Q8A9S8 (FTHS_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	BT_0737

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › Bacteroides thetaiotaomicron

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199335
Regions
	Nucleotide binding	64 – 71	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A9S8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2E9B6E45357D0F8D	FASTA	555	60,482
	10 20 30 40 50 60 MKSDIEIARS VELKKIKQVA ESIGIPREEV ENYGRYIAKI PEQLIDEEKV KKSNLVLVTA 70 80 90 100 110 120 ITATKAGIGK TTVSIGLALG LNKIGKKAIV ALREPSLGPC FGMKGGAAGG GYAQVLPMEK 130 140 150 160 170 180 INLHFTGDFH AITSAHNMIS ALLDNYLYQN QAKGFGLKEI LWRRVLDVND RSLRSIVVGL 190 200 210 220 230 240 GPKSNGITQE SGFDITPASE IMAILCLSKD VEDLRRRIEN ILLGFTYDDQ PFTVKDLGVA 250 260 270 280 290 300 GAITVLLKDA IHPNLVQTTE GTAAFVHGGP FANIAHGCNS ILATKLAMSF GDYVITEAGF 310 320 330 340 350 360 GADLGAEKFY NIKCRKSGLQ PKLTVIVATA QGLKMHGGVS LDRIKEPNME GLKEGLRNLD 370 380 390 400 410 420 KHIRNLRSFG QTVVVAFNKF ATDTDEEMEM LREHCEQLGV GYAINNAFSD GGDGAVDMAR 430 440 450 460 470 480 LVVDTIENNP SEPLRYTYKE EDSIQQKIEK VATNLYGASV ITYSSIARNR IKLIEKMGIT 490 500 510 520 530 540 HYPVCIAKTQ YSFSADPKIY GAVNNFEFHI KDIVINNGAE MIVAIAGEIL RMPGLPKEPQ 550 ALHIDIVDGE IEGLS

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO75844.1.
RefSeq	NP_809650.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A9S8.
SMR	Q8A9S8. Positions 4-553.
ModBase	Search...
Protein-protein interaction databases
STRING	226186.BT_0737.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO75844; AAO75844; BT_0737.
GeneID	1073612.
KEGG	bth:BT_0737.
PATRIC	21056263. VBIBacThe70966_0752.
Phylogenomic databases
eggNOG	COG2759.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	BTHE226186:GJXV-745-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR027000. FHS_archaeal_bac. IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
PANTHER	PTHR10025:SF20. PTHR10025:SF20. 1 hit.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_BACTN

Accession

Primary (citable) accession number: Q8A9S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program