Frizzled-7-B precursor - Xenopus laevis (African clawed frog)

Contribute

Send feedback

Read comments (?) or add your own

Q8AVJ9 (FZD7B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Frizzled-7-B
Short name=Fz-7-B
Short name=Xfz7-B

Gene names

Name:	fzd7-b
Synonyms:	fz7-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation. Ref.3 Ref.4 Ref.5 UniProtKB Q9PUK8
Subunit structure	Interacts with wnt11 and sdc4. The extracellular domain interacts with the extracellular domain of pcdh8/papc By similarity. Interacts (via C-terminus) with dvl1 (via PDZ domain). Ref.5 UniProtKB Q9PUK8
Subcellular location	Membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, localized to the cranial neural crest and heart field where expression is retained at later stages in addition to new areas of expression in the neural tube, pronephros and tailbud. At tailbud stage, expressed in the pronephric duct, and broad head expression becomes more restricted to the hindbrain. In tadpoles, strongly expressed in the eye and the pericardium and myocardium of the developing heart. Ref.1
Domain	Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway. The FZ domain is involved in binding with Wnt ligands By similarity. UniProtKB Q9VVX3 The extracellular domain interacts with Wnt proteins and the intracellular C-terminus transmits the Wnt signal. UniProtKB Q9PUK8
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from mutant phenotype Ref.4. Source: UniProtKB convergent extension involved in gastrulation Inferred from mutant phenotype Ref.4. Source: UniProtKB dorsal/ventral axis specification Inferred from mutant phenotype Ref.4. Source: UniProtKB mesoderm formation Inferred from sequence or structural similarity. Source: UniProtKB protein localization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW Wnt-activated receptor activity Inferred from electronic annotation. Source: InterPro Wnt-protein binding Inferred from sequence or structural similarity. Source: UniProtKB syndecan binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dvl1	P51141	3	EBI-3870271,EBI-1538407	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Potential

Chain

19 – 548

530

Frizzled-7-B

PRO_0000282949

Regions

Topological domain

19 – 230

212

Extracellular Potential

Transmembrane

231 – 251

Helical; Potential

Topological domain

252 – 262

Cytoplasmic Potential

Transmembrane

263 – 283

Helical; Potential

Topological domain

284 – 311

Extracellular Potential

Transmembrane

312 – 332

Helical; Potential

Topological domain

333 – 353

Cytoplasmic Potential

Transmembrane

354 – 374

Helical; Potential

Topological domain

375 – 397

Extracellular Potential

Transmembrane

398 – 418

Helical; Potential

Topological domain

419 – 444

Cytoplasmic Potential

Transmembrane

445 – 465

Helical; Potential

Topological domain

466 – 502

Extracellular Potential

Transmembrane

503 – 523

Helical; Potential

Topological domain

524 – 548

Cytoplasmic Potential

Domain

31 – 150

120

Motif

526 – 531

Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members

Motif

546 – 548

PDZ-binding

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

151

N-linked (GlcNAc...) Potential

Disulfide bond

36 ↔ 97

By similarity

Disulfide bond

44 ↔ 90

By similarity

Disulfide bond

81 ↔ 118

By similarity

Disulfide bond

107 ↔ 147

By similarity

Disulfide bond

111 ↔ 135

By similarity

Experimental info

Mutagenesis

526

K → M: Reduced binding to dvl1. Ref.5

Mutagenesis

531

W → G: Reduced binding to dvl1. Ref.5

Sequence conflict

168

L → P in AAD44331. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8AVJ9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 97B3408B3C0EA273
Show »

FASTA

548

61,906

        10         20         30         40         50         60 
MLAPVSLLFC LFLQLCPSAQ QYHGEKGISV PDHGFCQPIS IPLCTDIAYN QTIMPNLLGH 

        70         80         90        100        110        120 
TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL EQAIPPCRSL CERARQGCEA 

       130        140        150        160        170        180 
LMNKFGFQWP ERLRCENFPV HGAGEICVGQ NTSDNSPSGP TARPTLNLPD SITFQPHPHR 

       190        200        210        220        230        240 
DFTCPRQLKV PPYLGYRFLG EKDCGAPCEP GKANGLMYFK EEEVRFARLW VGIWAILCGI 

       250        260        270        280        290        300 
STLFTVLTYL VDMRRFSYPE RPIIFLSGCY FMVAVAYTAG FLLEERAVCV ERFSEDSYRT 

       310        320        330        340        350        360 
VAQGTKKEGC TILFMILYFF GMASSIWWVI LALTWFLSAG MKWGHEAIEA NSQYFHLAAW 

       370        380        390        400        410        420 
AVPAVKTITI LAMGQVDGDV LSGVCYVGIN SVDSLRGFVL APLFVYLFLG TSFLLAGFVS 

       430        440        450        460        470        480 
LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV MYTVPATIVL ACYFYEQAFR DTWEKTWLVH 

       490        500        510        520        530        540 
TCKGYAVPCP NYNFAPMSPD FTVFMIKYLM TMIVGITSSF WIWSGKTLQS WRRFYHRLGN 


GSKGETAV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel Xenopus frizzled genes expressed in developing heart and brain." Wheeler G.N., Hoppler S. Mech. Dev. 86:203-207(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Inducible gene expression in transgenic Xenopus embryos." Wheeler G.N., Hamilton F.S., Hoppler S. Curr. Biol. 10:849-852(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Xenopus frizzled-7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation." Sumanas S., Ekker S.C. Genesis 30:119-122(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled." Wong H.C., Bourdelas A., Krauss A., Lee H.J., Shao Y., Wu D., Mlodzik M., Shi D.L., Zheng J. Mol. Cell 12:1251-1260(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DVL1, MUTAGENESIS OF LYS-526 AND TRP-531.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159106 mRNA. Translation: AAD44331.1. BC042228 mRNA. Translation: AAH42228.1.
UniGene	Xl.633.
3D structure databases
HSSP	HSSP built from PDB template 1IJY based on UniProtKB Q61091.
ProteinModelPortal	Q8AVJ9.
SMR	Q8AVJ9. Positions 34-151.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8AVJ9. 1 interaction.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Xenbase	XB-GENE-483735. fzd7.
Phylogenomic databases
HOVERGEN	HBG006977.
Family and domain databases
Gene3D	1.10.2000.10. 1 hit.
InterPro	IPR000539. Frizzled. IPR015526. Frizzled/SFRP. IPR020067. Frizzled_dom. IPR026552. FZD7. IPR017981. GPCR_2-like. [Graphical view]
PANTHER	PTHR11309. PTHR11309. 1 hit. PTHR11309:SF31. PTHR11309:SF31. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
SUPFAM	SSF63501. Frizzled_Cys-rich. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS00649. G_PROTEIN_RECEP_F2_1. False negative. PS00650. G_PROTEIN_RECEP_F2_2. False negative. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FZD7B_XENLA

Accession

Primary (citable) accession number: Q8AVJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents