGeneiXl.633.

Similar proteinsi

Entry informationi

Entry nameiFZD7B_XENLA
AccessioniPrimary (citable) accession number: Q8AVJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families