Reviewed, UniProtKB/Swiss-Prot Q8A9C2 (TRMD_BACTN)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BT_0893

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060330
Regions
	Region	132 – 137	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	112	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A9C2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0B49CF62E2AC9E71	FASTA	225	25,558
	10 20 30 40 50 60 MRIDIITVLP EMIEGFFNCS IMKRAQNKGL AEIHIHNLRD YTEDKYRRVD DYPFGGFAGM 70 80 90 100 110 120 VMKIEPIERC INALKAERDY DEVIFTTPDG EQFNQPMANS LSLAQNLIIL CGHFKGIDYR 130 140 150 160 170 180 IREHLITKEI SIGDYVLTGG ELAAAVMADA IVRIIPGVIS DEQSALSDSF QDNLLAAPVY 190 200 210 220 TRPADYKGWK VPDILLSGHE AKIKEWELQQ SLERTKKLRP DLLED

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO76000.1.
RefSeq	NP_809806.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1073262.
GenomeReviews	Gene locus BT_0893 in contig AE015928_GR.
KEGG	bth:BT_0893.
NMPDR	fig|226186.1.peg.893.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A9C2.
OMA	Q8A9C2. HRSVDDT.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_0893-MON.
BRENDA	2.1.1.31. 21018.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BACTN

Accession

Primary (citable) accession number: Q8A9C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)