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Q8A8R5

- PFKA1_BACTN

UniProt

Q8A8R5 - PFKA1_BACTN

Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA1

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (16 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131ATP; via amide nitrogenUniRule annotation
    Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
    Active sitei130 – 1301Proton acceptorUniRule annotation
    Binding sitei157 – 1571Allosteric activator ADPUniRule annotation
    Binding sitei165 – 1651Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei214 – 2141Allosteric activator ADPUniRule annotation
    Binding sitei225 – 2251SubstrateUniRule annotation
    Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi74 – 752ATPUniRule annotation
    Nucleotide bindingi104 – 1074ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBTHE226186:GJXV-1121-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 1UniRule annotation
    Short name:
    Phosphofructokinase 1UniRule annotation
    Alternative name(s):
    Phosphohexokinase 1UniRule annotation
    Gene namesi
    Name:pfkA1UniRule annotation
    Ordered Locus Names:BT_1102
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000001414: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327ATP-dependent 6-phosphofructokinase 1PRO_0000111937Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226186.BT_1102.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8A8R5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni23 – 275Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni128 – 1303Substrate bindingUniRule annotation
    Regioni172 – 1743Substrate bindingUniRule annotation
    Regioni188 – 1903Allosteric activator ADP bindingUniRule annotation
    Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
    Regioni257 – 2604Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiNVMIGIR.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8A8R5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNKYIGILT SGGDASGMNA AIRAVTRAAI FNGFKVKGIY RGYEGLIAGE    50
    VKELTTEDVS SIIQRGGTIL KTARSETFTT PEGRKKAYKV IQKENINALI 100
    IIGGDGSLTG ARIFAEEYDV TCIGLPGTID NDLYGTDFTI GYDTALNTIV 150
    ECVDKIRDTA TSHDRIFFVE VMGRDAGFLA QNSAIASGAE AAIIPEDRTD 200
    VDQLETFIGR GFRKTKNSSI VIVTESPENK NGGAIYYADR VKKEYPGYDV 250
    RVSILGHLQR GGAPSANDRI LASRLGEAAI QALMEGQRNV MIGIRNNEIV 300
    YVPFVQAIKK DKPIDKSLIR VLNELSI 327
    Length:327
    Mass (Da):35,511
    Last modified:June 16, 2003 - v1
    Checksum:iAD8900CDDF1B5479
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76209.1.
    RefSeqiNP_810015.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO76209; AAO76209; BT_1102.
    GeneIDi1073299.
    KEGGibth:BT_1102.
    PATRICi21057033. VBIBacThe70966_1126.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76209.1 .
    RefSeqi NP_810015.1. NC_004663.1.

    3D structure databases

    ProteinModelPortali Q8A8R5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226186.BT_1102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO76209 ; AAO76209 ; BT_1102 .
    GeneIDi 1073299.
    KEGGi bth:BT_1102.
    PATRICi 21057033. VBIBacThe70966_1126.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi NVMIGIR.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci BTHE226186:GJXV-1121-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
      Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
      Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

    Entry informationi

    Entry nameiPFKA1_BACTN
    AccessioniPrimary (citable) accession number: Q8A8R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3