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Q8AVJ9

- FZD7B_XENLA

UniProt

Q8AVJ9 - FZD7B_XENLA

Protein

Frizzled-7-B

Gene

fzd7-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation.By similarity3 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. syndecan binding Source: UniProtKB
    4. Wnt-activated receptor activity Source: InterPro
    5. Wnt-protein binding Source: UniProtKB

    GO - Biological processi

    1. convergent extension involved in gastrulation Source: UniProtKB
    2. dorsal/ventral axis specification Source: UniProtKB
    3. mesoderm formation Source: UniProtKB
    4. protein localization Source: UniProtKB
    5. tissue morphogenesis Source: UniProtKB
    6. Wnt signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Frizzled-7-B
    Short name:
    Fz-7-B
    Short name:
    Xfz7-B
    Gene namesi
    Name:fzd7-b
    Synonyms:fz7-b
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-483735. fzd7.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi526 – 5261K → M: Reduced binding to dvl1. 1 Publication
    Mutagenesisi531 – 5311W → G: Reduced binding to dvl1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 548530Frizzled-7-BSequence AnalysisPRO_0000282949Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi36 ↔ 97PROSITE-ProRule annotation
    Disulfide bondi44 ↔ 90PROSITE-ProRule annotation
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi81 ↔ 118PROSITE-ProRule annotation
    Disulfide bondi107 ↔ 147PROSITE-ProRule annotation
    Disulfide bondi111 ↔ 135PROSITE-ProRule annotation
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, localized to the cranial neural crest and heart field where expression is retained at later stages in addition to new areas of expression in the neural tube, pronephros and tailbud. At tailbud stage, expressed in the pronephric duct, and broad head expression becomes more restricted to the hindbrain. In tadpoles, strongly expressed in the eye and the pericardium and myocardium of the developing heart.1 Publication

    Interactioni

    Subunit structurei

    Interacts with wnt11 and sdc4. The extracellular domain interacts with the extracellular domain of pcdh8/papc By similarity. Interacts (via C-terminus) with dvl1 (via PDZ domain).By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dvl1P511413EBI-3870271,EBI-1538407From a different organism.

    Protein-protein interaction databases

    IntActiQ8AVJ9. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8AVJ9.
    SMRiQ8AVJ9. Positions 34-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 230212ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini252 – 26211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini284 – 31128ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini333 – 35321CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini375 – 39723ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini419 – 44426CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini466 – 50237ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini524 – 54825CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei263 – 28321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei312 – 33221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei354 – 37421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei398 – 41821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei445 – 46521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei503 – 52321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 150120FZPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi526 – 5316Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members
    Motifi546 – 5483PDZ-bindingSequence Analysis

    Domaini

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.By similarity
    The extracellular domain interacts with Wnt proteins and the intracellular C-terminus transmits the Wnt signal.

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor Fz/Smo family.Sequence Analysis
    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG006977.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026552. FZD7.
    IPR017981. GPCR_2-like.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF31. PTHR11309:SF31. 1 hit.
    PfamiPF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view]
    PRINTSiPR00489. FRIZZLED.
    SMARTiSM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8AVJ9-1 [UniParc]FASTAAdd to Basket

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    MLAPVSLLFC LFLQLCPSAQ QYHGEKGISV PDHGFCQPIS IPLCTDIAYN    50
    QTIMPNLLGH TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL 100
    EQAIPPCRSL CERARQGCEA LMNKFGFQWP ERLRCENFPV HGAGEICVGQ 150
    NTSDNSPSGP TARPTLNLPD SITFQPHPHR DFTCPRQLKV PPYLGYRFLG 200
    EKDCGAPCEP GKANGLMYFK EEEVRFARLW VGIWAILCGI STLFTVLTYL 250
    VDMRRFSYPE RPIIFLSGCY FMVAVAYTAG FLLEERAVCV ERFSEDSYRT 300
    VAQGTKKEGC TILFMILYFF GMASSIWWVI LALTWFLSAG MKWGHEAIEA 350
    NSQYFHLAAW AVPAVKTITI LAMGQVDGDV LSGVCYVGIN SVDSLRGFVL 400
    APLFVYLFLG TSFLLAGFVS LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV 450
    MYTVPATIVL ACYFYEQAFR DTWEKTWLVH TCKGYAVPCP NYNFAPMSPD 500
    FTVFMIKYLM TMIVGITSSF WIWSGKTLQS WRRFYHRLGN GSKGETAV 548
    Length:548
    Mass (Da):61,906
    Last modified:March 1, 2003 - v1
    Checksum:i97B3408B3C0EA273
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1681L → P in AAD44331. (PubMed:10446283)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159106 mRNA. Translation: AAD44331.1.
    BC042228 mRNA. Translation: AAH42228.1.
    UniGeneiXl.633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159106 mRNA. Translation: AAD44331.1 .
    BC042228 mRNA. Translation: AAH42228.1 .
    UniGenei Xl.633.

    3D structure databases

    ProteinModelPortali Q8AVJ9.
    SMRi Q8AVJ9. Positions 34-151.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8AVJ9. 1 interaction.

    Protein family/group databases

    GPCRDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Xenbasei XB-GENE-483735. fzd7.

    Phylogenomic databases

    HOVERGENi HBG006977.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026552. FZD7.
    IPR017981. GPCR_2-like.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF31. PTHR11309:SF31. 1 hit.
    Pfami PF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view ]
    PRINTSi PR00489. FRIZZLED.
    SMARTi SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two novel Xenopus frizzled genes expressed in developing heart and brain."
      Wheeler G.N., Hoppler S.
      Mech. Dev. 86:203-207(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: EmbryoImported.
    3. "Inducible gene expression in transgenic Xenopus embryos."
      Wheeler G.N., Hamilton F.S., Hoppler S.
      Curr. Biol. 10:849-852(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Xenopus frizzled-7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation."
      Sumanas S., Ekker S.C.
      Genesis 30:119-122(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled."
      Wong H.C., Bourdelas A., Krauss A., Lee H.J., Shao Y., Wu D., Mlodzik M., Shi D.L., Zheng J.
      Mol. Cell 12:1251-1260(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH DVL1, MUTAGENESIS OF LYS-526 AND TRP-531.

    Entry informationi

    Entry nameiFZD7B_XENLA
    AccessioniPrimary (citable) accession number: Q8AVJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3