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Protein

Frizzled-7-B

Gene

fzd7-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation.By similarity3 Publications

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. syndecan binding Source: UniProtKB
  3. Wnt-activated receptor activity Source: InterPro
  4. Wnt-protein binding Source: UniProtKB

GO - Biological processi

  1. convergent extension involved in gastrulation Source: UniProtKB
  2. dorsal/ventral axis specification Source: UniProtKB
  3. mesoderm formation Source: UniProtKB
  4. protein localization Source: UniProtKB
  5. tissue morphogenesis Source: UniProtKB
  6. Wnt signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-7-B
Short name:
Fz-7-B
Short name:
Xfz7-B
Gene namesi
Name:fzd7-b
Synonyms:fz7-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-483735. fzd7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 230212ExtracellularSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Topological domaini252 – 26211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei263 – 28321HelicalSequence AnalysisAdd
BLAST
Topological domaini284 – 31128ExtracellularSequence AnalysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence AnalysisAdd
BLAST
Topological domaini333 – 35321CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei354 – 37421HelicalSequence AnalysisAdd
BLAST
Topological domaini375 – 39723ExtracellularSequence AnalysisAdd
BLAST
Transmembranei398 – 41821HelicalSequence AnalysisAdd
BLAST
Topological domaini419 – 44426CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei445 – 46521HelicalSequence AnalysisAdd
BLAST
Topological domaini466 – 50237ExtracellularSequence AnalysisAdd
BLAST
Transmembranei503 – 52321HelicalSequence AnalysisAdd
BLAST
Topological domaini524 – 54825CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi526 – 5261K → M: Reduced binding to dvl1. 1 Publication
Mutagenesisi531 – 5311W → G: Reduced binding to dvl1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 548530Frizzled-7-BSequence AnalysisPRO_0000282949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 97PROSITE-ProRule annotation
Disulfide bondi44 ↔ 90PROSITE-ProRule annotation
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi81 ↔ 118PROSITE-ProRule annotation
Disulfide bondi107 ↔ 147PROSITE-ProRule annotation
Disulfide bondi111 ↔ 135PROSITE-ProRule annotation
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, localized to the cranial neural crest and heart field where expression is retained at later stages in addition to new areas of expression in the neural tube, pronephros and tailbud. At tailbud stage, expressed in the pronephric duct, and broad head expression becomes more restricted to the hindbrain. In tadpoles, strongly expressed in the eye and the pericardium and myocardium of the developing heart.1 Publication

Interactioni

Subunit structurei

Interacts with wnt11 and sdc4. The extracellular domain interacts with the extracellular domain of pcdh8/papc (By similarity). Interacts (via C-terminus) with dvl1 (via PDZ domain).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dvl1P511413EBI-3870271,EBI-1538407From a different organism.

Protein-protein interaction databases

IntActiQ8AVJ9. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8AVJ9.
SMRiQ8AVJ9. Positions 34-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 150120FZPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi526 – 5316Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members
Motifi546 – 5483PDZ-bindingSequence Analysis

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.By similarity
The extracellular domain interacts with Wnt proteins and the intracellular C-terminus transmits the Wnt signal.

Sequence similaritiesi

Belongs to the G-protein coupled receptor Fz/Smo family.Sequence Analysis
Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG006977.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8AVJ9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLAPVSLLFC LFLQLCPSAQ QYHGEKGISV PDHGFCQPIS IPLCTDIAYN
60 70 80 90 100
QTIMPNLLGH TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL
110 120 130 140 150
EQAIPPCRSL CERARQGCEA LMNKFGFQWP ERLRCENFPV HGAGEICVGQ
160 170 180 190 200
NTSDNSPSGP TARPTLNLPD SITFQPHPHR DFTCPRQLKV PPYLGYRFLG
210 220 230 240 250
EKDCGAPCEP GKANGLMYFK EEEVRFARLW VGIWAILCGI STLFTVLTYL
260 270 280 290 300
VDMRRFSYPE RPIIFLSGCY FMVAVAYTAG FLLEERAVCV ERFSEDSYRT
310 320 330 340 350
VAQGTKKEGC TILFMILYFF GMASSIWWVI LALTWFLSAG MKWGHEAIEA
360 370 380 390 400
NSQYFHLAAW AVPAVKTITI LAMGQVDGDV LSGVCYVGIN SVDSLRGFVL
410 420 430 440 450
APLFVYLFLG TSFLLAGFVS LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV
460 470 480 490 500
MYTVPATIVL ACYFYEQAFR DTWEKTWLVH TCKGYAVPCP NYNFAPMSPD
510 520 530 540
FTVFMIKYLM TMIVGITSSF WIWSGKTLQS WRRFYHRLGN GSKGETAV
Length:548
Mass (Da):61,906
Last modified:March 1, 2003 - v1
Checksum:i97B3408B3C0EA273
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681L → P in AAD44331. (PubMed:10446283)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159106 mRNA. Translation: AAD44331.1.
BC042228 mRNA. Translation: AAH42228.1.
UniGeneiXl.633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159106 mRNA. Translation: AAD44331.1.
BC042228 mRNA. Translation: AAH42228.1.
UniGeneiXl.633.

3D structure databases

ProteinModelPortaliQ8AVJ9.
SMRiQ8AVJ9. Positions 34-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8AVJ9. 1 interaction.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

XenbaseiXB-GENE-483735. fzd7.

Phylogenomic databases

HOVERGENiHBG006977.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel Xenopus frizzled genes expressed in developing heart and brain."
    Wheeler G.N., Hoppler S.
    Mech. Dev. 86:203-207(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: EmbryoImported.
  3. "Inducible gene expression in transgenic Xenopus embryos."
    Wheeler G.N., Hamilton F.S., Hoppler S.
    Curr. Biol. 10:849-852(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Xenopus frizzled-7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation."
    Sumanas S., Ekker S.C.
    Genesis 30:119-122(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled."
    Wong H.C., Bourdelas A., Krauss A., Lee H.J., Shao Y., Wu D., Mlodzik M., Shi D.L., Zheng J.
    Mol. Cell 12:1251-1260(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DVL1, MUTAGENESIS OF LYS-526 AND TRP-531.

Entry informationi

Entry nameiFZD7B_XENLA
AccessioniPrimary (citable) accession number: Q8AVJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.