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Protein
Submitted name:

Beta-mannosidase

Gene

BT_0458

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-460-MONOMER.
BRENDAi3.2.1.25. 709.

Names & Taxonomyi

Protein namesi
Submitted name:
Beta-mannosidaseImported
Gene namesi
Ordered Locus Names:BT_0458Imported
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)Imported
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 864839Sequence analysisPRO_5004303097Add
BLAST

Proteomic databases

PaxDbiQ8AAK6.

Interactioni

Protein-protein interaction databases

STRINGi226186.BT_0458.

Chemistry

BindingDBiQ8AAK6.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JE8X-ray1.70A/B26-864[»]
2VJXX-ray1.85A/B26-864[»]
2VL4X-ray1.90A/B26-864[»]
2VMFX-ray2.10A/B26-864[»]
2VO5X-ray2.30A/B26-864[»]
2VOTX-ray1.95A/B26-864[»]
2VQTX-ray2.10A/B26-864[»]
2VQUX-ray1.90A/B26-864[»]
2VR4X-ray1.80A/B26-864[»]
2WBKX-ray2.10A/B26-864[»]
ProteinModelPortaliQ8AAK6.
SMRiQ8AAK6. Positions 27-864.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8AAK6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 331110Glyco_hydro_2InterPro annotationAdd
BLAST

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiENOG4105D6I. Bacteria.
COG3250. LUCA.
InParanoidiQ8AAK6.
KOiK01192.
OMAiALATNWF.
OrthoDBiEOG6VHZ94.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8AAK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILNLTGKIA PIACGLLCCC SMVYAQGNDT SEVMLLDTGW EFSQSGTEKW
60 70 80 90 100
MPATVPGTVH QDLISHELLP NPFYGMNEKK IQWVENEDWE YRTSFIVSEE
110 120 130 140 150
QLNRDGIQLI FEGLDTYADV YLNGSLLLKA DNMFVGYTLP VKSVLRKGEN
160 170 180 190 200
HLYIYFHSPI RQTLPQYASN GFNYPADNDH HEKHLSVFSR KAPYSYGWDW
210 220 230 240 250
GIRMVTSGVW RPVTLRFYDI ATISDYYVRQ LSLTDENARL SNELIVNQIV
260 270 280 290 300
PQKIPAEVRV NVSLNGTTVT EVKQQVTLQP GINHITLPAE VTNPVRWMPN
310 320 330 340 350
GWGTPTLYDF SAQIACGDRI VAEQSHRIGL RTIRVVNEKD KDGESFYFEV
360 370 380 390 400
NGIPMFAKGA NYIPQDALLP NVTTERYQTL FRDMKEANMN MVRIWGGGTY
410 420 430 440 450
ENNLFYDLAD ENGILVWQDF MFACTPYPSD PTFLKRVEAE AVYNIRRLRN
460 470 480 490 500
HASLAMWCGN NEILEALKYW GFEKKFTPEV YQGLMHGYDK LFRELLPSTV
510 520 530 540 550
KEFDSDRFYV HSSPYLANWG RPESWGTGDS HNWGVWYGKK PFESLDTDLP
560 570 580 590 600
RFMSEFGFQS FPEMKTIAAF AAPEDYQIES EVMNAHQKSS IGNSLIRTYM
610 620 630 640 650
ERDYIIPESF EDFVYVGLVL QGQGMRHGLE AHRRNRPYCM GTLYWQLNDS
660 670 680 690 700
WPVVSWSSID YYGNWKALHY QAKRAFAPVL INPIQQNDSL SVYLISDRLD
710 720 730 740 750
TMEQMTLEMK VVDFDGKTLG KKIQVHSLEV PANTSKCVYR AKLDGWLTPE
760 770 780 790 800
DCRRSFLKLI LKDKSGHQVA ESVHFFRKTK DLQLPPTSVS YQMKQTDGKC
810 820 830 840 850
ELTLFSSMLA KDIFIETPLQ GARYSDNFFD LLPGERKKVI ITSPRIKKGE
860
ELPVNIKHIR ETYK
Length:864
Mass (Da):99,506
Last modified:June 1, 2003 - v1
Checksum:iF6EB7577A9E5EFA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO75565.1.
RefSeqiNP_809371.1. NC_004663.1.
WP_011107276.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO75565; AAO75565; BT_0458.
GeneIDi1071631.
KEGGibth:BT_0458.
PATRICi21055663. VBIBacThe70966_0458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO75565.1.
RefSeqiNP_809371.1. NC_004663.1.
WP_011107276.1. NC_004663.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JE8X-ray1.70A/B26-864[»]
2VJXX-ray1.85A/B26-864[»]
2VL4X-ray1.90A/B26-864[»]
2VMFX-ray2.10A/B26-864[»]
2VO5X-ray2.30A/B26-864[»]
2VOTX-ray1.95A/B26-864[»]
2VQTX-ray2.10A/B26-864[»]
2VQUX-ray1.90A/B26-864[»]
2VR4X-ray1.80A/B26-864[»]
2WBKX-ray2.10A/B26-864[»]
ProteinModelPortaliQ8AAK6.
SMRiQ8AAK6. Positions 27-864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_0458.

Chemistry

BindingDBiQ8AAK6.

Proteomic databases

PaxDbiQ8AAK6.

Protocols and materials databases

DNASUi1071631.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO75565; AAO75565; BT_0458.
GeneIDi1071631.
KEGGibth:BT_0458.
PATRICi21055663. VBIBacThe70966_0458.

Phylogenomic databases

eggNOGiENOG4105D6I. Bacteria.
COG3250. LUCA.
InParanoidiQ8AAK6.
KOiK01192.
OMAiALATNWF.
OrthoDBiEOG6VHZ94.

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-460-MONOMER.
BRENDAi3.2.1.25. 709.

Miscellaneous databases

EvolutionaryTraceiQ8AAK6.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482Imported.
  2. "Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A."
    Tailford L.E., Money V.A., Smith N.L., Dumon C., Davies G.J., Gilbert H.J.
    J. Biol. Chem. 282:11291-11299(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 26-864.
  3. "Structural and biochemical evidence for a boat-like transition state in beta-mannosidases."
    Tailford L.E., Offen W.A., Smith N.L., Dumon C., Morland C., Gratien J., Heck M.P., Stick R.V., Bleriot Y., Vasella A., Gilbert H.J., Davies G.J.
    Nat. Chem. Biol. 4:306-312(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-864.
  4. "Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis."
    Offen W.A., Zechel D.L., Withers S.G., Gilbert H.J., Davies G.J.
    Chem. Commun. (Camb.) 2009:2484-2486(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 26-864.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482Imported.

Entry informationi

Entry nameiQ8AAK6_BACTN
AccessioniPrimary (citable) accession number: Q8AAK6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2003
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.