Q8A9K9 (SYI_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	BT_0806

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	1162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003
Cofactor	Zinc By similarity. HAMAP MF_02003
Subunit structure	Monomer By similarity. HAMAP MF_02003
Subcellular location	Cytoplasm By similarity HAMAP MF_02003.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1162	1162	Isoleucine--tRNA ligase HAMAP MF_02003		PRO_0000098522
Regions
	Motif	50 – 60	11	"HIGH" region HAMAP MF_02003
	Motif	710 – 714	5	"KMSKS" region HAMAP MF_02003
Sites
	Binding site	713	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A9K9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B6CEFE096695451B	FASTA	1,162	132,494
	10 20 30 40 50 60 MGKRFTEYSQ FDLSQVNKDV LKKWDENQVF AKSMTERDGC PSFVFFEGPP SANGMPGIHH 70 80 90 100 110 120 VMARTIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELSVEK ALGITKEDIG KKISVADYNA 130 140 150 160 170 180 ACRKDVMKYT KEWEDLTHQM GYWVDMKHPY ITYDNRYIET LWWLLKQLHK KGLLYKGYTI 190 200 210 220 230 240 QPYSPAAGTG LSSHELNQPG CYRDVKDTTA VAQFKMKNPK PEMAEWGTPY FLAWTTTPWT 250 260 270 280 290 300 LPSNTALCVG PKIDYVAVQT YNAYTGEPIT VVLAKALLNT HFNSKAADLK LEDYKAGDKL 310 320 330 340 350 360 VPFKVVAEYK GADLIGMEYE QLIPWVKPVE VSEDGTWKVS GKGFRVIPGD YVTTEDGTGI 370 380 390 400 410 420 VHIAPTFGAD DANVARAAGI PSLFMINKKG ETRPMVDLTG KFYMLDELDE NFVKECVDVD 430 440 450 460 470 480 KYKEYQGAWV KNAYNPVFMV DGKYDEKAAQ AAESLDVALC MMMKANNQAF KIEKHIHNYP 490 500 510 520 530 540 HCWRTDKPVL YYPLDSWFIR STACKERMME LNKTINWKPE STGTGRFGKW LENLNDWNLS 550 560 570 580 590 600 RSRYWGTPLP IWRTEDGTSE ICIESVEELY NEIEKSVAAG FMKSNPYKDK GFVPGEYTEG 610 620 630 640 650 660 NYDKIDLHRP YVDDIILVSE DGQPMKRESD LIDVWFDSGA MPYAQIHYPF ENKNILDNRE 670 680 690 700 710 720 VYPADFIAEG VDQTRGWFFT LHAIATMVFD SVSYKAVISN GLVLDKNGNK MSKRLNNAVD 730 740 750 760 770 780 PFTTIEKYGS DPLRWYMITN SSPWDNLKFD IDGIEEVRRK FFGTLYNTYS FFALYANVDG 790 800 810 820 830 840 FEYKEADVPM AERPEIDRWI LSVLNTLIKE VDTCYNEYEP TKAGRLISDF VNDNLSNWYV 850 860 870 880 890 900 RLNRKRFWGG EFTQDKLSAY QTLYTCLETV AKLMAPISPF YADRLYTDLT TATGRDNVVS 910 920 930 940 950 960 IHLAEFPKYQ EEMIDKELEA RMQMAQDVTS MVLALRRKVN IKVRQPLQCI MIPVADEEQK 970 980 990 1000 1010 1020 AHIEAVKALI MNEVNVKDIK FVDGAAGVLV KKVKCDFKKL GPKFGKQMKA VAAAVAEMSQ 1030 1040 1050 1060 1070 1080 EAIAELEKNG KYALNLDGAE AVIEAADVEI FSEDIPGWLV ANEGKLTVAL EVTVTEELRR 1090 1100 1110 1120 1130 1140 EGIARELVNR IQNIRKSSGF EITDKIKITI SKNTQTDDAV NEYNTYICNQ VLGTSLDLAD 1150 1160 EVKDGTELNF DDFSLFVNVI KD

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO75913.1.
RefSeq	NP_809719.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A9K9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1073871.
GenomeReviews	Gene locus BT_0806 in contig AE015928_GR.
KEGG	bth:BT_0806.
NMPDR	fig|226186.1.peg.806.
PATRIC	21056409. VBIBacThe70966_0824.
Phylogenomic databases
HOGENOM	HBG577712.
OMA	PYITYDN.
PhylomeDB	Q8A9K9.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_0806-MONOMER.
Family and domain databases
HAMAP	MF_02003. Ile_tRNA_synth_type2. [Tree]
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt. IPR023586. Ile-tRNA-synt_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01870.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. IleS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BACTN

Accession

Primary (citable) accession number: Q8A9K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program