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Protein

Cysteine-rich venom protein pseudecin

Gene
N/A
Organism
Pseudechis porphyriacus (Red-bellied black snake)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Blocks olfactory (CNGA2) and retinal (CNGA1) CNG channel currents. Is really less potent that Pseudechetoxin. Does not affect neither depolarization- nor caffeine-induced contraction of smooth muscle.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Zinc1 Publication
Metal bindingi106 – 1061Zinc1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich venom protein pseudecin
Short name:
CRVP Pdc
OrganismiPseudechis porphyriacus (Red-bellied black snake)
Taxonomic identifieri8671 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeAcanthophiinaePseudechis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Propeptidei20 – 2891 PublicationPRO_0000006284
Chaini29 – 238210Cysteine-rich venom protein pseudecinPRO_0000006285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 153PROSITE-ProRule annotation1 Publication
Disulfide bondi92 ↔ 165PROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 162PROSITE-ProRule annotation1 Publication
Disulfide bondi184 ↔ 191PROSITE-ProRule annotation1 Publication
Disulfide bondi187 ↔ 196PROSITE-ProRule annotation1 Publication
Disulfide bondi200 ↔ 233PROSITE-ProRule annotation1 Publication
Disulfide bondi209 ↔ 227PROSITE-ProRule annotation1 Publication
Disulfide bondi218 ↔ 231PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
238
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 4513Combined sources
Beta strandi52 – 543Combined sources
Helixi62 – 7211Combined sources
Turni73 – 753Combined sources
Helixi82 – 843Combined sources
Beta strandi85 – 873Combined sources
Beta strandi93 – 1019Combined sources
Helixi105 – 1139Combined sources
Helixi114 – 1185Combined sources
Turni121 – 1233Combined sources
Beta strandi124 – 1274Combined sources
Helixi133 – 1386Combined sources
Beta strandi145 – 15410Combined sources
Beta strandi157 – 16610Combined sources
Beta strandi168 – 1703Combined sources
Beta strandi180 – 1823Combined sources
Turni183 – 1864Combined sources
Beta strandi190 – 1923Combined sources
Helixi209 – 2168Combined sources
Helixi221 – 2266Combined sources
Helixi228 – 2325Combined sources
Beta strandi234 – 2374Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DDBX-ray1.90A/B/C/D29-238[»]
2EPFX-ray2.30A/B/C/D29-238[»]
ProteinModelPortaliQ8AVA3.
SMRiQ8AVA3. Positions 30-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8AVA3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 164127SCPAdd
BLAST
Domaini200 – 23334ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004184.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8AVA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAFIVLLSL AAVLQQSSGT VDFASESSNK KNYQKEIVDK HNALRRSVKP
60 70 80 90 100
TARNMLQMKW NSHAAQNAKR WADRCTFAHS PPNTRTVGKL RCGENIFMSS
110 120 130 140 150
QPFPWSGVVQ AWYDEIKNFV YGIGAKPPGS VIGHYTQVVW YKSHLIGCAS
160 170 180 190 200
AKCSSSKYLY VCQYCPAGNI RGSIATPYKS GPPCADCPSA CVNRLCTNPC
210 220 230
NYNNDFSNCK SLAKKSKCQT EWIKKKCPAS CFCHNKII
Length:238
Mass (Da):26,452
Last modified:March 1, 2003 - v1
Checksum:i0EE4018E1274D85B
GO

Mass spectrometryi

Molecular mass is 23651.9±82.8 Da from positions 29 - 238. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072696 mRNA. Translation: AAL65292.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072696 mRNA. Translation: AAL65292.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DDBX-ray1.90A/B/C/D29-238[»]
2EPFX-ray2.30A/B/C/D29-238[»]
ProteinModelPortaliQ8AVA3.
SMRiQ8AVA3. Positions 30-238.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004184.

Miscellaneous databases

EvolutionaryTraceiQ8AVA3.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and cloning of toxins from elapid venoms that target cyclic nucleotide-gated ion channels."
    Yamazaki Y., Brown R.L., Morita T.
    Biochemistry 41:11331-11337(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-34, FUNCTION, MASS SPECTROMETRY.
    Tissue: Venom and Venom gland.
  2. "Crystallization and preliminary X-ray diffraction analyses of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels."
    Suzuki N., Yamazaki Y., Fujimoto Z., Morita T., Mizuno H.
    Acta Crystallogr. F 61:750-752(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  3. "Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels: implications for movement of the C-terminal cysteine-rich domain."
    Suzuki N., Yamazaki Y., Brown R.L., Fujimoto Z., Morita T., Mizuno H.
    Acta Crystallogr. D 64:1034-1042(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 29-238 ALONE AND IN COMPLEX WITH ZINC, ZINC BINDING SITES, DISULFIDE BONDS.

Entry informationi

Entry nameiCRVP_PSEPO
AccessioniPrimary (citable) accession number: Q8AVA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 1, 2003
Last modified: October 14, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.