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Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA1

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
Active sitei130 – 1301Proton acceptorUniRule annotation
Binding sitei157 – 1571Allosteric activator ADPUniRule annotation
Binding sitei165 – 1651Substrate; shared with dimeric partnerUniRule annotation
Binding sitei214 – 2141Allosteric activator ADPUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 752ATPUniRule annotation
Nucleotide bindingi104 – 1074ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-1121-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:pfkA1UniRule annotation
Ordered Locus Names:BT_1102
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000001414 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327ATP-dependent 6-phosphofructokinase 1PRO_0000111937Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_1102.

Structurei

3D structure databases

ProteinModelPortaliQ8A8R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 275Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni128 – 1303Substrate bindingUniRule annotation
Regioni172 – 1743Substrate bindingUniRule annotation
Regioni188 – 1903Allosteric activator ADP bindingUniRule annotation
Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
Regioni257 – 2604Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
InParanoidiQ8A8R5.
KOiK00850.
OMAiIVIVTES.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8A8R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNKYIGILT SGGDASGMNA AIRAVTRAAI FNGFKVKGIY RGYEGLIAGE
60 70 80 90 100
VKELTTEDVS SIIQRGGTIL KTARSETFTT PEGRKKAYKV IQKENINALI
110 120 130 140 150
IIGGDGSLTG ARIFAEEYDV TCIGLPGTID NDLYGTDFTI GYDTALNTIV
160 170 180 190 200
ECVDKIRDTA TSHDRIFFVE VMGRDAGFLA QNSAIASGAE AAIIPEDRTD
210 220 230 240 250
VDQLETFIGR GFRKTKNSSI VIVTESPENK NGGAIYYADR VKKEYPGYDV
260 270 280 290 300
RVSILGHLQR GGAPSANDRI LASRLGEAAI QALMEGQRNV MIGIRNNEIV
310 320
YVPFVQAIKK DKPIDKSLIR VLNELSI
Length:327
Mass (Da):35,511
Last modified:June 16, 2003 - v1
Checksum:iAD8900CDDF1B5479
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO76209.1.
RefSeqiNP_810015.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO76209; AAO76209; BT_1102.
GeneIDi1073299.
KEGGibth:BT_1102.
PATRICi21057033. VBIBacThe70966_1126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO76209.1.
RefSeqiNP_810015.1. NC_004663.1.

3D structure databases

ProteinModelPortaliQ8A8R5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_1102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO76209; AAO76209; BT_1102.
GeneIDi1073299.
KEGGibth:BT_1102.
PATRICi21057033. VBIBacThe70966_1126.

Phylogenomic databases

eggNOGiCOG0205.
InParanoidiQ8A8R5.
KOiK00850.
OMAiIVIVTES.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciBTHE226186:GJXV-1121-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiPFKA1_BACTN
AccessioniPrimary (citable) accession number: Q8A8R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: April 29, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.