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Q8AVI9 (PORED_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprenol reductase

EC=1.3.1.94
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3
EC=1.3.1.22
Steroid 5-alpha-reductase 3
Short name=S5AR 3
Short name=SR type 3
Gene names
Name:srd5a3
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Polyprenol reductase
PRO_0000317705

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Potential
Topological domain34 – 7946Lumenal Potential
Transmembrane80 – 10021Helical; Potential
Topological domain101 – 12222Cytoplasmic Potential
Transmembrane123 – 14321Helical; Potential
Topological domain144 – 15815Lumenal Potential
Transmembrane159 – 17921Helical; Potential
Topological domain180 – 19516Cytoplasmic Potential
Transmembrane196 – 21621Helical; Potential
Topological domain217 – 26650Lumenal Potential
Transmembrane267 – 28721Helical; Potential
Topological domain288 – 31932Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
Q8AVI9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5163DCD07643B1DB

FASTA31936,591
        10         20         30         40         50         60 
MQLVQLLPPG VSLLALVWLA VDAAFLTALL LYLQRGCDSG RSLLCSVFQD LIRYGKTKSG 

        70         80         90        100        110        120 
LRRPSWLQWF DIPKRCFWHF YFVSLVWNGF LLWILLHLLL QSVPVPEWLQ AVLQFLCAGS 

       130        140        150        160        170        180 
EPQVLGGELS VVLAFSLLWL HSLRRLLECL FVSIFSNGVI HFVQYCFGLG YYILIGFTIL 

       190        200        210        220        230        240 
GYCPLDRRTA VSLDDLLMQG NWYHILGLTL YVWASLHQYT CHCILADLRK SASGAIINLK 

       250        260        270        280        290        300 
HAVPTGDWFE KVSCPHYFAE LLIYLSIAVV FGLLNTIWWL VVLYVLLSQA LAAVLCHEFY 

       310 
HEKFDSYPIH RKAFIPLIF 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC042255 mRNA. Translation: AAH42255.1.
RefSeqNP_001079436.1. NM_001085967.1.
UniGeneXl.15326.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID379123.
KEGGxla:379123.

Organism-specific databases

CTD79644.
XenbaseXB-GENE-985517. srd5a3.

Phylogenomic databases

HOVERGENHBG057797.
KOK12345.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePORED_XENLA
AccessionPrimary (citable) accession number: Q8AVI9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways