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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei713 – 7131ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-815-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BT_0806
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11621162Isoleucine--tRNA ligasePRO_0000098522Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_0806.

Structurei

3D structure databases

ProteinModelPortaliQ8A9K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi710 – 7145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ8A9K9.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8A9K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRFTEYSQ FDLSQVNKDV LKKWDENQVF AKSMTERDGC PSFVFFEGPP
60 70 80 90 100
SANGMPGIHH VMARTIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELSVEK
110 120 130 140 150
ALGITKEDIG KKISVADYNA ACRKDVMKYT KEWEDLTHQM GYWVDMKHPY
160 170 180 190 200
ITYDNRYIET LWWLLKQLHK KGLLYKGYTI QPYSPAAGTG LSSHELNQPG
210 220 230 240 250
CYRDVKDTTA VAQFKMKNPK PEMAEWGTPY FLAWTTTPWT LPSNTALCVG
260 270 280 290 300
PKIDYVAVQT YNAYTGEPIT VVLAKALLNT HFNSKAADLK LEDYKAGDKL
310 320 330 340 350
VPFKVVAEYK GADLIGMEYE QLIPWVKPVE VSEDGTWKVS GKGFRVIPGD
360 370 380 390 400
YVTTEDGTGI VHIAPTFGAD DANVARAAGI PSLFMINKKG ETRPMVDLTG
410 420 430 440 450
KFYMLDELDE NFVKECVDVD KYKEYQGAWV KNAYNPVFMV DGKYDEKAAQ
460 470 480 490 500
AAESLDVALC MMMKANNQAF KIEKHIHNYP HCWRTDKPVL YYPLDSWFIR
510 520 530 540 550
STACKERMME LNKTINWKPE STGTGRFGKW LENLNDWNLS RSRYWGTPLP
560 570 580 590 600
IWRTEDGTSE ICIESVEELY NEIEKSVAAG FMKSNPYKDK GFVPGEYTEG
610 620 630 640 650
NYDKIDLHRP YVDDIILVSE DGQPMKRESD LIDVWFDSGA MPYAQIHYPF
660 670 680 690 700
ENKNILDNRE VYPADFIAEG VDQTRGWFFT LHAIATMVFD SVSYKAVISN
710 720 730 740 750
GLVLDKNGNK MSKRLNNAVD PFTTIEKYGS DPLRWYMITN SSPWDNLKFD
760 770 780 790 800
IDGIEEVRRK FFGTLYNTYS FFALYANVDG FEYKEADVPM AERPEIDRWI
810 820 830 840 850
LSVLNTLIKE VDTCYNEYEP TKAGRLISDF VNDNLSNWYV RLNRKRFWGG
860 870 880 890 900
EFTQDKLSAY QTLYTCLETV AKLMAPISPF YADRLYTDLT TATGRDNVVS
910 920 930 940 950
IHLAEFPKYQ EEMIDKELEA RMQMAQDVTS MVLALRRKVN IKVRQPLQCI
960 970 980 990 1000
MIPVADEEQK AHIEAVKALI MNEVNVKDIK FVDGAAGVLV KKVKCDFKKL
1010 1020 1030 1040 1050
GPKFGKQMKA VAAAVAEMSQ EAIAELEKNG KYALNLDGAE AVIEAADVEI
1060 1070 1080 1090 1100
FSEDIPGWLV ANEGKLTVAL EVTVTEELRR EGIARELVNR IQNIRKSSGF
1110 1120 1130 1140 1150
EITDKIKITI SKNTQTDDAV NEYNTYICNQ VLGTSLDLAD EVKDGTELNF
1160
DDFSLFVNVI KD
Length:1,162
Mass (Da):132,494
Last modified:June 1, 2003 - v1
Checksum:iB6CEFE096695451B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO75913.1.
RefSeqiNP_809719.1. NC_004663.1.
WP_011107458.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO75913; AAO75913; BT_0806.
GeneIDi1073871.
KEGGibth:BT_0806.
PATRICi21056409. VBIBacThe70966_0824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO75913.1.
RefSeqiNP_809719.1. NC_004663.1.
WP_011107458.1. NC_004663.1.

3D structure databases

ProteinModelPortaliQ8A9K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_0806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO75913; AAO75913; BT_0806.
GeneIDi1073871.
KEGGibth:BT_0806.
PATRICi21056409. VBIBacThe70966_0824.

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ8A9K9.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-815-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiSYI_BACTN
AccessioniPrimary (citable) accession number: Q8A9K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: April 1, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.