Reviewed, UniProtKB/Swiss-Prot Q8AVF5 (CMAH_XENLA)

Names and origin

Protein names

Recommended name:
    Cytidine monophosphate-N-acetylneuraminic acid hydroxylase
      Short name=CMP-N-acetylneuraminic acid hydroxylase
    EC=1.14.18.2
Alternative name(s):
    CMP-Neu5Ac hydroxylase
    CMP-NeuAc hydroxylase
    CMP-N-acetylneuraminate monooxygenase

Gene names

Name:

cmah

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells By similarity.
Catalytic activity	CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ = CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Pathway	Amino-sugar metabolism; N-acetylneuraminic acid metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the CMP-Neu5Ac hydroxylase family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cytoplasm
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CMP-N-acetylneuraminate monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Cytidine monophosphate-N-acetylneuraminic acid hydroxylase		PRO_0000127809
Regions
	Domain	16 – 114	99	Rieske
Sites
	Metal binding	56	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	58	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	77	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	80	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8AVF5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2051BEB6807E988C	FASTA	591	68,369
	10 20 30 40 50 60 MEQSNDGQTA HTLLHLASAE VESLKEGITF LRNKESGKNF IIYKNGEELR ACKNLCKHQG 70 80 90 100 110 120 GTFIKDIEDL GNRTVRCTKH NWKLDVSSMK YVNPPDSFCQ DELVIENDDE NGVSLVELSP 130 140 150 160 170 180 PNPWDSDPRM AVCLEGGEVQ VTYLTHACMD LKLGNKHMVF DPWLIGPAFA RGWWLLHEPP 190 200 210 220 230 240 CDWLERLCRA DLIYISHMHS DHLSYPTLKK LSEKRPDIPI YVGKTERPVF WYLDKSGVKL 250 260 270 280 290 300 TNINVVPFGI WQEVDENLRF MILMDGVHPE MDTCIIVEYK GNKILNTVDC TRPNGGKLPT 310 320 330 340 350 360 NVALMMSDFA GGASGFPMTF SGGKFTEEWK SQFIKTERKK LLNYKAQLVK DLNPRIYCPF 370 380 390 400 410 420 AGYFVEEHPS DKYIKETNLK NDAAELNMLI RNTSDVVTWT PKPGAILDLG RLLIDPTDKN 430 440 450 460 470 480 GIIDPPPGTK IFKDSWDYDT YLSIHSFSFD DEIFHYPSWI KEYFTWAGFK GYNLVLRMIE 490 500 510 520 530 540 TDEHFVPLPK GYNYLVDFLD LSFPTERPER DHPYEEISSR ATVIRHVVKH GLLWDDLYIG 550 560 570 580 590 FQTRIQRNPD IYHHQFWNHF QIKLPLTPPD WKVFLDREKE NNATLQNCSI M

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC042338 mRNA. Translation: AAH42338.1.
RefSeq	NP_001080297.1.
UniGene	Xl.76765
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	379989.
KEGG	xla:379989.
Organism-specific databases
Xenbase	XB-FEAT-988334. cmah.
Phylogenomic databases
HOVERGEN	Q8AVF5.
Enzyme and pathway databases
BRENDA	1.14.18.2. 648.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. [Graphical view]
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMAH_XENLA

Accession

Primary (citable) accession number: Q8AVF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project