Reviewed, UniProtKB/Swiss-Prot Q8A8L4 (SERC_BACTN)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	BT_1153

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Phosphoserine aminotransferase HAMAP MF_00160		PRO_0000150154
Regions
	Region	75 – 76	2	Pyridoxal phosphate binding By similarity
	Region	231 – 232	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	41	1	L-glutamate By similarity
	Binding site	99	1	Pyridoxal phosphate By similarity
	Binding site	147	1	Pyridoxal phosphate By similarity
	Binding site	166	1	Pyridoxal phosphate By similarity
	Binding site	189	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	190	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A8L4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D6FE0762EF14968F	FASTA	355	39,434
	10 20 30 40 50 60 MKKHNFNAGP SILPREVIED TAKAILDFNG SGLSLMEISH RAKDFQPVVD EAEALFKELL 70 80 90 100 110 120 NIPEGYSVLF LGGGASMEFC MVPFNFLEKK AAYLNTGVWA KKAMKEAKGF GEVVEVASSA 130 140 150 160 170 180 EATYTYIPKD YTIPADADYF HITTNNTIYG TELKEDLNSP VPMVADMSSD IFSRPIDVSK 190 200 210 220 230 240 YICIYGGAQK NLAPAGVTFV IVKNDAVGKV SRYIPSMLNY QTHIDNGSMF NTPPVVPIYA 250 260 270 280 290 300 ALLNLRWIKA QGGVKEMERR AIEKADMLYA EIDRNKLFVG TAAKEDRSRM NICFVMAPEY 310 320 330 340 350 KDLEADFMKF ATEKGMVGIK GHRSVGGFRA SCYNALPKES VQALIDCMQE FEKLH

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO76260.1.
RefSeq	NP_810066.1.
3D structure databases
HSSP	HSSP built from PDB template 1BT4 based on UniProtKB Q59196.
ModBase	Search...
Genome annotation databases
GeneID	1073773.
GenomeReviews	Gene locus BT_1153 in contig AE015928_GR.
KEGG	bth:BT_1153.
NMPDR	fig|226186.1.peg.1153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A8L4.
OMA	SMYNTPP.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_1153-MON.
BRENDA	2.6.1.52. 21018.
Family and domain databases
HAMAP	MF_00160. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_BACTN

Accession

Primary (citable) accession number: Q8A8L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)