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Q8AVI9

- PORED_XENLA

UniProt

Q8AVI9 - PORED_XENLA

Protein

Polyprenol reductase

Gene

srd5a3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.By similarity

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
    2. dolichol metabolic process Source: UniProtKB
    3. polyprenol catabolic process Source: UniProtKB
    4. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase (EC:1.3.1.94)
    Alternative name(s):
    3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
    Steroid 5-alpha-reductase 3
    Short name:
    S5AR 3
    Short name:
    SR type 3
    Gene namesi
    Name:srd5a3
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-985517. srd5a3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Polyprenol reductasePRO_0000317705Add
    BLAST

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 7946LumenalSequence AnalysisAdd
    BLAST
    Topological domaini101 – 12222CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini144 – 15815LumenalSequence AnalysisAdd
    BLAST
    Topological domaini180 – 19516CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini217 – 26650LumenalSequence AnalysisAdd
    BLAST
    Topological domaini288 – 31932CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei80 – 10021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG057797.
    KOiK12345.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8AVI9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLVQLLPPG VSLLALVWLA VDAAFLTALL LYLQRGCDSG RSLLCSVFQD    50
    LIRYGKTKSG LRRPSWLQWF DIPKRCFWHF YFVSLVWNGF LLWILLHLLL 100
    QSVPVPEWLQ AVLQFLCAGS EPQVLGGELS VVLAFSLLWL HSLRRLLECL 150
    FVSIFSNGVI HFVQYCFGLG YYILIGFTIL GYCPLDRRTA VSLDDLLMQG 200
    NWYHILGLTL YVWASLHQYT CHCILADLRK SASGAIINLK HAVPTGDWFE 250
    KVSCPHYFAE LLIYLSIAVV FGLLNTIWWL VVLYVLLSQA LAAVLCHEFY 300
    HEKFDSYPIH RKAFIPLIF 319
    Length:319
    Mass (Da):36,591
    Last modified:March 1, 2003 - v1
    Checksum:i5163DCD07643B1DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC042255 mRNA. Translation: AAH42255.1.
    RefSeqiNP_001079436.1. NM_001085967.1.
    UniGeneiXl.15326.

    Genome annotation databases

    GeneIDi379123.
    KEGGixla:379123.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC042255 mRNA. Translation: AAH42255.1 .
    RefSeqi NP_001079436.1. NM_001085967.1.
    UniGenei Xl.15326.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 379123.
    KEGGi xla:379123.

    Organism-specific databases

    CTDi 79644.
    Xenbasei XB-GENE-985517. srd5a3.

    Phylogenomic databases

    HOVERGENi HBG057797.
    KOi K12345.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.

    Entry informationi

    Entry nameiPORED_XENLA
    AccessioniPrimary (citable) accession number: Q8AVI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3