Q8AAP2 (SYT_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BT_0422

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100940
Regions
	Region	242 – 541	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	337	1	Zinc; catalytic By similarity
	Metal binding	388	1	Zinc; catalytic By similarity
	Metal binding	518	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8AAP2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F4ABDFA10DA9C500	FASTA	646	74,553
	10 20 30 40 50 60 MIKITFPDGS VREYNEGVNG LQIAESISSR LAQDVLACGV NGETYDLGRP INEDADFVLY 70 80 90 100 110 120 KWDDEEGKHA FWHTSAHLLA EALQELYPGI QFGIGPAIEN GFYYDVDPGE AVIKESDLPA 130 140 150 160 170 180 IEAKMLELSA KKDAVVRESI SKTDALKMFG DRGETYKCEL ISELEDGHIT TYTQGAFTDL 190 200 210 220 230 240 CRGPHLMTTA PIKAIKLTTV AGAYWRGHED RKMLTRIYGI TFPKKKMLDE YLVLLEEAKK 250 260 270 280 290 300 RDHRKIGKEM QLFMFSETVG KGLPMWLPKG TALRLRLQEF LRRIQTRYDY QEVITPPIGN 310 320 330 340 350 360 KLLYVTSGHY AKYGKDAFQP IHTPEEGEEY FLKPMNCPHH CEIYKNFPRS YKDLPLRIAE 370 380 390 400 410 420 FGTVCRYEQS GELHGLTRVR SFTQDDAHIF CRPDQVKDEF LRVMDIISIV FRSMNFQNFE 430 440 450 460 470 480 AQISLRDKVN REKYIGSDDN WEKAEQAIIE ACEEKGLPAK IEYGEAAFYG PKLDFMVKDA 490 500 510 520 530 540 IGRRWQLGTI QVDYNLPERF ELEYMGSDNQ KHRPVMIHRA PFGSMERFVA VLIEHTAGKF 550 560 570 580 590 600 PLWLTPDQVA ILPISEKFNE YAEQVKMYLK MHEIRAIVDD RNEKIGRKIR DNEMKRIPYM 610 620 630 640 LIVGEKEAEN GEVSVRRQGE GDKGTMKYEE FAKILNEEVQ NMINKW

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO75529.1.
RefSeq	NP_809335.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8AAP2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1074753.
GenomeReviews	Gene locus BT_0422 in contig AE015928_GR.
KEGG	bth:BT_0422.
NMPDR	fig|226186.1.peg.422.
PATRIC	21055587. VBIBacThe70966_0421.
Phylogenomic databases
HOGENOM	HBG352811.
OMA	GRKWQLG.
PhylomeDB	Q8AAP2.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_0422-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BACTN

Accession

Primary (citable) accession number: Q8AAP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program