Q8AAK6 (Q8AAK6_BACTN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-mannosidase EMBL AAO75565.1

Gene names

Ordered Locus Names:

BT_0458

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2WBK PDB 2JE8 PDB 2VQU PDB 2VMF PDB 2VL4 PDB 2VOT PDB 2VQT PDB 2VR4 PDB 2VO5 PDB 2VJX Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8AAK6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F6EB7577A9E5EFA1	FASTA	864	99,506
	10 20 30 40 50 60 MILNLTGKIA PIACGLLCCC SMVYAQGNDT SEVMLLDTGW EFSQSGTEKW MPATVPGTVH 70 80 90 100 110 120 QDLISHELLP NPFYGMNEKK IQWVENEDWE YRTSFIVSEE QLNRDGIQLI FEGLDTYADV 130 140 150 160 170 180 YLNGSLLLKA DNMFVGYTLP VKSVLRKGEN HLYIYFHSPI RQTLPQYASN GFNYPADNDH 190 200 210 220 230 240 HEKHLSVFSR KAPYSYGWDW GIRMVTSGVW RPVTLRFYDI ATISDYYVRQ LSLTDENARL 250 260 270 280 290 300 SNELIVNQIV PQKIPAEVRV NVSLNGTTVT EVKQQVTLQP GINHITLPAE VTNPVRWMPN 310 320 330 340 350 360 GWGTPTLYDF SAQIACGDRI VAEQSHRIGL RTIRVVNEKD KDGESFYFEV NGIPMFAKGA 370 380 390 400 410 420 NYIPQDALLP NVTTERYQTL FRDMKEANMN MVRIWGGGTY ENNLFYDLAD ENGILVWQDF 430 440 450 460 470 480 MFACTPYPSD PTFLKRVEAE AVYNIRRLRN HASLAMWCGN NEILEALKYW GFEKKFTPEV 490 500 510 520 530 540 YQGLMHGYDK LFRELLPSTV KEFDSDRFYV HSSPYLANWG RPESWGTGDS HNWGVWYGKK 550 560 570 580 590 600 PFESLDTDLP RFMSEFGFQS FPEMKTIAAF AAPEDYQIES EVMNAHQKSS IGNSLIRTYM 610 620 630 640 650 660 ERDYIIPESF EDFVYVGLVL QGQGMRHGLE AHRRNRPYCM GTLYWQLNDS WPVVSWSSID 670 680 690 700 710 720 YYGNWKALHY QAKRAFAPVL INPIQQNDSL SVYLISDRLD TMEQMTLEMK VVDFDGKTLG 730 740 750 760 770 780 KKIQVHSLEV PANTSKCVYR AKLDGWLTPE DCRRSFLKLI LKDKSGHQVA ESVHFFRKTK 790 800 810 820 830 840 DLQLPPTSVS YQMKQTDGKC ELTLFSSMLA KDIFIETPLQ GARYSDNFFD LLPGERKKVI 850 860 ITSPRIKKGE ELPVNIKHIR ETYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.
[2]	"Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis." Offen W.A., Zechel D.L., Withers S.G., Gilbert H.J., Davies G.J. Chem. Commun. (Camb.) 2009:2484-2486(2009) [PubMed: 19532864] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 26-864.
[3]	"Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A." Tailford L.E., Money V.A., Smith N.L., Dumon C., Davies G.J., Gilbert H.J. J. Biol. Chem. 282:11291-11299(2007) [PubMed: 17287210] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 26-864.
[4]	"Structural and biochemical evidence for a boat-like transition state in beta-mannosidases." Tailford L.E., Offen W.A., Smith N.L., Dumon C., Morland C., Gratien J., Heck M.P., Stick R.V., Bleriot Y., Vasella A., Gilbert H.J., Davies G.J. Nat. Chem. Biol. 4:306-312(2008) [PubMed: 18408714] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-864.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE015928 Genomic DNA. Translation: AAO75565.1.

RefSeq

NP_809371.1. NC_004663.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JE8	X-ray	1.70	A/B	26-864	[»]
2VJX	X-ray	1.85	A/B	26-864	[»]
2VL4	X-ray	1.90	A/B	26-864	[»]
2VMF	X-ray	2.10	A/B	26-864	[»]
2VO5	X-ray	2.30	A/B	26-864	[»]
2VOT	X-ray	1.95	A/B	26-864	[»]
2VQT	X-ray	2.10	A/B	26-864	[»]
2VQU	X-ray	1.90	A/B	26-864	[»]
2VR4	X-ray	1.80	A/B	26-864	[»]
2WBK	X-ray	2.10	A/B	26-864	[»]

ProteinModelPortal

Q8AAK6.

SMR

Q8AAK6. Positions 27-864.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1071631.

GenomeReviews

Gene locus BT_0458 in contig AE015928_GR.

KEGG

bth:BT_0458.

NMPDR

fig|226186.1.peg.458.

PATRIC

21055663. VBIBacThe70966_0458.

Phylogenomic databases

HOGENOM

HBG584951.

OMA

AIGWYDE.

ProtClustDB

CLSK822193.

Family and domain databases

InterPro

IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

KO

K01192.

Pfam

PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q8AAK6_BACTN

Accession

Primary (citable) accession number: Q8AAK6

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)