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Q8A9K9

- SYI_BACTN

UniProt

Q8A9K9 - SYI_BACTN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei713 – 7131ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBTHE226186:GJXV-815-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BT_0806
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000001414: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11621162Isoleucine--tRNA ligasePRO_0000098522Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226186.BT_0806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8A9K9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi710 – 7145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8A9K9-1 [UniParc]FASTAAdd to Basket

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    MGKRFTEYSQ FDLSQVNKDV LKKWDENQVF AKSMTERDGC PSFVFFEGPP     50
    SANGMPGIHH VMARTIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELSVEK 100
    ALGITKEDIG KKISVADYNA ACRKDVMKYT KEWEDLTHQM GYWVDMKHPY 150
    ITYDNRYIET LWWLLKQLHK KGLLYKGYTI QPYSPAAGTG LSSHELNQPG 200
    CYRDVKDTTA VAQFKMKNPK PEMAEWGTPY FLAWTTTPWT LPSNTALCVG 250
    PKIDYVAVQT YNAYTGEPIT VVLAKALLNT HFNSKAADLK LEDYKAGDKL 300
    VPFKVVAEYK GADLIGMEYE QLIPWVKPVE VSEDGTWKVS GKGFRVIPGD 350
    YVTTEDGTGI VHIAPTFGAD DANVARAAGI PSLFMINKKG ETRPMVDLTG 400
    KFYMLDELDE NFVKECVDVD KYKEYQGAWV KNAYNPVFMV DGKYDEKAAQ 450
    AAESLDVALC MMMKANNQAF KIEKHIHNYP HCWRTDKPVL YYPLDSWFIR 500
    STACKERMME LNKTINWKPE STGTGRFGKW LENLNDWNLS RSRYWGTPLP 550
    IWRTEDGTSE ICIESVEELY NEIEKSVAAG FMKSNPYKDK GFVPGEYTEG 600
    NYDKIDLHRP YVDDIILVSE DGQPMKRESD LIDVWFDSGA MPYAQIHYPF 650
    ENKNILDNRE VYPADFIAEG VDQTRGWFFT LHAIATMVFD SVSYKAVISN 700
    GLVLDKNGNK MSKRLNNAVD PFTTIEKYGS DPLRWYMITN SSPWDNLKFD 750
    IDGIEEVRRK FFGTLYNTYS FFALYANVDG FEYKEADVPM AERPEIDRWI 800
    LSVLNTLIKE VDTCYNEYEP TKAGRLISDF VNDNLSNWYV RLNRKRFWGG 850
    EFTQDKLSAY QTLYTCLETV AKLMAPISPF YADRLYTDLT TATGRDNVVS 900
    IHLAEFPKYQ EEMIDKELEA RMQMAQDVTS MVLALRRKVN IKVRQPLQCI 950
    MIPVADEEQK AHIEAVKALI MNEVNVKDIK FVDGAAGVLV KKVKCDFKKL 1000
    GPKFGKQMKA VAAAVAEMSQ EAIAELEKNG KYALNLDGAE AVIEAADVEI 1050
    FSEDIPGWLV ANEGKLTVAL EVTVTEELRR EGIARELVNR IQNIRKSSGF 1100
    EITDKIKITI SKNTQTDDAV NEYNTYICNQ VLGTSLDLAD EVKDGTELNF 1150
    DDFSLFVNVI KD 1162
    Length:1,162
    Mass (Da):132,494
    Last modified:June 1, 2003 - v1
    Checksum:iB6CEFE096695451B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO75913.1.
    RefSeqiNP_809719.1. NC_004663.1.
    WP_011107458.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO75913; AAO75913; BT_0806.
    GeneIDi1073871.
    KEGGibth:BT_0806.
    PATRICi21056409. VBIBacThe70966_0824.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO75913.1 .
    RefSeqi NP_809719.1. NC_004663.1.
    WP_011107458.1. NC_004663.1.

    3D structure databases

    ProteinModelPortali Q8A9K9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226186.BT_0806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO75913 ; AAO75913 ; BT_0806 .
    GeneIDi 1073871.
    KEGGi bth:BT_0806.
    PATRICi 21056409. VBIBacThe70966_0824.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BTHE226186:GJXV-815-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
      Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
      Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

    Entry informationi

    Entry nameiSYI_BACTN
    AccessioniPrimary (citable) accession number: Q8A9K9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3