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Q8AVJ9 (FZD7B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-7-B

Short name=Fz-7-B
Short name=Xfz7-B
Gene names
Name:fzd7-b
Synonyms:fz7-b
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation. Ref.3 Ref.4 Ref.5 UniProtKB Q9PUK8

Subunit structure

Interacts with wnt11 and sdc4. The extracellular domain interacts with the extracellular domain of pcdh8/papc By similarity. Interacts (via C-terminus) with dvl1 (via PDZ domain). Ref.5 UniProtKB Q9PUK8

Subcellular location

Membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, localized to the cranial neural crest and heart field where expression is retained at later stages in addition to new areas of expression in the neural tube, pronephros and tailbud. At tailbud stage, expressed in the pronephric duct, and broad head expression becomes more restricted to the hindbrain. In tadpoles, strongly expressed in the eye and the pericardium and myocardium of the developing heart. Ref.1

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

The FZ domain is involved in binding with Wnt ligands By similarity. UniProtKB Q9VVX3

The extracellular domain interacts with Wnt proteins and the intracellular C-terminus transmits the Wnt signal. UniProtKB Q9PUK8

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dvl1P511413EBI-3870271,EBI-1538407From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 548530Frizzled-7-B
PRO_0000282949

Regions

Topological domain19 – 230212Extracellular Potential
Transmembrane231 – 25121Helical; Potential
Topological domain252 – 26211Cytoplasmic Potential
Transmembrane263 – 28321Helical; Potential
Topological domain284 – 31128Extracellular Potential
Transmembrane312 – 33221Helical; Potential
Topological domain333 – 35321Cytoplasmic Potential
Transmembrane354 – 37421Helical; Potential
Topological domain375 – 39723Extracellular Potential
Transmembrane398 – 41821Helical; Potential
Topological domain419 – 44426Cytoplasmic Potential
Transmembrane445 – 46521Helical; Potential
Topological domain466 – 50237Extracellular Potential
Transmembrane503 – 52321Helical; Potential
Topological domain524 – 54825Cytoplasmic Potential
Domain31 – 150120FZ
Motif526 – 5316Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members
Motif546 – 5483PDZ-binding

Amino acid modifications

Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Disulfide bond36 ↔ 97 By similarity
Disulfide bond44 ↔ 90 By similarity
Disulfide bond81 ↔ 118 By similarity
Disulfide bond107 ↔ 147 By similarity
Disulfide bond111 ↔ 135 By similarity

Experimental info

Mutagenesis5261K → M: Reduced binding to dvl1. Ref.5
Mutagenesis5311W → G: Reduced binding to dvl1. Ref.5
Sequence conflict1681L → P in AAD44331. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8AVJ9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 97B3408B3C0EA273

FASTA54861,906
        10         20         30         40         50         60 
MLAPVSLLFC LFLQLCPSAQ QYHGEKGISV PDHGFCQPIS IPLCTDIAYN QTIMPNLLGH 

        70         80         90        100        110        120 
TNQEDAGLEV HQFYPLVKVQ CSPELRFFLC SMYAPVCTVL EQAIPPCRSL CERARQGCEA 

       130        140        150        160        170        180 
LMNKFGFQWP ERLRCENFPV HGAGEICVGQ NTSDNSPSGP TARPTLNLPD SITFQPHPHR 

       190        200        210        220        230        240 
DFTCPRQLKV PPYLGYRFLG EKDCGAPCEP GKANGLMYFK EEEVRFARLW VGIWAILCGI 

       250        260        270        280        290        300 
STLFTVLTYL VDMRRFSYPE RPIIFLSGCY FMVAVAYTAG FLLEERAVCV ERFSEDSYRT 

       310        320        330        340        350        360 
VAQGTKKEGC TILFMILYFF GMASSIWWVI LALTWFLSAG MKWGHEAIEA NSQYFHLAAW 

       370        380        390        400        410        420 
AVPAVKTITI LAMGQVDGDV LSGVCYVGIN SVDSLRGFVL APLFVYLFLG TSFLLAGFVS 

       430        440        450        460        470        480 
LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV MYTVPATIVL ACYFYEQAFR DTWEKTWLVH 

       490        500        510        520        530        540 
TCKGYAVPCP NYNFAPMSPD FTVFMIKYLM TMIVGITSSF WIWSGKTLQS WRRFYHRLGN 


GSKGETAV 

« Hide

References

« Hide 'large scale' references
[1]"Two novel Xenopus frizzled genes expressed in developing heart and brain."
Wheeler G.N., Hoppler S.
Mech. Dev. 86:203-207(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[3]"Inducible gene expression in transgenic Xenopus embryos."
Wheeler G.N., Hamilton F.S., Hoppler S.
Curr. Biol. 10:849-852(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Xenopus frizzled-7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation."
Sumanas S., Ekker S.C.
Genesis 30:119-122(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled."
Wong H.C., Bourdelas A., Krauss A., Lee H.J., Shao Y., Wu D., Mlodzik M., Shi D.L., Zheng J.
Mol. Cell 12:1251-1260(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH DVL1, MUTAGENESIS OF LYS-526 AND TRP-531.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF159106 mRNA. Translation: AAD44331.1.
BC042228 mRNA. Translation: AAH42228.1.
UniGeneXl.633.

3D structure databases

ProteinModelPortalQ8AVJ9.
SMRQ8AVJ9. Positions 34-151.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8AVJ9. 1 interaction.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

XenbaseXB-GENE-483735. fzd7.

Phylogenomic databases

HOVERGENHBG006977.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFZD7B_XENLA
AccessionPrimary (citable) accession number: Q8AVJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries