Reviewed, UniProtKB/Swiss-Prot Q8A988 (SYP_BACTN)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BT_0929

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Prolyl-tRNA synthetase HAMAP MF_01571		PRO_0000249123

Sequences

	Sequence		Length	Mass (Da)
	Q8A988-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5960D3C9344E11FA	FASTA	497	56,848
	10 20 30 40 50 60 MAKELKDLTK RSENYSQWYN DLVVKADLAE QSAVRGCMVI KPYGYAIWEK MQRQLDDMFK 70 80 90 100 110 120 ETGHVNAYFP LLIPKSFLSR EAEHVEGFAK ECAVVTHYRL KNAEDGSGVV VDPAAKLEEE 130 140 150 160 170 180 LIIRPTSETI IWNTYKNWIQ SYRDLPILCN QWANVFRWEM RTRLFLRTAE FLWQEGHTAH 190 200 210 220 230 240 ATREEAEEEA IRMLNVYGEF AEKYMAVPVV KGVKSANERF AGALDTYTIE AMMQDGKALQ 250 260 270 280 290 300 SGTSHFLGQN FAKAFDVQFV NKENKMEYVW ATSWGVSTRL MGALIMTHSD DNGLVLPPHL 310 320 330 340 350 360 APIQVVIVPI YKNDEQLKQI DAKVEGIVAK LKALGISVKY DNADNKRPGF KFADYELKGV 370 380 390 400 410 420 PVRLVMGGRD LENNTMEVMR RDTLEKETVT CDGIETYVQK LLEEIQANIY KKALDYRNSK 430 440 450 460 470 480 ITTVDTYEEF KEKIEEGGFI LAHWDGTTET EEKIKEDTKA TIRCIPFDSY VEGDKEPGKC 490 MVTGKPSACR VVFARSY

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO76036.1.
RefSeq	NP_809842.1.
3D structure databases
HSSP	HSSP built from PDB template 1HC7 based on UniProtKB Q93N97.
ModBase	Search...
Genome annotation databases
GeneID	1073381.
GenomeReviews	Gene locus BT_0929 in contig AE015928_GR.
KEGG	bth:BT_0929.
NMPDR	fig|226186.1.peg.929.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A988.
OMA	CIEAMMQ.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_0929-MON.
BRENDA	6.1.1.15. 21018.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACTN

Accession

Primary (citable) accession number: Q8A988

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)