Q8A9S7 (GLYA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	BT_0738

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051 Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Pyridoxal-phosphate-dependent serine hydroxymethyltransferase		PRO_0000113533
Regions
	Region	117 – 119	3	Substrate binding By similarity
Sites
	Binding site	27	1	Pyridoxal phosphate By similarity
	Binding site	47	1	Pyridoxal phosphate By similarity
	Binding site	49	1	Substrate By similarity
	Binding site	56	1	Substrate binding By similarity
	Binding site	57	1	Pyridoxal phosphate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	168	1	Pyridoxal phosphate By similarity
	Binding site	196	1	Pyridoxal phosphate By similarity
	Binding site	221	1	Pyridoxal phosphate By similarity
	Binding site	228	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	371	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	222	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A9S7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B815386EA1763FFF	FASTA	426	46,776
	10 20 30 40 50 60 MKRDDLIFDI IEKEHQRQLK GIELIASENF VSDQVMQAMG SCLTNKYAEG YPGKRYYGGC 70 80 90 100 110 120 EVVDQSEQIA IDRLKEIFGA EWANVQPHSG AQANAAVFLA VLNPGDKFMG LNLAHGGHLS 130 140 150 160 170 180 HGSLVNTSGI IYTPCEYNLN QETGRVDYDQ MEEVALREKP KMIIGGGSAY SREWDYKRMR 190 200 210 220 230 240 EIADKVGAIL MIDMAHPAGL IAAGLLENPV KYAHIVTSTT HKTLRGPRGG VIMMGKDFPN 250 260 270 280 290 300 PWGKKTPKGE IKMMSQLLDS AVFPGVQGGP LEHVIAAKAV AFGEILQPEY KEYAKQVQKN 310 320 330 340 350 360 AAILAQALID RGFTIVSGGT DNHSMLVDLR SKYPDLTGKV AEKALVSADI TVNKNMVPFD 370 380 390 400 410 420 SRSAFQTSGI RLGTPAITTR GAKEDLMIEI AEMIETVLSN VENEEVIAQV RARVNETMKK YPLFAD

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO75845.1.
RefSeq	NP_809651.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A9S7.
SMR	Q8A9S7. Positions 2-418.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1073605.
GenomeReviews	Gene locus BT_0738 in contig AE015928_GR.
KEGG	bth:BT_0738.
NMPDR	fig|226186.1.peg.738.
PATRIC	21056265. VBIBacThe70966_0753.
Phylogenomic databases
HOGENOM	HBG301263.
OMA	GTSNHLM.
PhylomeDB	Q8A9S7.
ProtClustDB	PRK00011.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_0738-MONOMER.
Family and domain databases
HAMAP	MF_00051. SHMT. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00600.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_BACTN

Accession

Primary (citable) accession number: Q8A9S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program