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Protein

L-Ala-D/L-Glu epimerase

Gene

BT_1313

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and may play a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp, L-Ala-L-Glu, L-Ala-L-Ser, L-Ala-L-Pro, L-Ala-L-L-Val, L-Ala-L-Thr, L-Ala-L-Leu, L-Ala-L-Ile and L-Gly-L-Glu (in vitro).1 Publication

Catalytic activityi

L-alanyl-D-glutamate = L-alanyl-L-glutamate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

kcat is 147 sec(-1) for epimerization of L-Ala-L-Glu. kcat is 59 sec(-1) for epimerization of L-Ala-D-Glu. kcat is 96 sec(-1) for epimerization of L-Val-L-Glu. kcat is 43 sec(-1) for epimerization of L-Val-D-Glu. kcat is 30 sec(-1) for epimerization of L-Ile-L-Glu.

  1. KM=2.0 mM for L-Ala-L-Glu1 Publication
  2. KM=6.0 mM for L-Ala-D-Glu1 Publication
  3. KM=1.7 mM for L-Val-L-Glu1 Publication
  4. KM=4.4 mM for L-Val-D-Glu1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei68Substrate1
    Binding sitei94Substrate1
    Metal bindingi224Magnesium1 Publication1
    Metal bindingi251Magnesium1 Publication1
    Metal bindingi276Magnesium1 Publication1
    Binding sitei298Substrate1

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • racemase and epimerase activity Source: UniProtKB

    GO - Biological processi

    • cell wall organization Source: UniProtKB-KW
    • peptide metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-Ala-D/L-Glu epimerase (EC:5.1.1.20)
    Short name:
    AE epimerase
    Short name:
    AEE
    Gene namesi
    Ordered Locus Names:BT_1313
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    Proteomesi
    • UP000001414 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004296461 – 383L-Ala-D/L-Glu epimeraseAdd BLAST383

    Proteomic databases

    PaxDbiQ8A861.

    Interactioni

    Protein-protein interaction databases

    STRINGi226186.BT_1313.

    Structurei

    Secondary structure

    1383
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi48 – 52Combined sources5
    Beta strandi55 – 63Combined sources9
    Beta strandi66 – 71Combined sources6
    Beta strandi73 – 80Combined sources8
    Beta strandi83 – 90Combined sources8
    Helixi93 – 95Combined sources3
    Helixi99 – 106Combined sources8
    Helixi120 – 129Combined sources10
    Helixi135 – 153Combined sources19
    Helixi157 – 160Combined sources4
    Helixi165 – 167Combined sources3
    Helixi180 – 191Combined sources12
    Beta strandi195 – 200Combined sources6
    Beta strandi202 – 204Combined sources3
    Helixi206 – 214Combined sources9
    Beta strandi221 – 224Combined sources4
    Helixi232 – 244Combined sources13
    Beta strandi247 – 251Combined sources5
    Helixi256 – 258Combined sources3
    Helixi259 – 267Combined sources9
    Beta strandi273 – 276Combined sources4
    Helixi282 – 284Combined sources3
    Helixi286 – 288Combined sources3
    Turni289 – 291Combined sources3
    Beta strandi292 – 297Combined sources6
    Helixi299 – 302Combined sources4
    Helixi305 – 317Combined sources13
    Beta strandi321 – 324Combined sources4
    Helixi331 – 338Combined sources8
    Helixi339 – 343Combined sources5
    Beta strandi345 – 347Combined sources3
    Helixi351 – 354Combined sources4
    Beta strandi364 – 366Combined sources3
    Beta strandi369 – 371Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3IJIX-ray1.60A/B46-383[»]
    3IJLX-ray1.50A/B46-383[»]
    3IJQX-ray2.00A/B46-383[»]
    ProteinModelPortaliQ8A861.
    SMRiQ8A861.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni198 – 200Substrate binding3
    Regioni326 – 328Substrate binding3
    Regioni348 – 350Substrate binding3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105DTQ. Bacteria.
    COG4948. LUCA.
    InParanoidiQ8A861.
    KOiK19802.
    OMAiRALDMKV.
    OrthoDBiPOG091H04PO.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF13378. MR_MLE_C. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8A861-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPNRRDFLKT AAFATLGSGI AVSQVLAGEC MPSAIHINKY GIGGKMKMTF
    60 70 80 90 100
    FPYELKLRHV FTVATYSRTT TPDVQVEIEY EGVTGYGEAS MPPYLGETVE
    110 120 130 140 150
    SVMNFLKKVN LEQFSDPFQL EDILSYVDSL SPKDTAAKAA VDIALHDLVG
    160 170 180 190 200
    KLLGAPWYKI WGLNKEKTPS TTFTIGIDTP DVVRAKTKEC AGLFNILKVK
    210 220 230 240 250
    LGRDNDKEMI ETIRSVTDLP IAVDANQGWK DRQYALDMIH WLKEKGIVMI
    260 270 280 290 300
    EQPMPKEQLD DIAWVTQQSP LPVFADESLQ RLGDVAALKG AFTGINIKLM
    310 320 330 340 350
    KCTGMREAWK MVTLAHALGM RVMVGCMTET SCAISAASQF SPAVDFADLD
    360 370 380
    GNLLISNDRF KGVEVVNGKI TLNDLPGIGV MKI
    Length:383
    Mass (Da):42,168
    Last modified:June 1, 2003 - v1
    Checksum:i1088235F1C0A356F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76420.1.
    RefSeqiNP_810226.1. NC_004663.1.
    WP_011107704.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO76420; AAO76420; BT_1313.
    GeneIDi1073218.
    KEGGibth:BT_1313.
    PATRICi21057481. VBIBacThe70966_1342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76420.1.
    RefSeqiNP_810226.1. NC_004663.1.
    WP_011107704.1. NC_004663.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3IJIX-ray1.60A/B46-383[»]
    3IJLX-ray1.50A/B46-383[»]
    3IJQX-ray2.00A/B46-383[»]
    ProteinModelPortaliQ8A861.
    SMRiQ8A861.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi226186.BT_1313.

    Proteomic databases

    PaxDbiQ8A861.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAO76420; AAO76420; BT_1313.
    GeneIDi1073218.
    KEGGibth:BT_1313.
    PATRICi21057481. VBIBacThe70966_1342.

    Phylogenomic databases

    eggNOGiENOG4105DTQ. Bacteria.
    COG4948. LUCA.
    InParanoidiQ8A861.
    KOiK19802.
    OMAiRALDMKV.
    OrthoDBiPOG091H04PO.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF13378. MR_MLE_C. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAEEP_BACTN
    AccessioniPrimary (citable) accession number: Q8A861
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 9, 2014
    Last sequence update: June 1, 2003
    Last modified: November 2, 2016
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Part of a large, functionally divergent protein family. Protein modeling and substrate docking were used to predict the substrate specificity, prior to biochemical analysis.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.